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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

PTK6 Gene

protein-coding GIFtS: 70
GCID: GC20M062159

Protein Tyrosine Kinase 6

(Previous name: PTK6 protein tyrosine kinase 6)

See related diseases
at

MalaCards

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

Aliases
Protein Tyrosine Kinase 6¹ ²		EC 2.7.10.2³ ⁸
PTK6 Protein Tyrosine Kinase 6¹ ²		Protein-Tyrosine Kinase 6²
Breast Tumor Kinase² ³		Protein-Tyrosine Kinase BRK²
Tyrosine-Protein Kinase BRK² ³		EC 2.7.10⁸
BRK² ³

External Ids:	HGNC: 9617¹	Entrez Gene: 5753²	Ensembl: ENSG00000101213⁷	OMIM: 602004⁵	UniProtKB: Q13882³

Export aliases for PTK6 gene to outside databases

Previous GC identifers: GC20M062037 GC20M061997 GC20M062839 GC20M062886 GC20M061630 GC20M058889

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for PTK6 Gene:

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an

intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells

leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype.

Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue.

The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple

transcript variants. (provided by RefSeq, Jan 2012)

GeneCards Summary for PTK6 Gene:

PTK6 (protein tyrosine kinase 6) is a protein-coding gene. Diseases associated with PTK6 include band keratopathy, and recurrent corneal erosion, and among its related super-pathways are Focal adhesion and Cellular Effects of Sildenafil. GO annotations related to this gene include non-membrane spanning protein tyrosine kinase activity and protein serine/threonine kinase activity. An important paralog of this gene is FRK.

UniProtKB/Swiss-Prot: PTK6_HUMAN, Q13882

Function: Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways

that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to

be context dependent and differ depending on cell type, as well as its intracellular localization. A number of

potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins:

KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety

of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of

proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an

adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic

tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression

by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and

migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth

through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and

proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association

with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of

PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6

activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in

normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways

Function: Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins

Gene Wiki entry for PTK6 Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000020.10 NT_011333.6 NC_018931.2

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the PTK6 gene promoter:
AREB6 S8 AP-1 NRSF form 1 ATF-2 ARP-1 Pax-2a c-Jun NRSF form 2
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: PTK6 promoter sequence

Search SABiosciences Chromatin IP Primers for PTK6

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PTK6

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20q13.3 Ensembl cytogenetic band: 20q13.33 HGNC cytogenetic band: 20q13.3

PTK6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PTK6 gene location

GeneLoc information about chromosome 20 GeneLoc Exon Structure

GeneLoc location for GC20M062159: view genomic region (about GC identifiers)

Start:	62,159,766 bp from pter	End:	62,168,723 bp from pter
Size:	8,958 bases	Orientation:	minus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: PTK6_HUMAN, Q13882 (See protein sequence)

Recommended Name: Protein-tyrosine kinase 6

Size: 451 amino acids; 51834 Da

Subunit: Interacts with GAP-A.p65 (By similarity). Interacts (via SH3 and SH2 domains) with KHDRBS1. Interacts

(via SH3 and SH2 domains) with phosphorylated IRS4. Interacts with ADAM15. Interacts (via SH3 domain) with SFPQ.

Interacts with EGFR and ERBB2. Interacts with STAP2. Interacts with PNX. Interacts with SFPQ. Interacts with

PTK/ATK. Interacts with CTNNB1

Subcellular location: Cytoplasm. Nucleus. Cell projection, ruffle. Membrane (By similarity). Note=Colocalizes with

KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. Nuclear localization in epithelial cells of normal prostate but

cytoplasmic localization in cancer prostate

Sequence caution: Sequence=BAG62908.1; Type=Erroneous translation; Note=Wrong choice of CDS;

2 PDB 3D structures from and Proteopedia for PTK6:

1RJA (3D)

2KGT (3D)

Secondary accessions: B2RCR3 B4DW46 Q58F01

Alternative splicing: 2 isoforms: Q13882-1 Q13882-2

Explore the universe of human proteins at neXtProt for PTK6: NX_Q13882

Explore proteomics data for PTK6 at MOPED

Post-translational modifications:

UniProtKB: Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the

SH2 domain when phosphorylated and negatively regulates kinase activity

View modification sites using PhosphoSitePlus

View neXtProt modification sites for NX_Q13882

3 DME Specific Peptides for PTK6 (Q13882)

KWTAPEA

RDLAARN

KSDVWSFG

PTK6 Protein expression data from MOPED¹, PaxDb² and MAXQB³ : About this image

REFSEQ proteins (2 alternative transcripts):

NP_001243287.1 NP_005966.1

ENSEMBL proteins:

ENSP00000217185 ENSP00000442460

Human Recombinant Protein Products for PTK6:

	EMD Millipore Purified and/or Recombinant PTK6 Protein
	Browse R&D Systems for human recombinant proteins
	Enzo Life Sciences proteins for PTK6
	OriGene Purified Protein for PTK6 OriGene Protein Over-expression Lysate for PTK6 OriGene MassSpec for PTK6 OriGene Custom Protein Services for PTK6
	GenScript Custom Purified and Recombinant Proteins Services for PTK6
	Novus Biologicals PTK6 Proteins Novus Biologicals PTK6 Lysates
	Sino Biological Recombinant Protein for PTK6 Sino Biological Cell Lysate for PTK6
	Browse ProSpec Recombinant Proteins
	Cloud-Clone Corp. Proteins for PTK6

Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001726	ruffle	IDA	15572663
GO:0005634	nucleus	IDA	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005737	cytoplasm	IDA	15572663
GO:0005886	plasma membrane	IDA	--

PTK6 for ontologies About GeneDecksing

PTK6 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of PTK6
	R&D Systems Antibodies for PTK6 (Brk)
	Browse OriGene Antibodies OriGene Custom Antibody Services for PTK6
	GenScript Custom Superior Antibodies Services for PTK6
	Novus Biologicals PTK6 Antibodies
	Abcam antibodies for PTK6
	Cloud-Clone Corp. Antibodies for PTK6
	ThermoFisher Antibodies for PTK6
	LSBio Antibodies in human, mouse, rat for PTK6

Assay Products for PTK6:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for PTK6
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for PTK6
	Browse Enzo Life Sciences for kits & assays
	Cloud-Clone Corp. ELISAs for PTK6 Cloud-Clone Corp. CLIAs for PTK6

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

HGNC Gene Families:

SH2D: SH2 domain containing

IUPHAR Guide to PHARMACOLOGY protein family classification: PTK6 protein tyrosine kinase 6

Src family

5/8 InterPro protein domains (see all 8):

IPR017441 Protein_kinase_ATP_BS

IPR011009 Kinase-like_dom

IPR001452 SH3_domain

IPR000719 Prot_kinase_cat_dom

IPR001245 Ser-Thr/Tyr_kinase_cat_dom

Graphical View of Domain Structure for InterPro Entry Q13882

ProtoNet protein and cluster: Q13882

3 Blocks protein domains:

IPB000980 SH2 domain signature

IPB001452 SH3 domain signature

IPB008266 Tyrosine protein kinase

UniProtKB/Swiss-Prot: PTK6_HUMAN, Q13882

Domain: The SH3 domain plays a major role in substrate interactions. The SH2 domain of PTK6 plays a role in

protein-protein interactions, but is likely more important for the regulation of catalytic activity

Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily

Similarity: Contains 1 protein kinase domain

Similarity: Contains 1 SH2 domain

Similarity: Contains 1 SH3 domain

PTK6 for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: PTK6_HUMAN, Q13882