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PSKH2 Gene

protein-coding   GIFtS: 44
GCID: GC08M087060

Protein Serine Kinase H2

  Search for PSKH2
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Protein Serine Kinase H21 2 3
PSK-H22 3
EC 2.7.11.13 8
Serine/Threonine Kinase PSKH22
Serine/Threonine-Protein Kinase H22
EC 2.7.118

External Ids:    HGNC: 189971   Entrez Gene: 854812   Ensembl: ENSG000001476137   UniProtKB: Q96QS63   

Export aliases for PSKH2 gene to outside databases

Previous GC identifers: GC08M085649 GC08M086792 GC08M087017 GC08M087129 GC08M082270


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for PSKH2 Gene:
PSKH2 (protein serine kinase H2) is a protein-coding gene. GO annotations related to this gene include protein serine/threonine kinase activity. An important paralog of this gene is PNCK.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Regulatory transcription factor binding sites in the PSKH2 gene promoter:
         SRF   STAT1   SRF (504 AA)   STAT1beta   STAT1alpha   GATA-2   S8   POU2F1   POU2F1a   Pax-4a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidPSKH2 promoter sequence
   Search Chromatin IP Primers for PSKH2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PSKH2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q21.3   Ensembl cytogenetic band:  8q21.3   HGNC cytogenetic band: 8q21.13

PSKH2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PSKH2 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M087060:  view genomic region     (about GC identifiers)

Start:
87,060,602 bp from pter      End:
87,100,850 bp from pter
Size:
40,249 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: KPSH2_HUMAN, Q96QS6 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase H2  
Size: 385 amino acids; 43027 Da
Caution: Asn-183 is present instead of the conserved Asp which is expected to be an active site residue
Secondary accessions: A0AV22

Explore the universe of human proteins at neXtProt for PSKH2: NX_Q96QS6

Explore proteomics data for PSKH2 at MOPED

Selected DME Specific Peptides for PSKH2 (Q96QS6) (see all 10)
 YIAPEVL  GGELFDR  LKPENLL  RAKFDPRV 


See PSKH2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_149117.1  
ENSEMBL proteins: 
 ENSP00000276616  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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IUPHAR Guide to PHARMACOLOGY protein family classification: protein serine kinase H2
PSK family

5 InterPro protein domains:
 IPR017441 Protein_kinase_ATP_BS
 IPR002290 Ser/Thr_dual-sp_kinase_dom
 IPR011009 Kinase-like_dom
 IPR000719 Prot_kinase_dom
 IPR020636 Ca/CaM-dep_Ca-dep_prot_Kinase

Graphical View of Domain Structure for InterPro Entry Q96QS6

ProtoNet protein and cluster: Q96QS6

UniProtKB/Swiss-Prot: KPSH2_HUMAN, Q96QS6
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family
Similarity: Contains 1 protein kinase domain


PSKH2 for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Molecular Function:

     UniProtKB/Swiss-Prot Summary: KPSH2_HUMAN, Q96QS6
Catalytic activity: ATP + a protein = ADP + a phosphoprotein

     Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112

     Gene Ontology (GO): 5 molecular function terms:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0004672protein kinase activity ----
GO:0004674protein serine/threonine kinase activity IEA--
GO:0004713protein tyrosine kinase activity ----
GO:0005524ATP binding IEA--
GO:0016772transferase activity, transferring phosphorus-containing groups ----
     
PSKH2 for ontologies           About GeneDecksing


Phenotypes:
     4 GenomeRNAi human phenotypes for PSKH2:
 Decreased focal adhesion (FA)   Increased cell death HMECs cel  Increased colony dispersion (i  Synthetic lethal with Ras 

Animal Models:
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
cytosol2
mitochondrion1
nucleus1

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for PSKH2
Interactions:

    Search GeneGlobe Interaction Network for PSKH2

STRING Interaction Network Preview (showing 5 interactants - click image to see 6)

Selected Interacting proteins for PSKH2 (ENSP000002766164) via UniProtKB, MINT, STRING, and/or I2D (see all 6)
InteractantInteraction Details
GeneCardExternal ID(s)
RAD1ENSP000003408794STRING: ENSP00000340879
MAP2K1ENSP000003024864STRING: ENSP00000302486
MAP2K5ENSP000001786404STRING: ENSP00000178640
HORMAD1ENSP000003551674STRING: ENSP00000355167
HORMAD2ENSP000003369844STRING: ENSP00000336984
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Gene Ontology (GO): 1 biological process term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0006468protein phosphorylation ----

PSKH2 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Tocris compounds for PSKH2 (KPSH2)

2 HMDB Compounds for PSKH2    About this table
CompoundSynonyms CAS #PubMed Ids
ADPadenosindiphosphorsaeure (see all 8)58-64-0--
Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for PSKH2 gene: 
NM_033126.1  

3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000276616(uc011lfy.2) ENST00000517981 ENST00000523010
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3 AceView cDNA sequences:

AY037806 NM_033126 BX956556 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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PSKH2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: CAGAGGGCCT
PSKH2 Expression
About this image

PSKH2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

PSKH2 Protein Expression
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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PSKH2

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals and fungi.

Orthologs for PSKH2 gene from Selected species (see all 11)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
dog
(Canis familiaris)
Mammalia PSKH21 protein serine kinase H2 86.42(n)
82.51(a)
  487033  XM_544162.2  XP_544162.2 
chicken
(Gallus gallus)
Aves --
Uncharacterized protein
31(a)
many ↔ many
28(3045947-3055174)
lizard
(Anolis carolinensis)
Reptilia --
Uncharacterized protein
35(a)
many ↔ many
GL343756.1(217795-264032)
tropical clawed frog
(Xenopus tropicalis)
Amphibia LOC1004972801 serine/threonine-protein kinase H1-like 67.26(n)
67.55(a)
  100497280  XM_002939157.2  XP_002939203.2 
zebrafish
(Danio rerio)
Actinopterygii PSKH26
protein serine kinase H2
46(a)
1 ↔ 1
12(43471417-43684045) ENSDARG00000024936
worm
(Caenorhabditis elegans)
Secernentea R06A10.43 protein kinase 44(a)   I(1090070-1095795)   --
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CMK16
CMK26
Calmodulin-dependent protein kinase; may play a ro...
Calmodulin-dependent protein kinase; may play a ro...
25(a)
24(a)
many ↔ many
many ↔ many
VI(172537-173877) YFR014C
XV(294777-296120) YOL016C


ENSEMBL Gene Tree for PSKH2 (if available)
TreeFam Gene Tree for PSKH2 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for PSKH2 gene
PNCK2  CAMK1G2  DCX2  DCLK22  CAMK2A2  CAMK1D2  DCLK12  PSKH12  
CAMK42  CAMK2D2  DCLK32  CAMKV2  CAMK2G2  CAMK2B2  CAMK12  
Selected SIMAP similar genes for PSKH2 using alignment to 1 protein entry:     KPSH2_HUMAN(see all similar genes):
PSKH1    CAMK1D    CAMK1    PNCK    CAMK1G    DAPK3
CAMK4    SIK1    CAMK2G    CAMKV    DCLK3    MELK
MYLK    PRKAA1    PRKACB    CASK    RPS6KA3    TTN

PSKH2 for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for PSKH2 (see all 585)    About this table    
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 8 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
VAR_0406184
A lung adenocarcinoma sample4--see VAR_0406182 S I mis40--------
VAR_0406234
A lung adenocarcinoma sample4--see VAR_0406232 K I mis40--------
rs1821649061,2
--87060193(+) ATAATA/TAATAA 1 -- ds50010--------
rs1877754631,2
--87060406(+) TATTGC/TATTTT 1 -- ds50010--------
rs732552161,2
C,F--87060428(+) TACATG/ACTTTA 1 -- ds50011Minor allele frequency- A:0.50WA 2
rs1829483331,2
--87060500(+) GTTAAC/TCATTT 1 -- ds50010--------
rs736885721,2
C,F--87060501(+) TTAATC/TATTTG 1 -- ds50011Minor allele frequency- T:0.50WA 2
rs285765591,2
C,F--87060528(+) GAAAAC/TGAATA 1 -- ds50011Minor allele frequency- T:0.50WA 2
rs1512942201,2
C--87060639(+) CCTAAG/TCATGA 1 -- ds50010--------
rs38022251,2
C,F,A,H--87060672(+) AAAATA/GGTTTT 1 -- ds500132Minor allele frequency- G:0.40NA NS EA WA CSA EU 4149

HapMap Linkage Disequilibrium report for PSKH2 (87060602 - 87100850 bp)

Structural Variations
     Database of Genomic Variants (DGV) 6 variations for PSKH2:    About this table    
Variant IDTypeSubtypePubMed ID
esv268395CNV Insertion20981092
esv273774CNV Insertion20981092
nsv891146CNV Gain21882294
dgv7830n71CNV Gain21882294
nsv891147CNV Gain21882294
nsv428204CNV Gain18775914

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SeqTarget long-range PCR primers for resequencing PSKH2
DNA2.0 Custom Variant and Variant Library Synthesis for PSKH2

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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PSKH2 for disorders           About GeneDecksing

Genetic Association Database (GAD): PSKH2
Human Genome Epidemiology (HuGE) Navigator: PSKH2 (1 document)

Export disorders for PSKH2 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for PSKH2 gene integrated from 10 sources:
(articles sorted by number of sources associating them with PSKH2)
    Utopia: connect your pdf to the dynamic
world of online information

  1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  2. Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition. (PubMed id 22939624)1 Taipale M....Lindquist S. (Cell 2012)
  3. Single-nucleotide polymorphisms, acute rejection, and severity of tubulitis in kidney transplantation, accounting for center-to-center variation. (PubMed id 21085059)4 Israni A....Oetting W.S. (Transplantation 2010)
  4. Patterns of somatic mutation in human cancer genomes. (PubMed id 17344846)2 Greenman C.... Stratton M.R. (Nature 2007)
  5. The protein kinase complement of the human genome. (PubMed id 12471243)1 Manning G.... Sudarsanam S. (Science 2002)
  6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
  7. (PubMed id )2 

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 85481 HGNC: 18997 AceView: PSKH2 Ensembl:ENSG00000147613 euGenes: HUgn85481
ECgene: PSKH2 H-InvDB: PSKH2

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for PSKH2 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for PSKH2 gene:
Search GeneIP for patents involving PSKH2

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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 Search Tocris compounds for PSKH2 (KPSH2)
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 Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

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 inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PSKH2
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GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

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