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Aliases for PRUNE1 Gene

Aliases for PRUNE1 Gene

  • Prune Exopolyphosphatase 1 2 3
  • Drosophila-Related Expressed Sequence 17 3 4
  • DRES-17 3 4
  • HTCD37 3 4
  • DRES17 3 4
  • PRUNE 3 4
  • Prune Homolog (Drosophila) 2
  • Exopolyphosphatase PRUNE1 3
  • Prune Exopolyphosphatase 5
  • Protein Prune Homolog 1 3
  • Protein Prune Homolog 3
  • EC 3.6.1.1 4
  • H-PRUNE 3
  • HPrune 4

External Ids for PRUNE1 Gene

Previous HGNC Symbols for PRUNE1 Gene

  • PRUNE

Summaries for PRUNE1 Gene

Entrez Gene Summary for PRUNE1 Gene

  • This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

GeneCards Summary for PRUNE1 Gene

PRUNE1 (Prune Exopolyphosphatase 1) is a Protein Coding gene. Among its related pathways are Purine metabolism (KEGG).

UniProtKB/Swiss-Prot for PRUNE1 Gene

  • Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRUNE1 Gene

Genomics for PRUNE1 Gene

Regulatory Elements for PRUNE1 Gene

Enhancers for PRUNE1 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH01G151308 2 FANTOM5 Ensembl ENCODE dbSUPER 9.4 +303.5 303546 6.1 CREB3L1 AGO1 ZFP64 DMAP1 FEZF1 YY1 SLC30A9 ZNF143 ZNF263 SP3 MRPL9 GABPB2 SETDB1 GOLPH3L LYSMD1 PRPF3 UBE2D3P3 ZNF687 ADAMTSL4 RFX5
GH01G151193 2 FANTOM5 Ensembl ENCODE dbSUPER 9.3 +189.3 189256 7.7 CREB3L1 DMAP1 YY1 ZNF143 ZNF263 NFYC NR2C1 NBN KDM1A SP7 LYSMD1 SETDB1 BNIPL PSMD4 GOLPH3L PRPF3 UBE2D3P3 TMOD4 MRPL9 RPS10P6
GH01G151142 1.3 ENCODE dbSUPER 11 +136.8 136818 6.1 HDGF PKNOX1 ATF1 FOXA2 ZFP64 ARID4B SIN3A DMAP1 ZNF2 ZNF48 BNIPL GABPB2 SEMA6C PRUNE1 TMOD4 VPS72 PIP5K1A RNU6-884P LYSMD1 C1orf56
GH01G150925 1.1 ENCODE 10.6 -82.1 -82112 2.4 HDGF PKNOX1 MLX CREB3L1 ARNT ZFP64 ARID4B SIN3A DMAP1 ZNF2 HORMAD1 GABPB2 GOLPH3L UBE2D3P3 CTSK MRPL9 SF3B4 SETDB1 VPS45 PRPF3
GH01G151134 0.9 dbSUPER 11 +127.5 127532 2.7 FOXA2 RAD21 ZNF48 RARA FOS ZNF362 CEBPB NFIL3 ZNF579 MIER3 BNIPL GABPB2 SEMA6C TNFAIP8L2 TMOD4 PRUNE1 MINDY1 SCNM1 LYSMD1 RNU6-884P
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around PRUNE1 on UCSC Golden Path with GeneCards custom track

Promoters for PRUNE1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000254393 409 1201 CREB3L1 ZFP64 DMAP1 FEZF1 YY1 ZNF143 ZNF263 SP3 ZNF610 GLIS1

Genomic Location for PRUNE1 Gene

Chromosome:
1
Start:
151,008,391 bp from pter
End:
151,035,713 bp from pter
Size:
27,323 bases
Orientation:
Plus strand

Genomic View for PRUNE1 Gene

Genes around PRUNE1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRUNE1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRUNE1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRUNE1 Gene

Proteins for PRUNE1 Gene

  • Protein details for PRUNE1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q86TP1-PRUNE_HUMAN
    Recommended name:
    Protein prune homolog
    Protein Accession:
    Q86TP1
    Secondary Accessions:
    • B2RCH8
    • B4DFL7
    • Q5SZF9
    • Q659E5
    • Q6P4E0
    • Q8N654
    • Q96JU5
    • Q9C071
    • Q9C072
    • Q9UIV0

    Protein attributes for PRUNE1 Gene

    Size:
    453 amino acids
    Molecular mass:
    50200 Da
    Cofactor:
    Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
    Quaternary structure:
    • Homooligomer. Able to homodimerize via its C-terminal domain. Interacts with NME1. Interacts with GSK3; at focal adhesion complexes where paxillin and vinculin are colocalized.
    SequenceCaution:
    • Sequence=BAB55423.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAG60534.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for PRUNE1 Gene

neXtProt entry for PRUNE1 Gene

Post-translational modifications for PRUNE1 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for PRUNE1 Gene

Domains & Families for PRUNE1 Gene

Protein Domains for PRUNE1 Gene

Suggested Antigen Peptide Sequences for PRUNE1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q86TP1

UniProtKB/Swiss-Prot:

PRUNE_HUMAN :
  • Belongs to the PPase class C family. Prune subfamily.
Family:
  • Belongs to the PPase class C family. Prune subfamily.
genes like me logo Genes that share domains with PRUNE1: view

No data available for Gene Families for PRUNE1 Gene

Function for PRUNE1 Gene

Molecular function for PRUNE1 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=0.91 uM for cAMP {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525}; KM=2.3 uM for cGMP {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525}; Vmax=12.8 pmol/min/ug enzyme with cAMP as substrate {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525}; Vmax=0.8 pmol/min/ug enzyme with cGMP as substrate {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525};
UniProtKB/Swiss-Prot CatalyticActivity:
Diphosphate + H(2)O = 2 phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by magnesium ions and inhibited by manganese ions. Inhibited by dipyridamole, moderately sensitive to IBMX and inhibited by vinpocetine.
UniProtKB/Swiss-Prot Function:
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.

Enzyme Numbers (IUBMB) for PRUNE1 Gene

Gene Ontology (GO) - Molecular Function for PRUNE1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004427 inorganic diphosphatase activity IEA --
GO:0005515 protein binding IPI 10602478
GO:0016462 pyrophosphatase activity IEA --
GO:0016787 hydrolase activity IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with PRUNE1: view
genes like me logo Genes that share phenotypes with PRUNE1: view

Animal Models for PRUNE1 Gene

MGI Knock Outs for PRUNE1:

Animal Model Products

miRNA for PRUNE1 Gene

miRTarBase miRNAs that target PRUNE1

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PRUNE1 Gene

Localization for PRUNE1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRUNE1 Gene

Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PRUNE1 gene
Compartment Confidence
nucleus 5
cytosol 5
cytoskeleton 1

Gene Ontology (GO) - Cellular Components for PRUNE1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol IDA --
GO:0005925 focal adhesion IEA --
GO:0030054 cell junction IEA --
genes like me logo Genes that share ontologies with PRUNE1: view

Pathways & Interactions for PRUNE1 Gene

SuperPathways for PRUNE1 Gene

SuperPathway Contained pathways
1 Purine metabolism (KEGG)
genes like me logo Genes that share pathways with PRUNE1: view

Pathways by source for PRUNE1 Gene

1 KEGG pathway for PRUNE1 Gene

Gene Ontology (GO) - Biological Process for PRUNE1 Gene

None

No data available for SIGNOR curated interactions for PRUNE1 Gene

Drugs & Compounds for PRUNE1 Gene

(5) Drugs for PRUNE1 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
Manganese Approved Nutra 34
cyclic amp Experimental Pharma 0
Magnesium Nutra 0

(4) Additional Compounds for PRUNE1 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Guanosine 3',5'-bis(diphosphate)
Guanosine 3'-diphosphate 5'-triphosphate
Phosphate
  • NFB Orthophosphate
  • O-Phosphoric acid
  • Ortho-phosphate
  • Orthophosphate (PO43-)
  • Orthophosphate(3-)
14265-44-2
pyrophosphate
  • (4-)Diphosphoric acid ion
  • (P2O74-)Diphosphate
  • Diphosphate
  • Diphosphoric acid
  • PPi
14000-31-8
genes like me logo Genes that share compounds with PRUNE1: view

Transcripts for PRUNE1 Gene

Alternative Splicing Database (ASD) splice patterns (SP) for PRUNE1 Gene

No ASD Table

Relevant External Links for PRUNE1 Gene

GeneLoc Exon Structure for
PRUNE1
ECgene alternative splicing isoforms for
PRUNE1

Expression for PRUNE1 Gene

NURSA nuclear receptor signaling pathways regulating expression of PRUNE1 Gene:

PRUNE1

mRNA Expression by UniProt/SwissProt for PRUNE1 Gene:

Q86TP1-PRUNE_HUMAN
Tissue specificity: Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.

Evidence on tissue expression from TISSUES for PRUNE1 Gene

  • Nervous system(4.7)
  • Kidney(4.3)
  • Thyroid gland(4.3)
  • Blood(4.2)

Primer Products

No data available for mRNA expression in normal human tissues , mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for PRUNE1 Gene

Orthologs for PRUNE1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PRUNE1 Gene

Organism Taxonomy Gene Similarity Type Details
dog
(Canis familiaris)
Mammalia PRUNE 34 35
  • 91.02 (n)
cow
(Bos Taurus)
Mammalia PRUNE 34 35
  • 89.77 (n)
mouse
(Mus musculus)
Mammalia Prune 34 35
  • 85.28 (n)
Prune1 16
rat
(Rattus norvegicus)
Mammalia Prune 34
  • 84.92 (n)
oppossum
(Monodelphis domestica)
Mammalia PRUNE 35
  • 70 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PRUNE 35
  • 37 (a)
OneToOne
chicken
(Gallus gallus)
Aves PRUNE 34 35
  • 58.74 (n)
lizard
(Anolis carolinensis)
Reptilia PRUNE 35
  • 54 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia prune 34
  • 58.15 (n)
Str.16711 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.14865 34
zebrafish
(Danio rerio)
Actinopterygii LOC563343 34
  • 57.87 (n)
prune 35
  • 49 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta pn 34 35
  • 48.4 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP004314 34
  • 44.89 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes PPX1 34 35 37
  • 43 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ABL083W 34
  • 41.57 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B03806g 34
  • 41.03 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPAC2F3.11 34
  • 39.74 (n)
bread mold
(Neurospora crassa)
Ascomycetes NCU06310 34
  • 38.38 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 28 (a)
OneToOne
Species where no ortholog for PRUNE1 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • chimpanzee (Pan troglodytes)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for PRUNE1 Gene

ENSEMBL:
Gene Tree for PRUNE1 (if available)
TreeFam:
Gene Tree for PRUNE1 (if available)

Paralogs for PRUNE1 Gene

Pseudogenes.org Pseudogenes for PRUNE1 Gene

genes like me logo Genes that share paralogs with PRUNE1: view

No data available for Paralogs for PRUNE1 Gene

Variants for PRUNE1 Gene

Sequence variations from dbSNP and Humsavar for PRUNE1 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs886039608 Likely pathogenic 151,025,513(+) TCCAC(A/G)GGAAC intron-variant, splice-acceptor-variant
rs1000135973 -- 151,033,110(+) GACGG(A/G)GTTTC intron-variant
rs1000185357 -- 151,036,047(+) TTGCT(C/T)TTTTT downstream-variant-500B, upstream-variant-2KB
rs1000412972 -- 151,021,388(+) AGCCA(A/G)AGGTT intron-variant
rs1000472043 -- 151,012,052(+) GATTA(C/T)AGGCG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PRUNE1 Gene

Variant ID Type Subtype PubMed ID
nsv1127030 CNV deletion 24896259
nsv522840 CNV gain 19592680

Variation tolerance for PRUNE1 Gene

Residual Variation Intolerance Score: 61.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.76; 73.45% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PRUNE1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PRUNE1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PRUNE1 Gene

Disorders for PRUNE1 Gene

Relevant External Links for PRUNE1

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PRUNE1

No disorders were found for PRUNE1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PRUNE1 Gene

Publications for PRUNE1 Gene

  1. Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity. (PMID: 11687967) Forus A. … Zollo M. (Oncogene 2001) 2 3 4 64
  2. Evidence for interaction between human PRUNE and nm23-H1 NDPKinase. (PMID: 10602478) Reymond A. … Zollo M. (Oncogene 1999) 2 3 4 64
  3. Human metastasis regulator protein H-prune is a short-chain exopolyphosphatase. (PMID: 18700747) Tammenkoski M. … Lahti R. (Biochemistry 2008) 2 3 64
  4. Domain mapping on the human metastasis regulator protein h-Prune reveals a C-terminal dimerization domain. (PMID: 17655525) Middelhaufe S. … Steegborn C. (Biochem. J. 2007) 3 4 64
  5. Glycogen synthase kinase 3 and h-prune regulate cell migration by modulating focal adhesions. (PMID: 16428445) Kobayashi T. … Kikuchi A. (Mol. Cell. Biol. 2006) 3 4 64

Products for PRUNE1 Gene

Sources for PRUNE1 Gene

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