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Aliases for PRUNE Gene

Aliases for PRUNE Gene

  • Prune Exopolyphosphatase 2 3
  • Drosophila-Related Expressed Sequence 17 3 4
  • DRES-17 3 4
  • HTCD37 3 4
  • DRES17 3 4
  • Prune Homolog (Drosophila) 2
  • Protein Prune Homolog 3
  • EC 3.6.1.1 4
  • KIAA0367 6
  • PRUNE2 6
  • HPrune 4
  • BMCC1 6

External Ids for PRUNE Gene

Previous GeneCards Identifiers for PRUNE Gene

  • GC01P147796
  • GC01P149247
  • GC01P122357

Summaries for PRUNE Gene

Entrez Gene Summary for PRUNE Gene

  • This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

GeneCards Summary for PRUNE Gene

PRUNE (Prune Exopolyphosphatase) is a Protein Coding gene. Among its related pathways are Purine metabolism (KEGG). GO annotations related to this gene include manganese ion binding and inorganic diphosphatase activity.

UniProtKB/Swiss-Prot for PRUNE Gene

  • Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRUNE Gene

Genomics for PRUNE Gene

Regulatory Elements for PRUNE Gene

Genomic Location for PRUNE Gene

Start:
151,008,391 bp from pter
End:
151,035,713 bp from pter
Size:
27,323 bases
Orientation:
Plus strand

Genomic View for PRUNE Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PRUNE Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRUNE Gene

Proteins for PRUNE Gene

  • Protein details for PRUNE Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q86TP1-PRUNE_HUMAN
    Recommended name:
    Protein prune homolog
    Protein Accession:
    Q86TP1
    Secondary Accessions:
    • B2RCH8
    • B4DFL7
    • Q5SZF9
    • Q659E5
    • Q6P4E0
    • Q8N654
    • Q96JU5
    • Q9C071
    • Q9C072
    • Q9UIV0

    Protein attributes for PRUNE Gene

    Size:
    453 amino acids
    Molecular mass:
    50200 Da
    Cofactor:
    Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 manganese ions per subunit.;
    Quaternary structure:
    • Homooligomer. Able to homodimerize via its C-terminal domain. Interacts with NME1. Interacts with GSK3; at focal adhesion complexes where paxillin and vinculin are colocalized.
    SequenceCaution:
    • Sequence=BAB55423.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAG60534.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for PRUNE Gene

neXtProt entry for PRUNE Gene

Proteomics data for PRUNE Gene at MOPED

Post-translational modifications for PRUNE Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for PRUNE Gene

Domains for PRUNE Gene

Protein Domains for PRUNE Gene

Suggested Antigen Peptide Sequences for PRUNE Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q86TP1

UniProtKB/Swiss-Prot:

PRUNE_HUMAN :
  • Q86TP1
Family:
  • Belongs to the PPase class C family. Prune subfamily.
genes like me logo Genes that share domains with PRUNE: view

No data available for Gene Families for PRUNE Gene

Function for PRUNE Gene

Molecular function for PRUNE Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=0.91 uM for cAMP {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525}; KM=2.3 uM for cGMP {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525}; Vmax=12.8 pmol/min/ug enzyme with cAMP as substrate {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525}; Vmax=0.8 pmol/min/ug enzyme with cGMP as substrate {ECO:0000269 PubMed:14998490, ECO:0000269 PubMed:17655525};
UniProtKB/Swiss-Prot CatalyticActivity: Diphosphate + H(2)O = 2 phosphate
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by magnesium ions and inhibited by manganese ions. Inhibited by dipyridamole, moderately sensitive to IBMX and inhibited by vinpocetine.
UniProtKB/Swiss-Prot Function: Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.

Enzyme Numbers (IUBMB) for PRUNE Gene

Gene Ontology (GO) - Molecular Function for PRUNE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004427 inorganic diphosphatase activity IEA --
GO:0005515 protein binding IPI 10602478
GO:0016462 pyrophosphatase activity --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with PRUNE: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PRUNE

In Situ Assay Products

Flow Cytometry Products

No data available for Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for PRUNE Gene

Localization for PRUNE Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRUNE Gene

Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PRUNE Gene COMPARTMENTS Subcellular localization image for PRUNE gene
Compartment Confidence
nucleus 5
cytosol 3
cytoskeleton 1

Gene Ontology (GO) - Cellular Components for PRUNE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IDA --
GO:0005925 focal adhesion IEA --
GO:0030054 cell junction --
genes like me logo Genes that share ontologies with PRUNE: view

Pathways for PRUNE Gene

SuperPathways for PRUNE Gene

Superpath Contained pathways
1 Purine metabolism (KEGG)
genes like me logo Genes that share pathways with PRUNE: view

Pathways by source for PRUNE Gene

1 KEGG pathway for PRUNE Gene

Gene Ontology (GO) - Biological Process for PRUNE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008152 metabolic process IEA --
genes like me logo Genes that share ontologies with PRUNE: view

Compounds for PRUNE Gene

(5) HMDB Compounds for PRUNE Gene

Compound Synonyms Cas Number PubMed IDs
Cyclic AMP
  • Cyclic AMP
60-92-4
Magnesium
  • Magnesium
7439-95-4
Manganese
  • manganese
7439-96-5
Phosphate
  • NFB Orthophosphate
14265-44-2
Water
  • Dihydrogen oxide
7732-18-5
genes like me logo Genes that share compounds with PRUNE: view

Transcripts for PRUNE Gene

Unigene Clusters for PRUNE Gene

Prune homolog (Drosophila):
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PRUNE

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PRUNE Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2a · 2b ^ 3a · 3b · 3c ^ 4a · 4b · 4c ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c · 9d · 9e
SP1: - -
SP2: - - - - -
SP3: - - - - - - -
SP4: -
SP5: - - -
SP6: - - - - - - - - - -
SP7: - - - - - - -
SP8: - -
SP9: - -
SP10: -
SP11: - - - - - - - - - - - - -
SP12:

Relevant External Links for PRUNE Gene

GeneLoc Exon Structure for
PRUNE
ECgene alternative splicing isoforms for
PRUNE

Expression for PRUNE Gene

mRNA expression in normal human tissues for PRUNE Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for PRUNE Gene

SOURCE GeneReport for Unigene cluster for PRUNE Gene Hs.78524

mRNA Expression by UniProt/SwissProt for PRUNE Gene

Q86TP1-PRUNE_HUMAN
Tissue specificity: Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
genes like me logo Genes that share expressions with PRUNE: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for PRUNE Gene

Orthologs for PRUNE Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PRUNE Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia PRUNE 35
  • 89.77 (n)
  • 88.52 (a)
PRUNE 36
  • 89 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PRUNE 35
  • 91.02 (n)
  • 90.51 (a)
PRUNE 36
  • 91 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Prune 35
  • 85.28 (n)
  • 85.65 (a)
Prune 16
Prune 36
  • 85 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia PRUNE 36
  • 70 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PRUNE 36
  • 37 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Prune 35
  • 84.92 (n)
  • 85.43 (a)
chicken
(Gallus gallus)
Aves LOC100857290 35
  • 58.74 (n)
  • 57.28 (a)
PRUNE 36
  • 56 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PRUNE 36
  • 54 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.14865 35
tropical clawed frog
(Silurana tropicalis)
Amphibia prune 35
  • 58.15 (n)
  • 53.11 (a)
Str.16711 35
zebrafish
(Danio rerio)
Actinopterygii LOC563343 35
  • 57.87 (n)
  • 50.69 (a)
prune 36
  • 49 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP004314 35
  • 44.89 (n)
  • 35.24 (a)
fruit fly
(Drosophila melanogaster)
Insecta pn 35
  • 48.4 (n)
  • 37.13 (a)
pn 36
  • 31 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ABL083W 35
  • 41.57 (n)
  • 27.35 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes PPX1 35
  • 43 (n)
  • 30.51 (a)
PPX1 36
  • 24 (a)
OneToOne
PPX1 38
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B03806g 35
  • 41.03 (n)
  • 29.29 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU06310 35
  • 38.38 (n)
  • 28.01 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPAC2F3.11 35
  • 39.74 (n)
  • 27.93 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 28 (a)
OneToOne
Species with no ortholog for PRUNE:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • chimpanzee (Pan troglodytes)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for PRUNE Gene

ENSEMBL:
Gene Tree for PRUNE (if available)
TreeFam:
Gene Tree for PRUNE (if available)

Paralogs for PRUNE Gene

Selected SIMAP similar genes for PRUNE Gene using alignment to 4 proteins:

Pseudogenes.org Pseudogenes for PRUNE Gene

genes like me logo Genes that share paralogs with PRUNE: view

No data available for Paralogs for PRUNE Gene

Variants for PRUNE Gene

Sequence variations from dbSNP and Humsavar for PRUNE Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs1673161 -- 151,014,186(+) aggtt(A/G)gccag intron-variant
rs2445894 -- 151,014,754(+) actgg(G/T)ggcag intron-variant
rs2497992 -- 151,012,106(-) TTTTT(C/T)TTTTT intron-variant
rs2864701 -- 151,028,386(-) ggcac(A/G)cacca intron-variant
rs2864702 -- 151,027,783(-) CGCGC(A/G)CACAC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PRUNE Gene

Variant ID Type Subtype PubMed ID
nsv522840 CNV Gain 19592680

Relevant External Links for PRUNE Gene

HapMap Linkage Disequilibrium report
PRUNE

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PRUNE Gene

Disorders for PRUNE Gene

Relevant External Links for PRUNE

Genetic Association Database (GAD)
PRUNE
Human Genome Epidemiology (HuGE) Navigator
PRUNE
genes like me logo Genes that share disorders with PRUNE: view

No data available for UniProtKB/Swiss-Prot for PRUNE Gene

Publications for PRUNE Gene

  1. Evidence for interaction between human PRUNE and nm23-H1 NDPKinase. (PMID: 10602478) Reymond A. … Zollo M. (Oncogene 1999) 2 3 4
  2. Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity. (PMID: 11687967) Forus A. … Zollo M. (Oncogene 2001) 2 3 4
  3. Sequencing analysis of forty-eight human image cDNA clones similar to Drosophila mutant protein. (PMID: 10524757) Volorio S. … Zollo M. (DNA Seq. 1998) 3 4
  4. Overexpression of h-prune in breast cancer is correlated with advanced disease status. (PMID: 15671547) Zollo M. … Palmieri G. (Clin. Cancer Res. 2005) 3 4
  5. Glycogen synthase kinase 3 and h-prune regulate cell migration by modulating focal adhesions. (PMID: 16428445) Kobayashi T. … Kikuchi A. (Mol. Cell. Biol. 2006) 3 4

Products for PRUNE Gene

Sources for PRUNE Gene

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