Free for academic non-profit institutions. Other users need a Commercial license

Aliases for PRSS21 Gene

Aliases for PRSS21 Gene

  • Protease, Serine, 21 (Testisin) 2 3
  • ESP1 3 4 6
  • Eosinophil Serine Protease 1 3 4
  • ESP-1 3 4
  • TEST1 3 4
  • Serine Protease From Eosinophils 3
  • Serine Protease 21 4
  • EC 3.4.21.- 4
  • EC 3.4.21 63

External Ids for PRSS21 Gene

Previous GeneCards Identifiers for PRSS21 Gene

  • GC16P002887
  • GC16P002867
  • GC16P002807
  • GC16P002833

Summaries for PRSS21 Gene

Entrez Gene Summary for PRSS21 Gene

  • This gene encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. The encoded protein is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The encoded protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and may be involved in progression of testicular tumors of germ cell origin. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

GeneCards Summary for PRSS21 Gene

PRSS21 (Protease, Serine, 21 (Testisin)) is a Protein Coding gene. Diseases associated with PRSS21 include dracunculiasis and testicular cancer. GO annotations related to this gene include serine-type endopeptidase activity and serine-type peptidase activity. An important paralog of this gene is TPSAB1.

UniProtKB/Swiss-Prot for PRSS21 Gene

  • Could regulate proteolytic events associated with testicular germ cell maturation

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRSS21 Gene

Genomics for PRSS21 Gene

Regulatory Elements for PRSS21 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for PRSS21 Gene

2,817,163 bp from pter
2,826,304 bp from pter
9,142 bases
Plus strand

Genomic View for PRSS21 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PRSS21 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRSS21 Gene

Proteins for PRSS21 Gene

  • Protein details for PRSS21 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • Q9NS34
    • Q9P2V6

    Protein attributes for PRSS21 Gene

    314 amino acids
    Molecular mass:
    34884 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for PRSS21 Gene


neXtProt entry for PRSS21 Gene

Proteomics data for PRSS21 Gene at MOPED

Selected DME Specific Peptides for PRSS21 Gene

Post-translational modifications for PRSS21 Gene

  • Glycosylation at Asn167, Asn200, and Asn273
  • Modification sites at PhosphoSitePlus

Domains for PRSS21 Gene

Gene Families for PRSS21 Gene

  • PRSS :Serine peptidases / Serine peptidases

Suggested Antigen Peptide Sequences for PRSS21 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Q9Y6M0
  • Contains 1 peptidase S1 domain.
  • Belongs to the peptidase S1 family.
genes like me logo Genes that share domains with PRSS21: view

Function for PRSS21 Gene

Molecular function for PRSS21 Gene

GENATLAS Biochemistry: protease serine 21,testisin,abundantly expressed in the testis and in testicular tumors,see TSG16D (role in progression of testicular tumor of germ cell origin)
UniProtKB/Swiss-Prot Function: Could regulate proteolytic events associated with testicular germ cell maturation

Enzyme Numbers (IUBMB) for PRSS21 Gene

Gene Ontology (GO) - Molecular Function for PRSS21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003824 catalytic activity --
GO:0004252 serine-type endopeptidase activity IEA --
GO:0005515 protein binding IPI 20211623
GO:0008236 serine-type peptidase activity TAS 10397266
genes like me logo Genes that share ontologies with PRSS21: view

Phenotypes for PRSS21 Gene

MGI mutant phenotypes for PRSS21:
inferred from 2 alleles
GenomeRNAi human phenotypes for PRSS21:
genes like me logo Genes that share phenotypes with PRSS21: view

Animal Models for PRSS21 Gene

MGI Knock Outs for PRSS21:

Animal Model Products

CRISPR Products

miRNA for PRSS21 Gene

miRTarBase miRNAs that target PRSS21

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PRSS21

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for PRSS21 Gene

Localization for PRSS21 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRSS21 Gene

Cell membrane; Lipid-anchor, GPI-anchor.

Subcellular locations from

Jensen Localization Image for PRSS21 Gene COMPARTMENTS Subcellular localization image for PRSS21 gene
Compartment Confidence
plasma membrane 5
cytosol 3
extracellular 3
lysosome 1
nucleus 1

Gene Ontology (GO) - Cellular Components for PRSS21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm TAS 10397266
GO:0005886 plasma membrane IEA --
GO:0016020 membrane TAS 9826525
GO:0031225 anchored component of membrane IEA --
genes like me logo Genes that share ontologies with PRSS21: view

Pathways for PRSS21 Gene

SuperPathways for PRSS21 Gene

No Data Available

Gene Ontology (GO) - Biological Process for PRSS21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0007283 spermatogenesis IEA --
genes like me logo Genes that share ontologies with PRSS21: view

No data available for Pathways by source for PRSS21 Gene

Compounds for PRSS21 Gene

(1) Novoseek inferred chemical compound relationships for PRSS21 Gene

Compound -log(P) Hits PubMed IDs
serine 68.7 18
genes like me logo Genes that share compounds with PRSS21: view

Transcripts for PRSS21 Gene

Unigene Clusters for PRSS21 Gene

Protease, serine, 21 (testisin):
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PRSS21

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PRSS21 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3a · 3b · 3c · 3d ^ 4a · 4b · 4c · 4d
SP1: - -
SP2: -
SP3: - - -
SP4: -
SP5: -

Relevant External Links for PRSS21 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for PRSS21 Gene

mRNA expression in normal human tissues for PRSS21 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PRSS21 Gene

This gene is overexpressed in Testis (34.4).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for PRSS21 Gene

SOURCE GeneReport for Unigene cluster for PRSS21 Gene Hs.72026

mRNA Expression by UniProt/SwissProt for PRSS21 Gene

Tissue specificity: Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes
genes like me logo Genes that share expressions with PRSS21: view

In Situ Assay Products

Orthologs for PRSS21 Gene

This gene was present in the common ancestor of animals.

Orthologs for PRSS21 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia PRSS21 35
  • 99.28 (n)
  • 99.28 (a)
PRSS21 36
  • 99 (a)
(Bos Taurus)
Mammalia PRSS21 35
  • 74.43 (n)
  • 65.95 (a)
PRSS21 36
  • 64 (a)
(Canis familiaris)
Mammalia -- 36
  • 25 (a)
PRSS21 35
  • 76.21 (n)
  • 67.56 (a)
(Mus musculus)
Mammalia Prss21 35
  • 72.72 (n)
  • 68.84 (a)
Prss21 16
Prss21 36
  • 63 (a)
(Monodelphis domestica)
Mammalia -- 36
  • 35 (a)
-- 36
  • 38 (a)
-- 36
  • 40 (a)
(Rattus norvegicus)
Mammalia Prss21 35
  • 73.45 (n)
  • 69.12 (a)
(Anolis carolinensis)
Reptilia -- 36
  • 38 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG8170 37
  • 37 (a)
CG13744 37
  • 31 (a)
CG11824 37
  • 38 (a)
(Caenorhabditis elegans)
Secernentea ZK546.15 37
  • 33 (a)
Species with no ortholog for PRSS21:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for PRSS21 Gene

Gene Tree for PRSS21 (if available)
Gene Tree for PRSS21 (if available)

Paralogs for PRSS21 Gene Pseudogenes for PRSS21 Gene

genes like me logo Genes that share paralogs with PRSS21: view

Variants for PRSS21 Gene

Sequence variations from dbSNP and Humsavar for PRSS21 Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type MAF
rs10641 -- 2,821,554(+) TGGCC(A/G)CTACT utr-variant-3-prime, synonymous-codon, reference, nc-transcript-variant
rs2072271 -- 2,821,752(-) CTGCA(C/T)AAGAG downstream-variant-500B
rs2072272 -- 2,821,722(-) GCCAC(C/T)GCTTT utr-variant-3-prime, nc-transcript-variant, downstream-variant-500B
rs2072273 - 2,821,451(-) TGGGC(C/T)GACCA utr-variant-3-prime, missense, reference, nc-transcript-variant
rs2072274 -- 2,820,878(-) CGGGG(A/G)TGGGG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PRSS21 Gene

Variant ID Type Subtype PubMed ID
nsv905152 CNV Loss 21882294
dgv793e1 CNV Complex 17122850
nsv905162 CNV Gain 21882294
nsv905165 CNV Loss 21882294
dgv2573n71 CNV Loss 21882294
nsv905169 CNV Loss 21882294
nsv905170 CNV Loss 21882294
nsv905173 CNV Loss 21882294

Relevant External Links for PRSS21 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PRSS21 Gene

Disorders for PRSS21 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (3) Diseases for PRSS21 Gene including...

Search for PRSS21 Gene in MalaCards »

(1) University of Copenhagen DISEASES for PRSS21 Gene

(4) Novoseek inferred disease relationships for PRSS21 Gene

Disease -log(P) Hits PubMed IDs
testicular cancer 59.4 3
ovarian carcinoma 49.8 3
ovarian cancer 24.4 3
tumors 16.5 9
genes like me logo Genes that share disorders with PRSS21: view

No data available for UniProtKB/Swiss-Prot for PRSS21 Gene

Publications for PRSS21 Gene

  1. Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors. (PMID: 10397266) Hooper J.D. … Antalis T.M. (Cancer Res. 1999) 2 3 4 23
  2. Localization, expression and genomic structure of the gene encoding the human serine protease testisin. (PMID: 11004480) Hooper J.D. … Antalis T.M. (Biochim. Biophys. Acta 2000) 3 4 23
  3. Cloning and tissue distribution of a novel serine protease esp-1 from human eosinophils. (PMID: 9826525) Inoue M. … Kido H. (Biochem. Biophys. Res. Commun. 1998) 2 3 4
  4. Structural analysis of esp-1 gene (PRSS 21). (PMID: 10600542) Inoue M. … Kido H. (Biochem. Biophys. Res. Commun. 1999) 3 4
  5. Epigenetic silencing of the putative tumor suppressor gene testisin in testicular germ cell tumors. (PMID: 16810501) Kempkensteffen C. … Schrader M. (J. Cancer Res. Clin. Oncol. 2006) 3 23

Products for PRSS21 Gene

Sources for PRSS21 Gene

Back to Top