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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

PRMT5 Gene

protein-coding   GIFtS: 62
GCID: GC14M023389

protein arginine methyltransferase 5

(Previous names: skb1 (S. pombe) homolog, SKB1 homolog (S. pombe) )
(Previous symbols: HRMT1L5, SKB1)
 Explore 9 diseases affiliated with
PRMT5 via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for PRMT5    

Aliases
for PRMT5 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
Protein Arginine Methyltransferase 51 2     JBP12 3
HRMT1L51 2 3     SKB1 Homolog2 3
SKB11 2 3     Skb1 (S. Pombe) Homolog1
SKB1Hs1 2 3     SKB1 Homolog (S. Pombe)1
Histone-Arginine N-Methyltransferase PRMT52 3     HMT1 HnRNP Methyltransferase-Like 52
Jak-Binding Protein 12 3     Protein Arginine N-Methyltransferase 52
Shk1 Kinase-Binding Protein 1 Homolog2 3     EC 2.1.1.-3
72 KDa ICln-Binding Protein2 3     EC 2.1.1.1253
IBP722 3     

External Ids:    HGNC: 108941   Entrez Gene: 104192   Ensembl: ENSG000001004627   OMIM: 6040455   UniProtKB: O147443   

Export aliases for PRMT5 gene to outside databases

Previous GC identifers: GC14M022460 GC14M003506


Summaries
for PRMT5 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
UniProtKB/Swiss-Prot: ANM5_HUMAN, O14744
Function: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and
symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical
dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such
methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and
dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core
particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter
activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a
pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A
and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.
Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the
interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances
EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second,
methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic
activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation.
Methylates HOXA9. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation

Gene Wiki entry for PRMT5 (Protein arginine methyltransferase 5)


Genomic Views
for PRMT5 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000014.8  NC_018925.1  NT_026437.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the PRMT5 gene promoter:
         HOXA9B   HOXA9   Tal-1   MyoD   POU3F2 (N-Oct-5a)   POU3F2 (N-Oct-5b)   Meis-1b   ATF   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: PRMT5 promoter sequence
   Search SABiosciences Chromatin IP Primers for PRMT5

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PRMT5


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q11.2   Ensembl cytogenetic band:  14q11.2   HGNC cytogenetic band: 14q11.2

PRMT5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PRMT5 gene location

GeneLoc information about chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M023389:  view genomic region     (about GC identifiers)

Start:
 23,389,720 bp from pter      End:
 23,398,794 bp from pter
Size:
 9,075 bases      Orientation:
 minus strand

Proteins
for PRMT5 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: ANM5_HUMAN, O14744 (See protein sequence)
Recommended Name: Protein arginine N-methyltransferase 5  
Size: 637 amino acids; 72684 Da
Subunit: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the
methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight
E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and
SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4. Interacts with LSM11, PRMT7 and
SNRPD3. Interacts with COPRS; promoting its recruitment on histone H4. Interacts with RPS10. Interacts with EGFR;
methylates EGFR and stimulates EGFR-mediated ERK activation. Interacts with BRAF and with active RAF1. Interacts with
HOXA9. Interacts with SRGAP2. Found in a complex with COPRS, RUNX1 AND CBFB. Interacts with CHTOP; the interaction
symmetrically methylates CHTOP, but seems to require the presence of PRMT1 (By similarity)
Subcellular location: Cytoplasm. Nucleus
1 PDB 3D structure from and Proteopedia for PRMT5:
4GQB (3D)    
Secondary accessions: A8MZ91 B5BU10 D3DS33 Q6IBR1 Q9UKH1
Alternative splicing: 2 isoforms:  O14744-1   O14744-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for PRMT5: NX_O14744

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O14744

    • PRMT5 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001034708.1  NP_006100.2  

    ENSEMBL proteins: 
     ENSP00000319169   ENSP00000380583   ENSP00000452501   ENSP00000380582   ENSP00000216350  
     ENSP00000451245   ENSP00000387663   ENSP00000451127   ENSP00000452555   ENSP00000450956  
     ENSP00000452409   ENSP00000452509   ENSP00000450737   ENSP00000452102   ENSP00000452218  
     ENSP00000450633   ENSP00000450919   ENSP00000452411   ENSP00000409482   ENSP00000444915  
    Reactome Protein details: O14744
    Human Recombinant Protein Products: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein: PRMT5
    OriGene Protein Over-expression Lysate: PRMT5
    OriGene Custom Protein Services for PRMT5 
    GenScript Custom Purified and Recombinant Proteins Services for PRMT5
    Novus Biologicals PRMT5 Proteins
    Novus Biologicals PRMT5 Lysates
    Sino Biological Recombinant Protein for PRMT5
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for PRMT5

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus NAS18404153
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0043234protein complex IEA--


    PRMT5 for ontologies           About GeneDecksing



    PRMT5 Antibody Products: 
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    Abcam antibodies for PRMT5 
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    ThermoFisher Antibodies for PRMT5

    Assay Products for PRMT5: 
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    Uscn ELISAs and CLIAs for PRMT5

    Protein Domains /
    Families
    for PRMT5 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    PRMT5 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR007857 Arg_MeTrfase_PRMT5
     IPR025799 Arg_MeTrfase

    Graphical View of Domain Structure for InterPro Entry O14744

    ProtoNet protein and cluster: O14744

    1 Blocks protein family: IPB007857 Skb1 methyltransferase

    UniProtKB/Swiss-Prot: ANM5_HUMAN, O14744
    Similarity: Belongs to the protein arginine N-methyltransferase family


    Function
    for PRMT5 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: ANM5_HUMAN, O14744
    Function: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and
    symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical
    dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such
    methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and
    dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core
    particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter
    activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a
    pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A
    and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.
    Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the
    interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
    Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances
    EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second,
    methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic
    activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation.
    Methylates HOXA9. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation
    Catalytic activity: S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine +
    N(omega)-methyl-arginine-[histone]
    Enzyme regulation: Activity is increased by EGF, HGF, FGF1 or FGF2 treatments, and slightly decreased by NGF treatment

    Enzyme Numbers (IUBMB): EC 2.1.1.-1 EC 2.1.1.1251

    miRNA
    Products:            		miRTarBase miRNAs that target PRMT5:
    hsa-mir-92b (MIRT004351), hsa-mir-25 (MIRT000983), hsa-mir-96 (MIRT004352), hsa-mir-32 (MIRT000982), hsa-mir-19a (MIRT004350)

    OriGene 3'-UTR Clone (see all 2): PRMT5
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat PRMT5
    5 QIAGEN miScript miRNA Assays for microRNAs that regulate PRMT5:
    hsa-miR-3149 hsa-miR-1233 hsa-miR-1266 hsa-miR-2115 hsa-miR-3647-3p
    SwitchGear 3'UTR luciferase reporter plasmid: PRMT5 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PRMT5 (see all 7)
    OriGene shRNA RFP: PRMT5
    OriGene siRNA: PRMT5
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PRMT5

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for PRMT5

    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PRMT5 (see all 4)
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    GenScript: all cDNA clones in your preferred vector (see all 2): PRMT5 (NM_006109)
    Sino Biological Human cDNA Clone for PRMT5
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRMT5 

    Cell Line
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    Search LifeMap BioReagents cell lines for PRMT5

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRMT5

    Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003682chromatin binding IEA--
    GO:0005515protein binding IPI--
    GO:0008168methyltransferase activity IDA15369763
    GO:0008469histone-arginine N-methyltransferase activity IEA--
    GO:0035243protein-arginine omega-N symmetric methyltransferase activity IMP17709427


    PRMT5 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for PRMT5:
     Increased G1 DNA content 

    Animal Models:
         3 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Prmt5):
     cellular  embryogenesis  mortality/aging 

    PRMT5 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for PRMT5 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/8 super-pathways (see all 8) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1SLBP independent Processing of Histone Pre-mRNAs
    		snRNP Assembly0.28
    		Metabolism of non-coding RNA0.28
    2p53 pathway
    		p53 pathway1.00
    3Signaling events mediated by HDAC Class I
    		Signaling events mediated by HDAC Class I1.00
    4E2F transcription factor network
    		E2F transcription factor network1.00
    5RNA transport
    		RNA transport1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for PRMT5
        Chromatin Regulation / Acetylation

    3 BioSystems Pathways for PRMT5 
        p53 pathway
    E2F transcription factor network
    Signaling events mediated by HDAC Class I

    4        Reactome Pathways for PRMT5
        snRNP Assembly
    Metabolism of RNA
    Gene Expression
    Metabolism of non-coding RNA


    1         Kegg Pathway  (Kegg details for PRMT5):
        RNA transport


    PRMT5 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for PRMT5

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/360 Interacting proteins for PRMT5 (O147441, 2, 3 ENSP000003191694) via UniProtKB, MINT, STRING, and/or I2D (see all 360)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628051, 2, 3, ENSP000003529804EBI-351098,EBI-302023 MINT-56509 I2D: score=3 STRING: ENSP00000352980
    HIST1H4LP628051, 2, 3, ENSP000003482584EBI-351098,EBI-302023 MINT-56509 I2D: score=3 STRING: ENSP00000348258
    HIST1H4BP628051, 2, 3EBI-351098,EBI-302023 MINT-56509 I2D: score=3 
    HIST1H4CP628051, 2, 3EBI-351098,EBI-302023 MINT-56509 I2D: score=3 
    HIST1H4DP628051, 2, 3EBI-351098,EBI-302023 MINT-56509 I2D: score=3 
    About this table

    Gene Ontology (GO): 5/14 biological process terms (GO ID links to tree view) (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000122negative regulation of transcription from RNA polymerase II promoter IEA--
    GO:0000387spliceosomal snRNP assembly TAS--
    GO:0006351transcription, DNA-dependent IEA--
    GO:0006479protein methylation ----
    GO:0007088regulation of mitosis TAS9843966


    PRMT5 for ontologies           About GeneDecksing



    Drugs & Compounds
    for PRMT5 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    PRMT5 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for PRMT5

    4 HMDB Compounds for PRMT5    About this table
    CompoundSynonyms CAS #PubMed Ids
    ChlorineCl2 (see all 13)16887-00-6--
    Cob(II)alaminVitamin B12r (see all 5)14463-33-3--
    S-Adenosylhomocysteine(S)-5'-(S)-(3-Amino-3-carboxypropyl)-5'-thioadenosine (see all 19)979-92-0--
    S-Adenosylmethionine(3S)-5'-[(3-amino-3-carboxypropyl)methylsulfonio]-5'-deoxyadenosine (see all 16)29908-03-0--
    2 Novoseek chemical compound relationships for PRMT5 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    arginine 72.6 46 17437848 (7), 17974993 (3), 11413150 (2), 19086919 (2) (see all 20)
    testosterone 0.167 2 17437848 (2)

    Search CenterWatch for drugs/clinical trials and news about PRMT5 / ANM5 

    Transcripts
    for PRMT5 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for PRMT5 gene (2 alternative transcripts): 
    NM_001039619.1  NM_006109.3  

    Unigene Cluster for PRMT5:

    Protein arginine methyltransferase 5
    Hs.367854  [show with all ESTs]
    Unigene Representative Sequence: NM_001039619
    18/28 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 28):
    ENST00000324366(uc001whm.1 uc010tnh.1) ENST00000397441(uc001whl.1 uc010tnf.1 uc010tng.1 uc001whn.1)
    ENST00000557443 ENST00000476175 ENST00000397440 ENST00000216350 ENST00000555454
    ENST00000454731 ENST00000553915 ENST00000553897 ENST00000557758 ENST00000553502
    ENST00000555530 ENST00000556043 ENST00000553550 ENST00000554716 ENST00000553787
    ENST00000553641

    miRNA
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    5 QIAGEN miScript miRNA Assays for microRNAs that regulate PRMT5:
    hsa-miR-3149 hsa-miR-1233 hsa-miR-1266 hsa-miR-2115 hsa-miR-3647-3p
    SwitchGear 3'UTR luciferase reporter plasmid: PRMT5 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PRMT5 (see all 7)
    OriGene shRNA RFP: PRMT5
    OriGene siRNA: PRMT5
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PRMT5
    Clone
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    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector (see all 2): PRMT5 (NM_006109)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PRMT5
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRMT5 
    Primer
    Products:            		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PRMT5
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat PRMT5
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PRMT5
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PRMT5

    Additional cDNA sequence: 

    AB451246.1 AB451370.1 AF015913.1 AF167572.1 AK027737.1 AK075251.1 AK300863.1 AK301812.1 
    AK302240.1 AK315500.1 AL833612.1 BC005820.2 BC025979.2 CR456741.1 

    24/35 DOTS entries (see all 35):

    DT.443547  DT.101969883  DT.120777773  DT.100709939  DT.100681614  DT.100649367  DT.100681617  DT.120777844 
    DT.100681609  DT.100030872  DT.120777751  DT.100857670  DT.120777544  DT.120777507  DT.97855476  DT.95366575 
    DT.85100215  DT.95366561  DT.95366580  DT.95366583  DT.97855479  DT.100847465  DT.100658420  DT.100694208 

    24/575 AceView cDNA sequences (see all 575):

    AU104340 BQ005619 BU557366 CN481405 BM748444 CB157151 BX437760 BI831062 
    BM554644 BM757722 BM827947 BM835883 BG055754 BM827318 BE019057 BQ923839 
    CR625183 BX333330 BM559652 AW305304 BX388768 BM758698 BP339177 BQ901108 

    GeneLoc Exon Structure

    5/19 Alternative Splicing Database (ASD) splice patterns (SP) for PRMT5 (see all 19)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b · 11c ^ 12a · 12b ^
    SP1:                                      -     -                                                           -                                                   
    SP2:                                                                                                        -                                                   
    SP3:                                      -     -                                   -     -     -     -     -     -                                             
    SP4:                                      -     -                       -     -     -     -     -     -     -     -                                             
    SP5:                                      -     -     -                                                     -                                                   

    ExUns: 13a · 13b ^ 14a · 14b ^ 15a · 15b ^ 16 ^ 17a · 17b ^ 18 ^ 19a · 19b
    SP1:                                                                        
    SP2:                                                                        
    SP3:                                                                        
    SP4:                                                                        
    SP5:                                                                        


    ECgene alternative splicing isoforms for PRMT5

    Expression
    for PRMT5 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    PRMT5 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGGTTTGAAC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    PRMT5 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    Reproductive SystemEarly GonadPrimordial Germ CellsGerm Cells
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See PRMT5 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for PRMT5

    SOURCE GeneReport for Unigene cluster: Hs.367854

    UniProtKB/Swiss-Prot: ANM5_HUMAN, O14744
    Tissue specificity: Ubiquitous

        SABiosciences Expression via Pathway-Focused PCR Array including PRMT5: 
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    Orthologs
    for PRMT5 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of eukaryotes.

    Orthologs for PRMT5 gene from 8/33 species (see all 33)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    mouse
    (Mus musculus)    		 Mammalia Prmt51 , 5 protein arginine N-methyltransferase 51, 5 91.68(n)1
    97.96(a)1    		   14 (27.82 cM)5
    273741  NM_013768.31  NP_038796.21 
     545071875 
    lizard
    (Anolis carolinensis)    		 Reptilia PRMT56
    		 --
    		 84(a)
    		 1 ↔ 1
    		 GL343879.1(56532-75894)
    African clawed frog
    (Xenopus laevis)    		 Amphibia BX846887.12   -- 75.7(n)    BX846887.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii skb12 SKB1 homolog (S. pombe) 77.84(n)   368664  57044391 
    fruit fly
    (Drosophila melanogaster)    		 Insecta csul1 capsuleen 50.5(n)
    42.12(a)    		   36809  NM_057836.3  NP_477184.1 
    worm
    (Caenorhabditis elegans)    		 Secernentea prmt-56
    		 Protein arginine N-methyltransferase 5
    		 28(a)
    		 1 ↔ 1
    		 III(5230516-5234568)
    thale cress
    (Arabidopsis thaliana)    		 eudicotyledons SKB11 protein arginine N-methyltransferase 5 54.54(n)
    50.34(a)    		   829240  NM_119262.5  NP_194841.2 
    rice
    (Oryza sativa)    		 Liliopsida Os.177722 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 73.38(n)    AK103804.1 


    ENSEMBL Gene Tree for PRMT5 (if available)
    TreeFam Gene Tree for PRMT5 (if available) 

    Paralogs
    for PRMT5 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for PRMT5 gene

    PRMT5 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for PRMT5
    PGOHUM00000261903


    Genomic Variants
    for PRMT5 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/207 NCBI SNPs in PRMT5 are shown (see all 207)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 14 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs786178901,2
    		C,--3506511(+) NNNNAC/ATATTC 1 -- nc-transcript-variant2Minor allele frequency- A:0.04NA 122
    rs455367321,2
    		F,--3506828(+) AACAAG/CAACAG 1 -- nc-transcript-variant2Minor allele frequency- C:0.05WA NA 238
    rs1151511031,2
    		C,F,--3507644(+) CTGAGG/ATAGGA 1 -- int11Minor allele frequency- A:0.02WA 118
    rs71546181,2
    		C,F,--3507998(+) tggcaC/Tctgta 1 -- int11Minor allele frequency- T:0.03WA 118
    rs1996319201,2
    		C--3508566(+) AATCAA/CANNNN 1 -- int10--------
    rs783113941,2
    		--3508572(+) ACAAAC/GATCTC 1 -- int10--------
    rs789576211,2
    		C,--3508715(+) AGTCTA/CAAAGT 1 -- int10--------
    rs780106891,2
    		C,--3508786(+) GGTTGG/TTGTCA 1 -- int10--------
    rs116236801,2
    		C,H--3508822(+) TATGTG/CGGTGA 1 -- int14Minor allele frequency- C:0.00NS EA 416
    rs1128647911,2
    		C,F,--3509908(+) CGCCAT/CGCCTG 1 -- ds50013Minor allele frequency- C:0.40WA CSA 5

    HapMap Linkage Disequilibrium report for PRMT5 (23389720 - 23398794 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for PRMT5: --

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    Disorders / Diseases
    for PRMT5 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    PRMT5 for disorders           About GeneDecksing

    OMIM gene information: 604045    OMIM disorders: --

    9 diseases for PRMT5:    About MalaCards
    leukemia/lymphoma    mantle cell lymphoma    leukemia/lymphoma, t-cell    germ cell tumor
    homocysteine    ovarian cancer    gastric cancer    leukemia
    malaria

    1 Novoseek disease relationship for PRMT5 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    cancer 3.92 5 17627275 (2)


    Export disorders for PRMT5 gene to outside databases

    Publications
    for PRMT5 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for PRMT5 gene, integrated from 9 sources (see all 123):
    (articles sorted by number of sources associating them with PRMT5)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs. (PubMed id 9843966)1, 2, 3 Gilbreth M.... Marcus S. (1998)
    2. Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family. (PubMed id 11152681)1, 2, 9 Rho J.... Im D.-S. (2001)
    3. The histone-binding protein COPR5 is required for nuclear functions of the protein arginine methyltransferase PRMT5. (PubMed id 18404153)1, 2, 9 Lacroix M....Fabbrizio E. (2008)
    4. The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity. (PubMed id 10531356)1, 2, 9 Pollack B.P....Pestka S. (1999)
    5. Two distinct arginine methyltransferases are required for biogenesis of Sm-class ribonucleoproteins. (PubMed id 17709427)1, 2, 9 Gonsalvez G.B....Matera A.G. (2007)
    6. Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes. (PubMed id 16087681)1, 2, 9 Azzouz T.N.... Schuemperli D. (2005)
    7. Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties. (PubMed id 12718890)1, 2, 9 Kwak Y.T.... Gaynor R.B. (2003)
    8. Crystal structure of the human PRMT5:MEP50 complex. (PubMed id 23071334)1, 2 Antonysamy S....Emtage S. (2012)
    9. HOXA9 methylation by PRMT5 is essential for endothelial cell expression of leukocyte adhesion molecules. (PubMed id 22269951)1, 2 Bandyopadhyay S.... Dicorleto P.E. (2012)
    10. Protein arginine methyltransferase 5 regulates ERK1/2 signal transduction amplitude and cell fate through CRAF. (PubMed id 21917714)1, 2 Andreu-PAcrez P....Recio J.A. (2011)

    External Searches for PRMT5 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing PRMT5 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 10419 HGNC: 10894 AceView: SKB1 Ensembl:ENSG00000100462 euGenes: HUgn10419
    ECgene: PRMT5 Kegg: 10419 H-InvDB: PRMT5

    Other Databases showing PRMT5 gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing PRMT5 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for PRMT5 Pharmacogenomics, SNPs, Pathways

    Intellectual Property
    for PRMT5 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for PRMT5 gene:
    Search GeneIP for patents involving PRMT5

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
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