Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



PRKD3 Gene

protein-coding   GIFtS: 69
GCID: GC02M037477

Protein Kinase D3

(Previous names: protein kinase C, nu)
(Previous symbol: PRKCN)
  Try our

disease database 

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Protein Kinase D31 2     PKC-NU2
PRKCN1 2 3 5     PKD32
Protein Kinase C, Nu1 2     nPKC-NU2
Protein Kinase C Nu Type2 3     Protein-Serine/Threonine Kinase2
Protein Kinase EPK22 3     Serine/Threonine-Protein Kinase D32
EPK22 3     nPKC-nu3
EC 2.7.11.133 8     EC 2.7.118

External Ids:    HGNC: 94081   Entrez Gene: 236832   Ensembl: ENSG000001158257   OMIM: 6070775   UniProtKB: O948063   

Export aliases for PRKD3 gene to outside databases

Previous GC identifers: GC02M037389 GC02M037217


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for PRKD3 Gene:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by
calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein
targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as
major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific
expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC
family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides
in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to
its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires
intact phopholipase C gamma and the involvement of other PKC family members. (provided by RefSeq, Jul 2008)

GeneCards Summary for PRKD3 Gene:
PRKD3 (protein kinase D3) is a protein-coding gene. GO annotations related to this gene include protein kinase C activity and identical protein binding. An important paralog of this gene is PRKD2.

UniProtKB/Swiss-Prot: KPCD3_HUMAN, O94806
Function: Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC.
Involved in resistance to oxidative stress (By similarity)

summary for PRKD3 Gene:
The protein kinase D (PKD) family of serine/threonine protein kinases contains three members; PKD1, PKD2 and
PKD2. These enzymes occupy a unique position in the signal transduction pathway initiated by diacylglycerol
(DAG) and protein kinase C (PKC). PKDs are direct targets of DAG, and also lie downstream of PKC in a novel
signal transduction pathway that is implicated in a variety of biological processes. Structurally, PKDs
contain an N' terminal cysteine-rich domain (CRD), which binds phorbol esters with high affinity and has a
role in mediating PKD translocation to the plasma membrane upon activation. The PH domains have an
autoregulatory phosphorylation site (PKD1 and PKD2 only) and the catalytic domains have a high degree of
homology to myosin light chain and calmodulin-dependent kinases. In the inactivated state, PKD1 and PKD2 are
localized mainly to the cytoplasm, whilst PKD3 is found both in the cytoplasm and nucleus. Kinase activity
of these enzymes is repressed by their CRD and PH domains. PKDs are activated by a variety of stimuli
including regulatory peptides, lysophosphatidic acid, thrombin, PDGF, IGF-1, oxidative stress,
cholecystokinin, Gbetagamma, ATP and more. These stimuli produce a rapid generation of DAG, which induces
CRD-mediated PKD translocation from the cytosol to the plasma membrane. Novel PKCs are also recruited to the
plasma membrane in response to DAG generation. Novel PKCs are allosterically activated by DAG, and
transphosphorylate PKDs. This stabilizes PKD in its active conformation. Activated PKD dissociates from the
plasma membrane, translocates to the cytosol and subsequently to the nucleus. PKDs have been implicated in
fundamental physiological processes including signal transduction, membrane trafficking, and cell survival,
migration, differentiation and proliferation. PKD upregulates the ERK and Ras signaling pathways, and
suppresses the JNK signaling pathway. These enzymes regulate the budding of secretory vesicles from the
trans-Golgi network and promote integrin recruitment to focal adhesions. In addition, PKDs have a role in
regulating apoptosis and have functions in cell survival pathways induced by oxidative stress. They also
have a role in immune regulation. Despite the plethora of physiological processes PKDs are involved in, only
a few direct targets are known. These include kidins220, an integral membrane protein of neuroendocrine
cells, c-Jun and RIN1, a protein that associates with Ras and 14.3.3 and activates the Ras-MEK-ERK pathway.

Gene Wiki entry for PRKD3 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000002.11  NT_022184.16  NC_018913.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the PRKD3 gene promoter:
         Pbx1a   AML1a   Lmo2   POU6F1 (c2)   Tal-1beta   Evi-1   Gfi-1   Cart-1   ITF-2   En-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): PRKD3 promoter sequence
   Search Chromatin IP Primers for PRKD3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PRKD3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2p21   Ensembl cytogenetic band:  2p22.2   HGNC cytogenetic band: 2p21

PRKD3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PRKD3 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M037477:  view genomic region     (about GC identifiers)

Start:
37,477,645 bp from pter      End:
37,551,951 bp from pter
Size:
74,307 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: KPCD3_HUMAN, O94806 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase D3  
Size: 890 amino acids; 100471 Da
Cofactor: Magnesium (By similarity)
1 PDB 3D structure from and Proteopedia for PRKD3:
2D9Z (3D)    
Secondary accessions: D6W587 Q53TR7 Q8NEL8
Alternative splicing: 2 isoforms:  O94806-1   O94806-2   

Explore the universe of human proteins at neXtProt for PRKD3: NX_O94806

Explore proteomics data for PRKD3 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys293
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for PRKD3 (O94806) (see all 31)
     IPNNCSG  VKVPHTF  HSYTRPT  LSHPWLQ 


    See PRKD3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_005804.1  
    ENSEMBL proteins: 
     ENSP00000368356   ENSP00000399540   ENSP00000398743   ENSP00000401839   ENSP00000234179  

    PRKD3 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for PRKD3
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for PRKD3
    GenScript Custom Purified and Recombinant Proteins Services for PRKD3
    Novus Biologicals PRKD3 Proteins
    Sino Biological Recombinant Protein for PRKD3
    Sino Biological Cell Lysate for PRKD3
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for PRKD3 

     
    antibodies-online proteins for PRKD3 (4 products) 

     
    antibodies-online peptides for PRKD3

    PRKD3 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for PRKD3 (PRKD3/nPKC nu)
    Cell Signaling Technology (CST) Antibodies for PRKD3  (PKD3)
    OriGene Antibodies for PRKD3
    OriGene Custom Antibody Services for PRKD3
    Novus Biologicals PRKD3 Antibodies
    Search for Antibodies for PRKD3 at Abcam
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibodies for PRKD3
    antibodies-online antibodies for PRKD3 (86 products) 

    PRKD3 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for PRKD3
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for PRKD3
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for PRKD3 
    antibodies-online kits for PRKD3 (4 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    PLEKH: Pleckstrin homology (PH) domain containing

    IUPHAR Guide to PHARMACOLOGY protein family classification: protein kinase D3
    Protein kinase D (PKD) family

    Selected InterPro protein domains (see all 10):
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR020454 DAG/PE-bd
     IPR011009 Kinase-like_dom
     IPR001849 Pleckstrin_homology

    Graphical View of Domain Structure for InterPro Entry O94806

    ProtoNet protein and cluster: O94806

    1 Blocks protein domain: IPB001849 Pleckstrin-like

    UniProtKB/Swiss-Prot: KPCD3_HUMAN, O94806
    Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily
    Similarity: Contains 1 PH domain
    Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers
    Similarity: Contains 1 protein kinase domain


    Find genes that share domains with PRKD3           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: KPCD3_HUMAN, O94806
    Function: Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC.
    Involved in resistance to oxidative stress (By similarity)
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and
    phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735
    and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain

         Enzyme Numbers (IUBMB): EC 2.7.11.131 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004697protein kinase C activity IEA--
    GO:0004713protein tyrosine kinase activity ----
    GO:0005515protein binding IPI17196367
    GO:0005524ATP binding IEA--
         
    Find genes that share ontologies with PRKD3           About GenesLikeMe


    Phenotypes:
         5 GenomeRNAi human phenotypes for PRKD3:
     Decreased focal adhesion (FA)   Decreased substrate adherent c  Decreased viability with RAF26  G0/1 arrest 
     Increased G1 DNA content 

         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Prkd3):
     limbs/digits/tail  skeleton 

    Find genes that share phenotypes with PRKD3           About GenesLikeMe

    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for PRKD3

    miRNA
    Products:
        
    miRTarBase miRNAs that target PRKD3:
    hsa-mir-148b-3p (MIRT019291), hsa-mir-9-5p (MIRT021381), hsa-mir-20a-5p (MIRT050498), hsa-mir-215-5p (MIRT024592), hsa-mir-192-5p (MIRT026308)

    Block miRNA regulation of human, mouse, rat PRKD3 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PRKD3 (see all 58):
    hsa-miR-323-3p hsa-miR-582-3p hsa-miR-139-5p hsa-miR-301a hsa-miR-192 hsa-miR-548l hsa-miR-105 hsa-miR-9
    SwitchGear 3'UTR luciferase reporter plasmidPRKD3 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for PRKD3
    Predesigned siRNA for gene silencing in human, mouse, rat PRKD3

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for PRKD3

    Clone
    Products:
         
    OriGene clones in human, mouse for PRKD3 (see all 7)
    OriGene ORF clones in mouse, rat for PRKD3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: PRKD3 (NM_005813)
    Sino Biological Human cDNA Clone for PRKD3
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PRKD3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKD3
    Addgene plasmids for PRKD3 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for PRKD3
    Browse ESI BIO Cell Lines and PureStem Progenitors for PRKD3 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKD3


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    KPCD3_HUMAN, O94806: Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase
    activation
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus4
    cytosol3
    cytoskeleton2
    mitochondrion2
    plasma membrane2
    endoplasmic reticulum1

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm IDA--
    GO:0016020membrane IEA--

    Find genes that share ontologies with PRKD3           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for PRKD3 About   (see all 17)  
    See pathways by source

    SuperPathContained pathways About
    1G Protein Signaling Pathways
    G Protein Signaling Pathways0.44
    Signal transduction cAMP signaling0.44
    2Development VEGF signaling and activation
    Development VEGF signaling and activation0.75
    Development VEGF signaling via VEGFR2 generic cascades0.75
    3Non-Canonical Wnt Pathway
    Non-Canonical Wnt Pathway0.53
    4TNF Signaling
    Death Receptor Signaling0.50
    5Immune response Function of MEF2 in T lymphocytes
    Immune response Function of MEF2 in T lymphocytes0.38


    Find genes that share SuperPaths with PRKD3           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for PRKD3
        VEGF - VEGF R2 Signaling Pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for PRKD3 (see all 74)
        UVB-Induced MAPK Signaling
    Molecular Mechanisms of Cancer
    Intracellular Calcium Signaling
    Visual Cycle in Retinal Rods
    NF-kappaB Activation by Viruses

    1 Cell Signaling Technology (CST) Pathway for PRKD3
        Ca, cAMP and Lipid Signaling

    Selected GeneGo (Thomson Reuters) Pathways for PRKD3 (see all 7)
        Signal transduction Erk Interactions- Inhibition of Erk
    Development VEGF signaling via VEGFR2 - generic cascades
    Signal transduction cAMP signaling
    Transcription Transcription factor Tubby signaling pathways
    Development VEGF signaling and activation

    3 BioSystems Pathways for PRKD3
        G Protein Signaling Pathways
    IL-4 signaling Pathway
    miRs in Muscle Cell Differentiation

    5 Sino Biological Pathways for PRKD3
        EGFR Signaling Pathway
    Non-Canonical Wnt Pathway
    VEGF Signaling
    Death Receptor Signaling
    TGF-beta Signaling Pathway


    1 Kegg Pathway  (Kegg details for PRKD3):
        Rap1 signaling pathway

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for PRKD3
    Interactions:

        Search GeneGlobe Interaction Network for PRKD3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 16)

    Selected Interacting proteins for PRKD3 (O948061, 2, 3 ENSP000002341794) via UniProtKB, MINT, STRING, and/or I2D (see all 18)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    NUDT3O959892, 3MINT-8264955 I2D: score=2 
    VAMP2P630271, 3, ENSP000003142144EBI-1255366,EBI-520113 I2D: score=2 STRING: ENSP00000314214
    GSK3AP498403, ENSP000002223304I2D: score=2 STRING: ENSP00000222330
    CHMP243863, ENSP000003503864I2D: score=1 STRING: ENSP00000350386
    KPNB1Q149743, ENSP000002901584I2D: score=1 STRING: ENSP00000290158
    About this table

    Gene Ontology (GO): 4 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006468protein phosphorylation TAS10231560
    GO:0007205protein kinase C-activating G-protein coupled receptor signaling pathway TAS10231560
    GO:0009103lipopolysaccharide biosynthetic process ----
    GO:0035556intracellular signal transduction IEA--

    Find genes that share ontologies with PRKD3           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
    4 ApexBio Compounds for PRKD3     About this table
    CompoundAction CAS #
    CID 2011756novel and potent inhibitor of PKD1[638156-11-3]
    CID 755673Selective PKD inhibitor[521937-07-5]
    CRT 0066101Potent inhibitor of PKD--
    kb NB 142-70selective PKD inhibitor[1233533-04-4]

    Compounds for PRKD3 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    kb NB 142-70Selective PKD inhibitor; analog of CID 755673 (Cat. No. 3327)[1233533-04-4]
    CID 2011756Pan protein kinase D (PKD) inhibitor; cell permeable[638156-11-3]
    CID 755673Selective protein kinase D inhibitor[521937-07-5]

    Selected HMDB Compounds for PRKD3 (see all 846)    About this table
    CompoundSynonyms CAS #PubMed Ids
    DG(16:0e/18:0/0:0)2-Octadecanoyl-1-hexadecyl-sn-glycerol (see all 2)--12486725
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    CalciumCa (see all 2)7440-70-2--
    DG(14:0/14:0/0:0)DAG(14:0/14:0) (see all 9)----
    DG(14:0/14:1(9Z)/0:0)DAG(14:0/14:1) (see all 15)----
    DG(14:0/15:0/0:0)Diacylglycerol(29:0) (see all 9)----
    DG(14:0/16:0/0:0)DAG(30:0) (see all 9)----
    DG(14:0/16:1(9Z)/0:0)DG(30:1) (see all 15)----
    DG(14:0/18:0/0:0)DG(14:0/18:0) (see all 9)----

    2 Novoseek inferred chemical compound relationships for PRKD3 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    threonine 62.8 2 17077180 (1), 16380377 (1)
    serine 59.2 3 17077180 (1), 12506120 (1), 16380377 (1)



    Find genes that share compounds with PRKD3           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for PRKD3 gene: 
    NM_005813.3  

    Unigene Cluster for PRKD3:

    Protein kinase D3
    Hs.660757  [show with all ESTs]
    Unigene Representative Sequence: NM_005813
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000379066(uc002rqd.3) ENST00000469275(uc002rqe.1) ENST00000452104
    ENST00000443977 ENST00000494667(uc002rqf.1) ENST00000475912 ENST00000443187
    ENST00000464552 ENST00000477132 ENST00000234179
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat PRKD3 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PRKD3 (see all 58):
    hsa-miR-323-3p hsa-miR-582-3p hsa-miR-139-5p hsa-miR-301a hsa-miR-192 hsa-miR-548l hsa-miR-105 hsa-miR-9
    SwitchGear 3'UTR luciferase reporter plasmidPRKD3 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for PRKD3
    Predesigned siRNA for gene silencing in human, mouse, rat PRKD3
    Clone
    Products:
         
    OriGene clones in human, mouse for PRKD3 (see all 7)
    OriGene ORF clones in mouse, rat for PRKD3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: PRKD3 (NM_005813)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PRKD3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKD3
    Addgene plasmids for PRKD3 
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for PRKD3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat PRKD3
      QuantiTect SYBR Green Assays in human, mouse, rat PRKD3
      QuantiFast Probe-based Assays in human, mouse, rat PRKD3

    Additional mRNA sequence: 

    AB015982.2 AK027585.1 BC012820.2 BC030706.2 Z25429.1 

    15 DOTS entries:

    DT.448307  DT.99942189  DT.99935034  DT.121001680  DT.40285021  DT.121001603  DT.100749416  DT.121001621 
    DT.75191011  DT.100737529  DT.101972013  DT.92427105  DT.75106802  DT.92427103  DT.92427104 

    Selected AceView cDNA sequences (see all 171):

    BX281412 AI634380 CA392084 AI745310 AI200389 AA400084 AA912626 BM541860 
    AI633542 BM800648 BQ001740 AI096346 AA610099 AA504441 BE892961 BQ218623 
    BC030706 AA279442 AW515068 AI241335 CA392083 AW954114 AL701262 BU173084 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    PRKD3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGAAAACCTG
    PRKD3 Expression
    About this image


    PRKD3 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     
     Gut Tube (Gastrointestinal Tract)
             Pharynx
    PRKD3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    PRKD3 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.660757

    UniProtKB/Swiss-Prot: KPCD3_HUMAN, O94806
    Tissue specificity: Ubiquitous

        Custom PCR Arrays for PRKD3
    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for PRKD3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat PRKD3
    QuantiTect SYBR Green Assays in human, mouse, rat PRKD3
    QuantiFast Probe-based Assays in human, mouse, rat PRKD3
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKD3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for PRKD3 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Prkd31 , 5 protein kinase D31, 5 89.7(n)1
    95.96(a)1
      17 (49.69 cM)5
    752921  NM_001171004.11  NP_001164475.11 
     789494055 
    chicken
    (Gallus gallus)
    Aves PRKD31 protein kinase D3 82.21(n)
    89.78(a)
      421478  XM_419526.4  XP_419526.2 
    lizard
    (Anolis carolinensis)
    Reptilia PRKD36
    protein kinase D3
    89(a)
    1 ↔ 1
    1(235280987-235341315)
    zebrafish
    (Danio rerio)
    Actinopterygii prkd31 protein kinase D3 74.01(n)
    84.34(a)
      557337  XM_680391.5  XP_685483.4 
    fruit fly
    (Drosophila melanogaster)
    Insecta PKD1 Protein Kinase D 55.77(n)
    59.4(a)
      42203  NM_001275776.1  NP_001262705.1 
    worm
    (Caenorhabditis elegans)
    Secernentea dkf-21 dkf-2 55.75(n)
    56.38(a)
      180121  NM_074838.5  NP_507239.2 


    ENSEMBL Gene Tree for PRKD3 (if available)
    TreeFam Gene Tree for PRKD3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for PRKD3 gene
    PRKD22  PRKD12  
    Selected SIMAP similar genes for PRKD3 using alignment to 4 protein entries:     KPCD3_HUMAN (see all proteins) (see all similar genes):
    PRKD1    PRKD2    PRKCH    GRK5    NEK8    SIK3
    BRSK2    MARK4    PRKACA    OBSCN    PHKG2    RPS6KA1
    STK36    NUAK1    PHKG1    IKBKB    RPS6KA3    ULK1

    Find genes that share paralogs with PRKD3           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for PRKD3 (see all 1661)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0423364
    A glioblastoma multiforme sample4--see VAR_0423362 V M mis40--------
    rs2004056141,2
    C,Funtested137506908(+) GGCTTC/TTTCAT 2 K E mis11Minor allele frequency- T:0.02EU 835
    rs1898628641,2
    --37477215(+) TTAGTA/GGTGGT 1 -- ds50010--------
    rs116796171,2
    C,F,A,H--37477243(+) CAAGAC/TAGTTC 1 -- ds50019Minor allele frequency- T:0.35NA WA EA 370
    rs1181017271,2
    F--37477304(+) AGGAAG/AACGGA 1 -- ds50011Minor allele frequency- A:0.01EA 120
    rs739274241,2
    C--37477306(+) GAAGAC/TGGAGG 1 -- ds50013Minor allele frequency- T:0.17WA EA 240
    rs1824901901,2
    --37477391(+) TTTTTC/TCAAAG 1 -- ds50010--------
    rs1505151371,2
    --37477424(+) AAGATG/TAGTTA 1 -- ds50010--------
    rs1867648641,2
    --37477453(+) CTCTCA/GGATAT 1 -- ds50010--------
    rs1394443591,2
    --37477570(+) TAACAG/TTTGGA 1 -- ds50010--------

    HapMap Linkage Disequilibrium report for PRKD3 (37477645 - 37551951 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for PRKD3:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv828953CNV Loss20364138

    Human Gene Mutation Database (HGMD): PRKD3
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing PRKD3
    DNA2.0 Custom Variant and Variant Library Synthesis for PRKD3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 607077    OMIM disorders: --

    1 disease from the University of Copenhagen DISEASES database for PRKD3:
    Polycystic kidney disease

    Find genes that share disorders with PRKD3           About GenesLikeMe

    Genetic Association Database (GAD): PRKD3

    Export disorders for PRKD3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for PRKD3 gene, integrated from 10 sources (see all 70):
    (articles sorted by number of sources associating them with PRKD3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. PKCnu, a new member of the protein kinase C family, composes a fourth subfamily with PKCmu. (PubMed id 10231560)1, 2, 3, 9 Hayashi A.... Saito T. (Biochim. Biophys. Acta 1999)
    2. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)1, 2 Beausoleil S.A.... Gygi S.P. (Nat. Biotechnol. 2006)
    3. The nuclear import of protein kinase D3 requires its catalytic activity. (PubMed id 16380377)1, 9 Rey O....Rozengurt E. (J. Biol. Chem. 2006)
    4. Regulation of protein kinase Cnu by the B-cell antigen receptor. (PubMed id 12506120)1, 9 Matthews S.A....Scharenberg A.M. (J. Biol. Chem. 2003)
    5. Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists. (PubMed id 12676944)1, 9 Rey O....Rozengurt E. (J. Biol. Chem. 2003)
    6. Protein kinase C-independent effects of protein kinase D3 in glucose transport in L6 myotubes. (PubMed id 15496505)1, 9 Chen J....Wang Q.J. (Mol. Pharmacol. 2005)
    7. Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network. (PubMed id 14743217)1, 9 Yeaman C.... Malhotra V. (Nat. Cell Biol. 2004)
    8. Protein kinase D promotes airway epithelial barrier dysfunction and permeability through down-regulation of claudin-1. (PubMed id 24265314)1 Gan H....Tang H. (J. Biol. Chem. 2013)
    9. Protein kinases D2 and D3 are novel growth regulators in HCC1806 triple-negative breast cancer cells. (PubMed id 23393329)1 Hao Q....Tang H. (Anticancer Res. 2013)
    10. Genome-wide association analysis identifies 13 new risk loci for schizophrenia. (PubMed id 23974872)1 Ripke S....Sullivan P.F. (Nat. Genet. 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 23683 HGNC: 9408 AceView: PRKCN Ensembl:ENSG00000115825 euGenes: HUgn23683
    ECgene: PRKD3 Kegg: 23683 H-InvDB: PRKD3

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for PRKD3 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for PRKD3 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for PRKD3 gene:
    Search GeneIP for patents involving PRKD3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Antibodies for PRKD3 (PRKD3/nPKC nu)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for PRKD3   OriGene RNAi products in human, mouse, rat for PRKD3  
     Browse OriGene qPCR primer pairs and template standards   Browse OriGene Protein Over-expression Lysates  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for PRKD3  
     OriGene qSTAR qPCR primer pairs in human, mouse for PRKD3   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for PRKD3   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for PRKD3   OriGene Custom Protein Services for PRKD3  

     
     
     Block miRNA regulation of human, mouse, rat PRKD3 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing PRKD3
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PRKD3 Predesigned siRNA for gene silencing in human, mouse, rat PRKD3
     QuantiFast Probe-based Assays in human, mouse, rat PRKD3 QuantiTect SYBR Green Assays in human, mouse, rat PRKD3
     Custom PCR Arrays for PRKD3 Search Chromatin IP Primers for PRKD3
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat PRKD3  Search GeneGlobe Interaction Network for PRKD3
     Regulatory tfbs in PRKD3 promoter
     GenScript Custom Purified and Recombinant Proteins Services for PRKD3 GenScript cDNA clones with any tag delivered in your preferred vector for PRKD3
     GenScript Custom Assay Services for PRKD3 GenScript Custom overexpressing Cell Line Services for PRKD3
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for PRKD3  (PKD3)

     Tocris compounds for PRKD3
     Proteins for PRKD3
     Cell Lysates for PRKD3
     cDNA Clones for PRKD3
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Proteins for PRKD3
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     PRKD3 antibodies
     PRKD3 proteins
     Search for Antibodies for PRKD3 at Abcam
     See all of Abcam's Antibodies, Kits and Proteins for PRKD3
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Browse Proteins at Cloud-Clone Corp.
     Browse Antibodies at Cloud-Clone Corp.
     Browse ELISAs at Cloud-Clone Corp.
     Browse CLIAs at Cloud-Clone Corp.



     Browse ESI BIO Cell Lines and PureStem Progenitors for PRKD3
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKD3
     SwitchGear 3'UTR luciferase reporter plasmids for PRKD3
     SwitchGear Promoter luciferase reporter plasmids for PRKD3
     ThermoFisher Antibodies for PRKD3
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKD3
     ApexBio compounds for PRKD3
     Addgene plasmids for PRKD3
      Search eBioscience for proteins for PRKD3
      Search eBioscience for elisas for PRKD3
      eBioscience FlowRNA Probe Sets
     genOway: Develop your customized and physiologically relevant rodent model for PRKD3
     antibodies-online antibodies for PRKD3 (86 products)
     antibodies-online kits for PRKD3 (4 products)
     antibodies-online peptides for PRKD3
     antibodies-online proteins for PRKD3 (4 products)
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      PRKD3 gene at Home site.
    Version: 3.12.250 16 Nov 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4