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Aliases for PRKD2 Gene

Aliases for PRKD2 Gene

  • Protein Kinase D2 2 3 5
  • EC 2.7.11.13 4 61
  • NPKC-D2 3 4
  • PKD2 3 4
  • Serine/Threonine-Protein Kinase D2 3
  • EC 2.7.11 61
  • HSPC187 3

External Ids for PRKD2 Gene

Previous GeneCards Identifiers for PRKD2 Gene

  • GC19M047568
  • GC19M051853
  • GC19M051869
  • GC19M047177
  • GC19M043602

Summaries for PRKD2 Gene

Entrez Gene Summary for PRKD2 Gene

  • The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKD2 Gene

PRKD2 (Protein Kinase D2) is a Protein Coding gene. Among its related pathways are Metabolism and Ras signaling pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKD1.

UniProtKB/Swiss-Prot for PRKD2 Gene

  • Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion.

Tocris Summary for PRKD2 Gene

  • The protein kinase D (PKD) family of serine/threonine protein kinases has three members: PKD1-3. They occupy a unique position in signaling pathways initiated by diacylglycerol and protein kinase C. PKDs are involved in cell survival, migration, differentiation and proliferation.

Gene Wiki entry for PRKD2 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKD2 Gene

Genomics for PRKD2 Gene

Regulatory Elements for PRKD2 Gene

Enhancers for PRKD2 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH19F046727 1.8 FANTOM5 Ensembl ENCODE 28.9 -14.2 -14232 6.9 HDGF PKNOX1 MLX ARID4B SIN3A FEZF1 DMAP1 ZNF2 SLC30A9 ZNF302 ZC3H4 GLTSCR1 ENSG00000268810 CCDC61 PRKD2 STRN4 ENSG00000259605 ENSG00000268746 MIR320E GNG8
GH19F047105 1.6 FANTOM5 ENCODE 25.2 -393.2 -393170 9.2 CREB3L1 MLX YBX1 FEZF1 DMAP1 YY1 SLC30A9 ZNF263 SP3 NFYC CCDC9 ZC3H4 C5AR2 PRKD2 GLTSCR1 SNORD23 DHX34 TPRX2P ELSPBP1 GLTSCR2-AS1
GH19F046782 1.2 ENCODE 31.3 -68.7 -68655 6.8 CREB3L1 MLX FEZF1 DMAP1 YY1 ZNF143 ZNF263 SP3 NFYC ZFP41 PRKD2 SLC1A5 CCDC61 STRN4 SNORD23 PPP5D1 IGFL4 LOC93429 LOC105372421 ZC3H4
GH19F047239 1.9 FANTOM5 Ensembl ENCODE 18.9 -525.1 -525074 6.4 CREB3L1 ZFP64 DMAP1 YBX1 YY1 SLC30A9 ZNF143 ZNF416 ZNF548 ZNF263 ZC3H4 GLTSCR1 ENSG00000268810 ENSG00000269321 CCDC9 GLTSCR2-AS1 EHD2 SLC1A5 PRKD2 TPRX2P
GH19F047225 1.2 ENCODE 27.1 -512.3 -512255 8.2 MLX ZFP64 FEZF1 DMAP1 YY1 ZNF416 ZNF143 ZNF548 ZNF263 SP3 ZC3H4 GLTSCR1 CCDC9 ENSG00000268810 GLTSCR2-AS1 ENSG00000269321 SLC1A5 PRKD2 SNORD23 ENSG00000268746
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around PRKD2 on UCSC Golden Path with GeneCards custom track

Promoters for PRKD2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000347635 1527 4401 PKNOX1 ATF1 ARID4B SIN3A DMAP1 ZNF48 ZNF121 GTF3C2 ZNF143 PAF1

Genomic Location for PRKD2 Gene

Chromosome:
19
Start:
46,674,275 bp from pter
End:
46,717,127 bp from pter
Size:
42,853 bases
Orientation:
Minus strand

Genomic View for PRKD2 Gene

Genes around PRKD2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRKD2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRKD2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKD2 Gene

Proteins for PRKD2 Gene

  • Protein details for PRKD2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9BZL6-KPCD2_HUMAN
    Recommended name:
    Serine/threonine-protein kinase D2
    Protein Accession:
    Q9BZL6
    Secondary Accessions:
    • M0R2R2
    • Q8NCK8
    • Q8TB08
    • Q9P0T6
    • Q9Y3X8

    Protein attributes for PRKD2 Gene

    Size:
    878 amino acids
    Molecular mass:
    96750 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts (via C-terminus) with LCK. Interacts (via N-terminal AP-rich region) with CIB1 isoform 2.
    SequenceCaution:
    • Sequence=AAF36107.1; Type=Frameshift; Positions=604, 606, 738, 744, 755, 758, 833, 854; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for PRKD2 Gene

    Alternative splice isoforms for PRKD2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PRKD2 Gene

Selected DME Specific Peptides for PRKD2 Gene

Q9BZL6:
  • AIDLINNLLQVKMRKRYSVDK
  • QCKDCKFNCHKRCA
  • LHFKNIVHCDLKPENVLLASA
  • GCGLNYHKRCAF
  • QIQENVDI
  • VFVVMEKLHGDMLEMILSSEKGRLPE
  • HSYTRPT
  • GLVRQGL
  • AVKVIDK
  • YLAPEVL
  • YDKILLF
  • VNLECMFETPE
  • IPNNCSG
  • VKVPHTF
  • RQGLQCK
  • LLKGLFRQG
  • EDFQIRPH
  • GYNRSLDMWSVGVI
  • GERYITHESDD
  • QKFPECGF
  • EGDLVEVVLSA
  • YYKEIPLSEIL
  • TQILVALR
  • VYGGKHRKTGRDVA
  • YVSLSGTFPFNEDEDI
  • DMWSVGVI
  • LSHPWLQ
  • DQIQNAAFMYP
  • AIRQALMPV
  • KCITLFQN

Post-translational modifications for PRKD2 Gene

  • Phosphorylation of Ser-876 correlates with the activation status of the kinase. Ser-706 is probably phosphorylated by PKC. Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting. Phosphorylation at Ser-244, Ser-706 and Ser-710 is required for nuclear localization. Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress.
  • Ubiquitination at Lys 178
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PRKD2 (PRKD2)
  • Cloud-Clone Corp. Antibodies for PRKD2

Domains & Families for PRKD2 Gene

Gene Families for PRKD2 Gene

Suggested Antigen Peptide Sequences for PRKD2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9BZL6

UniProtKB/Swiss-Prot:

KPCD2_HUMAN :
  • Contains 1 PH domain.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
Domain:
  • Contains 1 PH domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Similarity:
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
genes like me logo Genes that share domains with PRKD2: view

Function for PRKD2 Gene

Molecular function for PRKD2 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain.
UniProtKB/Swiss-Prot Function:
Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion.

Enzyme Numbers (IUBMB) for PRKD2 Gene

Gene Ontology (GO) - Molecular Function for PRKD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IDA 17077180
GO:0004674 protein serine/threonine kinase activity NAS,IDA 11062248
GO:0004697 protein kinase C activity IEA --
GO:0005515 protein binding IPI 17962809
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with PRKD2: view
genes like me logo Genes that share phenotypes with PRKD2: view

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for PRKD2 Gene

Localization for PRKD2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRKD2 Gene

Cytoplasm. Cell membrane. Nucleus. Golgi apparatus, trans-Golgi network. Note=Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. {ECO:0000269 PubMed:17962809}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PRKD2 gene
Compartment Confidence
nucleus 5
cytosol 5
plasma membrane 4
golgi apparatus 4

Gene Ontology (GO) - Cellular Components for PRKD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IEA --
GO:0005634 nucleus IDA 17077180
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IDA 17077180
GO:0005794 Golgi apparatus IEA --
genes like me logo Genes that share ontologies with PRKD2: view

Pathways & Interactions for PRKD2 Gene

genes like me logo Genes that share pathways with PRKD2: view

SIGNOR curated interactions for PRKD2 Gene

Activates:
Inactivates:
Is activated by:

Gene Ontology (GO) - Biological Process for PRKD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001525 angiogenesis IEA --
GO:0001938 positive regulation of endothelial cell proliferation IGI,IMP 19001381
GO:0002250 adaptive immune response IEA --
GO:0002376 immune system process IEA --
GO:0006468 protein phosphorylation NAS,IDA 11062248
genes like me logo Genes that share ontologies with PRKD2: view

Drugs & Compounds for PRKD2 Gene

(11) Drugs for PRKD2 Gene - From: ApexBio, DGIdb, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
CID 2011756 Pharma PKD inhibitor, Pan protein kinase D (PKD) inhibitor; cell permeable 0
CID 755673 Pharma PKD inhibitor, Selective protein kinase D inhibitor 0
bryostatin Pharma inhibitor 0
BRYOSTATIN-1 Pharma 0

(5) Additional Compounds for PRKD2 Gene - From: HMDB