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Aliases for PRKCQ Gene

Aliases for PRKCQ Gene

  • Protein Kinase C Theta 2 3 5
  • EC 2.7.11.13 4 61
  • NPKC-Theta 3 4
  • PRKCT 3 4
  • Protein Kinase C Theta Type 3
  • Protein Kinase C, Theta 2
  • EC 2.7.11 61

External Ids for PRKCQ Gene

Previous GeneCards Identifiers for PRKCQ Gene

  • GC10M006413
  • GC10M006619
  • GC10M006509

Summaries for PRKCQ Gene

Entrez Gene Summary for PRKCQ Gene

  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKCQ Gene

PRKCQ (Protein Kinase C Theta) is a Protein Coding gene. Diseases associated with PRKCQ include Gastrointestinal Stromal Tumor and Smallpox. Among its related pathways are Metabolism of fat-soluble vitamins and VEGF Signaling Pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKCD.

UniProtKB/Swiss-Prot for PRKCQ Gene

  • Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in outside-in signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at Ser-504 and Ser-532 and negatively regulates its ability to phosphorylate PKB/AKT1.

Tocris Summary for PRKCQ Gene

  • Protein kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanism. Conventional PKCs (cPKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol.

Gene Wiki entry for PRKCQ Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKCQ Gene

Genomics for PRKCQ Gene

Regulatory Elements for PRKCQ Gene

Enhancers for PRKCQ Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH10F006528 1 FANTOM5 Ensembl ENCODE 27.2 +50.0 49991 4.1 ZNF146 PRKCQ PRKCQ-AS1 GC10M006507 GC10M006525
GH10F006541 1.3 FANTOM5 Ensembl ENCODE 19.9 +36.7 36714 5.1 HDAC1 PKNOX1 CREB3L1 BMI1 ZNF316 STAT2 ETV6 STAT1 MTA2 HLF PRKCQ PRKCQ-AS1 GC10M006507 GC10P006574
GH10F006470 1.5 FANTOM5 Ensembl ENCODE 12.6 +107.1 107115 5.2 PKNOX1 ATF1 ARNT ZNF766 ELK1 GATA2 KLF13 NCOA1 TBX21 CREB3 PRKCQ PRKCQ-AS1 PFKFB3 IL15RA RBM17 GC10P006490 GC10P006453
GH10F006590 1.2 FANTOM5 Ensembl ENCODE 15.2 -12.2 -12193 3.7 ZBTB10 ZNF263 MTA2 SCRT2 TBX21 EP300 PRKCQ GC10M006583 GC10P006595
GH10F006497 1.1 FANTOM5 Ensembl ENCODE 15.2 +81.6 81584 2.4 PKNOX1 ZNF26 CEBPB PRKCQ NRBF2P5 LOC105376384 PRKCQ-AS1 PFKFB3 RBM17 GC10P006490 GC10M006525
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around PRKCQ on UCSC Golden Path with GeneCards custom track

Promoters for PRKCQ Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000350235 546 3401 ATF1 SIN3A DMAP1 ZNF2 ZNF48 GLIS2 FOS ZNF263 SP3 REST

Genomic Location for PRKCQ Gene

Chromosome:
10
Start:
6,393,595 bp from pter
End:
6,580,646 bp from pter
Size:
187,052 bases
Orientation:
Minus strand

Genomic View for PRKCQ Gene

Genes around PRKCQ on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRKCQ Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRKCQ Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKCQ Gene

Proteins for PRKCQ Gene

  • Protein details for PRKCQ Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q04759-KPCT_HUMAN
    Recommended name:
    Protein kinase C theta type
    Protein Accession:
    Q04759
    Secondary Accessions:
    • B4DF52
    • Q14DH6
    • Q3MJF1
    • Q64FY5
    • Q9H508
    • Q9H549

    Protein attributes for PRKCQ Gene

    Size:
    706 amino acids
    Molecular mass:
    81865 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with GLRX3 (via N-terminus). Interacts with ECT2. Part of a lipid raft complex composed at least of BCL10, CARD11, MALT1 and IKBKB.

    Three dimensional structures from OCA and Proteopedia for PRKCQ Gene

    Alternative splice isoforms for PRKCQ Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PRKCQ Gene

Selected DME Specific Peptides for PRKCQ Gene

Q04759:
  • KVANLCGINQKL
  • PPFRPKVKSP
  • GKGSFGKV
  • GHIKIAD
  • DDDVECTMVEKRVL
  • WGLNKQGY
  • GVLLYEM
  • DFGMCKE
  • IDMPHRFK
  • VMEYLNGGD
  • HCGTLLWGL
  • FAIKALKK
  • YRDLKLDN
  • GTPDYIAPE
  • LDKDGHIK
  • CNAAIHKKCIDK
  • TFCGTPDYIAPEI
  • QKKPTMYP
  • VDWWSFGVLLYEML
  • YIAPEIL
  • GQSPFHG
  • RDLKLDN

Post-translational modifications for PRKCQ Gene

  • Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation. Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685.
  • Ubiquitination at Lys 77, Lys 110, Lys 286, Lys 325, Lys 384, and Lys 527
  • Modification sites at PhosphoSitePlus

Domains & Families for PRKCQ Gene

Graphical View of Domain Structure for InterPro Entry

Q04759

UniProtKB/Swiss-Prot:

KPCT_HUMAN :
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
Domain:
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Contains 1 AGC-kinase C-terminal domain.
  • Contains 1 C2 domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Similarity:
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
genes like me logo Genes that share domains with PRKCQ: view

Function for PRKCQ Gene

Molecular function for PRKCQ Gene

GENATLAS Biochemistry:
protein kinase C,theta polypeptide,critically involved in the regulation of differentiation and proliferation of T lymphocytes
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.
UniProtKB/Swiss-Prot Function:
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in outside-in signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at Ser-504 and Ser-532 and negatively regulates its ability to phosphorylate PKB/AKT1.

Enzyme Numbers (IUBMB) for PRKCQ Gene

Gene Ontology (GO) - Molecular Function for PRKCQ Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA,TAS --
GO:0004674 protein serine/threonine kinase activity IEA,TAS --
GO:0004697 protein kinase C activity IEA --
GO:0005515 protein binding IPI 10383400
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with PRKCQ: view
genes like me logo Genes that share phenotypes with PRKCQ: view

Animal Models for PRKCQ Gene

MGI Knock Outs for PRKCQ:

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for PRKCQ Gene

Localization for PRKCQ Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRKCQ Gene

Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PRKCQ gene
Compartment Confidence
plasma membrane 5
cytosol 5
cytoskeleton 4
nucleus 2

Gene Ontology (GO) - Cellular Components for PRKCQ Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001772 immunological synapse IEA --
GO:0005622 intracellular IEA,IBA --
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane TAS --
genes like me logo Genes that share ontologies with PRKCQ: view

Pathways & Interactions for PRKCQ Gene

genes like me logo Genes that share pathways with PRKCQ: view

Pathways by source for PRKCQ Gene

SIGNOR curated interactions for PRKCQ Gene

Activates:
Is activated by:
Other effect:

Gene Ontology (GO) - Biological Process for PRKCQ Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001558 regulation of cell growth NAS 8444877
GO:0002376 immune system process IEA --
GO:0006355 regulation of transcription, DNA-templated ISS --
GO:0006468 protein phosphorylation IEA --
GO:0006509 membrane protein ectodomain proteolysis ISS --
genes like me logo Genes that share ontologies with PRKCQ: view

Drugs & Compounds for PRKCQ Gene

(19) Drugs for PRKCQ Gene - From: DrugBank, DGIdb, FDA Approved Drugs, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Tamoxifen Approved Pharma Antagonist, Target TGF-β modulatory and PKC inhibitory effects, ER antagonist, Anti-Estrogens 380
Ingenol Mebutate Approved Pharma 0
nolvadex Approved October 1998 Pharma 0
Adenosine triphosphate Approved Nutra 0
Phosphonoserine Experimental Pharma Target 0

(7) Additional Compounds for PRKCQ Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group C
  • 1-Myristoyl-3-stearoyl-sn-glycerol
  • 1-Tetradecanoyl-3-octadecanoyl-sn-glycerol
  • DAG(14:0/0:0/18:0)
  • DAG(32:0)
  • DG(32:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)

(5) Tocris Compounds for PRKCQ Gene

Compound Action Cas Number
Enzastaurin Potent PKCbeta inhibitor 170364-57-5
GF 109203X Protein kinase C inhibitor 133052-90-1
Go 6976 Potent protein kinase C inhibitor; selective for alpha and beta isozymes 136194-77-9
Go 6983 Broad spectrum PKC inhibitor 133053-19-7
Phorbol 12-myristate 13-acetate Protein kinase C activator 16561-29-8
genes like me logo Genes that share compounds with PRKCQ: view

Transcripts for PRKCQ Gene

Unigene Clusters for PRKCQ Gene

Protein kinase C, theta:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PRKCQ Gene

ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b
SP1: - -
SP2: -
SP3: -

Relevant External Links for PRKCQ Gene

GeneLoc Exon Structure for
PRKCQ
ECgene alternative splicing isoforms for
PRKCQ

Expression for PRKCQ Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PRKCQ Gene

mRNA differential expression in normal tissues according to GTEx for PRKCQ Gene

This gene is overexpressed in Muscle - Skeletal (x18.3) and Whole Blood (x4.7).

Protein differential expression in normal tissues from HIPED for PRKCQ Gene

This gene is overexpressed in Peripheral blood mononuclear cells (47.7) and Heart (6.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PRKCQ Gene



Protein tissue co-expression partners for PRKCQ Gene

NURSA nuclear receptor signaling pathways regulating expression of PRKCQ Gene:

PRKCQ

SOURCE GeneReport for Unigene cluster for PRKCQ Gene:

Hs.498570

mRNA Expression by UniProt/SwissProt for PRKCQ Gene:

Q04759-KPCT_HUMAN
Tissue specificity: Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.
genes like me logo Genes that share expression patterns with PRKCQ: view

Primer Products

Orthologs for PRKCQ Gene

This gene was present in the common ancestor of animals.

Orthologs for PRKCQ Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PRKCQ 34 35
  • 99.58 (n)
dog
(Canis familiaris)
Mammalia PRKCQ 34 35
  • 90.84 (n)
cow
(Bos Taurus)
Mammalia PRKCQ 34 35
  • 89.19 (n)
rat
(Rattus norvegicus)
Mammalia Prkcq 34
  • 87.57 (n)
mouse
(Mus musculus)
Mammalia Prkcq 34 16 35
  • 87.33 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia PRKCQ 35
  • 86 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia PRKCQ 35
  • 84 (a)
OneToOne
chicken
(Gallus gallus)
Aves