PRKCI Gene
protein-coding GIFtS: 70
GCID: GC03P169940
|
|
protein kinase C, iota
(Previous symbol: DXS1179E)
| |
Aliases
for PRKCI gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Protein Kinase C, Iota1 2 | | APKC-Lambda/Iota1 | | DXS1179E1 2 3 | | NPKC-Iota1 | | PKCI1 2 | | EC 2.7.11.133 8 | | Atypical Protein Kinase C-Lambda/Iota2 3 | | Protein Kinase C Iota Type2 | | PRKC-Lambda/Iota1 | | EC 2.7.118 |
Export aliases for PRKCI gene to outside databasesPrevious GC identifers: GC0XM096083 GC03P170989 GC03P171342 GC03P171261 GC03P171422 GC03P167310 |
Summaries
for PRKCI gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for PRKCI:
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC familycomprises at least eight members, which are differentially expressed and are involved in a wide variety of cellularprocesses. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolestersor diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with thesmall GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays arole in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediatedresistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is asingle exon pseudogene mapped on chromosome X. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: KPCI_HUMAN, P41743Function: Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective roleagainst apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, andcontributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABLoncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-inducedapoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process ata very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a proteincomplex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity byphosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF),acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B andneuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR.This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial celldifferentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cellpolarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDHand recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activatedby saturated fatty acids and mediates lipid-induced apoptosis
 summary
for PRKCI: Protein kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation
mechanism. Classical or conventional PKCs (cPKC alpha-, betaI- , betaII- and gamma-) are activated by
phosphatidylserine in a calcium dependent manner and can bind diacylglycerol (DAG). The Ca2+ insensitive
novel PKCs (nPKCs epsilon-, delta-, theta- and eta- isotypes) are also activated by DAG and
phosphatidylserine. The atypical PKCs (aPKCs iota- and zeta-) are insensitive to Ca2+, DAG and phorbol
esters. All PKCs isoforms consist of a highly conserved catalytic domain connected to a regulatory domain
via a hinge region. The physiological roles of PKCs are vast. This due to PKC phosphorylation and activation
of multiple target proteins, which are involved in signal transduction pathways including, but not limited
to, receptor desensitization, modulation of membrane structure events, regulation of transcription,
regulation of cell growth, immune responses, and in learning and memory.
Gene Wiki entry for PRKCI
|
Genomic Views
for PRKCI gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000003.11 NC_018914.1 NT_005612.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the PRKCI gene promoter:
NF-1 NF-1/L NF-AT AREB6 NF-AT4 Pax-3 NF-AT2 FOXO4 NF-AT3 NF-AT1
Other transcription factors
Search SABiosciences Chromatin IP Primers for PRKCI
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PRKCI |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 3q26.3 Ensembl cytogenetic band: 3q26.2 HGNC cytogenetic band: 3q26.3PRKCI Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03P169940: view genomic region
(about GC identifiers)
Start:
|
169,940,153 bp from pter |
End:
|
170,023,770 bp from pter |
Size:
|
83,618 bases |
Orientation:
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plus strand |
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Proteins
for PRKCI gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: KPCI_HUMAN, P41743 (See
protein sequence)Recommended Name: Protein kinase C iota type Size: 596 amino acids; 68262 Da
Subunit: Forms a complex with SQSTM1 and MP2K5 (By similarity). Interacts directly with SQSTM1 (Probable). Interactswith IKBKB. Interacts with PARD6A, PARD6B and PARD6G. Part of a quaternary complex containing aPKC, PARD3, a PARD6protein (PARD6A, PARD6B or PARD6G) and a GTPase protein (CDC42 or RAC1). Part of a complex with LLGL1 and PARD6B.Interacts with ADAP1/CENTA1. Interaction with SMG1, through the ZN-finger domain, activates the kinase activity.Interacts with CDK7. Forms a complex with RAB2A and GAPDH involved in recruitment onto the membrane of vesiculartubular clusters (VTCs). Interacts with ECT2 ('Thr-359' phosphorylated form)
Subcellular location: Cytoplasm. Membrane. Endosome. Nucleus. Note=Transported into the endosome through interactionwith SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1.Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) throughinteraction with RAB2A
Sequence caution: Sequence=AAA60171.1; Type=Erroneous initiation; Note=Translation N-terminally extended;Sequence=AAB17011.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH22016.3;Type=Erroneous initiation; Note=Translation N-terminally extended;
5 PDB 3D structures from  and Proteopedia  for PRKCI:1VD2 (3D)
1WMH (3D)
1ZRZ (3D)
3A8W (3D)
3A8X (3D)
Secondary accessions: D3DNQ4 Q8WW06Explore the universe of human proteins at neXtProt for PRKCI: NX_P41743
Post-translational modifications:
Phosphorylation at Thr-412 in the activation loop is not mandatory for activation (By similarity). Upon neuronal growthfactor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr-280 and Tyr-334. Phosphorylation at Tyr-265facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 isimportant for NF-kappa-B stimulation1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P41743
4/29 DME Specific Peptides for PRKCI (P41743) (see all 29)
PRKCI Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_002731.4
ENSEMBL proteins: ENSP00000295797 Reactome Protein details: P41743
Human Recombinant Protein Products:
Gene Ontology (GO): 5/10 cellular component terms (GO ID links to tree view) (see all 10): About this table
PRKCI for ontologies About GeneDecksing
PRKCI Antibody Products:
Assay Products for PRKCI: |
Protein
Domains /
Families
for PRKCI gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
PRKCI for domains About GeneDecksing
5/11 InterPro domains/families (see all 11):Graphical View of Domain Structure for InterPro Entry P41743ProtoNet protein and cluster: P41743 3 Blocks protein families: IPB000270 Octicosapeptide/Phox/Bem1p IPB000961 Protein kinase C-terminal domain IPB002219 Protein kinase C
UniProtKB/Swiss-Prot: KPCI_HUMAN, P41743Domain: The OPR domain mediates interaction with SQSTM1 (By similarity)Domain: The C1 zinc finger does not bind diacylglycerol (DAG)Domain: The pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins, except thepresence of a non-phosphorylatable residue in place of Ser, it modulates activity by competing with substrates (Bysimilarity)Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamilySimilarity: Contains 1 AGC-kinase C-terminal domainSimilarity: Contains 1 OPR domainSimilarity: Contains 1 phorbol-ester/DAG-type zinc fingerSimilarity: Contains 1 protein kinase domain |
Function
for PRKCI gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: KPCI_HUMAN, P41743Function: Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective roleagainst apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, andcontributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABLoncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-inducedapoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process ata very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a proteincomplex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity byphosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF),acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B andneuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR.This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial celldifferentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cellpolarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDHand recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activatedby saturated fatty acids and mediates lipid-induced apoptosisCatalytic activity: ATP + a protein = ADP + a phosphoproteinEnzyme regulation: Atypical PKCs (PRKCI and PRKCZ) exhibit an elevated basal enzymatic activity (that may be due to theinteraction with SMG1 or SQSTM1) and are not regulated by diacylglycerol, phosphatidylserine, phorbol esters orcalcium ions. Two specific sites, Thr-412 (activation loop of the kinase domain) and Thr-564 (turn motif), need to bephosphorylated for its full activation (By similarity). Might also be a target for novel lipid activators that areelevated during nutrient-stimulated insulin secretionBiophysicochemical properties: Kinetic parameters: KM=13.5 uM for ATP (for recombinant purified PRKCI); Vmax=7.4pmol/min/mg enzyme; Genatlas biochemistry entry for PRKCI:protein kinase C,iota polypeptide
Enzyme Numbers (IUBMB): EC 2.7.11.131 2 EC 2.7.112
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PRKCI (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PRKCI (see all 3)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: PRKCI (NM_002740) | |  | Sino Biological Human cDNA Clone for PRKCI | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PRKCI | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKCI |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKCI |
Gene Ontology (GO): 5/8 molecular function terms (GO ID links to tree view) (see all 8): About this table
PRKCI for ontologies About GeneDecksing
2 GenomeRNAi human phenotypes for PRKCI:
Animal Models:
Mouse knock-outs for PRKCI: Prkcitm1Hed Prkcitm1.1Kido
15 MGI mutant phenotypes (inferred from 4 alleles ) (MGI details for Prkci):
PRKCI for phenotypes About GeneDecksing
|
Pathways & Interactions
for PRKCI gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/66 super-pathways (see all 66) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | ErbB4 Pathway | | | 2 | CCR3 Pathway in Eosinophils | | | 3 | Cell-cell junction organization | | | 4 | Sweet Taste Signaling | | | 5 | Wnt Signaling Pathway | |
Pathway sources
See GeneCards unified pathways
Show all pathways
5/9 EMD Millipore Pathways for PRKCI (see all 9)
2  R&D Systems Pathways for PRKCI
5/68 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for PRKCI (see all 68)
1 
Cell Signaling Technology (CST) Pathway for PRKCI
2 Tocris Bioscience Pathways for PRKCI
5/8  GeneGo (Thomson Reuters) Pathways for PRKCI (see all 8)
5/13  BioSystems Pathways for PRKCI (see all 13)
5/9 
Reactome Pathways for PRKCI (see all 9)
2  PharmGKB Pathways for PRKCI
3 
Kegg Pathways (Kegg details for PRKCI):
PRKCI for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for PRKCI
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/145 Interacting proteins for PRKCI (P417431, 2, 3 ENSP000002957974) via UniProtKB, MINT, STRING, and/or I2D (see all 145)| Interactant | Interaction Details |
|---|
| GeneCard | External ID(s) |
|---|
| SQSTM1 | Q135012, 3, ENSP000003744554 | MINT-8075920 MINT-8075934 MINT-8075945 I2D:
score=8 STRING: ENSP00000374455 | | PARD3 | Q8TEW01, 3, ENSP000003639214 | EBI-286199,EBI-1221123 I2D:
score=7 STRING: ENSP00000363921 | | MARK4 | Q96L341, 3, ENSP000003008434 | EBI-286199,EBI-302319 I2D:
score=4 STRING: ENSP00000300843 | | TSC22D1 | Q157142, 3 | MINT-8275479 I2D:
score=2 | | TTR | P027662, 3 | MINT-8275869 I2D:
score=2 |
About this table
Gene Ontology (GO): 5/27 biological process terms (GO ID links to tree view) (see all 27): About this table
PRKCI for ontologies About GeneDecksing
|
Drugs & Compounds
for PRKCI gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
PRKCI for compounds About GeneDecksing
Compounds for PRKCI available from Tocris Bioscience About this table
| Compound | Action |
CAS
# |
|---|
| [Glu27]-PKC (19-36) | Inactive control peptide for PKC (19-36) (Cat. No. 4058) | [309247-49-2] | | Phorbol 12,13-dibutyrate | Protein kinase C activator | [37558-16-0] | | GF 109203X | Protein kinase C inhibitor | [133052-90-1] | | Go 6976 | Potent protein kinase C inhibitor; selective for alpha and beta isozymes | [136194-77-9] | | PKC (19-36) | Pseudosubstrate peptide; inhibitor of PKC | [113731-96-7] |
10/846 HMDB Compounds for PRKCI (see all 846) About this table
1 DrugBank Compound for PRKCI About this table
1 Novoseek chemical compound relationship for PRKCI gene About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| gf 109203x |
52.5 |
1 |
10101246 (1) |
Search CenterWatch for drugs/clinical trials and news about PRKCI / KPCI
|
Transcripts
for PRKCI gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for PRKCI gene: NM_002740.5 Unigene Cluster for PRKCI: Protein kinase C, iota Hs.478199 [show with all ESTs]Unigene Representative Sequence: NM_0027407 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000295797(uc003fgs.2) ENST00000488541 ENST00000493761 ENST00000482353
ENST00000476635(uc003fgt.2) ENST00000485837 ENST00000483697
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PRKCI (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PRKCI (see all 3)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: PRKCI (NM_002740) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PRKCI | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKCI |
Additional cDNA sequence: AB451443.1 AK315342.1 BC022016.2 BC042405.1 L18964.1 L33881.1 12 DOTS entries: DT.451894 DT.100812320 DT.92326630 DT.121318585 DT.423329 DT.92438678 DT.95140902 DT.97769185 DT.120911514 DT.92438672 DT.99980092 DT.40200257 24/149 AceView cDNA sequences (see all 149): CB135386 AI783813 BM670115 AA479362 BU742623 AI279471 AI920793 AW236231 BU187127 BG179816 BQ006303 BU509388 AI480090 T28044 BQ014217 BG776886 AI754623 BU071082 BM704346 BX108531 BM840658 NM_002740 CK822800 BM767350
GeneLoc Exon Structure
|
Expression
for PRKCI gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| PRKCI expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: ATAAAACATT
About this image
See PRKCI Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for PRKCI
SOURCE GeneReport for Unigene cluster: Hs.478199
UniProtKB/Swiss-Prot: KPCI_HUMAN, P41743Tissue specificity: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissuesincluding pancreatic islets. Highly expressed in non-small cell lung cancers
SABiosciences Expression via Pathway-Focused PCR Arrays including PRKCI:
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PRKCI
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat PRKCI | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PRKCI | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PRKCI | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKCI |
Orthologs
for PRKCI gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for PRKCI gene from 6/24 species (see all 24) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
PRKCI1 |
protein kinase C, iota |
83.93(n)
95.07(a) |
|
424991 NM_001031319.1 NP_001026490.1 |
lizard
(Anolis carolinensis) |
Reptilia |
PRKCI6 |
-- |
96(a) |
1 ↔ 1 |
3(7639969-7670349) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.9672 |
Xenopus laevis protein kinase C subspecies lambda/iota mRNA, complete cds less |
81.18(n) |
|
U12588.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
prkci2 |
protein kinase C, iota |
79.19(n) |
|
117507 AF390109.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
aPKC1 , 3 |
oocyte determination protein
serine/threonine kinase less3
atypical protein kinase C1 |
67(a)
(best of 2)3
63.16(n)1
69.83(a)1 |
|
475941 NM_001043076.11 NP_001036541.11 |
worm
(Caenorhabditis elegans) |
Secernentea |
pkc-31 |
Protein PKC-3 |
60.28(n)
60.28(a) |
|
173914 NM_062610.5 NP_495011.1 |
ENSEMBL Gene Tree for PRKCI (if available)
TreeFam Gene Tree for PRKCI (if available) |
Paralogs
for PRKCI gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for PRKCI gene
- PRKCQ2 PRKCH2 PRKCB2 PRKCE2 PKN12 PRKCA2 PRKCZ2 PRKCD2
- PKN32 PRKCG2 PKN22
18/53 SIMAP similar genes for PRKCI using alignment to 2 protein entries: KPCI_HUMAN (see all proteins)
(see all similar genes):PRKCZ MARK4 PRKCB AKT1 AKT3 KIN27
PRKCA RPS6KB2 AKT2 SGK2 PRKCG RPS6KB1
NUAK1 SGK1 PRKACB PRKCH PRKCE RPS6KA3
PRKCI for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for PRKCI
PGOHUM00000241470
|
Genomic Variants
for PRKCI gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 3 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for PRKCI (169940153 - 170023770 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for PRKCI
2 CNVs: 7384 59109 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PRKCI |
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Disorders
/ Diseases
for PRKCI gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
PRKCI for disorders About GeneDecksing
OMIM gene information: 600539
OMIM disorders: --
20/24  diseases for PRKCI (see all 24): About MalaCardsinterleukin receptor leukemia esophageal squamous cell carcinoma squamous cell carcinoma
hepatocellular carcinoma microphthalmia osteoporosis carcinoma
esophagitis ovarian cancer gastric cancer prostate carcinoma
cholangiocarcinoma osteosarcoma lung cancer prostatitis
prostate cancer breast cancer glioblastoma immunodeficiency
4 Novoseek disease relationships for PRKCI gene About this table
Human Genome Epidemiology (HuGE) Navigator: PRKCI (2 documents)
Export disorders for PRKCI gene to outside databases
|
Publications
for PRKCI gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for PRKCI gene, integrated from 9 sources (see all 174):
(articles sorted by number of sources associating them with PRKCI) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Human protein kinase C iota gene (PRKCI) is closely linked to the BTK gene in Xq21.3. (PubMed id 7607695)1, 2, 3, 9 Mazzarella R.... D'Esposito M. (1995)
- Protein kinase C iota mediates lipid-induced apoptosi s of human coronary artery endothelial cells. (PubMed id 19327373)1, 2, 9 Staiger K....Kellerer M. (2009)
- Crystal structure of the catalytic domain of human atypical protein kinase C-iota reveals interaction mode of phosphorylation site in turn motif. (PubMed id 16125198)1, 2, 9 Messerschmidt A.... Blaesse M. (2005)
- Atypical protein kinase C iota protects human leukemia cells against drug-induced apoptosis. (PubMed id 9346882)1, 2, 9 Murray N.R. and Fields A.P. (1997)
- Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC- iota in human glioma cells. (PubMed id 15695176)1, 2, 9 Bicaku E.... Acevedo-Duncan M. (2005)
- Regulation of interleukin receptor-associated kinase (IRAK) phosphorylation and signaling by iota protein kinase C. (PubMed id 14684752)1, 2, 9 Mamidipudi V....Wooten M.W. (2004)
- Oncogenic activity of Ect2 is regulated through prote in kinase C iota-mediated phosphorylation. (PubMed id 21189248)1, 2 Justilien V....Fields A.P. (2011)
- PKC-? promotes glioblastoma cell survival by phosphorylating and inhibiting BAD through a phosphatidylinositol 3-kinase pathway. (PubMed id 21419810)1, 2 Desai S.... Acevedo-Duncan M. (2011)
- Ect2 links the PKCiota-Par6alpha complex to Rac1 acti vation and cellular transformation. (PubMed id 19617897)1, 2 Justilien V. and Fields A.P. (2009)
- Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells. (PubMed id 18270268)1, 2 Wald F.A....Salas P.J. (2008)
|
External Searches for PRKCI gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing PRKCI gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
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|
|
Other Databases showing PRKCI gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PRKCI gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for PRKCI gene:
Search GeneIP for patents involving PRKCI
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for PRKCI gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
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| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for PRKCI | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PRKCI | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PRKCI | | | OriGene Protein Over-expression Lysate for PRKCI | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for PRKCI | | OriGene 3'-UTR Clone for PRKCI | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PRKCI | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PRKCI | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for PRKCI | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat PRKCI | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PRKCI | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PRKCI | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PRKCI | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PRKCI | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PRKCI |
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| | | | Tocris compounds for PRKCI |
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|  |  |  | | | | ThermoFisher Antibodies for PRKCI |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKCI |
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