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Aliases for PRKCH Gene

Aliases for PRKCH Gene

  • Protein Kinase C Eta 2 3 5
  • EC 2.7.11.13 4 61
  • NPKC-Eta 3 4
  • PRKCL 3 4
  • PKC-L 3 4
  • PKCL 3 4
  • Protein Kinase C Eta Type 3
  • Protein Kinase C, Eta 2
  • EC 2.7.11 61

External Ids for PRKCH Gene

Previous HGNC Symbols for PRKCH Gene

  • PRKCL

Previous GeneCards Identifiers for PRKCH Gene

  • GC14P059114
  • GC14P055582
  • GC14P059778
  • GC14P060858
  • GC14P061788
  • GC14P041951
  • GC14P061655

Summaries for PRKCH Gene

Entrez Gene Summary for PRKCH Gene

  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]

GeneCards Summary for PRKCH Gene

PRKCH (Protein Kinase C Eta) is a Protein Coding gene. Diseases associated with PRKCH include Stroke, Ischemic and Glioblastoma. Among its related pathways are VEGF Signaling Pathway and Signaling by GPCR. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKCE.

UniProtKB/Swiss-Prot for PRKCH Gene

  • Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Tocris Summary for PRKCH Gene

  • Protein kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanism. Conventional PKCs (cPKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol.

Gene Wiki entry for PRKCH Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKCH Gene

Genomics for PRKCH Gene

Regulatory Elements for PRKCH Gene

Enhancers for PRKCH Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH14F061320 1.5 FANTOM5 Ensembl ENCODE 33.6 +149.9 149854 33.6 HDGF PKNOX1 WRNIP1 SIN3A YBX1 FEZF1 ZNF2 ZNF143 FOS SP3 PRKCH TRMT5 TMEM30B LOC105370527 ENSG00000247287 GC14M061347
GH14F061498 1.4 FANTOM5 Ensembl ENCODE 18.6 +315.1 315108 8.9 HDGF TBP PKNOX1 TBL1XR1 RAD21 ZNF121 FOS CEBPB JUNB REST PRKCH FLJ22447 LOC101927780 SNAPC1 HIF1A GC14M061347 ENSG00000258989
GH14F061300 0.2 ENCODE 13.1 +113.3 113309 0.0 PRKCH GC14P061300 ENSG00000247287
GH14F061299 0.8 Ensembl ENCODE 12.8 +111.7 111742 0.6 GATA3 IKZF1 ZNF600 EBF1 ZBTB17 ENSG00000247287 PRKCH GC14P061300 TMEM30B
GH14F061376 1 FANTOM5 Ensembl ENCODE 12.3 +197.6 197612 17.9 HDGF PKNOX1 WRNIP1 FEZF1 YBX1 ZNF2 ZNF121 ZNF143 ZNF207 FOS PRKCH TMEM30B SALRNA1 LOC105370525 ENSG00000247287 GC14M061347
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around PRKCH on UCSC Golden Path with GeneCards custom track

Genomic Location for PRKCH Gene

Chromosome:
14
Start:
61,187,559 bp from pter
End:
61,550,980 bp from pter
Size:
363,422 bases
Orientation:
Plus strand

Genomic View for PRKCH Gene

Genes around PRKCH on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRKCH Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRKCH Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKCH Gene

Proteins for PRKCH Gene

  • Protein details for PRKCH Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P24723-KPCL_HUMAN
    Recommended name:
    Protein kinase C eta type
    Protein Accession:
    P24723
    Secondary Accessions:
    • B4DJN5
    • Q16246
    • Q8NE03

    Protein attributes for PRKCH Gene

    Size:
    683 amino acids
    Molecular mass:
    77828 Da
    Quaternary structure:
    • Interacts with FYN and RALA (By similarity). Interacts with DGKQ.

    Three dimensional structures from OCA and Proteopedia for PRKCH Gene

    Alternative splice isoforms for PRKCH Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PRKCH Gene

Selected DME Specific Peptides for PRKCH Gene

P24723:
  • VNGGDLM
  • GIIYRDLK
  • VLGKGSFGKV
  • GKGSFGKV
  • NVAPNCGV
  • GVLLYEM
  • DFGMCKE
  • QDDDVECTM
  • KEEPVLTP
  • YRDLKLDN
  • RQGLQCK
  • DRLFFVME
  • CTMTEKR
  • TFCDHCGSLL
  • YAVKVLKKDVILQDDDV
  • CSHCREFIWG
  • GTPDYIAPE
  • NEDDLFEAILND
  • TFCGTPDYIAPEI
  • NFDPDFIK
  • YIAPEIL
  • HPFFKEIDW
  • VVHKRCH
  • NGHKFMATYLRQPTYCSHCR
  • RDLKLDN

Post-translational modifications for PRKCH Gene

  • Ubiquitination at Lys 36 and Lys 404
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PRKCH (PKCH)
  • Abcam antibodies for PRKCH
  • Cloud-Clone Corp. Antibodies for PRKCH
  • Santa Cruz Biotechnology (SCBT) Antibodies for PRKCH

Domains & Families for PRKCH Gene

Graphical View of Domain Structure for InterPro Entry

P24723

UniProtKB/Swiss-Prot:

KPCL_HUMAN :
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
Domain:
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Contains 1 AGC-kinase C-terminal domain.
  • Contains 1 C2 domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Similarity:
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
genes like me logo Genes that share domains with PRKCH: view

Function for PRKCH Gene

Molecular function for PRKCH Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation.
UniProtKB/Swiss-Prot Function:
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Enzyme Numbers (IUBMB) for PRKCH Gene

Gene Ontology (GO) - Molecular Function for PRKCH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity IEA --
GO:0004697 protein kinase C activity IEA,TAS 1986216
GO:0004699 calcium-independent protein kinase C activity IEA --
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with PRKCH: view
genes like me logo Genes that share phenotypes with PRKCH: view

Animal Models for PRKCH Gene

MGI Knock Outs for PRKCH:

Animal Model Products

  • Taconic Biosciences Mouse Models for PRKCH

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for PRKCH

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PRKCH Gene

Localization for PRKCH Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRKCH Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PRKCH Gene COMPARTMENTS Subcellular localization image for PRKCH gene
Compartment Confidence
cytosol 5
extracellular 5
plasma membrane 5
mitochondrion 2
cytoskeleton 1
nucleus 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for PRKCH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IEA --
GO:0005737 cytoplasm IEA,IDA 15632189
GO:0005829 cytosol ISS,TAS --
GO:0005886 plasma membrane IDA 15632189
GO:0005911 cell-cell junction IEA --
genes like me logo Genes that share ontologies with PRKCH: view

Pathways & Interactions for PRKCH Gene

genes like me logo Genes that share pathways with PRKCH: view

Pathways by source for PRKCH Gene

SIGNOR curated interactions for PRKCH Gene

Activates:
Inactivates:

Gene Ontology (GO) - Biological Process for PRKCH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IEA,IMP 22304920
GO:0007165 signal transduction TAS 1986216
GO:0010744 positive regulation of macrophage derived foam cell differentiation ISS --
GO:0016310 phosphorylation IEA --
GO:0018105 peptidyl-serine phosphorylation IBA --
genes like me logo Genes that share ontologies with PRKCH: view

Drugs & Compounds for PRKCH Gene

(19) Drugs for PRKCH Gene - From: ApexBio, DGIdb, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Ingenol Mebutate Approved Pharma 0
Adenosine triphosphate Approved Nutra 0
Staurosporine Experimental Pharma Positive, Allosteric regulator Protein kinase inhibitor,potent and cell permeable 45
(-)-Epigallocatechin gallate (EGCG) Pharma Antioxidant, antiangiogenic and antitumor agent 0
Ro 31-8220 Mesylate Pharma Pan-PKC inhibitor 0

(5) Additional Compounds for PRKCH Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group C
  • 1-Myristoyl-3-stearoyl-sn-glycerol
  • 1-Tetradecanoyl-3-octadecanoyl-sn-glycerol
  • DAG(14:0/0:0/18:0)
  • DAG(32:0)
  • DG(32:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)

(5) Tocris Compounds for PRKCH Gene

Compound Action Cas Number
Enzastaurin Potent PKCbeta inhibitor 170364-57-5
GF 109203X Protein kinase C inhibitor 133052-90-1
Go 6976 Potent protein kinase C inhibitor; selective for alpha and beta isozymes 136194-77-9
Go 6983 Broad spectrum PKC inhibitor 133053-19-7
Phorbol 12-myristate 13-acetate Protein kinase C activator 16561-29-8

(4) ApexBio Compounds for PRKCH Gene

Compound Action Cas Number
(-)-Epigallocatechin gallate (EGCG) Antioxidant, antiangiogenic and antitumor agent 989-51-5
Ro 31-8220 Mesylate Pan-PKC inhibitor 138489-18-6
Sotrastaurin (AEB071) PKC inhibitor 425637-18-9
Staurosporine Protein kinase inhibitor,potent and cell permeable 62996-74-1
genes like me logo Genes that share compounds with PRKCH: view

Drug Products

Transcripts for PRKCH Gene

Unigene Clusters for PRKCH Gene

Protein kinase C, eta:
Representative Sequences:

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for PRKCH

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PRKCH Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19a · 19b
SP1: - - - - - - -
SP2: - -
SP3:
SP4: -
SP5:
SP6: - - - -
SP7: - - -
SP8:

Relevant External Links for PRKCH Gene

GeneLoc Exon Structure for
PRKCH
ECgene alternative splicing isoforms for
PRKCH

Expression for PRKCH Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PRKCH Gene

mRNA differential expression in normal tissues according to GTEx for PRKCH Gene

This gene is overexpressed in Whole Blood (x4.4).

Protein differential expression in normal tissues from HIPED for PRKCH Gene

This gene is overexpressed in CD8 Tcells (22.4), Tlymphocyte (13.7), Lung (12.1), and Placenta (6.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PRKCH Gene



Protein tissue co-expression partners for PRKCH Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of PRKCH Gene:

PRKCH

SOURCE GeneReport for Unigene cluster for PRKCH Gene:

Hs.333907

mRNA Expression by UniProt/SwissProt for PRKCH Gene:

P24723-KPCL_HUMAN
Tissue specificity: Most abundant in lung, less in heart and skin.
genes like me logo Genes that share expression patterns with PRKCH: view

Primer Products

Orthologs for PRKCH Gene

This gene was present in the common ancestor of animals.

Orthologs for PRKCH Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PRKCH 34 35
  • 99.61 (n)
oppossum
(Monodelphis domestica)
Mammalia PRKCH 35
  • 94 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PRKCH 34 35
  • 93.07 (n)
cow
(Bos Taurus)
Mammalia PRKCH 34 35
  • 92.42 (n)
rat
(Rattus norvegicus)
Mammalia Prkch 34
  • 90.09 (n)
mouse
(Mus musculus)
Mammalia Prkch 34 16 35
  • 90.03 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia PRKCH 35
  • 80 (a)
OneToOne
chicken
(Gallus gallus)
Aves PRKCH 34 35
  • 79.31 (n)
lizard
(Anolis carolinensis)
Reptilia PRKCH 35
  • 86 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia prkch 34
  • 72.1 (n)
zebrafish
(Danio rerio)
Actinopterygii prkcha 34 35
  • 66.22 (n)
prkchb 35
  • 62 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Pkc98E 36 35
  • 53 (a)
Pkcdgr 36
  • 47 (a)
worm
(Caenorhabditis elegans)
Secernentea pkc-1 35
  • 51 (a)
OneToMany
R12G8.1 36
  • 46 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.2703 35
  • 48 (a)
OneToMany
Species where no ortholog for PRKCH was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PRKCH Gene

ENSEMBL:
Gene Tree for PRKCH (if available)
TreeFam:
Gene Tree for PRKCH (if available)

Paralogs for PRKCH Gene

Paralogs for PRKCH Gene

Pseudogenes.org Pseudogenes for PRKCH Gene

genes like me logo Genes that share paralogs with PRKCH: view

Variants for PRKCH Gene

Sequence variations from dbSNP and Humsavar for PRKCH Gene

SNP ID Clin