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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

PRKCB Gene

protein-coding GIFtS: 68
GCID: GC16P023847

protein kinase C, beta

(Previous names: protein kinase C, beta 1 )
(Previous symbols: PRKCB2, PKCB, PRKCB1)

Explore 88 diseases affiliated with
PRKCB via

MalaCards

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Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Protein Kinase C, Beta¹ ²		EC 2.7.11.13³ ⁸
PKCB¹ ² ³ ⁵		Protein Kinase C, Beta 1¹
PRKCB1¹ ² ³ ⁵		Protein Kinase C Beta Type²
PRKCB2¹ ²		Protein Kinase C, Beta 1 Polypeptide²
PKC-B² ³		EC 2.7.11⁸
PKC-Beta¹

External Ids:	HGNC: 9395¹	Entrez Gene: 5579²	Ensembl: ENSG00000166501⁷	OMIM: 176970⁵	UniProtKB: P05771³

Export aliases for PRKCB gene to outside databases

Previous GC identifers: GC16P023755 GC16P021938

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for PRKCB:

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium

and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known

to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol

esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to

play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase

has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction,

endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may

also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced

transcript variants encoding distinct isoforms have been reported. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: KPCB_HUMAN, P05771

Function: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved

in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced

apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells

proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the

activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559',

'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the

BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and

activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating

BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane

localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of

oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial

accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen

receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating

phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that

prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of

IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May

participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated

translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably

involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells,

activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits

PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also

involved in triglyceride homeostasis (By similarity)

Gene Wiki entry for PRKCB (PRKCB1)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000016.9 NC_018927.1 NT_010393.16

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the PRKCB gene promoter:
AML1a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: PRKCB promoter sequence

Search SABiosciences Chromatin IP Primers for PRKCB

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PRKCB

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16p11.2 Ensembl cytogenetic band: 16p12.2 HGNC cytogenetic band: 16p12

PRKCB Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PRKCB gene location

GeneLoc information about chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16P023847: view genomic region (about GC identifiers)

Start:	23,847,300 bp from pter	End:	24,231,932 bp from pter
Size:	384,633 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: KPCB_HUMAN, P05771 (See protein sequence)

Recommended Name: Protein kinase C beta type

Size: 671 amino acids; 76869 Da

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity)

Subunit: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both

proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR

Subcellular location: Cytoplasm (By similarity). Nucleus. Membrane; Peripheral membrane protein (By similarity)

1 PDB 3D structure from and Proteopedia for PRKCB:

2I0E (3D)

Secondary accessions: C5IFJ8 D3DWF5 O43744 P05127 Q15138 Q93060 Q9UE49 Q9UE50 Q9UEH8 Q9UJ30 Q9UJ33

Alternative splicing: 2 isoforms: P05771-1 P05771-2 (Contains a phosphothreonine at position 641. Contains a phosphoserine at position 660)

Explore the universe of human proteins at neXtProt for PRKCB: NX_P05771

Post-translational modifications:

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent

autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release

the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no

effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the

activation of MAPK/ERK signaling cascade (By similarity)¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P05771

4/50 DME Specific Peptides for PRKCB (P05771) (see all 50)

VNGGDLM

VRDAKNL

FYAAEIA

WGFGKQG

PRKCB Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_002729.2 NP_997700.1

ENSEMBL proteins:

ENSP00000318315 ENSP00000305355 ENSP00000454428 ENSP00000459227 ENSP00000457980

ENSP00000458564

Reactome Protein details: P05771
Human Recombinant Protein Products:

	EMD Millipore Purified and/or Recombinant PRKCB Protein
	Browse R&D Systems for human recombinant proteins
	Enzo Life Sciences proteins for PRKCB
	OriGene Purified Proteins (see all 2): PRKCB OriGene Protein Over-expression Lysate (see all 3): PRKCB OriGene Custom Protein Services for PRKCB
	GenScript Custom Purified and Recombinant Proteins Services for PRKCB
	Novus Biologicals PRKCB Proteins Novus Biologicals PRKCB Lysates
	Browse Sino Biological Recombinant Proteins
	ProSpec Recombinant Protein for PRKCB
	Browse Proteins at Uscn

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005737	cytoplasm	IDA	15632189
GO:0005829	cytosol	TAS	--
GO:0005886	plasma membrane	TAS	--

PRKCB for ontologies About GeneDecksing

PRKCB Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of PRKCB
	R&D Systems Antibodies for PRKCB (PKC beta 2, PKC beta 1)
	Cell Signaling Technology (CST) Antibodies for PRKCB (PKCB iso2, PKCB)
	OriGene Antibodies (see all 3): PRKCB OriGene Custom Antibody Services for PRKCB
	GenScript Superior Antibodies for PRKCB
	Novus Biologicals PRKCB Antibodies
	Abcam antibodies for PRKCB
	Browse Antibodies at Uscn
	ThermoFisher Antibody for PRKCB

Assay Products for PRKCB:

	EMD Millipore Kits and Assays for the Analysis of PRKCB
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript PRKCB-Activity-based Kinase Assay for Compound Screening
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs and CLIAs at Uscn

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

PRKCB for domains About GeneDecksing

5/14 InterPro domains/families (see all 14):

IPR017441 Protein_kinase_ATP_BS

IPR002290 Ser/Thr_dual-sp_kinase_dom

IPR020454 DAG/PE-bd

IPR011009 Kinase-like_dom

IPR017892 Pkinase_C

Graphical View of Domain Structure for InterPro Entry P05771

ProtoNet protein and cluster: P05771

3 Blocks protein families:

IPB000008 C2 domain

IPB000961 Protein kinase C-terminal domain

IPB002219 Protein kinase C

UniProtKB/Swiss-Prot: KPCB_HUMAN, P05771

Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily

Similarity: Contains 1 AGC-kinase C-terminal domain

Similarity: Contains 1 C2 domain

Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers

Similarity: Contains 1 protein kinase domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary: