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Aliases for PRKAG3 Gene

Aliases for PRKAG3 Gene

  • Protein Kinase AMP-Activated Non-Catalytic Subunit Gamma 3 2 3
  • Protein Kinase, AMP-Activated, Gamma 3 Non-Catalytic Subunit 2 3 5
  • AMPK Subunit Gamma-3 3 4
  • AMPK Gamma3 3 4
  • AMPKG3 3 4
  • 5-AMP-Activated Protein Kinase, Gamma-3 Subunit 3
  • AMPK Gamma-3 Chain 3

External Ids for PRKAG3 Gene

Previous GeneCards Identifiers for PRKAG3 Gene

  • GC02M217702
  • GC02M218408
  • GC02M219651
  • GC02M219889
  • GC02M219512
  • GC02M219395
  • GC02M211540

Summaries for PRKAG3 Gene

Entrez Gene Summary for PRKAG3 Gene

  • The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKAG3 Gene

PRKAG3 (Protein Kinase AMP-Activated Non-Catalytic Subunit Gamma 3) is a Protein Coding gene. Diseases associated with PRKAG3 include wolff-parkinson-white syndrome. Among its related pathways are Gene Expression and Signaling by GPCR. GO annotations related to this gene include protein kinase binding and adenyl nucleotide binding. An important paralog of this gene is PRKAG2.

UniProtKB/Swiss-Prot for PRKAG3 Gene

  • AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene Wiki entry for PRKAG3 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKAG3 Gene

Genomics for PRKAG3 Gene

Regulatory Elements for PRKAG3 Gene

Promoters for PRKAG3 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around PRKAG3 on UCSC Golden Path with GeneCards custom track

Genomic Location for PRKAG3 Gene

Chromosome:
2
Start:
218,822,383 bp from pter
End:
218,832,086 bp from pter
Size:
9,704 bases
Orientation:
Minus strand

Genomic View for PRKAG3 Gene

Genes around PRKAG3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRKAG3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRKAG3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKAG3 Gene

Proteins for PRKAG3 Gene

  • Protein details for PRKAG3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UGI9-AAKG3_HUMAN
    Recommended name:
    5-AMP-activated protein kinase subunit gamma-3
    Protein Accession:
    Q9UGI9
    Secondary Accessions:
    • Q4QQG8
    • Q4V779
    • Q9NRL1

    Protein attributes for PRKAG3 Gene

    Size:
    489 amino acids
    Molecular mass:
    54258 Da
    Quaternary structure:
    • AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2 (By similarity).

    Alternative splice isoforms for PRKAG3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PRKAG3 Gene

Proteomics data for PRKAG3 Gene at MOPED

Post-translational modifications for PRKAG3 Gene

  • Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for PRKAG3 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PRKAG3 (AMPK gamma 3)

No data available for DME Specific Peptides for PRKAG3 Gene

Domains & Families for PRKAG3 Gene

Protein Domains for PRKAG3 Gene

InterPro:
Blocks:
ProtoNet:

Graphical View of Domain Structure for InterPro Entry

Q9UGI9

UniProtKB/Swiss-Prot:

AAKG3_HUMAN :
  • The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
  • Belongs to the 5-AMP-activated protein kinase gamma subunit family.
Domain:
  • The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
  • The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
  • Contains 4 CBS domains.
Family:
  • Belongs to the 5-AMP-activated protein kinase gamma subunit family.
genes like me logo Genes that share domains with PRKAG3: view

Function for PRKAG3 Gene

Molecular function for PRKAG3 Gene

UniProtKB/Swiss-Prot Function:
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene Ontology (GO) - Molecular Function for PRKAG3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004679 AMP-activated protein kinase activity IEA --
GO:0019901 protein kinase binding IDA 10698692
genes like me logo Genes that share ontologies with PRKAG3: view
genes like me logo Genes that share phenotypes with PRKAG3: view

Animal Models for PRKAG3 Gene

MGI Knock Outs for PRKAG3:

Animal Model Products

miRNA for PRKAG3 Gene

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PRKAG3 Gene

Localization for PRKAG3 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PRKAG3 Gene COMPARTMENTS Subcellular localization image for PRKAG3 gene
Compartment Confidence
cytosol 5
extracellular 5
plasma membrane 3
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for PRKAG3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0031588 nucleotide-activated protein kinase complex IEA --
genes like me logo Genes that share ontologies with PRKAG3: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for PRKAG3 Gene

Pathways & Interactions for PRKAG3 Gene

genes like me logo Genes that share pathways with PRKAG3: view

Pathways by source for PRKAG3 Gene

SIGNOR curated interactions for PRKAG3 Gene

Is inactivated by:

Gene Ontology (GO) - Biological Process for PRKAG3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0006468 protein phosphorylation IEA --
GO:0006996 organelle organization TAS --
GO:0007005 mitochondrion organization TAS --
GO:0007050 cell cycle arrest TAS --
genes like me logo Genes that share ontologies with PRKAG3: view

Drugs & Compounds for PRKAG3 Gene

(4) Drugs for PRKAG3 Gene - From: ApexBio and DrugBank

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Aspirin Approved Pharma Channel blocker, Target, activator 1029
AICAR Experimental Pharma AMPK activator 0
Dorsomorphin 2HCl Pharma AMPK inhibitor 0
Phenformin HCl Pharma 0

(3) ApexBio Compounds for PRKAG3 Gene

Compound Action Cas Number
AICAR AMPK activator 2627-69-2
Dorsomorphin 2HCl AMPK inhibitor 1219168-18-9
Phenformin HCl 834-28-6
genes like me logo Genes that share compounds with PRKAG3: view

Transcripts for PRKAG3 Gene

mRNA/cDNA for PRKAG3 Gene

(1) REFSEQ mRNAs :
(8) Additional mRNA sequences :
(10) Selected AceView cDNA sequences:
(6) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for PRKAG3 Gene

Protein kinase, AMP-activated, gamma 3 non-catalytic subunit:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for PRKAG3 Gene

No ASD Table

Relevant External Links for PRKAG3 Gene

GeneLoc Exon Structure for
PRKAG3
ECgene alternative splicing isoforms for
PRKAG3

Expression for PRKAG3 Gene

mRNA expression in normal human tissues for PRKAG3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PRKAG3 Gene

This gene is overexpressed in Muscle - Skeletal (x47.0).

SOURCE GeneReport for Unigene cluster for PRKAG3 Gene Hs.591634

mRNA Expression by UniProt/SwissProt for PRKAG3 Gene

Q9UGI9-AAKG3_HUMAN
Tissue specificity: Skeletal muscle, with weak expression in heart and pancreas.
genes like me logo Genes that share expression patterns with PRKAG3: view

Primer Products

In Situ Assay Products

No data available for Protein differential expression in normal tissues , Protein expression and Protein tissue co-expression partners for PRKAG3 Gene

Orthologs for PRKAG3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PRKAG3 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia PRKAG3 35
  • 87.43 (n)
  • 85.45 (a)
PRKAG3 36
  • 84 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PRKAG3 35
  • 86.24 (n)
  • 83.82 (a)
PRKAG3 36
  • 79 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Prkag3 35
  • 85.31 (n)
  • 85.04 (a)
Prkag3 16
Prkag3 36
  • 85 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia PRKAG3 35
  • 99.32 (n)
  • 98.98 (a)
PRKAG3 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Prkag3 35
  • 83.81 (n)
  • 84.02 (a)
oppossum
(Monodelphis domestica)
Mammalia PRKAG3 36
  • 71 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PRKAG3 36
  • 79 (a)
OneToOne
chicken
(Gallus gallus)
Aves PRKAG3 35
  • 75.6 (n)
  • 75.71 (a)
PRKAG3 36
  • 67 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PRKAG3 36
  • 71 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia prkag3 35
  • 70.04 (n)
  • 73.73 (a)
African clawed frog
(Xenopus laevis)
Amphibia prkag3-prov 35
zebrafish
(Danio rerio)
Actinopterygii prkag3b 35
  • 64.74 (n)
  • 67.95 (a)
prkag3a 36
  • 62 (a)
OneToMany
prkag3b 36
  • 64 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta SNF4Agamma 37
  • 53 (a)
SNF4Agamma 36
  • 15 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea T20F7.6 37
  • 37 (a)
Y111B2A.8 37
  • 49 (a)
Y41G9A.3 37
  • 32 (a)
aakg-1 36
  • 30 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SNF4 36
  • 34 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7193 36
  • 45 (a)
OneToMany
Species with no ortholog for PRKAG3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PRKAG3 Gene

ENSEMBL:
Gene Tree for PRKAG3 (if available)
TreeFam:
Gene Tree for PRKAG3 (if available)

Paralogs for PRKAG3 Gene

Paralogs for PRKAG3 Gene

(2) SIMAP similar genes for PRKAG3 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with PRKAG3: view

Variants for PRKAG3 Gene

Polymorphic Variants from UniProtKB/Swiss-Prot for PRKAG3 Gene

Q9UGI9-AAKG3_HUMAN
PRKAG3 genetic variants can be associated with increased glycogen content in skeletal muscle [MIM:604976]. Muscle fibers from carriers of variant Trp-225 have approximately 90% more muscle glycogen content than controls and decreased levels of intramuscular triglyceride.

Sequence variations from dbSNP and Humsavar for PRKAG3 Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type
rs692243 - 218,830,764(-) GGGAG(C/G)CACCA reference, missense
rs35050588 - 218,830,154(+) TTCCA(C/G/T)CAGGC reference, missense
rs33985460 - 218,827,078(+) GGGCC(A/G)GGGCA reference, missense
VAR_069470 -
VAR_069471 -

Structural Variations from Database of Genomic Variants (DGV) for PRKAG3 Gene

Variant ID Type Subtype PubMed ID
nsv528407 CNV Loss 19592680

Variation tolerance for PRKAG3 Gene

Residual Variation Intolerance Score: 44.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.63; 78.25% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PRKAG3 Gene

HapMap Linkage Disequilibrium report
PRKAG3
Human Gene Mutation Database (HGMD)
PRKAG3

Disorders for PRKAG3 Gene

MalaCards: The human disease database

(1) MalaCards diseases for PRKAG3 Gene - From: DISEASES

Disorder Aliases PubMed IDs
wolff-parkinson-white syndrome
  • anomalous a-v excitation
- elite association - COSMIC cancer census association via MalaCards

Relevant External Links for PRKAG3

Genetic Association Database (GAD)
PRKAG3
Human Genome Epidemiology (HuGE) Navigator
PRKAG3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PRKAG3
genes like me logo Genes that share disorders with PRKAG3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PRKAG3 Gene

Publications for PRKAG3 Gene

  1. A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle. (PMID: 10818001) Milan D. … Andersson L. (Science 2000) 2 3 4 67
  2. Role of AMP-activated protein kinase gamma 3 genetic variability in glucose and lipid metabolism in non-diabetic whites. (PMID: 17701023) Weyrich P. … HAoring H.U. (Diabetologia 2007) 3 23
  3. Gain-of-function R225W mutation in human AMPKgamma(3) causing increased glycogen and decreased triglyceride in skeletal muscle. (PMID: 17878938) Costford S.R. … Harper M.E. (PLoS ONE 2007) 3 23
  4. Comparative sequence analysis of the PRKAG3 region between human and pig: evolution of repetitive sequences and potential new exons. (PMID: 14970697) Amarger V. … Andersson L. (Cytogenet. Genome Res. 2003) 3 23
  5. Systematic screening reveals a role for BRCA1 in the response to transcription-associated DNA damage. (PMID: 25184681) Hill S.J. … Livingston D.M. (Genes Dev. 2014) 3

Products for PRKAG3 Gene

Sources for PRKAG3 Gene

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