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Aliases for PRKAG1 Gene

Aliases for PRKAG1 Gene

  • Protein Kinase AMP-Activated Non-Catalytic Subunit Gamma 1 2 3
  • Protein Kinase, AMP-Activated, Gamma 1 Non-Catalytic Subunit 2 3 5
  • AMPK Gamma1 3 4
  • AMPKG 3 4
  • 5-AMP-Activated Protein Kinase, Gamma-1 Subunit 3
  • AMPK Subunit Gamma-1 4
  • AMPK Gamma-1 Chain 3

External Ids for PRKAG1 Gene

Previous GeneCards Identifiers for PRKAG1 Gene

  • GC12P049402
  • GC12M049112
  • GC12M047682
  • GC12M049396
  • GC12M046427

Summaries for PRKAG1 Gene

Entrez Gene Summary for PRKAG1 Gene

  • The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKAG1 Gene

PRKAG1 (Protein Kinase AMP-Activated Non-Catalytic Subunit Gamma 1) is a Protein Coding gene. Diseases associated with PRKAG1 include Ascariasis. Among its related pathways are Glucose / Energy Metabolism and ERK Signaling. GO annotations related to this gene include protein kinase activity and ADP binding. An important paralog of this gene is PRKAG3.

UniProtKB/Swiss-Prot for PRKAG1 Gene

  • AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene Wiki entry for PRKAG1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKAG1 Gene

Genomics for PRKAG1 Gene

Regulatory Elements for PRKAG1 Gene

Enhancers for PRKAG1 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around PRKAG1 on UCSC Golden Path with GeneCards custom track

Promoters for PRKAG1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around PRKAG1 on UCSC Golden Path with GeneCards custom track

Genomic Location for PRKAG1 Gene

Chromosome:
12
Start:
49,002,272 bp from pter
End:
49,019,197 bp from pter
Size:
16,926 bases
Orientation:
Minus strand

Genomic View for PRKAG1 Gene

Genes around PRKAG1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRKAG1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRKAG1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKAG1 Gene

Proteins for PRKAG1 Gene

  • Protein details for PRKAG1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P54619-AAKG1_HUMAN
    Recommended name:
    5-AMP-activated protein kinase subunit gamma-1
    Protein Accession:
    P54619
    Secondary Accessions:
    • B4DDT7
    • Q8N7V9

    Protein attributes for PRKAG1 Gene

    Size:
    331 amino acids
    Molecular mass:
    37579 Da
    Quaternary structure:
    • AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

    Three dimensional structures from OCA and Proteopedia for PRKAG1 Gene

    Alternative splice isoforms for PRKAG1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PRKAG1 Gene

Proteomics data for PRKAG1 Gene at MOPED

Post-translational modifications for PRKAG1 Gene

  • Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
  • Ubiquitination at Lys 170
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PRKAG1 (AMPK gamma 1)
  • Cloud-Clone Corp. Antibodies for PRKAG1

No data available for DME Specific Peptides for PRKAG1 Gene

Domains & Families for PRKAG1 Gene

Protein Domains for PRKAG1 Gene

InterPro:
Blocks:
ProtoNet:

Graphical View of Domain Structure for InterPro Entry

P54619

UniProtKB/Swiss-Prot:

AAKG1_HUMAN :
  • The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
  • Belongs to the 5-AMP-activated protein kinase gamma subunit family.
Domain:
  • The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
  • The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
  • Contains 4 CBS domains.
Family:
  • Belongs to the 5-AMP-activated protein kinase gamma subunit family.
genes like me logo Genes that share domains with PRKAG1: view

Function for PRKAG1 Gene

Molecular function for PRKAG1 Gene

GENATLAS Biochemistry:
protein kinase,cAMP activated ,gamma 1 non catalytic subunit,complexing with alpha 2 and beta 2 subunits,involved in fatty acid and cholesterol metabolism
UniProtKB/Swiss-Prot Function:
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene Ontology (GO) - Molecular Function for PRKAG1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004691 cAMP-dependent protein kinase activity TAS 9598317
GO:0005524 ATP binding ISS --
GO:0008603 cAMP-dependent protein kinase regulator activity TAS 8663446
GO:0016208 AMP binding ISS --
GO:0019901 protein kinase binding IDA 10698692
genes like me logo Genes that share ontologies with PRKAG1: view
genes like me logo Genes that share phenotypes with PRKAG1: view

Animal Models for PRKAG1 Gene

MGI Knock Outs for PRKAG1:

Animal Model Products

  • Taconic Biosciences Mouse Models for PRKAG1

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PRKAG1 Gene

Localization for PRKAG1 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PRKAG1 Gene COMPARTMENTS Subcellular localization image for PRKAG1 gene
Compartment Confidence
cytosol 5
extracellular 5
nucleus 3
cytoskeleton 1

Gene Ontology (GO) - Cellular Components for PRKAG1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with PRKAG1: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for PRKAG1 Gene

Pathways & Interactions for PRKAG1 Gene

genes like me logo Genes that share pathways with PRKAG1: view

SIGNOR curated interactions for PRKAG1 Gene

Activates:

Gene Ontology (GO) - Biological Process for PRKAG1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006110 regulation of glycolytic process TAS 8663446
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0006468 protein phosphorylation IDA 17028174
GO:0006996 organelle organization TAS --
GO:0007005 mitochondrion organization TAS --
genes like me logo Genes that share ontologies with PRKAG1: view

Drugs & Compounds for PRKAG1 Gene

(4) Drugs for PRKAG1 Gene - From: DrugBank and ApexBio

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Aspirin Approved Pharma Channel blocker, Target, activator 1059
AICAR Experimental Pharma AMPK activator 0
Dorsomorphin 2HCl Pharma AMPK inhibitor 0
Phenformin HCl Pharma 0

(3) ApexBio Compounds for PRKAG1 Gene

Compound Action Cas Number
AICAR AMPK activator 2627-69-2
Dorsomorphin 2HCl AMPK inhibitor 1219168-18-9
Phenformin HCl 834-28-6
genes like me logo Genes that share compounds with PRKAG1: view

Transcripts for PRKAG1 Gene

Unigene Clusters for PRKAG1 Gene

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for PRKAG1 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b · 7c ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^
SP1: - - - - - - - -
SP2: - - - - - - - - -
SP3: - - - - - - - -
SP4: - - - - - - -
SP5: - - -
SP6: - - - - - - - -
SP7: - -
SP8: - - - - - -
SP9: - - - -
SP10: -
SP11: - - -
SP12: - - - -

ExUns: 14 ^ 15 ^ 16a · 16b
SP1:
SP2:
SP3:
SP4:
SP5: -
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:

Relevant External Links for PRKAG1 Gene

GeneLoc Exon Structure for
PRKAG1
ECgene alternative splicing isoforms for
PRKAG1

Expression for PRKAG1 Gene

mRNA expression in normal human tissues for PRKAG1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for PRKAG1 Gene



SOURCE GeneReport for Unigene cluster for PRKAG1 Gene Hs.530862

genes like me logo Genes that share expression patterns with PRKAG1: view

Protein tissue co-expression partners for PRKAG1 Gene

- Elite partner

Primer Products

No data available for mRNA differential expression in normal tissues , Protein differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for PRKAG1 Gene

Orthologs for PRKAG1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for PRKAG1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia PRKAG1 35
  • 93.92 (n)
  • 98.78 (a)
PRKAG1 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PRKAG1 35
  • 94.02 (n)
  • 99.09 (a)
PRKAG1 36
  • 98 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Prkag1 35
  • 87.68 (n)
  • 97.58 (a)
Prkag1 16
Prkag1 36
  • 97 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia PRKAG1 35
  • 99.8 (n)
  • 99.41 (a)
PRKAG1 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Prkag1 35
  • 87.98 (n)
  • 96.67 (a)
oppossum
(Monodelphis domestica)
Mammalia PRKAG1 36
  • 88 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PRKAG1 36
  • 74 (a)
OneToOne
chicken
(Gallus gallus)
Aves PRKAG1 35
  • 77.94 (n)
  • 90.55 (a)
PRKAG1 36
  • 89 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PRKAG1 36
  • 86 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia prkag2 35
  • 74.27 (n)
  • 86.97 (a)
Str.2067 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.7123 35
zebrafish
(Danio rerio)
Actinopterygii prkag1 35
  • 73.29 (n)
  • 84.69 (a)
wufa94d09 35
prkag1 36
  • 78 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.10524 35
fruit fly
(Drosophila melanogaster)
Insecta SNF4Agamma 37
  • 63 (a)
SNF4Agamma 36
  • 15 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea T20F7.6 37
  • 34 (a)
Y111B2A.8 37
  • 55 (a)
Y41G9A.3 37
  • 31 (a)
aakg-1 35
  • 56.74 (n)
  • 55.02 (a)
aakg-1 36
  • 30 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SNF4 36
  • 34 (a)
OneToMany
rice
(Oryza sativa)
Liliopsida Os04g0401300 35
  • 42.46 (n)
  • 32.28 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7193 36
  • 49 (a)
OneToMany
Species with no ortholog for PRKAG1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PRKAG1 Gene

ENSEMBL:
Gene Tree for PRKAG1 (if available)
TreeFam:
Gene Tree for PRKAG1 (if available)

Paralogs for PRKAG1 Gene

Paralogs for PRKAG1 Gene

genes like me logo Genes that share paralogs with PRKAG1: view

Variants for PRKAG1 Gene

Sequence variations from dbSNP and Humsavar for PRKAG1 Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
VAR_033453 -
VAR_033454 -

Structural Variations from Database of Genomic Variants (DGV) for PRKAG1 Gene

Variant ID Type Subtype PubMed ID
nsv832404 CNV Gain 17160897
esv2662608 CNV Deletion 23128226

Variation tolerance for PRKAG1 Gene

Residual Variation Intolerance Score: 25.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.69; 32.30% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PRKAG1 Gene

Human Gene Mutation Database (HGMD)
PRKAG1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PRKAG1 Gene

Disorders for PRKAG1 Gene

MalaCards: The human disease database

(1) MalaCards diseases for PRKAG1 Gene - From: GeneCards

Disorder Aliases PubMed IDs
ascariasis
  • ascariasis - roundworm
- elite association - COSMIC cancer census association via MalaCards

Relevant External Links for PRKAG1

Genetic Association Database (GAD)
PRKAG1
Human Genome Epidemiology (HuGE) Navigator
PRKAG1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PRKAG1
genes like me logo Genes that share disorders with PRKAG1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PRKAG1 Gene

Publications for PRKAG1 Gene

  1. Non-catalytic beta- and gamma-subunit isoforms of the 5'-AMP- activated protein kinase. (PMID: 8621499) Gao G. … Witters L.A. (J. Biol. Chem. 1996) 2 3 4 67
  2. Mammalian AMP-activated protein kinase subfamily. (PMID: 8557660) Stapleton D. … Kemp B.E. (J. Biol. Chem. 1996) 2 3
  3. Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy. (PMID: 25852190) So J. … Colwill K. (Sci Signal 2015) 3
  4. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 3
  5. Prolyl isomerase Pin1 negatively regulates AMP-activated protein kinase (AMPK) by associating with the CBS domain in the I^ subunit. (PMID: 26276391) Nakatsu Y. … Asano T. (J. Biol. Chem. 2015) 3

Products for PRKAG1 Gene

Sources for PRKAG1 Gene

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