PRKAA1 Gene
protein-coding GIFtS: 64
GCID: GC05M040759
|
|
protein kinase, AMP-activated, alpha 1 catalytic subunit
| |
Aliases
for PRKAA1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Protein Kinase, AMP-Activated, Alpha 1 Catalytic Subunit1 2 | | 5'-AMP-Activated Protein Kinase, Catalytic Alpha-1 Chain2 | | AMPKa11 2 | | AMPK2 | | Acetyl-CoA Carboxylase Kinase2 3 | | AMP -Activate Kinase Alpha 1 Subunit2 | | Hydroxymethylglutaryl-CoA Reductase Kinase2 3 | | AMP-Activated Protein Kinase, Catalytic, Alpha-12 | | Tau-Protein Kinase PRKAA12 3 | | AMPK Alpha 12 | | ACACA Kinase2 3 | | AMPK13 | | AMPK Subunit Alpha-12 3 | | EC 2.7.11.263 | | HMGCR Kinase2 3 | | EC 2.7.11.273 | | EC 2.7.11.13 8 | | EC 2.7.11.313 | | 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-12 | | EC 2.7.118 |
Export aliases for PRKAA1 gene to outside databasesPrevious GC identifers: GC05M041069 GC05M041854 GC05M040804 GC05M040795 GC05M040711 |
Summaries
for PRKAA1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for PRKAA1:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. Thekinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates theactivities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that causeATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encodingdistinct isoforms have been observed. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: AAPK1_HUMAN, Q13131Function: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a keyrole in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activatesenergy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipidbiosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, andby longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarityby remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis byphosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fattyacid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitivelipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 andPFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporterSLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcriptionand chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2,FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a keyregulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in thecytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription.Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response tonutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complexand by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylatingand activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leadingto destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also hastau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading tophosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR,EEF2K, KLC1, NOS3 and SLC12A1
 summary
for PRKAA1: AMP-activated protein kinase (AMPK) is a heterodimeric protein serine/threonine kinase that is composed of
alpha- (catalytic) and beta/gamma- (regulatory) subunits. AMPK acts as a sensor of the energy status of
cells and ensures survival at times of metabolic stress. AMPK phosphorylates many metabolic enzymes to
stimulate catabolic pathways, such as ketogenesis, and inhibit anabolic pathways, such as protein synthesis.
The long-term activation of AMPK increases the capacity of cells to produce ATP. AMPK is regulated by
phosphorylation at the Thr-172 residue of the alpha-subunit by AMPKK and by phosphorylation by
calmodulin-dependent protein kinase kinase-beta (CamKKbeta). In addition, the ratio of AMP:ATP mediates
allosteric activation of the enzyme. AMPK is found throughout the body with high concentrations in
metabolically active tissues such as the skeletal muscles and liver.
Gene Wiki entry for PRKAA1 (Protein kinase, AMP-activated, alpha 1)
|
Genomic Views
for PRKAA1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000005.9 NC_018916.1 NT_006576.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the PRKAA1 gene promoter:
STAT1 AP-1 Egr-1 ATF-2 Nkx2-5 c-Jun STAT1alpha
Other transcription factors
Search SABiosciences Chromatin IP Primers for PRKAA1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PRKAA1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 5p12 Ensembl cytogenetic band: 5p13.1 HGNC cytogenetic band: 5p13.1PRKAA1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 5 GeneLoc Exon Structure GeneLoc location for GC05M040759: view genomic region
(about GC identifiers)
Start:
|
40,759,481 bp from pter |
End:
|
40,798,476 bp from pter |
Size:
|
38,996 bases |
Orientation:
|
minus strand |
|
Proteins
for PRKAA1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: AAPK1_HUMAN, Q13131 (See
protein sequence)Recommended Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1 Size: 559 amino acids; 64009 Da
Cofactor: Magnesium
Subunit: AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gammanon-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2
Subcellular location: Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters
Sequence caution: Sequence=AAA64850.1; Type=Erroneous initiation; Note=Translation N-terminally extended;Sequence=AAD43027.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH37303.1;Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAA36547.1; Type=Erroneous initiation;Note=Translation N-terminally extended; Sequence=BAG35788.1; Type=Erroneous initiation; Note=Translation N-terminallyextended;
Secondary accessions: A8MTQ6 B2R7E1 O00286 Q5D0E1 Q86VS1 Q9UNQ4Alternative splicing: 2 isoforms: Q13131-1 Q13131-2 Explore the universe of human proteins at neXtProt for PRKAA1: NX_Q13131
Post-translational modifications:
Ubiquitinated (By similarity)1
Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectablechanges in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated byprotein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPKactivity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q13131
4/32 DME Specific Peptides for PRKAA1 (Q13131) (see all 32)
PRKAA1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_006242.5 NP_996790.3
ENSEMBL proteins: ENSP00000380317 ENSP00000346148 ENSP00000296800 Reactome Protein details: Q13131
Human Recombinant Protein Products:
Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table
PRKAA1 for ontologies About GeneDecksing
PRKAA1 Antibody Products:
Assay Products for PRKAA1: |
Protein
Domains /
Families
for PRKAA1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
PRKAA1 for domains About GeneDecksing
5 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q13131ProtoNet protein and cluster: Q13131 UniProtKB/Swiss-Prot: AAPK1_HUMAN, Q13131Domain: The AIS (autoinhibitory sequence) region some sequence similarity with the ubiquitin-associated domains andrepresses kinase activitySimilarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamilySimilarity: Contains 1 protein kinase domain |
Function
for PRKAA1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: AAPK1_HUMAN, Q13131Function: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a keyrole in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activatesenergy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipidbiosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, andby longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarityby remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis byphosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fattyacid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitivelipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 andPFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporterSLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcriptionand chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2,FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a keyregulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in thecytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription.Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response tonutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complexand by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylatingand activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leadingto destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also hastau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading tophosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR,EEF2K, KLC1, NOS3 and SLC12A1Catalytic activity: ATP + a protein = ADP + a phosphoproteinCatalytic activity: ATP + [tau protein] = ADP + [tau protein] phosphateCatalytic activity: ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase(NADPH)] phosphateCatalytic activity: ATP + [acetyl-CoA carboxylase] = ADP + [acetyl-CoA carboxylase] phosphateEnzyme regulation: Activated by phosphorylation on Thr-183. Binding of AMP to non-catalytic gamma subunit (PRKAG1,PRKAG2 or PRKAG3) results in allosteric activation, inducing phosphorylation on Thr-183. AMP-binding to gamma subunitalso sustains activity by preventing dephosphorylation of Thr-183. ADP also stimulates Thr-183 phosphorylation,without stimulating already phosphorylated AMPK. ATP promotes dephosphorylation of Thr-183, rendering the enzymeinactive. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is muchmore abundant than AMP. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used toactivate AMPK in muscle and other cells in culture or ex vivo (PubMed:11602624). Selectively inhibited by compound C(6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, anatural polyphenol present in red wine, and S17834, a synthetic polyphenol Genatlas biochemistry entry for PRKAA1:protein kinase,cAMP activated,alpha 1 catalytic subunit,involved in fatty acid and cholesterol metabolism
Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112 EC 2.7.11.261 EC 2.7.11.271 EC 2.7.11.311
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PRKAA1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PRKAA1 (see all 5)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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Synthesis Service for PRKAA1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKAA1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKAA1 |
Gene Ontology (GO): 5/14 molecular function terms (GO ID links to tree view) (see all 14): About this table
PRKAA1 for ontologies About GeneDecksing
Animal Models:
Mouse knock-out Prkaa1tm1Vio for PRKAA1
6 MGI mutant phenotypes (inferred from 3 alleles ) (MGI details for Prkaa1):
PRKAA1 for phenotypes About GeneDecksing
|
Pathways & Interactions
for PRKAA1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/20 super-pathways (see all 20) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | IRS-related events | | | 2 | Development_Leptin signaling via PI3K-dependent pathway | | | 3 | Energy dependent regulation of mTOR by LKB1-AMPK | | | 4 | mTOR signalling | | | 5 | Development_CNTF receptor signaling | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1 EMD Millipore Pathway for PRKAA1
1  R&D Systems Pathway for PRKAA1
3 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for PRKAA1
1 
Cell Signaling Technology (CST) Pathway for PRKAA1
1 Tocris Bioscience Pathway for PRKAA1
3  GeneGo (Thomson Reuters) Pathways for PRKAA1
4  BioSystems Pathways for PRKAA1
5/14 
Reactome Pathways for PRKAA1 (see all 14)
1  PharmGKB Pathway for PRKAA1
5 
Kegg Pathways (Kegg details for PRKAA1):
PRKAA1 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for PRKAA1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/487 Interacting proteins for PRKAA1 (Q131311, 2, 3 ENSP000003461484) via UniProtKB, MINT, STRING, and/or I2D (see all 487)About this table
Gene Ontology (GO): 5/45 biological process terms (GO ID links to tree view) (see all 45): About this table
PRKAA1 for ontologies About GeneDecksing
|
Drugs & Compounds
for PRKAA1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
PRKAA1 for compounds About GeneDecksing
Compounds for PRKAA1 available from Tocris Bioscience About this table
10/13 HMDB Compounds for PRKAA1 (see all 13) About this table
3 DrugBank Compounds for PRKAA1 About this table
10/31 Novoseek chemical compound relationships for PRKAA1 gene (see all 31) About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| acadesine |
73.5 |
7 |
18798311 (3), 12417310 (1), 12145153 (1), 12208500 (1) |
| metformin |
72.2 |
65 |
14871885 (5), 18484962 (5), 12145153 (5), 18721796 (4) (see all 25) |
| atp |
68 |
107 |
18563357 (3), 17974626 (3), 16027121 (3), 17039396 (2) (see all 50) |
| fatty acid |
62.7 |
48 |
17997341 (3), 17500064 (2), 19245653 (2), 14641016 (2) (see all 28) |
| phenformin |
62.2 |
5 |
15919715 (4), 20444419 (1) |
| rapamycin |
60.6 |
7 |
18827172 (1), 19092695 (1), 18343152 (1), 19507259 (1) (see all 7) |
| glucose |
57.3 |
98 |
11467861 (4), 14532293 (4), 18563357 (3), 17974626 (3) (see all 52) |
| aicar |
57 |
6 |
15919715 (3), 16382178 (1), 11509501 (1) |
| acetyl-coa |
54.9 |
9 |
18297106 (2), 16868748 (1), 16990341 (1), 16434556 (1) (see all 7) |
| threonine |
48.5 |
17 |
9003365 (2), 19818314 (1), 15058305 (1), 11284715 (1) (see all 16) |
Search CenterWatch for drugs/clinical trials and news about PRKAA1 / AAPK1
|
Transcripts
for PRKAA1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for PRKAA1 gene (2 alternative transcripts): NM_006251.5 NM_206907.3 Unigene Cluster for PRKAA1: Protein kinase, AMP-activated, alpha 1 catalytic subunit Hs.43322 [show with all ESTs]Unigene Representative Sequence: NM_2069079 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000397128(uc003jmb.3 uc003jmc.3) ENST00000354209 ENST00000513152
ENST00000505783 ENST00000506652 ENST00000509874 ENST00000296800 ENST00000511248
ENST00000397006
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PRKAA1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PRKAA1 (see all 5)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): PRKAA1 (NM_206907) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PRKAA1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKAA1 |
Additional cDNA sequence: AB022017.1 AF100763.1 AK307546.1 AK312947.1 BC012622.1 BC037303.2 BC048980.1 U22456.1 Y12856.1 14 DOTS entries: DT.442118 DT.100688995 DT.100679863 DT.91884113 DT.120806759 DT.95236846 DT.120806707 DT.95167837 DT.422360 DT.120806740 DT.91760022 DT.120806706 DT.95167838 DT.120806753 24/205 AceView cDNA sequences (see all 205): AA382560 AA604847 AA358109 CB305609 BU520965 CB163807 BM977269 H88038 CD512594 AI698274 BQ720524 BM758818 BQ878361 AI681997 AL599370 AA320710 AU185712 AA889486 Y12856 AA902956 BQ267649 BG743416 CA413014 BX089121
GeneLoc Exon Structure
5/9 Alternative Splicing Database (ASD) splice patterns (SP) for PRKAA1 (see all 9) About this scheme
| ExUns: | 1a | · | 1b | ^ | 2a | · | 2b | · | 2c | ^ | 3a | · | 3b | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9a | · | 9b | · | 9c | · | 9d | ^ | 10 | ^ | 11 | ^ | 12a | · | 12b |
|---|
|
| SP1: | | | | | | | | | | | - | | - | | | | | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | - | | - | | | | - | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | - | | - | | - | | | | | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | - | | - | | | | - | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | - | | - | | - | | | | - | | | | | | - | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for PRKAA1
|
Expression
for PRKAA1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| PRKAA1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See PRKAA1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for PRKAA1
SOURCE GeneReport for Unigene cluster: Hs.43322
SABiosciences Expression via Pathway-Focused PCR Arrays including PRKAA1 (see all 8):
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PRKAA1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat PRKAA1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PRKAA1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PRKAA1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKAA1 |
Orthologs
for PRKAA1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for PRKAA1 gene from 8/31 species (see all 31) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
PRKAA11 |
protein kinase, AMP-activated, alpha 1 catalytic subunit |
85.01(n)
95.52(a) |
|
427185 NM_001039603.1 NP_001034692.1 |
lizard
(Anolis carolinensis) |
Reptilia |
PRKAA16 |
-- |
95(a) |
1 ↔ 1 |
2(6992269-7003150) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.52512 |
Xenopus laevis SNF1-like protein AMPK mRNA, complete more |
80.29(n) |
|
AF340021.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
prkaa12 |
protein kinase, AMP-activated, alpha 1 catalytic subunit |
78.11(n) |
|
368268 BI476044.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
SNF1A6 |
SNF1A/AMP-activated protein kinase |
57(a) |
1 → many |
X(1269580-1272890) |
worm
(Caenorhabditis elegans) |
Secernentea |
T01C8.1a3 |
ser/thr-protein kinase |
58(a)
(best of 5) |
|
X(16830550-16833650) -- |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
KIN101 |
SNF1-related protein kinase catalytic subunit alpha more |
57.92(n)
55.69(a) |
|
821259 NM_001125074.1 NP_001118546.1 |
rice
(Oryza sativa) |
Liliopsida |
Os.33932 |
Oryza sativa mRNA for OSK1, complete cds |
73.84(n) |
|
AK069613.1 |
ENSEMBL Gene Tree for PRKAA1 (if available)
TreeFam Gene Tree for PRKAA1 (if available) |
Paralogs
for PRKAA1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for PRKAA1 gene
- TSSK1B2 PRKAA22 HUNK2 TSSK62 TSSK32 MELK2 TSSK22 TSSK42
- STK112
18/181 SIMAP similar genes for PRKAA1 using alignment to 2 protein entries: AAPK1_HUMAN (see all proteins)
(see all similar genes):PRKAA2 MARK4 NUAK1 SIK2 BRSK1 SIK1
BRSK2 KIAA0999 SIK3 HUNK MARK1 MARK2
MELK DCLK1 DAPK3 PNCK DAPK1 SNRK
PRKAA1 for paralogs About GeneDecksing
|
Genomic Variants
for PRKAA1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 5 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for PRKAA1 (40759481 - 40798476 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for PRKAA1: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PRKAA1 |
|
Disorders
/ Diseases
for PRKAA1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
PRKAA1 for disorders About GeneDecksing
OMIM gene information: 602739
OMIM disorders: --
20/46  diseases for PRKAA1 (see all 46): About MalaCardswolff-parkinson-white syndrome type 2 diabetes mellitus tuberous sclerosis insulin resistance
fatty liver disease diabetes mellitus morbid obesity chronic lymphocytic leukemia
neurodegenerative disease lymphocytic leukemia insulinoma metabolic disorders
retinal degeneration liver disease cystic fibrosis squamous cell carcinoma
cardiomyopathy multiple sclerosis hepatitis c epididymitis
10/17 Novoseek disease relationships for PRKAA1 gene (see all 17) About this table
| Disease |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| obesity |
43.2 |
11 |
18719601 (2), 16508085 (1), 17438370 (1), 16644802 (1) (see all 8) |
| insulin sensitivity |
42.5 |
3 |
17485446 (1), 17869249 (1), 17530314 (1), 16434556 (1) |
| tuberous sclerosis |
41.3 |
1 |
19520853 (1) |
| insulin resistance |
37.9 |
6 |
15694373 (1), 17438370 (1), 19273380 (1), 18430992 (1) (see all 5) |
| insulinoma |
30.4 |
2 |
14871885 (1), 20206170 (1) |
| cardiac hypertrophy |
28.1 |
4 |
15759047 (2), 12397075 (1) |
| wolff-parkinson-white syndrome |
20.2 |
1 |
12397075 (1) |
| cancer |
18.8 |
23 |
19778642 (3), 19221486 (2), 19243571 (2), 19723334 (1) (see all 13) |
| cardiovascular diseases |
0 |
3 |
16597407 (1), 20060780 (1) |
| ischemia |
0 |
3 |
19359670 (1), 17556544 (1), 12960015 (1) |
Human Genome Epidemiology (HuGE) Navigator: PRKAA1 (14 documents)
Export disorders for PRKAA1 gene to outside databases
|
Publications
for PRKAA1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for PRKAA1 gene, integrated from 9 sources (see all 412):
(articles sorted by number of sources associating them with PRKAA1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Mammalian AMP-activated protein kinase subfamily. (PubMed id 8557660)1, 2, 3 Stapleton D.... Kemp B.E. (1996)
- TSC2 mediates cellular energy response to control cell growth and survival. (PubMed id 14651849)1, 2, 9 Inoki K....Guan K.L. (2003)
- AMPK phosphorylation of raptor mediates a metabolic checkpoint. (PubMed id 18439900)1, 2 Gwinn D.M....Shaw R.J. (2008)
- Identification and characterization of a novel folliculin-interacting protein FNIP2. (PubMed id 18403135)1, 2 Hasumi H.... Schmidt L.S. (2008)
- Conserved alpha-helix acts as autoinhibitory sequence in AMP-activated protein kinase alpha subunits. (PubMed id 17088252)1, 2 Pang T....Li J. (2007)
- Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved in AMPK and mTOR signaling. (PubMed id 17028174)1, 2 Baba M....Zbar B. (2006)
- Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase. (PubMed id 16054095)1, 2 Hawley S.A.... Hardie D.G. (2005)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. (PubMed id 14976552)1, 2 Lizcano J.M....Alessi D.R. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
|
External Searches for PRKAA1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing PRKAA1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing PRKAA1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PRKAA1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for PRKAA1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for PRKAA1 gene:
Search GeneIP for patents involving PRKAA1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for PRKAA1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | OriGene Antibodies for PRKAA1 | | OriGene shRNA RFP for PRKAA1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PRKAA1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PRKAA1 | | | OriGene Protein Over-expression Lysate for PRKAA1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for PRKAA1 | | OriGene 3'-UTR Clone for PRKAA1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PRKAA1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PRKAA1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for PRKAA1 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for PRKAA1 | | OriGene Custom Protein Services for PRKAA1 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat PRKAA1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PRKAA1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PRKAA1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PRKAA1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PRKAA1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PRKAA1 |
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| | Antibodies & Assays for PRKAA1 (AMPKA1) |
| | | |  |  |  |  | | | | |
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 |
 |
 | | | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PRKAA1 |
|  |  |  | | | | ThermoFisher Antibody for PRKAA1 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PRKAA1 |
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