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Aliases & Descriptions for PRIM1
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| EC 2.7.7.- 3 | | MGC12308 2 | | p49 2, 3 |
| | | Descriptions |
|---|
| DNA primase 1 2 | | DNA primase 49 kDa subunit 3 | | DNA primase polypeptide 1 2 | | DNA primase small subunit 2 | | DNA primase subunit 48 2 | | primase p49 subunit 2 | | primase polypeptide 1, 49kDa 2 | | primase, DNA, polypeptide 1 (49kDa) 2 |
|
| | Search outside databases for aliases for PRIM1 genePrevious GC identifers: GC12M057097 GC12M057351 GC12M056842 |
Summaries for PRIM1(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for PRIM1: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq] UniProtKB/Swiss-Prot: PRI1_HUMAN, P49642Function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication |
Genomic Location for PRIM1
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the PRIM1 gene 
Entrez Gene cytogenetic band: 12q13 Ensembl cytogenetic band: 12q13.3 HGNC cytogenetic band: 12q13.3PRIM1 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 12 GeneLoc Exon Structure GeneLoc location for GC12M055411:
(about GC identifiers)
Start:
|
55,409,326 bp from pter |
End:
|
55,432,413 bp from pter |
Size:
|
23,088 bases |
Orientation:
|
minus strand |
RefSeq DNA sequence:- NC_000012.10 NT_029419.11
| Proteins for PRIM1
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: PRI1_HUMAN, P49642 (See
protein sequence)Recommended Name: DNA primase small subunit Size: 420 amino acids; 49902 Da
Subunit: Heterodimer of a small subunit and a large subunit
Miscellaneous: The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (By similarity)Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_000937.1
ENSEMBL proteins: ENSP00000350491
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
2 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for PRIM1: Assays for PRIM1: | Protein
Domains/ Families for PRIM1(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry P49642
ProtoNet protein and cluster: P49642 1 Blocks protein family: IPB002755 DNA primase
UniProtKB/Swiss-Prot: PRI1_HUMAN, P49642Similarity: Belongs to the eukaryotic-type primase small subunit family | Gene Function for PRIM1
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_000946
Applied Biosystems Silencer® siRNAs for PRIM1
Sigma-Aldrich siRNA for PRIM1  Sigma-Aldrich shRNA for PRIM1  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_000946                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_000946                                  untagged cDNA clone in CMV expression vector: NM_000946 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_000946
UniProtKB/Swiss-Prot: PRI1_HUMAN, P49642Function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replicationEnzyme Number (IUBMB): EC 2.7.7.- Genatlas biochemistry entry for PRIM1:DNA primase 1,49kDa,replication protein catalyzing the synthesis of Okazaki fragments,smallest subunit of the pol alpha (POLA)/primare complex,amplified in osteosarcoma with CDK4,OS9 and SAS5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6
): About this table | Pathways & Interactions for PRIM1
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
2 Sigma-Aldrich "Your Favorite Gene" Pathways for PRIM1 (Your Favorite Gene powered by Ingenuity) 
5/65 Interacting proteins for PRIM1 (P496422 ENSP000003504913) via UniProtKB, MINT, and/or STRING (see all 65
)About this table
3 Gene Ontology (GO) biological process terms (links to tree view): About this table
|
Drugs & Compounds for PRIM1(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for PRIM1
|
Transcripts for PRIM1(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_000946
Sigma-Aldrich siRNA for PRIM1  Sigma-Aldrich shRNA for PRIM1  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_000946 REFSEQ mRNAs for PRIM1 gene: NM_000946.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_000946               OriGene GFP tagged cDNA clone in CMV expression vector: NM_000946                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_000946                                  untagged cDNA clone in CMV expression vector: NM_000946  Additional cDNA sequence: AK223391.1 AK314866.1 BC005266.1 CR612911.1 CR620245.1 CR621514.1 X74330.1 13 DOTS entries: DT.95170285 DT.412248 DT.121209135 DT.121209306 DT.100722871 DT.121209280 DT.92427484 DT.95170284 DT.100889898 DT.121209220 DT.95170283 DT.97781404 DT.100711588 24/109 AceView cDNA sequences (see all 109
):CA394585 CR612911 CB135075 BQ221799 BP380262 NM_000946 BC005266 BU165208 BU167590 BM452231 CR621514 CR620245 CA952584 BU689163 BU595778 BG940981 BU597283 AW604178 BU662200 BE881634 AI268345 BU184356 CA419908 AW950273
highest scoring ESTs for PRIM1:X74330 BC005266 BE256307 BE537382 BE564598 BE567470 BE733967 BE793427 BF765897 BG388710 Unigene Cluster for PRIM1: Primase, DNA, polypeptide 1 (49kDa) Hs.534339 [show with all ESTs]Unigene Representative Sequence: NM_000946
GeneLoc Exon Structure
5 Alternative Splicing Database (ASD) splice patterns (SP) for PRIM1
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6a | · | 6b | · | 6c | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 | |
| SP1: | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | - |   | - |   | |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP5: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for PRIM1
1 Ensembl transcript including schematic representation: ENST00000338193
|
Expression for PRIM1
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| PRIM1 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for PRIM1
1 / 2 / 3 3 probe-sets matching PRIM1 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: ATGAACTTCA
SOURCE GeneReport for Unigene cluster: Hs.534339
Expression variation in blood from EXPOLDB for PRIM1 |
Orthologs for PRIM1
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for PRIM1 gene from 5/25 species (see all 25
)
| Organism |
Gene |
Locus |
Description |
Human Similarity |
NCBI accessions |
dog (Canis familiaris) |
PRIM11 |
-- |
primase, DNA, polypeptide 1 (49kDa) |
92.56(n) 92.77(a) |
607309 XM_844058.1 XP_849151.1 |
chimpanzee (Pan troglodytes) |
PRIM11 |
-- |
primase, DNA, polypeptide 1 (49kDa) |
99.84(n) 100(a) |
746924 XM_001168535.1 XP_001168535.1 |
cow (Bos taurus) |
PRIM11 |
-- |
primase, DNA, polypeptide 1 (49kDa) |
91.2(n) 90.8(a) |
506240 XM_582661.3 XP_582661.3 |
mouse (Mus musculus) |
Prim11, 5 |
10 (77.00 cM)5
|
DNA primase, p49 subunit1, 5 |
87.61(n)1 91.13(a)1 |
190751 NM_008921.21 NP_032947.11 AI3249825 AK0114565 (see all 13) |
chicken (Gallus gallus) |
PRIM11 |
-- |
primase, polypeptide 1, 49kDa |
63.77(n) 73.91(a) |
426646 XM_424273.2 XP_424273.2 |
About this table Species with no ortholog for PRIM1
ENSEMBL Gene Tree for PRIM1 | Paralogs for PRIM1(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Pseudogenes
- 2 related pseudogenes
|
SNPs/Variants for PRIM1(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for PRIM1 (up to first 250kb)
|
Disorders & Mutations for PRIM1
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 176635
1 Novoseek disease relationship for PRIM1 gene
| Disease |
Score |
Articles |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| cancer |
0.00 |
1 |
10441007 (1) |
About this table
|
Medical News for PRIM1(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for PRIM1 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/22 PubMed articles for PRIM1 gene (see all 22
):- Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12. (PubMed id 8530050)1, 2, 3 Shiratori A....Miyazawa H. (1995)
- Mapping of the human DNA primase 1 (PRIM1) to chromosome 12q13. (PubMed id 9268648)1, 3, 4 Cloutier S.... Yotov W.V. (1997)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- DNA replication in vitro by recombinant DNA-polymerase-alpha- primase. (PubMed id 8026492)3, 4 Stadlbauer F.... Nasheuer H.-P. (1994)
- Amplifications of DNA primase 1 (PRIM1) in human osteosarcoma. (PubMed id 10441007)1, 3 Yotov W.V....Levy E. (1999)
- Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication. (PubMed id 12393188)3 Matheos D....Zannis-Hadjopoulos M. (2002)
- Physical and functional interactions of the tumor suppressor protein p53 and DNA polymerase alpha-primase. (PubMed id 11917009)3 Melle C. and Nasheuer H.P. (2002)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)3 Strausberg R.L....Marra M.A. (2002)
- RPA governs endonuclease switching during processing of Okazaki fragments in eukaryotes. (PubMed id 11473323)3 Bae S.H....Seo Y.S. (2001)
- Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit. (PubMed id 10504261)3 Arezi B....Kuchta R.D. (1999)
|
Search for PRIM1
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing PRIM1
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing PRIM1
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PRIM1(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for PRIM1(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for PRIM1:

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Incremental update: 13 Oct 2009
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