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PPFIA2 Gene

protein-coding   GIFtS: 51

GC12M080155
protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ41378 2
MGC132572 2
Descriptions
PTPRF interacting protein alpha 2 2
PTPRF-interacting protein alpha-2 3
Protein tyrosine phosphatase receptor type f
polypeptide-interacting protein alpha-2 3
liprin-alpha 2 2
protein tyrosine phosphatase, receptor type, f polypeptide
(PTPRF), interacting protein (liprin), alpha 2 2
External Ids
HGNC: 92461
Entrez Gene: 84992
UniProtKB: O753343
Ensembl: ENSG000001392207
Search outside databases for aliases for PPFIA2 gene

Previous GC identifers: GC12M084491 GC12M082017 GC12M081584

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for PPFIA2:
The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting
protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein
tyrosine phosphatases, which are known to be important for axon guidance and mammary gland
development. It has been proposed that liprins are multivalent proteins that form complex
structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine
phosphatases. This protein is most closely related to PPFIA1, a liprin family member known to
interact with the protein phosphatase LAR. The expression of this gene is found to be
downregulated by androgens in a prostate cancer cell line. [provided by RefSeq]

UniProtKB/Swiss-Prot: LIPA2_HUMAN, O75334
Function: Alters PTPRF cellular localization and induces PTPRF clustering. May regulate the
disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at
specific sites on the plasma membrane, possibly regulating their interaction with the
extracellular environment and their association with substrates

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the PPFIA2 gene  

Entrez Gene cytogenetic band: 12q21.31   Ensembl cytogenetic band:  12q21.31   HGNC cytogenetic band: 12q21.31

PPFIA2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M080155:     (about GC identifiers)

Start:
80,177,487 bp from pter
End:
80,677,240 bp from pter
Size:
499,754 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000012.10  NT_019546.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: LIPA2_HUMAN, O75334 (See protein sequence)
Recommended Name: Liprin-alpha-2  
Size: 1257 amino acids; 143142 Da
Subunit: Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the
second PTPase domain of PTPRD, PTPRF and PTPRS
Subcellular location: Cytoplasm. Cell surface. Note=Colocalizes with PTPRF at the cell surface

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003616.2  

    ENSEMBL proteins: 
    ENSP00000327416 ENSP00000385093 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Liprin alpha 2)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm TAS9624153
    GO:0009986 cell surface IEA--
    About this table

    Antibodies for PPFIA2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for PPFIA2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Liprin alpha 2), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibody for PPFIA2

    Assays for PPFIA2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR001660 SAM
     IPR011510 SAM_2


       GeneDecks  PPFIA2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O75334

    ProtoNet protein and cluster: O75334

    2 Blocks protein families:
    IPB001660 Sterile alpha motif SAM
    IPB011510 Sterile alpha motif homology 2


    UniProtKB/Swiss-Prot: LIPA2_HUMAN, O75334
    Domain: The N-terminal coiled coil regions mediate homodimerization preferentially and
    heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate
    heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS
    Similarity: Belongs to the liprin family. Liprin-alpha subfamily
    Similarity: Contains 3 SAM (sterile alpha motif) domains

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PPFIA2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003625

                  Applied Biosystems Silencer® siRNAs for PPFIA2

                  Sigma-Aldrich siRNA and siRNA Panels for PPFIA2  
                         Sigma-Aldrich shRNA Panels and shRNA for PPFIA2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003625
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003625
                                     untagged cDNA clone in CMV expression vector: NM_003625 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003625

    UniProtKB/Swiss-Prot: LIPA2_HUMAN, O75334
    Function: Alters PTPRF cellular localization and induces PTPRF clustering. May regulate the
    disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at
    specific sites on the plasma membrane, possibly regulating their interaction with the
    extracellular environment and their association with substrates

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI12923177
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/7 Interacting proteins for PPFIA2 (ENSP000003274163) via UniProtKB, MINT, and/or STRING (see all 7 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PARK2ENSP000003558653STRING (score=.899)
    SUMO1ENSP000002642813STRING (score=.899)
    UBE2E2ENSP000003383403STRING (score=.899)
    UBE2L3ENSP000003442593STRING (score=.899)
    PPP2CAENSP000002315043STRING (score=.845)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007160 cell-matrix adhesion TAS9624153
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for PPFIA2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PPFIA2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003625

                  Sigma-Aldrich siRNA and siRNA Panels for PPFIA2  
                         Sigma-Aldrich shRNA Panels and shRNA for PPFIA2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003625  

    REFSEQ mRNAs for PPFIA2 gene: 

    NM_003625.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003625  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003625
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003625
                                     untagged cDNA clone in CMV expression vector: NM_003625 

    Additional cDNA sequence: 

    AB210009.1 AF034799.1 AK123372.1 AK126971.1 AK294505.1 AK296380.1 AK297372.1 AK299853.1 
    AK302658.1 AK307524.1 AL157453.1 BC104912.1 BC143485.1 

    11 DOTS entries:

    DT.97792131  DT.409054  DT.100008354  DT.107168  DT.101981713  DT.121182319  DT.121182285  DT.121182277 
    DT.40210520  DT.97800233  DT.97807395 

    24/33 AceView cDNA sequences (see all 33 ):

    BQ027545 AK126971 BM689808 NM_003625 BM661844 AK123372 AL157453 BU153957 
    BU568524 CB051855 BX951956 BQ008801 N45544 AL712783 AF034799 BF002886 
    BX507846 AW014561 BM545514 BQ340238 BM718222 N51002 AL713132 BF525425 

    highest scoring ESTs for PPFIA2:

    AF034799 AK126971 N48694 AA021459 AA885753 AB210009 AI002917 AL712783 AW014561 BF002886 

    Unigene Cluster for PPFIA2:

    Protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
    Hs.506216  [show with all ESTs]
    Unigene Representative Sequence: AB210009


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000333447  ENST00000407050  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    PPFIA2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for PPFIA2

    1 / 2 / 3

    7 probe-sets matching PPFIA2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    33495_at2, 3 U95-A 1 1.00 1.00 0.97 0.65 AF034799 1.00 1.00 1.00 1
    58734_at2, 3 U95-C 1 1.00 1.00 0.97 1.39 N48694 0.80 1.00 0.91 1

    206973_at2, 3 U133-A 1 1.00 1.00 -- -- NM_003625 0.60 1.00 0.82 1

    232073_at2, 3 U133-B 1 0.55 1.00 -- -- AL157453 0.40 1.00 0.76 1

    1558295_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    206973_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    232073_at2 U133Plus2 1 0.55 1.00 -- -- -- -- -- -- --
    GeneDecks  PPFIA2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.506216

    Expression variation in blood from EXPOLDB for PPFIA2

    UniProtKB/Swiss-Prot: LIPA2_HUMAN, O75334
    Tissue specificity: Expressed only in brain

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for PPFIA2 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    PPFIA21   -- protein tyrosine phosphatase, receptor type, f polypeptide more 93.2(n)
    98.43(a)
    475415  XM_532638.2  XP_532638.2 
    chimpanzee
    (Pan troglodytes)
    PPFIA21   -- protein tyrosine phosphatase, receptor type, f polypeptide more 99.79(n)
    100(a)
    452101  XM_509243.2  XP_509243.2 
    rat
    (Rattus norvegicus)
    Ppfia21   -- protein tyrosine phosphatase, receptor type, f polypeptide more 90.29(n)
    98.97(a)
    362876  XM_343204.3  XP_343205.3 
    mouse
    (Mus musculus)
    Ppfia21, 5 105
    protein tyrosine phosphatase, receptor type, f polypeptide more1, 5 90.53(n)1
    98.81(a)1
    3278141  NM_177373.31  NP_796347.21 
     AC1110145  AI8542255  (see all 11)
    chicken
    (Gallus gallus)
    PPFIA21   -- protein tyrosine phosphatase, receptor type, f polypeptide more 84.03(n)
    95.66(a)
    417877  XM_416120.2  XP_416120.2 
    About this table        Species with no ortholog for PPFIA2

    ENSEMBL Gene Tree for PPFIA2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for PPFIA2 gene
    PPFIA42  PPFIA32  PPFIA12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/2104 NCBI SNPs in PPFIA2 are shown (see all 2104 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 726)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs22448021,2
    A,F,H80677880(+) TCCCCG/CTTATA 1 -- ng314Minor allele frequency- C:0.36EU EA WA 416
    rs111148081,2
    F,H80177444(+) AATCAC/GATTTT 1 -- ng514Minor allele frequency- G:0.16EU EA WA 418
    rs116083471,2
    H80177184(+) CATGAA/CCCata 1 -- ng514Minor allele frequency- C:0.00EU EA WA 420
    rs48423901,2
    H80302153(+) ACAAAC/TGCTGC 1 R/H mis14Minor allele frequency- T:0.00EU EA WA 406
    rs24530731,2
    A80679174(-) ACAGTC/GTTTCT 1 -- ng310--------
    rs108623011,2
    A,C,F,H80293712(+) TGCCGC/TAGGAT 1 L/L syn1 ese39Minor allele frequency- N:0.00EA NA EU WA 704
    rs341873671,2
    F80265593(+) GCCAAA/GTTCAG 1 N/N syn11Minor allele frequency- G:0.05NA 76
    rs10009331,2
    A,C,F,H,O80342954(-) TGAATT/CACATA 1 -- int17Minor allele frequency- C:0.41EU EA WA NA 556
    rs24714891,2
    A,C,F,H,O80674120(-) TGTCAC/TGATGC 1 -- int14Minor allele frequency- T:0.36NA WA 256
    rs19224161,2
    A,C,F,H,O80522623(+) ACCCAT/ATTTAA 1 -- int112Minor allele frequency- A:0.30EU EA WA NA 982
    About this table

    HapMap Linkage Disequilibrium images for PPFIA2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603143

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for PPFIA2 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 8499 HGNC: 9246 AceView: PPFIA2 Ensembl:ENSG00000139220 euGenes: HUgn8499
    ECgene: PPFIA2 H-InvDB: PPFIA2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for PPFIA2 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for PPFIA2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for PPFIA2 Antibodies for PPFIA2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for PPFIA2 Browse small molecules at Sigma-Aldrich
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     Recombinant Proteins
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     Search OriGene for PPFIA2
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     Antibodies for PPFIA2

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