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POU2AF1 Gene

protein-coding   GIFtS: 57

GC11M110728
POU class 2 associating factor 1
(Previous names: POU domain class 2, associating factor 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BOB-1 3
BOB1 2
OBF-1 2
OBF1 1, 2, 3, 5
OCA-B 3
OCAB 2
Descriptions
B-cell-specific coactivator OBF-1 3
OCT-binding factor 1 3
POU class 2 associating factor 1 2
POU domain class 2, associating factor 1 1
POU domain, class 2, associating factor 1 2
External Ids
HGNC: 92111
Entrez Gene: 54502
UniProtKB: Q166333
Ensembl: ENSG000001107777
Search outside databases for aliases for POU2AF1 gene

Previous GC identifers: GC11M113575 GC11M112735 GC11M111256 GC11M110760

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633
Function: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It
boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no
intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential
for the response of B-cells to antigens and required for the formation of germinal centers

Gene Wiki entry for POU2AF1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the POU2AF1 gene  

Entrez Gene cytogenetic band: 11q23.1   Ensembl cytogenetic band:  11q23.1   HGNC cytogenetic band: 11q23.1

POU2AF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M110728:     (about GC identifiers)

Start:
110,728,190 bp from pter
End:
110,755,627 bp from pter
Size:
27,438 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_033899.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633 (See protein sequence)
Recommended Name: POU domain class 2-associating factor 1  
Size: 256 amino acids; 27436 Da
Subcellular location: Nucleus
PDB structures from and Proteopedia :
1CQT (3D)    
Secondary accessions: Q14983

Post-translational modifications:

  • Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation
    (Probable)1


  • REFSEQ proteins: NP_006226.2  

    ENSEMBL proteins: 
    ENSP00000228217 ENSP00000376786 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (BOB1)
    Human Recombinant Proteins from Abnova (POU2AF1)
                  OriGene Purified Recombinant Human Protein: POU2AF1 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for POU2AF1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibody Arrays and Antibodies for POU2AF1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (BOB1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (POU2AF1)
    Novus Biologicals Antibodies for POU2AF1

    Assays for POU2AF1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR015389 PD-C2-AF1


       GeneDecks  POU2AF1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q16633

    ProtoNet protein and cluster: Q16633

    UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633
    Similarity: Belongs to the POU2AF1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (POU2AF1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (POU2AF1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_006235

                  Applied Biosystems Silencer® siRNAs for POU2AF1

                  Sigma-Aldrich siRNA for POU2AF1  
                         Sigma-Aldrich shRNA for POU2AF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006235
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006235
                                     untagged cDNA clones in CMV expression vector (see all 2): BC032549 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006235

    UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633
    Function: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It
    boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no
    intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential
    for the response of B-cells to antigens and required for the formation of germinal centers

    Genatlas biochemistry entry for POU2AF1:
    POU domain class 2,associating factor 1,B-cell specific coactivator,specifically associating with
    OCT1 (OTF1) and OCT2,fused with LAZ3/BCL6 in a B-cell leukemia cell line (Karpas 231)

    5 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Pou2af1):

    growth/sizehematopoietic systemimmune systemlife span-post-weaning/agingrenal/urinary system

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003713 transcription coactivator activity TAS7859290
    GO:0005515 protein binding IPI16713566
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  POU2AF1 


    5/21 Interacting proteins for POU2AF1 (ENSP000002282173 Q166331) via UniProtKB, MINT, and/or STRING (see all 21 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    Syk Syk (xeno)P480251EBI-943588, EBI-300116
    POU2F1ENSP000003568363STRING (score=.999)
    POU2F2ENSP000002920773STRING (score=.991)
    SIAH1ENSP000003530903STRING (score=.942)
    IKZF3ENSP000002930683STRING (score=.902)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0006366 transcription from RNA polymerase II promoter TAS7859290
    GO:0006959 humoral immune response TAS7859290
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for POU2AF1
    1 Novoseek chemical compound relationship for POU2AF1 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 0.00 2 10604987 (2)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (POU2AF1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (POU2AF1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_006235

                  Sigma-Aldrich siRNA for POU2AF1  
                         Sigma-Aldrich shRNA for POU2AF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006235  

    REFSEQ mRNAs for POU2AF1 gene: 

    NM_006235.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006235  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006235
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006235
                                     untagged cDNA clones in CMV expression vector (see all 2): BC032549 

    Additional cDNA sequence: 

    AK313573.1 BC022056.1 BC032549.1 CR599556.1 X83504.1 Z47550.1 Z49194.1 

    8 DOTS entries:

    DT.97858926  DT.208246  DT.423232  DT.101977522  DT.302944  DT.99962992  DT.120710296  DT.92427082 

    24/64 AceView cDNA sequences (see all 64 ):

    AI888552 BX462387 AA362508 X83504 BF510638 AA928017 BQ423501 AL560547 
    BX443224 BM795830 CR599556 Z47550 AI707700 BX445716 BI908827 CF121528 
    AW207472 BX342926 BX479626 NM_006235 BG756199 BM927670 AW663547 BM464708 

    highest scoring ESTs for POU2AF1:

    AW499772 AW501101 BF797462 BG341016 BI908827 BI912349 X83504 Z47550 Z49194 AA362508 

    Unigene Cluster for POU2AF1:

    POU class 2 associating factor 1
    Hs.654525  [show with all ESTs]
    Unigene Representative Sequence: Z49194


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000228217  ENST00000393067  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    POU2AF1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for POU2AF1

    1 / 2 / 3

    3 probe-sets matching POU2AF1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    36239_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 Z49194 0.80 1.00 0.91 1

    205267_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006235 0.60 1.00 0.82 1

    205267_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  POU2AF1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GACTTTTCTG

    SOURCE GeneReport for Unigene cluster: Hs.654525

    Expression variation in blood from EXPOLDB for POU2AF1

    UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633
    Tissue specificity: B-cell specific

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for POU2AF1 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    POU2AF11   -- POU class 2 associating factor 1 89.45(n)
    92.97(a)
    489413  XM_546531.2  XP_546531.2 
    chimpanzee
    (Pan troglodytes)
    POU2AF11   -- POU class 2 associating factor 1 98.56(n)
    97.65(a)
    451537  XM_508747.2  XP_508747.1 
    cow
    (Bos taurus)
    POU2AF11   -- POU class 2 associating factor 1 86.98(n)
    91.41(a)
    528475  NM_001075915.1  NP_001069383.1 
    rat
    (Rattus norvegicus)
    Pou2af11   -- POU class 2 associating factor 1 85.29(n)
    87.89(a)
    690528  XM_001074701.1  XP_001074701.1 
    mouse
    (Mus musculus)
    Pou2af11, 5 95
    POU domain, class 2, associating factor 11, 5 86.07(n)1
    88.67(a)1
    189851  NM_011136.21  NP_035266.11 
     AF2412575  AK0885875  (see all 9)
    About this table        Species with no ortholog for POU2AF1

    ENSEMBL Gene Tree for POU2AF1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/207 NCBI SNPs in POU2AF1 are shown (see all 207 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 112)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10427521,2
    A,C,F,H,O110728321(-) GTCACT/CATTAA 1 -- ut31 ese313Minor allele frequency- C:0.31MN NA EU EA WA NS 1146
    rs65892231,2
    A,C,F,H110729399(+) CTTTTC/TTCCCT 1 -- ut31 trp37Minor allele frequency- T:0.02EU EA WA NA 560
    rs43562681,2
    A,C,F,H110755860(+) ACGGAA/GAAAGA 1 -- ng31 trp37Minor allele frequency- G:0.50EU EA WA NA 542
    rs49385101,2
    A,C,F,H110756686(+) TTTATA/CAATGA 1 -- ng31 tfbs34Minor allele frequency- C:0.48EU EA WA 420
    --
    rs112138661,2
    A,C,F110756459(+) TGTTCG/CTCTTC 1 -- ng313Minor allele frequency- C:0.02NA 140
    rs105021431,2
    C,F,H110727923(+) AAATGG/ATACGC 1 -- ng5117Minor allele frequency- A:0.18NS EU EA WA NA 1112
    --
    rs124175561,2
    A,C,F110728655(+) cacaaA/Ggtcag 1 -- ut31 ese31Minor allele frequency- G:0.38NS 40
    --
    rs112138471,2
    A,C,F110728615(+) cctgtG/Aatccc 1 -- ut31 ese31Minor allele frequency- A:0.17NS 40
    --
    rs118258751,2
    A,C,F110728726(+) tagccA/Gggcat 1 -- ut31 ese31Minor allele frequency- G:0.17NS 40
    rs45659311,2
    C,F,H110755725(+) TCTTTT/CAATAT 1 -- ng317Minor allele frequency- C:0.01EU EA WA NA 548
    About this table

    HapMap Linkage Disequilibrium images for POU2AF1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601206

    UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633
  • A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell
    leukemia. Translocation t(3;11)(q27;q23) with BCL6
  • 2 Novoseek disease relationships for POU2AF1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    hodgkin disease 37.39 3 17562619 (1), 11280769 (1), 15796964 (1)
    lymphoma 23.12 3 16186795 (1), 19224997 (1)
    About this table

    1 PharmGKB disease relationship for POU2AF1 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872 12730115
    About this table

    Human Genome Epidemiology Navigator: POU2AF1 (2 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/71 PubMed articles for POU2AF1 gene (see all 71 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 5450 HGNC: 9211 AceView: POU2AF1 Ensembl:ENSG00000110777 euGenes: HUgn5450
    ECgene: POU2AF1 H-InvDB: POU2AF1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for POU2AF1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for POU2AF1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for POU2AF1 Antibodies for POU2AF1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for POU2AF1 Browse small molecules at Sigma-Aldrich
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
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