POU2AF1 Gene
protein-coding GIFtS: 52
GCID: GC11M111222
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POU class 2 associating factor 1(Previous names: POU domain class 2, associating factor 1 )
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Aliases
for POU2AF1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| POU Class 2 Associating Factor 11 2 | | POU Domain Class 2, Associating Factor 11 | | OBF11 2 3 5 | | BOB12 | | BOB-12 3 | | OBF-12 | | OCA-B2 3 | | OCAB2 | | B-Cell-Specific Coactivator OBF-12 3 | | POU Domain Class 2-Associating Factor 12 | | OCT-Binding Factor 12 3 | | POU Domain, Class 2, Associating Factor 12 |
Export aliases for POU2AF1 gene to outside databasesPrevious GC identifers: GC11M113575 GC11M112735 GC11M111256 GC11M110760 GC11M110728 GC11M107147 |
Summaries
for POU2AF1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633Function: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. Itrecognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required forthe formation of germinal centers
Gene Wiki entry for POU2AF1
|
Genomic Views
for POU2AF1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000011.9 NC_018922.1 NT_033899.8
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the POU2AF1 gene promoter:
TBP AML1a MyoD HNF-3beta POU3F2 C/EBPalpha FOXL1 c-Ets-1 MEF-2A CHOP-10
Other transcription factors
Search SABiosciences Chromatin IP Primers for POU2AF1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat POU2AF1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 11q23.1 Ensembl cytogenetic band: 11q23.1 HGNC cytogenetic band: 11q23.1POU2AF1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 11 GeneLoc Exon Structure GeneLoc location for GC11M111222: view genomic region
(about GC identifiers)
Start:
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111,222,977 bp from pter |
End:
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111,326,355 bp from pter |
Size:
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103,379 bases |
Orientation:
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minus strand |
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Proteins
for POU2AF1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633 (See
protein sequence)Recommended Name: POU domain class 2-associating factor 1 Size: 256 amino acids; 27436 Da
Subcellular location: Nucleus
1 PDB 3D structure from  and Proteopedia  for POU2AF1:1CQT (3D)
Secondary accessions: B2R8Z9 Q14983Explore the universe of human proteins at neXtProt for POU2AF1: NX_Q16633
Post-translational modifications:
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (Probable)1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q16633
POU2AF1 Protein expression data from MOPED and PaxDb: --
REFSEQ proteins: NP_006226.2
ENSEMBL proteins: ENSP00000376786 ENSP00000433527
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005634 | nucleus |
IEA | -- |
POU2AF1 for ontologies About GeneDecksing
POU2AF1 Antibody Products:
Assay Products for POU2AF1: |
Protein
Domains /
Families
for POU2AF1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
POU2AF1 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q16633ProtoNet protein and cluster: Q16633 UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633Similarity: Belongs to the POU2AF1 family |
Function
for POU2AF1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633Function: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. Itrecognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required forthe formation of germinal centers Genatlas biochemistry entry for POU2AF1:POU domain class 2,associating factor 1,B-cell specific coactivator,specifically associating with OCT1 (OTF1) andOCT2,fused with LAZ3/BCL6 in a B-cell leukemia cell line (Karpas 231)
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for POU2AF1 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for POU2AF1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POU2AF1 |
Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table
POU2AF1 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for POU2AF1:
Animal Models:
Mouse knock-outs for POU2AF1: Pou2af1tm1Pjln Pou2af1tm1Pmt Pou2af1tm1Rgr
6 MGI mutant phenotypes (inferred from 3 alleles ) (MGI details for Pou2af1):
POU2AF1 for phenotypes About GeneDecksing
|
Pathways & Interactions
for POU2AF1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for POU2AF1
5/15 Interacting proteins for POU2AF1 (Q166333) via UniProtKB, MINT, STRING, and/or I2D (see all 15)About this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table
POU2AF1 for ontologies About GeneDecksing
|
Drugs & Compounds
for POU2AF1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
POU2AF1 for compounds About GeneDecksing
 Browse Tocris compounds for POU2AF1
1 Novoseek chemical compound relationship for POU2AF1 gene About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| tyrosine |
0 |
2 |
10604987 (2) |
Search CenterWatch for drugs/clinical trials and news about POU2AF1 / OBF1
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Transcripts
for POU2AF1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for POU2AF1 gene: NM_006235.2 Unigene Cluster for POU2AF1: POU class 2 associating factor 1 Hs.654525 [show with all ESTs]Unigene Representative Sequence: Z4919412 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000393067(uc001plg.4) ENST00000525584 ENST00000531398 ENST00000530793
ENST00000525662 ENST00000526535 ENST00000525499 ENST00000515029(uc001plh.1)
ENST00000530229 ENST00000525527 ENST00000529065 ENST00000525890
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for POU2AF1 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for POU2AF1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: POU2AF1 (NM_006235) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for POU2AF1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat POU2AF1 |
Additional cDNA sequence: AK313573.1 BC032549.1 X83504.1 Z47550.1 Z49194.1 4 DOTS entries: DT.97858926 DT.208246 DT.99962992 DT.92427082 24/64 AceView cDNA sequences (see all 64): Z47550 BQ423501 AL560547 AI888552 X83504 BX443224 BF510638 BX462387 AA928017 AA362508 BM795830 CR599556 BM452578 AW407993 AI707700 BX445716 BM927670 BM464708 AI890020 BG756199 AW405850 BM464990 BI908827 CF121528
GeneLoc Exon Structure
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Expression
for POU2AF1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| POU2AF1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GACTTTTCTG
About this image
Genevestigator expression for POU2AF1
SOURCE GeneReport for Unigene cluster: Hs.654525
UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633Tissue specificity: B-cell specific
SABiosciences Expression via Pathway-Focused PCR Arrays including POU2AF1:
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for POU2AF1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat POU2AF1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat POU2AF1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat POU2AF1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POU2AF1 |
Orthologs
for POU2AF1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for POU2AF1 gene from 3/9 species (see all 9) About this table
ENSEMBL Gene Tree for POU2AF1 (if available)
TreeFam Gene Tree for POU2AF1 (if available) |
Paralogs
for POU2AF1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for POU2AF1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 11 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for POU2AF1 (111222977 - 111326355 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for POU2AF1: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing POU2AF1 |
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Disorders
/ Diseases
for POU2AF1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
POU2AF1 for disorders About GeneDecksing
OMIM gene information: 601206
OMIM disorders: --
UniProtKB/Swiss-Prot: OBF1_HUMAN, Q16633
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia.Translocation t(3;11)(q27;q23) with BCL6
20/23  diseases for POU2AF1 (see all 23): About MalaCardscommon variable immunodeficiency primary effusion lymphoma b-cell lymphomas systemic lupus erythematosus
lupus erythematosus chronic lymphocytic leukemia acute myeloid leukemia lymphocytic leukemia
myeloid leukemia kaposi's sarcoma agammaglobulinemia hodgkin's lymphoma
multiple myeloma mediastinitis multiple sclerosis seminoma
leukemia myeloma sarcoma t-cell leukemia
1 disease from the University of Copenhagen DISEASES database for POU2AF1:Lymphoma 2 Novoseek disease relationships for POU2AF1 gene About this table
Human Genome Epidemiology (HuGE) Navigator: POU2AF1 (7 documents)
Export disorders for POU2AF1 gene to outside databases
|
Publications
for POU2AF1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for POU2AF1 gene, integrated from 9 sources (see all 82):
(articles sorted by number of sources associating them with POU2AF1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B. (PubMed id 7623806)1, 2, 9 Luo Y. and Roeder R.G. (1995)
- Assignment of the human gene for Oct-binding factor-1 (OBF1), a B-cell-specific coactivator of octamer-binding transcription factors 1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization. (PubMed id 8617501)1, 3, 9 Junker S....Tommerup N. (1996)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1. (PubMed id 11483517)1, 2 Tiedt R.... Matthias P. (2001)
- Regulation of BOB.1/OBF.1 stability by SIAH. (PubMed id 11483518)1, 2 Boehm J.... Wirth T. (2001)
- A B-cell coactivator of octamer-binding transcription factors. (PubMed id 7779176)1, 2 Gstaiger M.... Hovens C.M. (1995)
- Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation. (PubMed id 8574789)1, 2 Galiegue-Zouitina S.... Kerckaert J.-P. (1995)
- OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer- binding proteins. (PubMed id 7859290)1, 2 Strubin M.... Matthias P. (1995)
- Bob1 (OCA-B/OBF-1) differential transactivation of the B cell-specific B29 (Ig beta) and mb-1 (Ig alpha) promoters. (PubMed id 11907094)1, 9 Malone C.S. and Wall R. (2002)
- Coactivator OBF-1 makes selective contacts with both the POU-specific domain and the POU homeodomain and acts as a molecular clamp on DNA. (PubMed id 9819426)1, 9 Sauter P. and Matthias P. (1998)
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External Searches for POU2AF1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing POU2AF1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing POU2AF1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing POU2AF1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for POU2AF1 gene:
Search GeneIP for patents involving POU2AF1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for POU2AF1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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variant library, vector shuttling | | | OriGene Custom Antibody Services for POU2AF1 | | OriGene Custom Protein Services for POU2AF1 | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat POU2AF1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing POU2AF1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat POU2AF1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat POU2AF1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat POU2AF1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat POU2AF1 |
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| | | | Search Tocris compounds for POU2AF1 |
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 | | | POU2AF1 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POU2AF1 |
|  |  |  | | | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat POU2AF1 |
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