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Aliases for POLR2B Gene

Aliases for POLR2B Gene

  • Polymerase (RNA) II Subunit B 2 3
  • Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa 3 5
  • DNA-Directed RNA Polymerase II 140 KDa Polypeptide 3 4
  • DNA-Directed RNA Polymerase II Subunit B 3 4
  • RNA Polymerase II Subunit B2 3 4
  • RNA Polymerase II Subunit 2 3 4
  • EC 2.7.7.6 4 63
  • Polymerase (RNA) II (DNA Directed) Polypeptide B (140kD) 2
  • RNA Polymerase II Second Largest Subunit 3
  • EC 2.7.7 63
  • HRPB140 3
  • POL2RB 3
  • RPB2 3

External Ids for POLR2B Gene

Previous GeneCards Identifiers for POLR2B Gene

  • GC04P057648
  • GC04P057841
  • GC04P057760
  • GC04P057685
  • GC04P057538
  • GC04P053798
  • GC04P057844

Summaries for POLR2B Gene

Entrez Gene Summary for POLR2B Gene

  • This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

GeneCards Summary for POLR2B Gene

POLR2B (Polymerase (RNA) II Subunit B) is a Protein Coding gene. Diseases associated with POLR2B include eumycotic mycetoma and fusariosis. Among its related pathways are Epstein-Barr virus infection and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.. GO annotations related to this gene include poly(A) RNA binding and DNA-directed RNA polymerase activity. An important paralog of this gene is POLR3B.

UniProtKB/Swiss-Prot for POLR2B Gene

  • DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Gene Wiki entry for POLR2B Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for POLR2B Gene

Genomics for POLR2B Gene

Regulatory Elements for POLR2B Gene

Enhancers for POLR2B Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around POLR2B on UCSC Golden Path with GeneCards custom track

Promoters for POLR2B Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around POLR2B on UCSC Golden Path with GeneCards custom track

Genomic Location for POLR2B Gene

Chromosome:
4
Start:
56,977,722 bp from pter
End:
57,031,168 bp from pter
Size:
53,447 bases
Orientation:
Plus strand

Genomic View for POLR2B Gene

Genes around POLR2B on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
POLR2B Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for POLR2B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for POLR2B Gene

Proteins for POLR2B Gene

  • Protein details for POLR2B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P30876-RPB2_HUMAN
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB2
    Protein Accession:
    P30876
    Secondary Accessions:
    • A8K1A8
    • Q8IZ61

    Protein attributes for POLR2B Gene

    Size:
    1174 amino acids
    Molecular mass:
    133897 Da
    Quaternary structure:
    • Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with WDR82. Interacts with MEN1.
    Miscellaneous:
    • The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity).

neXtProt entry for POLR2B Gene

Proteomics data for POLR2B Gene at MOPED

Post-translational modifications for POLR2B Gene

  • Ubiquitination at Lys 95, Lys 130, Lys 134, Lys 146, Lys 151, Lys 199, Lys 214, Lys 264, Lys 340, Lys 421, Lys 445, Lys 457, Lys 578, Lys 730, Lys 756, Lys 821, Lys 831, Lys 847, Lys 869, Lys 917, Lys 934, Lys 942, Lys 988, Lys 993, Lys 1010, Lys 1036, Lys 1143, and Lys 1156
  • Modification sites at PhosphoSitePlus

Other Protein References for POLR2B Gene

Antibody Products

Domains & Families for POLR2B Gene

Gene Families for POLR2B Gene

Suggested Antigen Peptide Sequences for POLR2B Gene

Graphical View of Domain Structure for InterPro Entry

P30876

UniProtKB/Swiss-Prot:

RPB2_HUMAN :
  • Belongs to the RNA polymerase beta chain family.
Family:
  • Belongs to the RNA polymerase beta chain family.
genes like me logo Genes that share domains with POLR2B: view

Function for POLR2B Gene

Molecular function for POLR2B Gene

GENATLAS Biochemistry:
polymerase II,RNA (DNA directed) large polypeptide B (140kDa)
UniProtKB/Swiss-Prot CatalyticActivity:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
UniProtKB/Swiss-Prot Function:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Enzyme Numbers (IUBMB) for POLR2B Gene

Gene Ontology (GO) - Molecular Function for POLR2B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003682 chromatin binding IEA --
GO:0003899 DNA-directed RNA polymerase activity IEA --
genes like me logo Genes that share ontologies with POLR2B: view
genes like me logo Genes that share phenotypes with POLR2B: view

Animal Model Products

CRISPR Products

miRNA for POLR2B Gene

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for POLR2B Gene

Localization for POLR2B Gene

Subcellular locations from UniProtKB/Swiss-Prot for POLR2B Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for POLR2B Gene COMPARTMENTS Subcellular localization image for POLR2B gene
Compartment Confidence
nucleus 5
chloroplast 2
cytoskeleton 2
cytosol 2
mitochondrion 1

Gene Ontology (GO) - Cellular Components for POLR2B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005665 DNA-directed RNA polymerase II, core complex IDA 9852112
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with POLR2B: view

Pathways & Interactions for POLR2B Gene

SuperPathways for POLR2B Gene

Superpath Contained pathways
1 Formation of the HIV-1 Early Elongation Complex
2 RNA Polymerase II Transcription Initiation And Promoter Clearance
3 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
4 Assembly of RNA Polymerase-II Initiation Complex
5 Signaling by FGFR2 in disease
genes like me logo Genes that share pathways with POLR2B: view

Gene Ontology (GO) - Biological Process for POLR2B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000398 mRNA splicing, via spliceosome TAS --
GO:0006281 DNA repair TAS --
GO:0006283 transcription-coupled nucleotide-excision repair TAS --
GO:0006370 7-methylguanosine mRNA capping TAS --
GO:0008380 RNA splicing TAS --
genes like me logo Genes that share ontologies with POLR2B: view

No data available for SIGNOR curated interactions for POLR2B Gene

Drugs & Compounds for POLR2B Gene

(8) Drugs for POLR2B Gene - From: ApexBio and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
Cytidine triphosphate Experimental Pharma 0
Diphosphate Experimental Pharma 0
Guanosine triphosphate Experimental Pharma 0
Uridine triphosphate Experimental Pharma 0

(1) Additional Compounds for POLR2B Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Thymidine 5'-triphosphate
  • 2'-Deoxythymidine 5'-triphosphate
  • 2'-Deoxythymidine triphosphate
  • 5'-TTP
  • 5-Methyl-dUTP
  • Deoxy-TTP
365-08-2

(2) ApexBio Compounds for POLR2B Gene

Compound Action Cas Number
PSI-6206 Inhibitor of HCV RNA polymerase,potent and selective 863329-66-2
PSI-7977 Antiviral agents for chronic HCV infection 1190307-88-0
genes like me logo Genes that share compounds with POLR2B: view

Transcripts for POLR2B Gene

Unigene Clusters for POLR2B Gene

Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for POLR2B Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b · 11c ^ 12a ·
SP1: -
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13: - - -
SP14: - - -
SP15:

ExUns: 12b ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b ^ 19 ^ 20 ^ 21a · 21b ^ 22a · 22b ^ 23a · 23b ^ 24a · 24b ^ 25 ^ 26
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:

Relevant External Links for POLR2B Gene

GeneLoc Exon Structure for
POLR2B
ECgene alternative splicing isoforms for
POLR2B

Expression for POLR2B Gene

mRNA expression in normal human tissues for POLR2B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for POLR2B Gene

This gene is overexpressed in Lymph node (17.1) and Peripheral blood mononuclear cells (9.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for POLR2B Gene



SOURCE GeneReport for Unigene cluster for POLR2B Gene Hs.602757

genes like me logo Genes that share expression patterns with POLR2B: view

Protein tissue co-expression partners for POLR2B Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for POLR2B Gene

Orthologs for POLR2B Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for POLR2B Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia POLR2B 35
  • 94.32 (n)
  • 99.91 (a)
POLR2B 36
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia POLR2B 35
  • 94.92 (n)
  • 99.91 (a)
POLR2B 36
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Polr2b 35
  • 90.35 (n)
  • 99.91 (a)
Polr2b 16
Polr2b 36
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia POLR2B 35
  • 99.66 (n)
  • 100 (a)
POLR2B 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Polr2b 35
  • 89.89 (n)
  • 100 (a)
oppossum
(Monodelphis domestica)
Mammalia POLR2B 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 100 (a)
OneToMany
-- 36
  • 81 (a)
OneToMany
chicken
(Gallus gallus)
Aves POLR2B 35
  • 83.82 (n)
  • 99.4 (a)
POLR2B 36
  • 99 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia POLR2B 36
  • 99 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia polr2b 35
  • 82.61 (n)
  • 99.06 (a)
Str.15006 35
African clawed frog
(Xenopus laevis)
Amphibia MGC68569 35
zebrafish
(Danio rerio)
Actinopterygii Dr.16327 35
polr2b 35
  • 77.98 (n)
  • 97.27 (a)
polr2b 36
  • 96 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13862 35
fruit fly
(Drosophila melanogaster)
Insecta RpI135 37
  • 27 (a)
RpII140 37
  • 86 (a)
RpIII128 37
  • 36 (a)
RpII140 35
  • 72.35 (n)
  • 86.18 (a)
RpII140 36
  • 85 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003648 35
  • 69.31 (n)
  • 86.35 (a)
worm
(Caenorhabditis elegans)
Secernentea C26E6.4 37
  • 78 (a)
F09F7.3 37
  • 36 (a)
F14B4.3 37
  • 29 (a)
rpb-2 35
  • 70.2 (n)
  • 79.13 (a)
rpb-2 36
  • 77 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AFR404C 35
  • 60.06 (n)
  • 60.86 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B05577g 35
  • 61.35 (n)
  • 61.18 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RPB2 35
  • 61.81 (n)
  • 61.29 (a)
RPB2 36
  • 56 (a)
OneToMany
RPB2 38
thale cress
(Arabidopsis thaliana)
eudicotyledons NRPB2 35
  • 64.57 (n)
  • 68.04 (a)
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.3309 35
barley
(Hordeum vulgare)
Liliopsida Hv.4411 35
rice
(Oryza sativa)
Liliopsida Os.25542 35
Os03g0646800 35
  • 64.35 (n)
  • 69.28 (a)
wheat
(Triticum aestivum)
Liliopsida Ta.12287 35
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.11524 35
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rpb2 35
  • 62.59 (n)
  • 63.69 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.1138 36
  • 82 (a)
OneToOne
bread mold
(Neurospora crassa)
Ascomycetes NCU05120 35
  • 59.4 (n)
  • 62.59 (a)
Species with no ortholog for POLR2B:
  • Actinobacteria (Mycobacterium tuberculosis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for POLR2B Gene

ENSEMBL:
Gene Tree for POLR2B (if available)
TreeFam:
Gene Tree for POLR2B (if available)

Paralogs for POLR2B Gene

Paralogs for POLR2B Gene

(3) SIMAP similar genes for POLR2B Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with POLR2B: view

Variants for POLR2B Gene

Sequence variations from dbSNP and Humsavar for POLR2B Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type
rs1277278 -- 56,998,760(+) AATTG(G/T)CATTT intron-variant
rs1277281 -- 56,989,926(-) AAAAG(C/T)AGACA intron-variant
rs1277282 -- 56,989,653(-) GAGAC(C/T)TTCTT intron-variant
rs1277283 -- 56,989,475(-) ACCAA(A/T)ATTAG intron-variant
rs1277284 -- 56,989,195(-) ACTTT(A/C)CCCAA intron-variant

Variation tolerance for POLR2B Gene

Residual Variation Intolerance Score: 3.05% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.89; 18.44% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for POLR2B Gene

HapMap Linkage Disequilibrium report
POLR2B

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for POLR2B Gene

Disorders for POLR2B Gene

MalaCards: The human disease database

(3) MalaCards diseases for POLR2B Gene - From: DISEASES

Disorder Aliases PubMed IDs
eumycotic mycetoma
  • eumycetoma
fusariosis
  • fusarium infection
macular degeneration, age-related, 1
  • macular degeneration, age-related, 2
- elite association - COSMIC cancer census association via MalaCards

Relevant External Links for POLR2B

Genetic Association Database (GAD)
POLR2B
Human Genome Epidemiology (HuGE) Navigator
POLR2B
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
POLR2B
genes like me logo Genes that share disorders with POLR2B: view

No data available for UniProtKB/Swiss-Prot and Genatlas for POLR2B Gene

Publications for POLR2B Gene

  1. Primary structure of the second largest subunit of human RNA polymerase II (or B). (PMID: 1518060) Acker J. … Kedinger C. (J. Mol. Biol. 1992) 2 3 4 67
  2. Identification of proteins interacting with the RNAPII FCP1 phosphatase: FCP1 forms a complex with arginine methyltransferase PRMT5 and it is a substrate for PRMT5-mediated methylation. (PMID: 15670829) Amente S. … Majello B. (FEBS Lett. 2005) 3 23
  3. Chromosomal localization of human RNA polymerase II subunit genes. (PMID: 8034326) Acker J. … Kedinger C. (Genomics 1994) 2 3
  4. Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. (PMID: 25281560) Lambert J.P. … Gingras A.C. (J Proteomics 2015) 3
  5. Meta-analysis of genome-wide association studies of adult height in East Asians identifies 17 novel loci. (PMID: 25429064) He M. … Qi L. (Hum. Mol. Genet. 2015) 3

Products for POLR2B Gene

Sources for POLR2B Gene

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