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POLR2B Gene

protein-coding   GIFtS: 68
GCID: GC04P057844

Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa

(Previous name: polymerase (RNA) II (DNA directed) polypeptide B (140kD))
  See POLR2B-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa1 2     Polymerase (RNA) II (DNA Directed) Polypeptide B (140kD)1
DNA-Directed RNA Polymerase II 140 KDa Polypeptide2 3     RPB22
DNA-Directed RNA Polymerase II Subunit B2 3     DNA Directed RNA Polymerase II 140 KDa Polypeptide, RNA Polymerase II
Subunit 2, RNA Polymerase II Second Largest Subunit2
RNA Polymerase II Subunit 22 3     DNA-Directed RNA Polymerase II Subunit RPB22
RNA Polymerase II Subunit B22 3     hRPB1402
EC 2.7.7.63 8     hsRPB22
POL2RB2 5     EC 2.7.78

External Ids:    HGNC: 91881   Entrez Gene: 54312   Ensembl: ENSG000000473157   OMIM: 1806615   UniProtKB: P308763   

Export aliases for POLR2B gene to outside databases

Previous GC identifers: GC04P057648 GC04P057841 GC04P057760 GC04P057685 GC04P057538 GC04P053798


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for POLR2B Gene:
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing
messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a
structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template
and the newly synthesized RNA. (provided by RefSeq, Jul 2008)

GeneCards Summary for POLR2B Gene:
POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140kDa) is a protein-coding gene. Diseases associated with POLR2B include eumycotic mycetoma, and mycetoma. GO annotations related to this gene include chromatin binding and ribonucleoside binding. An important paralog of this gene is POLR3B.

UniProtKB/Swiss-Prot: RPB2_HUMAN, P30876
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside
triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and
many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the
polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA
polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2
is part of the core element with the central large cleft, the clamp element that moves to open and close the
cleft and the jaws that are thought to grab the incoming DNA template (By similarity)

Gene Wiki entry for POLR2B Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000004.11  NC_018915.2  NT_022853.16  
Regulatory elements:
   Regulatory transcription factor binding sites in the POLR2B gene promoter:
         TBP   FOXF2   HTF   CUTL1   NF-kappaB   Tal-1beta   E47   C/EBPalpha   NF-kappaB1   ITF-2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidPOLR2B promoter sequence
   Search Chromatin IP Primers for POLR2B

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat POLR2B


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q12   Ensembl cytogenetic band:  4q12   HGNC cytogenetic band: 4q12

POLR2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
POLR2B gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P057844:  view genomic region     (about GC identifiers)

Start:
57,843,888 bp from pter      End:
57,897,334 bp from pter
Size:
53,447 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: RPB2_HUMAN, P30876 (See protein sequence)
Recommended Name: DNA-directed RNA polymerase II subunit RPB2  
Size: 1174 amino acids; 133897 Da
Subunit: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with WDR82.
Interacts with MEN1
Miscellaneous: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably
involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium
ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity)
Secondary accessions: A8K1A8 Q8IZ61

Explore the universe of human proteins at neXtProt for POLR2B: NX_P30876

Explore proteomics data for POLR2B at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys95, Lys130, Lys134, Lys146, Lys151, Lys199, Lys214, Lys264, Lys340, Lys421,
                                 Lys445, Lys457, Lys578, Lys730, Lys756, Lys821, Lys831, Lys847, Lys869, Lys917 (see all 28)
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for POLR2B (P30876) (see all 41)
     FGEMERD  PQIGDKF  IFIGPTY  THCEIHP 


    See POLR2B Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_000929.1  
    ENSEMBL proteins: 
     ENSP00000408638   ENSP00000370625   ENSP00000391096   ENSP00000391452   ENSP00000407728  
     ENSP00000312735  
    Reactome Protein details: P30876

    POLR2B Human Recombinant Protein Products:

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    POLR: RNA polymerase subunits

    Selected InterPro protein domains (see all 10):
     IPR007120 DNA-dir_RNA_pol_su2_6
     IPR007641 RNA_pol_Rpb2_7
     IPR007644 RNA_pol_bsu_protrusion
     IPR015712 DNA-dir_RNA_pol_su2
     IPR007645 RNA_pol_Rpb2_3

    Graphical View of Domain Structure for InterPro Entry P30876

    ProtoNet protein and cluster: P30876

    4 Blocks protein domains:
    IPB007120 RNA polymerase Rpb2
    IPB007121 RNA polymerase
    IPB007646 RNA polymerase Rpb2
    IPB007647 RNA polymerase Rpb2


    UniProtKB/Swiss-Prot: RPB2_HUMAN, P30876
    Similarity: Belongs to the RNA polymerase beta chain family


    Find genes that share domains with POLR2B           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: RPB2_HUMAN, P30876
    Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside
    triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and
    many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the
    polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA
    polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2
    is part of the core element with the central large cleft, the clamp element that moves to open and close the
    cleft and the jaws that are thought to grab the incoming DNA template (By similarity)
    Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

         Genatlas biochemistry entry for POLR2B:
    polymerase II,RNA (DNA directed) large polypeptide B (140kDa)

         Enzyme Numbers (IUBMB): EC 2.7.7.61 2 EC 2.7.72

         Gene Ontology (GO): Selected molecular function terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001055contributes to RNA polymerase II activity IBA--
    GO:0003677DNA binding IEA--
    GO:0003682chromatin binding IEA--
    GO:0003899DNA-directed RNA polymerase activity ----
    GO:0005515protein binding IPI17998332
         
    Find genes that share ontologies with POLR2B           About GenesLikeMe


    Phenotypes:
         5 GenomeRNAi human phenotypes for POLR2B:
     Decreased G3BP1 protein expres  Decreased TP53 protein express  Decreased viability of wild-ty  Increased cell death in HCC-19 
     Increased circadian period len 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for POLR2B
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       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for POLR2B

    miRNA
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    miRTarBase miRNAs that target POLR2B:
    hsa-mir-615-3p (MIRT040387), hsa-mir-455-3p (MIRT037880), hsa-mir-30a-5p (MIRT028617), hsa-mir-124-3p (MIRT023207)

    Block miRNA regulation of human, mouse, rat POLR2B using miScript Target Protectors
    2 qRT-PCR Assays for microRNAs that regulate POLR2B:
    hsa-miR-1283 hsa-miR-4282
    SwitchGear 3'UTR luciferase reporter plasmidPOLR2B 3' UTR sequence
    Inhib. RNA
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    Gene Editing
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    Clone
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    GenScript: all cDNA clones in your preferred vector: POLR2B (NM_000938)
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    Flow Cytometry
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    eBioscience FlowRNA Probe Sets ( VA1-12325) for POLR2B 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    RPB2_HUMAN, P30876: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2
    cytoskeleton1

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005665DNA-directed RNA polymerase II, core complex IDA9852112
    GO:0005730NOT nucleolus IDA--

    Find genes that share ontologies with POLR2B           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for POLR2B About   (see all 26)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Formation of RNA Pol II elongation complex
    mRNA Capping0.93
    Pausing and recovery of Tat-mediated HIV elongation0.72
    RNA Pol II CTD phosphorylation and interaction with CE0.93
    Tat-mediated HIV elongation arrest and recovery0.72
    RNA Pol II CTD phosphorylation and interaction with CE0.93
    Transcription of the HIV genome0.71
    Formation of the Early Elongation Complex0.77
    Abortive elongation of HIV-1 transcript in the absence of Tat0.70
    2Purine metabolism (KEGG)
    Purine metabolism0.38
    ATP ITP metabolism0.31
    Pyrimidine metabolism0.38
    3DNA Repair
    Transcription-coupled NER (TC-NER)0.90
    Formation of transcription-coupled NER (TC-NER) repair complex0.65
    Nucleotide Excision Repair0.90
    DNA Repair0.45
    Dual incision reaction in TC-NER0.65
    4mRNA Splicing - Major Pathway
    mRNA Splicing - Major Pathway0.78
    mRNA Splicing0.78
    Processing of Capped Intron-Containing Pre-mRNA0.78
    mRNA Splicing - Minor Pathway0.38
    5Assembly of RNA Polymerase-II Initiation Complex
    Assembly of RNA Polymerase-II Initiation Complex0.91
    Protein Acetylation and Deacetylation0.51
    Transcription of mRNA0.91
    Crosstalk Between CARM1 and ESRs0.39


    Find genes that share SuperPaths with POLR2B           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for POLR2B (see all 17)
        Nuclear Receptor Activation by Vitamin-A
    Telomerase Components in Cell Signaling
    Androgen Signaling
    NF-kappaB Activation by Viruses
    Crosstalk Between CARM1 and ESRs

    3 GeneGo (Thomson Reuters) Pathways for POLR2B
        DNA damage Role of Brca1 and Brca2 in DNA repair
    ATP/ITP metabolism
    Transcription Ligand-Dependent Transcription of Retinoid-Target genes

    1 BioSystems Pathway for POLR2B
        Eukaryotic Transcription Initiation

    Selected Reactome Pathways for POLR2B (see all 31)
        Pausing and recovery of Tat-mediated HIV elongation
    Tat-mediated HIV elongation arrest and recovery
    Formation of RNA Pol II elongation complex
    Tat-mediated elongation of the HIV-1 transcript
    RNA Polymerase II Transcription Initiation And Promoter Clearance


    Selected Kegg Pathways  (Kegg details for POLR2B) (see all 6):
        Purine metabolism
    Pyrimidine metabolism
    Metabolic pathways
    RNA polymerase
    Huntington's disease

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for POLR2B
    Interactions:

        GeneGlobe Interaction Network for POLR2B

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for POLR2B (P308762, 3 ENSP000003127354) via UniProtKB, MINT, STRING, and/or I2D (see all 517)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CTDP1Q9Y5B02, 3, ENSP000002995434MINT-51646 MINT-51650 I2D: score=4 STRING: ENSP00000299543
    CALM1P621583I2D: score=1 
    CALM2P621583I2D: score=1 
    CALM3P621583I2D: score=1 
    WWOXQ9NZC72, 3, ENSP000003861614MINT-72766 I2D: score=3 STRING: ENSP00000386161
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 13):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000398mRNA splicing, via spliceosome TAS--
    GO:0006281DNA repair TAS--
    GO:0006283transcription-coupled nucleotide-excision repair TAS--
    GO:0006289nucleotide-excision repair TAS--
    GO:0006351transcription, DNA-templated ----

    Find genes that share ontologies with POLR2B           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for POLR2B (RPB2)

    8 HMDB Compounds for POLR2B    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    Cytidine triphosphatecytidine 5'-(tetrahydrogen triphosphate) (see all 15)65-47-4--
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--
    MagnesiumMagnesium (see all 2)7439-95-4--
    Phosphoric acidacide phosphorique (FRENCH) (see all 20)7664-38-2--
    Pyrophosphate(4-)Diphosphoric acid ion (see all 10)14000-31-8--
    Thymidine 5'-triphosphate2'-Deoxythymidine 5'-triphosphate (see all 13)365-08-2--
    Uridine triphosphate5'-UTP (see all 7)63-39-8--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for POLR2B gene: 
    NM_000938.1  

    Unigene Cluster for POLR2B:

    Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
    Hs.602757  [show with all ESTs]
    Unigene Representative Sequence: AK295132
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000450656 ENST00000381227 ENST00000495311 ENST00000431623 ENST00000441246(uc011cae.1)
    ENST00000433463 ENST00000497845 ENST00000478188(uc003hcm.1) ENST00000510355
    ENST00000464918 ENST00000484821 ENST00000314595(uc003hcl.1 uc011caf.1)

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    hsa-miR-1283 hsa-miR-4282
    SwitchGear 3'UTR luciferase reporter plasmidPOLR2B 3' UTR sequence
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    Primer
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      QuantiTect SYBR Green Assays in human, mouse, rat POLR2B
      QuantiFast Probe-based Assays in human, mouse, rat POLR2B
    Flow Cytometry
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    eBioscience FlowRNA Probe Sets ( VA1-12325) for POLR2B 

    Additional mRNA sequence: 

    AF055028.1 AK098271.1 AK289823.1 AK294901.1 AK295132.1 AK299090.1 BC023503.2 BC046499.1 
    X63563.1 

    Selected DOTS entries (see all 25):

    DT.100674865  DT.97760173  DT.65285131  DT.100706253  DT.100674870  DT.91806226  DT.121219395  DT.95170547 
    DT.95278266  DT.121219269  DT.121219296  DT.121219317  DT.100639411  DT.101978157  DT.121219386  DT.91916126 
    DT.92461397  DT.92461407  DT.95278263  DT.95313534  DT.97760174  DT.100674866  DT.121219282  DT.121219304 

    Selected AceView cDNA sequences (see all 414):

    BM728262 AL705459 BM995142 BE467961 BM453107 BU619780 BM699556 AF055028 
    BQ688324 BM972682 BM769012 BU631118 BQ215678 BU520866 AW262915 BX490282 
    AL043215 BQ020611 BC023503 BM818298 BU615587 CR591583 CA429140 AI819119 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for POLR2B (see all 15)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b · 11c ^ 12a ·
    SP1:                          -                                                                                                                                 
    SP2:                                                                                                                                                            
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 12b ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b ^ 19 ^ 20 ^ 21a · 21b ^ 22a · 22b ^ 23a · 23b ^ 24a · 24b ^ 25 ^ 26
    SP1:                                                                                                                                    
    SP2:                                                                                                                                    
    SP3:                                                                                                                                    
    SP4:                                                                                                                                    
    SP5:                                                                                                                                    


    ECgene alternative splicing isoforms for POLR2B

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    POLR2B expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTTTTCTTCT
    POLR2B Expression
    About this image


    POLR2B expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 8) fully expand
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Cerebral Cortex
     
     Thymus (Hematopoietic System)
             Thymus
     
     Gut Tube (Gastrointestinal Tract)
             Foregut
     
     Ovary (Reproductive System)
             Primary Oocyte Primary Follicle
     
     Kidney (Urinary System)
             Metanephros
    POLR2B Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    POLR2B Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.602757
        Custom PCR Arrays for POLR2B
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POLR2B

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for POLR2B gene from Selected species (see all 28)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Polr2b1 , 5 polymerase (RNA) II (DNA directed) polypeptide B1, 5 90.35(n)1
    99.91(a)1
      5 (41.66 cM)5
    2313291  NM_153798.21  NP_722493.21 
     773101475 
    chicken
    (Gallus gallus)
    Aves POLR2B1 polymerase (RNA) II (DNA directed) polypeptide B, 14 more 83.82(n)
    99.4(a)
      422621  NM_001006448.1  NP_001006448.1 
    lizard
    (Anolis carolinensis)
    Reptilia POLR2B6
    polymerase (RNA) II (DNA directed) polypeptide B, ...
    99(a)
    1 ↔ 1
    GL343257.1(1844453-1870885)
    African clawed frog
    (Xenopus laevis)
    Amphibia MGC685692 hypothetical protein MGC68569 82.65(n)    BC060458.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.163272 Transcribed sequence with strong similarity to protein more 79.11(n)    BI427984.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta RpII1401 , 3 transcription from Pol II promoter
    DNA-directed more3
    RNA polymerase II 140kD subunit1
    86(a)
    (best of 3)3
    72.35(n)1
    86.18(a)1
      88A93
    417211  NM_057358.31  NP_476706.11 
    worm
    (Caenorhabditis elegans)
    Secernentea C26E6.43
    rpb-21
    DNA-directed RNA polymerase II3
    rpb-21
    78(a)
    (best of 3)3
    70.2(n)1
    79.13(a)1
      III(5011393-5015852)3
    1756681  NM_065646.41  NP_498047.11 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes RPB2(YOR151C)4
    RPB21
    RNA polymerase II second largest subunit B150, part more4
    RPB21
    61.81(n)1
    61.29(a)1
      15(616671-612997)4
    8543221, 4  NP_014794.31  NP_014794.14 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons NRPB21 NRPB2 64.57(n)
    68.04(a)
      828259  NM_118291.3  NP_193902.1 
    rice
    (Oryza sativa)
    Liliopsida Os.255422 Oryza sativa mRNA for RNA polymerase II 72.81(n)    AK111443.1 


    ENSEMBL Gene Tree for POLR2B (if available)
    TreeFam Gene Tree for POLR2B (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for POLR2B gene
    POLR3B2  POLR1B2  
    3 SIMAP similar genes for POLR2B using alignment to 5 protein entries:     RPB2_HUMAN (see all proteins):
    DKFZp686D10173    POLR3B    POLR1B

    Find genes that share paralogs with POLR2B           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for POLR2B (see all 999)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 4 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs48651671,2
    C,H--57748282(+) GCCCCG/ACAGCC 1 -- us2k113Minor allele frequency- A:0.01NA WA CSA EA 378
    rs1837685931,2
    --57750958(+) GGAGGC/TGGAGT 1 -- int10--------
    rs1884313111,2
    --57751058(+) AGTCTA/TGTGTA 1 -- int10--------
    rs1503990381,2
    --57751118(+) TTTTCA/TTATAG 1 -- int10--------
    rs1809038911,2
    --57751161(+) TCTCTC/GTATTC 1 -- int10--------
    rs1847254491,2
    --57751510(+) TTTGGC/TTGGGT 1 -- int10--------
    rs170819371,2
    C,F,H--57751515(+) CTGGGT/CAATAT 1 -- int18Minor allele frequency- C:0.13NA NS EA 680
    rs1145169401,2
    F--57751587(+) GCTCAC/TGCCTG 1 -- int12Minor allele frequency- T:0.03WA NA 238
    rs1907164931,2
    --57751648(+) AGTTCC/GAGAGC 1 -- int10--------
    rs117323941,2
    C,F,A,H--57751887(+) TCCACA/GGTGGG 1 -- int118Minor allele frequency- N:0.00NS EA NA CSA WA 1297

    HapMap Linkage Disequilibrium report for POLR2B (57843888 - 57897334 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for POLR2B: --
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing POLR2B
    DNA2.0 Custom Variant and Variant Library Synthesis for POLR2B

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 180661    OMIM disorders: --

    2 diseases for POLR2B:    
    About MalaCards
    eumycotic mycetoma    mycetoma

    1 disease from the University of Copenhagen DISEASES database for POLR2B:
    Eumycotic mycetoma

    Find genes that share disorders with POLR2B           About GenesLikeMe

    Genetic Association Database (GAD): POLR2B
    Human Genome Epidemiology (HuGE) Navigator: POLR2B (4 documents)

    Export disorders for POLR2B gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for POLR2B gene, integrated from 10 sources (see all 197):
    (articles sorted by number of sources associating them with POLR2B)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Primary structure of the second largest subunit of human RNA polymerase II (or B). (PubMed id 1518060)1, 2, 3 Acker J.... Kedinger C. (J. Mol. Biol. 1992)
    2. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    3. Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk. (PubMed id 19237606)1, 4 Michiels S....Benhamou S. (Carcinogenesis 2009)
    4. Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. (PubMed id 17998332)1, 2 Lee J.H. and Skalnik D.G. (Mol. Cell. Biol. 2008)
    5. Polymorphism discovery in 62 DNA repair genes and haplotype associations with risks for lung and head and neck cancers. (PubMed id 17494052)1, 4 Michiels S....Benhamou S. (Carcinogenesis 2007)
    6. Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. (PubMed id 14992727)1, 2 Hughes C.M.... Meyerson M. (Mol. Cell 2004)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    8. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. (PubMed id 9852112)1, 2 Kershnar E.... Chiang C.-M. (J. Biol. Chem. 1998)
    9. Large-scale concatenation cDNA sequencing. (PubMed id 9110174)1, 2 Yu W.... Gibbs R.A. (Genome Res. 1997)
    10. Chromosomal localization of human RNA polymerase II subunit genes. (PubMed id 8034326)1, 3 Acker J....Kedinger C. (Genomics 1994)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 5431 HGNC: 9188 AceView: POLR2B Ensembl:ENSG00000047315 euGenes: HUgn5431
    ECgene: POLR2B Kegg: 5431 H-InvDB: POLR2B

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for POLR2B Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for POLR2B gene:
    Search GeneIP for patents involving POLR2B

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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