POLR2A Gene
protein-coding GIFtS: 67
GCID: GC17P007387
|
|
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa(Previous name: polymerase (RNA) II (DNA directed) polypeptide A (220kD)...)
(Previous symbol: POLR2)
| |
Aliases
for POLR2A gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa1 2 | | Polymerase (RNA) II (DNA Directed) Polypeptide A (220kD)1 | | POLR21 2 3 | | RPBh12 | | POLRA1 2 | | RPO22 | | RPB11 2 | | RpIILS2 | | DNA-Directed RNA Polymerase II Subunit A2 3 | | DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 KdSubunit2 | | DNA-Directed RNA Polymerase III Largest Subunit2 3 | | DNA-Directed RNA Polymerase II Subunit RPB12 | | RNA Polymerase II Subunit B12 3 | | HRPB2201 | | RNA-Directed RNA Polymerase II Subunit RPB12 3 | | HsRPB11 | | EC 2.7.7.63 8 | | EC 2.7.7.483 | | RPOL22 5 | | |
Export aliases for POLR2A gene to outside databasesPrevious GC identifers: GC17M007800 GC17P008117 GC17P007330 GC17P007588 GC17P007329 |
Summaries
for POLR2A gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for POLR2A:
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNAin eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that areessential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated inactively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerasesubunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.(provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: RPB1_HUMAN, P24928Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursorsand many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. PolII is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elementsthat move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp elementthat moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the startof transcription, a single stranded DNA template strand of the promoter is positioned within the central active sitecleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought topromote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site byswitching from straight to bent conformations at each step of nucleotide addition. During transcription elongation,Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status ofthe C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors thatregulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerasewhen associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase forthe viral RNA circular genome
Gene Wiki entry for POLR2A
|
Genomic Views
for POLR2A gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000017.10 NC_018928.1 NT_010718.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the POLR2A gene promoter:
TBP p53 HNF-4alpha2 Ik-3 HNF-4alpha1 RORalpha1 MZF-1 HOXA5 c-Myb
Other transcription factors
Search SABiosciences Chromatin IP Primers for POLR2A
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat POLR2A |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 17p13.1 Ensembl cytogenetic band: 17p13.1 HGNC cytogenetic band: 17p13.1POLR2A Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 17 GeneLoc Exon Structure GeneLoc location for GC17P007387: view genomic region
(about GC identifiers)
Start:
|
7,387,685 bp from pter |
End:
|
7,417,933 bp from pter |
Size:
|
30,249 bases |
Orientation:
|
plus strand |
|
Proteins
for POLR2A gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RPB1_HUMAN, P24928 (See
protein sequence)Recommended Name: DNA-directed RNA polymerase II subunit RPB1 Size: 1970 amino acids; 217176 Da
Subunit: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminaldomain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C (By similarity).Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFbcomplex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (viaC-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and withSETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts withMEN1; the unphosphorylated form, or phosphorylated at 'Ser-2' does not interact. Interacts with DDX5
Subcellular location: Nucleus
Miscellaneous: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probablyinvolves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium iontemporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. Theribonucleoside triphosphate is transferred by a rotation to the nucleotide addition (A) site for pairing with thetemplate DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit whichpermanently coordinate a second magnesium ion
3 PDB 3D structures from  and Proteopedia  for POLR2A:2GHQ (3D)
2GHT (3D)
2LTO (3D)
Secondary accessions: A6NN93 B9EH88Explore the universe of human proteins at neXtProt for POLR2A: NX_P24928
Post-translational modifications:
The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylationactivates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and ismediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcriptioninitiation by triggering dissociation from DNA. Phosphorylation also takes place at 'Ser-7' of the heptapepdtiderepeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. Thephosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases,and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed1
Dephosphorylated by the protein phosphatase CTDSP11
Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). Following UV treatment, the elongating formof RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination isfacilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repairmachinery1
Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation atSer-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylationand transcription initiation. CTD methylation may facilitate the expression of select RNAs1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P24928
4/23 DME Specific Peptides for POLR2A (P24928) (see all 23)
POLR2A Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_000928.1
ENSEMBL proteins: ENSP00000314949 ENSP00000461879 Reactome Protein details: P24928
Human Recombinant Protein Products:
Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table
POLR2A for ontologies About GeneDecksing
POLR2A Antibody Products:
Assay Products for POLR2A: |
Protein
Domains /
Families
for POLR2A gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
POLR2A for domains About GeneDecksing
5/10 InterPro domains/families (see all 10):Graphical View of Domain Structure for InterPro Entry P24928ProtoNet protein and cluster: P24928 4 Blocks protein families: IPB000684 Eukaryotic RNA polymerase II heptapeptide repeat IPB006592 RNA polymerase I subunit A IPB007075 RNA polymerase Rpb1 IPB007083 RNA polymerase Rpb1
UniProtKB/Swiss-Prot: RPB1_HUMAN, P24928Similarity: Belongs to the RNA polymerase beta' chain family |
Function
for POLR2A gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RPB1_HUMAN, P24928Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursorsand many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. PolII is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elementsthat move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp elementthat moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the startof transcription, a single stranded DNA template strand of the promoter is positioned within the central active sitecleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought topromote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site byswitching from straight to bent conformations at each step of nucleotide addition. During transcription elongation,Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status ofthe C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors thatregulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerasewhen associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase forthe viral RNA circular genomeCatalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) Genatlas biochemistry entry for POLR2A:polymerase II,RNA (DNA directed) large polypeptide A (220kDa)
Enzyme Numbers (IUBMB): EC 2.7.7.61 2 EC 2.7.7.481
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for POLR2A (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for POLR2A
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: POLR2A (NM_000937) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for POLR2A | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat POLR2A |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POLR2A |
Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7): About this table
POLR2A for ontologies About GeneDecksing
3 GenomeRNAi human phenotypes for POLR2A:
Animal Models:
Mouse knock-outs for POLR2A: Polr2atm1Cdn Polr2atm2Cdn
13 MGI mutant phenotypes (inferred from 5 alleles ) (MGI details for Polr2a):
POLR2A for phenotypes About GeneDecksing
|
Pathways & Interactions
for POLR2A gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/28 super-pathways (see all 28) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Formation of RNA Pol II elongation complex | | | 2 | mRNA Splicing - Major Pathway | | | 3 | Assembly of RNA Polymerase-II Initiation Complex | | | 4 | HIV Infection | | | 5 | Nucleotide Excision Repair | |
Pathway sources
See GeneCards unified pathways
Show all pathways
3 EMD Millipore Pathways for POLR2A
5/17 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for POLR2A (see all 17)
3 
Cell Signaling Technology (CST) Pathways for POLR2A
3  GeneGo (Thomson Reuters) Pathways for POLR2A
2  BioSystems Pathways for POLR2A
5/49 
Reactome Pathways for POLR2A (see all 49)
5 
Kegg Pathways (Kegg details for POLR2A):
POLR2A for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for POLR2A
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/980 Interacting proteins for POLR2A (P249281, 2, 3 ENSP000003149494) via UniProtKB, MINT, STRING, and/or I2D (see all 980)| Interactant | Interaction Details |
|---|
| GeneCard | External ID(s) |
|---|
| DDX5 | P178441, 2, 3, ENSP000002257924 | EBI-295301,EBI-351962 MINT-7945693 I2D:
score=1 STRING: ENSP00000225792 | | SMN2 | Q166372, 3, ENSP000003701194 | MINT-7945693 I2D:
score=2 STRING: ENSP00000370119 | | CPSF1 | Q105702, 3, ENSP000003393534 | MINT-7945693 I2D:
score=5 STRING: ENSP00000339353 | | CTDP1 | Q9Y5B02, 3, ENSP000002995434 | MINT-19371 I2D:
score=4 STRING: ENSP00000299543 | | ACTB | P607092, 3, ENSP000003499604 | MINT-7945693 I2D:
score=3 STRING: ENSP00000349960 |
About this table
Gene Ontology (GO): 5/14 biological process terms (GO ID links to tree view) (see all 14): About this table
POLR2A for ontologies About GeneDecksing
|
Drugs & Compounds
for POLR2A gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for POLR2A
8 HMDB Compounds for POLR2A About this table
Search CenterWatch for drugs/clinical trials and news about POLR2A / RPB1
|
Transcripts
for POLR2A gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for POLR2A gene: NM_000937.4 Unigene Cluster for POLR2A: Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Hs.270017 [show with all ESTs]Unigene Representative Sequence: NM_0009379 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000322644(uc002ghf.4) ENST00000572844(uc002ghe.3) ENST00000576952
ENST00000575547 ENST00000576114 ENST00000576718 ENST00000574158 ENST00000573603
ENST00000576553
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for POLR2A (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for POLR2A
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: POLR2A (NM_000937) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for POLR2A | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat POLR2A |
Additional cDNA sequence: AK293777.1 AK304882.1 BC067295.1 BC107140.2 BC137231.1 M21610.1 X63564.1 24/29 DOTS entries (see all 29): DT.447371 DT.97800541 DT.99977706 DT.102831278 DT.86858634 DT.115898 DT.95243495 DT.99963102 DT.95351102 DT.100773913 DT.95243528 DT.95258876 DT.100702565 DT.120961743 DT.120961754 DT.120961778 DT.120961781 DT.120961799 DT.75167926 DT.86842845 DT.91745121 DT.95113267 DT.97788563 DT.97858347 24/354 AceView cDNA sequences (see all 354): BQ056284 BP366290 BM550332 AA368202 CD244180 BQ378467 R73715 BM704836 BM768667 BQ064935 BM470885 BQ946063 H42773 BU501761 AI867684 BM714182 BM423630 BU678159 BM716018 CA433266 BM553445 BF591985 BM010523 BQ324662
GeneLoc Exon Structure
|
Expression
for POLR2A gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| POLR2A expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TCTCCCAGCT
About this image
See POLR2A Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for POLR2A
SOURCE GeneReport for Unigene cluster: Hs.270017
SABiosciences Custom PCR Arrays for POLR2A
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for POLR2A
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat POLR2A | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat POLR2A | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat POLR2A | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POLR2A |
Orthologs
for POLR2A gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the last universal common ancestor (LUCA).
Orthologs for POLR2A gene from 9/41 species (see all 41) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
lizard
(Anolis carolinensis) |
Reptilia |
POLR2A6 |
-- |
98(a) |
1 ↔ 1 |
GL343660.1(95184-122172) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.71892 |
Xenopus laevis transcribed sequence with moderate similarity more |
78.66(n) |
|
CA971909.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
polr2a1 |
polymerase (RNA) II (DNA directed) polypeptide A |
78.2(n)
95.36(a) |
|
553347 XM_677590.2 XP_682682.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
RpII2151 , 3 |
transcription from Pol II promoter
DNA-directed more3
RNA polymerase II 215kD subunit1 |
76(a)3
67.45(n)1
74.6(a)1 |
|
321001 NM_078569.21 NP_511124.11 |
worm
(Caenorhabditis elegans) |
Secernentea |
ama-11 , 3 |
RNA polymerase II3
Protein AMA-11 |
69(a)3
63.77(n)1
72.12(a)1 |
|
IV(4248386-4257980)3
1771901 NM_068122.51 NP_500523.31 |
baker's yeast
(Saccharomyces cerevisiae) |
Saccharomycetes |
RPO21(YDL140C)4
RPO211 |
RNA polymerase II largest subunit B220, part of central more4
Rpo21p1 |
53.53(n)1
56.64(a)1 |
|
4(210561-205360)4
8514151, 4 NP_010141.11, 4 |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
NRPB11 |
DNA-directed RNA polymerase II subunit RPB1 |
58.03(n)
62.49(a) |
|
829734 NM_119746.3 NP_195305.2 |
rice
(Oryza sativa) |
Liliopsida |
AK062907.12 |
-- |
78.23(n) |
|
AK062907.1 |
E. coli
(Escherichia coli) |
Gamma proteobacteria |
rpoC6 |
RNA polymerase, beta prime subunit |
17(a) |
1 ↔ 1 |
Chromosome(4183373-4187596) |
ENSEMBL Gene Tree for POLR2A (if available)
TreeFam Gene Tree for POLR2A (if available) |
Paralogs
for POLR2A gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for POLR2A gene
- LOC1005070032 CHAMP12
2 SIMAP similar genes for POLR2A using alignment to 2 protein entries: RPB1_HUMAN (see all proteins):POLR2 POLR3A
POLR2A for paralogs About GeneDecksing
|
Genomic Variants
for POLR2A gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 17 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for POLR2A (7387685 - 7417933 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for POLR2A: --
Human Gene Mutation Database (HGMD): POLR2A
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing POLR2A |
|
Disorders
/ Diseases
for POLR2A gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
POLR2A for disorders About GeneDecksing
OMIM gene information: 180660
OMIM disorders: --
20/23  diseases for POLR2A (see all 23): About MalaCardsla crosse encephalitis myxoid liposarcoma renal clear cell carcinoma chikungunya
liposarcoma cockayne syndrome huntington's disease conjunctivitis
encephalitis hepatocellular carcinoma prostate cancer breast cancer
myeloma pneumonia carcinoma prostatitis
tuberculosis immunodeficiency hepatitis malaria
1 disease from the University of Copenhagen DISEASES database for POLR2A:La Crosse encephalitis Human Genome Epidemiology (HuGE) Navigator: POLR2A (5 documents)
Export disorders for POLR2A gene to outside databases
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Publications
for POLR2A gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
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PubMed articles for POLR2A gene, integrated from 9 sources (see all 381):
(articles sorted by number of sources associating them with POLR2A) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The human gene encoding the largest subunit of RNA polymerase II. (PubMed id 7622068)1, 2, 9 Mita K.... Hayashi A. (1995)
- MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity. (PubMed id 19106100)1, 2 Liu L....Nakao M. (2009)
- Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. (PubMed id 17998332)1, 2 Lee J.H. and Skalnik D.G. (2008)
- Transcription of hepatitis delta virus RNA by RNA polymerase II. (PubMed id 18032511)1, 2 Chang J.... Taylor J. (2008)
- The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis. (PubMed id 16491129)1, 2 Moniaux N....Batra S.K. (2006)
- Identification and characterization of a novel human histone H3 lysine 36 specific methyltransferase. (PubMed id 16118227)1, 2 Sun X.-J.... Chen Z. (2005)
- Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1. (PubMed id 16314571)1, 2 Li M.... Zhou P. (2005)
- Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. (PubMed id 15144186)1, 2 Brill L.M....Peters E.C. (2004)
- Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells. (PubMed id 14684736)1, 2 Yang L.... Cao X. (2004)
- Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. (PubMed id 14992727)1, 2 Hughes C.M....Meyerson M. (2004)
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External Searches for POLR2A gene
(in PubMed,
OMIM, and NCBI Bookshelf)
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Genome Databases showing POLR2A gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
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Other Databases showing POLR2A gene
(According to HUGE)
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Specialized Databases showing POLR2A gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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| Name | Description |
| PharmGKB entry for POLR2A | Pharmacogenomics, SNPs, Pathways |
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| Patent Information for POLR2A gene:
Search GeneIP for patents involving POLR2A
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Products
for POLR2A gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
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