POLN Gene
protein-coding GIFtS: 47
GCID: GC04M002040
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polymerase (DNA directed) nu
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Aliases
for POLN gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Polymerase (DNA Directed) Nu1 2 | | POL4P2 | | DNA Polymerase N2 | | DNA Polymerase Nu2 | | DNA Polymerase POL4P2 | | EC 2.7.7.73 |
Export aliases for POLN gene to outside databasesPrevious GC identifers: GC04U900224 GC04M002035 |
Summaries
for POLN gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
-- |
Genomic Views
for POLN gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000004.11 NC_018915.1 NT_006051.18
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the POLN gene promoter:
Bach1 FOXO3 Nkx5-1 FOXO3b AREB6 POU2F1 POU2F1a FOXO3a TGIF Ik-1
Other transcription factors
Search SABiosciences Chromatin IP Primers for POLN
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat POLN |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 4p16.3 Ensembl cytogenetic band: 4p16.3 HGNC cytogenetic band: 4p16.3POLN Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 4 GeneLoc Exon Structure GeneLoc location for GC04M002040: view genomic region
(about GC identifiers)
Start:
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2,073,645 bp from pter |
End:
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2,243,848 bp from pter |
Size:
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170,204 bases |
Orientation:
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minus strand |
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Proteins
for POLN gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: DPOLN_HUMAN, Q7Z5Q5 (See
protein sequence)Recommended Name: DNA polymerase nu Size: 900 amino acids; 100307 Da
Subcellular location: Nucleus
Sequence caution: Sequence=BAD18421.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact;
Secondary accessions: A2A336 Q4TTW4 Q6ZNF4Explore the universe of human proteins at neXtProt for POLN: NX_Q7Z5Q5
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q7Z5Q5
POLN Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_861524.2
ENSEMBL proteins: ENSP00000435506 ENSP00000426401 ENSP00000372316
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005634 | nucleus |
IEA | -- |
POLN for ontologies About GeneDecksing
POLN Antibody Products:
Assay Products for POLN: |
Protein
Domains /
Families
for POLN gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
POLN for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q7Z5Q5ProtoNet protein and cluster: Q7Z5Q5 1 Blocks protein family: IPB001098 DNA-directed DNA polymerase
UniProtKB/Swiss-Prot: DPOLN_HUMAN, Q7Z5Q5Similarity: Belongs to the DNA polymerase type-A family |
Function
for POLN gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: DPOLN_HUMAN, Q7Z5Q5Catalytic activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)Enzyme Number (IUBMB): EC 2.7.7.71
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for POLN (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for POLN
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: POLN (NM_181808) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for POLN | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat POLN |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POLN |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003677 | DNA binding |
IEA | -- | | GO:0003887 | DNA-directed DNA polymerase activity |
IEA | -- |
POLN for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for POLN:
|
Pathways & Interactions
for POLN gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for POLN
STRING Interaction
Network Preview (showing 5 interactants - click image to see 10)
5/11 Interacting proteins for POLN (Q7Z5Q53 ENSP000003723164) via UniProtKB, MINT, STRING, and/or I2D (see all 11)About this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table
POLN for ontologies About GeneDecksing
|
Drugs & Compounds
for POLN gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for POLN
Search CenterWatch for drugs/clinical trials and news about POLN / DPOLN
|
Transcripts
for POLN gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for POLN gene: NM_181808.2 Unigene Cluster for POLN: Polymerase (DNA directed) nu Hs.368454 [show with all ESTs]Unigene Representative Sequence: AK13123913 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000511885(uc003ger.2) ENST00000503371 ENST00000511098 ENST00000514521
ENST00000511777 ENST00000509623 ENST00000514858(uc010ich.1) ENST00000508568
ENST00000503601 ENST00000515357(uc011bvi.1) ENST00000510344 ENST00000506518
ENST00000382865(uc010icg.1)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for POLN (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for POLN
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: POLN (NM_181808) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for POLN | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat POLN |
Additional cDNA sequence: AF044578.1 AK131239.1 AK303673.1 AL122048.1 AY136549.1 8 DOTS entries: DT.100675141 DT.91714320 DT.91707338 DT.92414447 DT.121236923 DT.437045 DT.65284201 DT.121236963 24/71 AceView cDNA sequences (see all 71): BM843545 AV646359 BC003648 AA442824 BC025356 AF040964 CA421871 AI674093 BQ024208 AL709166 AK056134 AA630380 NM_024511 AW169985 BX114515 AI627212 BX400052 CR749640 AI628516 BG678161 AY136549 AW513701 CA865481 NM_181808
GeneLoc Exon Structure
|
Expression
for POLN gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| POLN expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TCGCACTGAG
About this image
See POLN Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for POLN
SOURCE GeneReport for Unigene cluster: Hs.368454
UniProtKB/Swiss-Prot: DPOLN_HUMAN, Q7Z5Q5Tissue specificity: Highly expressed in testis and heart. Weakly expressed in skeletal muscle
SABiosciences Custom PCR Arrays for POLN
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for POLN
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat POLN | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat POLN | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat POLN | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POLN |
Orthologs
for POLN gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the last universal common ancestor (LUCA).
Orthologs for POLN gene from 6/19 species (see all 19) About this table
ENSEMBL Gene Tree for POLN (if available)
TreeFam Gene Tree for POLN (if available) |
Paralogs
for POLN gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for POLN gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 4 variations for POLN
4 CNVs: 80144 98607 6235 91878 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing POLN |
|
Disorders
/ Diseases
for POLN gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
POLN for disorders About GeneDecksing
OMIM gene information: 610887
OMIM disorders: --
5  diseases for POLN: About MalaCardsfanconi's anemia anemia pneumonia tuberculosis
mycobacterium tuberculosis
Human Genome Epidemiology (HuGE) Navigator: POLN (2 documents)
Export disorders for POLN gene to outside databases
|
Publications
for POLN gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for POLN gene, integrated from 9 sources (see all 25):
(articles sorted by number of sources associating them with POLN) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308. (PubMed id 12794064)1, 2, 3, 9 Marini F.... Wood R.D. (2003)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Evidence for the involvement of human DNA polymerase N in the repair of DNA interstrand cross-links. (PubMed id 19908865)1, 9 Zietlow L....Bessho T. (2009)
- c-Jun N-terminal kinase-mediated Rad18 phosphorylation facilitates Poly recruitment to stalled replication forks. (PubMed id 22456510)1 Barkley L.R....Vaziri C. (2012)
- Enhancement of human DNA polymerase y activity and fid elity is dependent upon a bipartite interaction with the Werner syndrome protein . (PubMed id 23045531)1 Maddukuri L....Eoff R.L. (2012)
- Epstein-Barr virus BPLF1 deubiquitinates PCNA and atte nuates polymerase y recruitment to DNA damage sites. (PubMed id 22623772)1 Whitehurst C.B....Pagano J.S. (2012)
- Genetic variants in DNA repair genes and the risk of c utaneous malignant melanoma in melanoma-prone families with/without CDKN2A mutat ions. (PubMed id 21671477)1 Liang X.S....Yang X.R. (2012)
- Preparation of site-specific T=mCG cis-syn cyclobutane dimer-containing template and its error-free bypass by yeast and human polymera se y. (PubMed id 22262850)1 Song Q....Taylor J.S. (2012)
- ATR-mediated phosphorylation of DNA polymerase I^ is n eeded for efficient recovery from UV damage. (PubMed id 21242293)1 GAPhler T....Lehmann A.R. (2011)
- A comprehensive genetic association study of Alzheimer disease in African Americans. (PubMed id 22159054)1 Logue M.W....Farrer L.A. (2011)
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External Searches for POLN gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
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Genome Databases showing POLN gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing POLN gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing POLN gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for POLN | Pharmacogenomics, SNPs, Pathways | | NIEHS-SNPs | http://egp.gs.washington.edu/data/poln/ |
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About This Section
| Patent Information for POLN gene:
Search GeneIP for patents involving POLN
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for POLN gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
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 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for POLN | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for POLN | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for POLN | | | OriGene Protein Over-expression Lysate for POLN | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for POLN | | OriGene 3'-UTR Clone for POLN | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for POLN | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for POLN | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for POLN | | OriGene Custom Protein Services for POLN | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat POLN | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing POLN | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat POLN | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat POLN | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat POLN | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat POLN |
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| | | | Search Tocris compounds for POLN |
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 | | | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for POLN |
|  |  |  | | | | Search ThermoFisher Antibodies for POLN |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat POLN |
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