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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

PLK1S1 Gene

protein-coding   GIFtS: 46
GCID: GC20P021056

polo-like kinase 1 substrate 1

(Previous names: non-protein coding RNA 153, chromosome 20 open reading...)
(Previous symbols: NCRNA00153, C20orf19)
 Explore 2 diseases affiliated with
PLK1S1 via our new
 Human Malady Compendium 
Biological research products
for PLK1S1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA

Quality score for the ORGUL clustered with this gene is 3

Aliases
Polo-Like Kinase 1 Substrate 11 2 3     HT0131
C20orf191 2 3     Chromosome 20 Open Reading Frame 191
NCRNA001531 2 3     Non-Protein Coding RNA 1531
Kiz1 2     Centrosomal Protein Kizuna2
Kizuna1 2     KIZ3

External Ids:    HGNC: 158651   Entrez Gene: 558572   Ensembl: ENSG000000889707   UniProtKB: Q2M2Z53   
ORGUL members:         
NONCODE:n378391 n379068 n378153 n378004 n378282 see all 9    

Export aliases for PLK1S1 gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for PLK1S1:
The protein encoded by this gene localizes to centrosomes, strengthening and stabilizing the pericentriolar region
prior to spindle formation. The encoded protein usually remains with the mother centrosome after centrosomal
duplication. Sevral transcript variants encoding different isoforms have been found for this gene. (provided by
RefSeq, Feb 2013)

UniProtKB/Swiss-Prot: KIZ_HUMAN, Q2M2Z5
Function: Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the
forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar
material around the centriole




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000020.10  NC_018931.1  NT_011387.8  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for PLK1S1
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for PLK1S1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PLK1S1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20p11.23   Ensembl cytogenetic band:  20p11.23   HGNC cytogenetic band: 20p11.23

PLK1S1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PLK1S1 gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20P021056:  view genomic region     (about GC identifiers)

Start:
21,106,624 bp from pter      End:
21,227,260 bp from pter
Size:
120,637 bases      Orientation:
plus strand
ORGUL member locations:
Legend (see complete legend)

ORGUL Member Locations for PLK1S1

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: KIZ_HUMAN, Q2M2Z5 (See protein sequence)
Recommended Name: Centrosomal protein kizuna  
Size: 673 amino acids; 75111 Da
Subunit: Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2
Subcellular location: Cytoplasm, cytoskeleton, centrosome. Note=Localizes to centrosomes throughout the cell cycle.
After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older
mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating
centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and
centrosome-surrounding area in a microtubule-independent and dependent manner, respectively
Miscellaneous: Kizuna means 'bonds' in Japanese
Sequence caution: Sequence=AAF67652.1; Type=Frameshift; Positions=283; Sequence=AAH39296.1; Type=Frameshift;
Positions=279; Sequence=AAP97689.1; Type=Frameshift; Positions=198; Sequence=BAG59066.1; Type=Erroneous initiation;
Secondary accessions: B4DDE9 B4DK54 Q4G0M8 Q4G0S5 Q5BKY3 Q6P0M6 Q71ME0 Q9NZ35
Alternative splicing: 5 isoforms:  Q2M2Z5-1   Q2M2Z5-2   Q2M2Z5-3   Q2M2Z5-4   Q2M2Z5-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for PLK1S1: NX_Q2M2Z5

Post-translational modifications:

  • Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but
  • is indispensable for spindle-pole stabilization1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q2M2Z5

  • PLK1S1 Protein expression data from MOPED and PaxDb:    About this image 
    PLK1S1 Protein Expression
    REFSEQ proteins (4 alternative transcripts): 
    NP_001156494.1  NP_001156495.1  NP_001263318.1  NP_060944.3  

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    Uscn Proteins for PLK1S1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--
    GO:0005813centrosome IDA16980960

    PLK1S1 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    PLK1S1 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR026742 Centrosomal_kizuma

    Graphical View of Domain Structure for InterPro Entry Q2M2Z5

    ProtoNet protein and cluster: Q2M2Z5

    UniProtKB/Swiss-Prot: KIZ_HUMAN, Q2M2Z5
    Similarity: Belongs to the kizuna family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: KIZ_HUMAN, Q2M2Z5
    Function: Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the
    forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar
    material around the centriole

         Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI19536135
    GO:0019901protein kinase binding IPI16980960
         
    PLK1S1 for ontologies           About GeneDecksing


    Phenotypes:
         2 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Plk1s1):
     no phenotypic analysis  normal 

    PLK1S1 for phenotypes           About GeneDecksing

    Animal Models:
         Mouse knock-out Plk1s1tm1.1Cpl for PLK1S1
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for PLK1S1 

    miRNA
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PLK1S1


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1PLK1 signaling events
    PLK1 signaling events1.00


    1 BioSystems Pathway for PLK1S1 
        PLK1 signaling events


    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for PLK1S1

    1 Interacting protein for PLK1S1 (Q2M2Z52, 3) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CEP72Q9P2092, 3MINT-7298066 MINT-7298084 MINT-7298075 I2D: score=1 
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007051spindle organization IMP16980960

    PLK1S1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for PLK1S1
    Search CenterWatch for drugs/clinical trials and news about PLK1S1 / KIZ 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for PLK1S1 gene (4 alternative transcripts): 
    NM_001163022.1  NM_001163023.1  NM_001276389.1  NM_018474.4  

    Unigene Cluster for PLK1S1:

    Polo-like kinase 1 substrate 1
    Hs.187635  [show with all ESTs]
    Unigene Representative Sequence: BC039296
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000445992(lincRNA) ENST00000246027(lincRNA)(uc002wsd.3) ENST00000457464(lincRNA) ENST00000447448(lincRNA)
    ENST00000428699(lincRNA) ENST00000451772(lincRNA) ENST00000419359(lincRNA) ENST00000441136(lincRNA) ENST00000423272(lincRNA)
    ENST00000427692(lincRNA)(uc002wsb.3 uc010zsh.2 uc010zsj.2) ENST00000424111(lincRNA)(uc010zsi.2)
    ENST00000537654(lincRNA) ENST00000432487(lincRNA) ENST00000458446(lincRNA)

    miRNA
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    Inhib. RNA
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    Additional cDNA sequence: 

    AF220187.1 AK025476.1 AK055105.1 AK293166.1 AK293567.1 AK295355.1 AK296399.1 AL110196.1 
    AL117539.1 BC013055.1 BC039296.1 BC045826.1 BC065550.1 BC090879.2 BC105093.1 BC113370.1 

    16 DOTS entries:

    DT.99980393  DT.316493  DT.443200  DT.91725291  DT.426076  DT.40273906  DT.95170490  DT.95170496 
    DT.65284295  DT.86856400  DT.86840982  DT.95170489  DT.100783717  DT.316488  DT.40109497  DT.91725273 

    24/196 AceView cDNA sequences (see all 196):

    AA994343 H81010 AK055105 AI686138 AI806322 BE047461 AV727256 AI380998 
    AI268635 BM983031 AI245979 BM978211 BE044560 AW663394 AI806668 BM979689 
    BM511019 BE350212 BQ007577 AA336975 Z45740 BF222104 AI823779 AI217950 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for PLK1S1    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13
    SP1:                                                                                            -               
    SP2:                                -                                                           -               
    SP3:                                                                                                            
    SP4:                                -     -                                                                     


    ECgene alternative splicing isoforms for PLK1S1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    PLK1S1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AAAACAAAAA
    PLK1S1 Expression
    About this image

    PLK1S1 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table

    2 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    Neural TubeDiencephalic Ventricular ZoneNeural Tube
    Neural TubeTelencephalonNeural Tube
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See PLK1S1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for PLK1S1

    SOURCE GeneReport for Unigene cluster: Hs.187635
        SABiosciences Custom PCR Arrays for PLK1S1
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PLK1S1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for PLK1S1 gene from 4/9 species (see all 9)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Plk1s11 , 5 polo-like kinase 1 substrate 11, 5 81.72(n)1
    70.35(a)1
      2 (72.63 cM)5
    2287301  NM_001033298.31  NP_001028470.11 
     1468558645 
    chicken
    (Gallus gallus)
    Aves PLK1S11 polo-like kinase 1 substrate 1 50.84(n)
    36.82(a)
      421237  NM_001006394.1  NP_001006394.1 
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.105882 Xenopus laevis transcribed sequences 80.72(n)    AW644298.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc:1625761 zgc:162576 42.05(n)
    29.77(a)
      567100  NM_001098390.1  NP_001091860.1 


    ENSEMBL Gene Tree for PLK1S1 (if available)
    TreeFam Gene Tree for PLK1S1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1982 NCBI SNPs in PLK1S1 are shown (see all 1982    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 20 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1426734211,2
    --21104818(+) CTGCTC/TAAAGG 3 -- us2k10--------
    rs1505796761,2
    --21104854(+) AAAAGG/TCCTCT 3 -- us2k10--------
    rs1395804561,2
    --21104855(+) AAAGGA/CCTCTT 3 -- us2k10--------
    rs1497131651,2
    --21104909(+) CTGCCC/TGGCCA 3 -- us2k10--------
    rs1831193061,2
    --21105076(+) AGAAGC/TAGGCA 3 -- us2k10--------
    rs778084381,2
    C--21105105(+) TTGTCC/TAGGTT 3 -- us2k12Minor allele frequency- T:0.09CSA WA 119
    rs1455300141,2
    --21105132(+) GGTCAA/GAGTGG 3 -- us2k10--------
    rs1882038071,2
    --21105307(+) CATCTC/GTCTTA 3 -- us2k10--------
    rs61324011,2
    C--21105377(+) AAGAAA/CCCAAA 3 -- us2k1 trp31Minor allele frequency- C:0.00NA 2
    rs800520071,2
    C--21105419(+) TACAGG/TCCGGG 3 -- us2k13Minor allele frequency- T:0.10CSA WA 120

    HapMap Linkage Disequilibrium report for PLK1S1 (21106624 - 21227260 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for PLK1S1
         1 CNV: 67668

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    PLK1S1 for disorders           About GeneDecksing

    2 diseases for PLK1S1:    About MalaCards
    pulmonary tuberculosis    tuberculosis


    Export disorders for PLK1S1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for PLK1S1 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with PLK1S1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cep72 regulates the localization of key centrosomal proteins and proper bipolar spindle formation. (PubMed id 19536135)1, 2 Oshimori N.... Yamamoto T. (2009)
    2. The Plk1 target Kizuna stabilizes mitotic centrosomes to ensure spindle bipolarity. (PubMed id 16980960)1, 2 Oshimori N.... Yamamoto T. (2006)
    3. The proteomic analysis of endogenous FAT10 substrates identifies p62/SQSTM1 as a substrate of FAT10ylation. (PubMed id 22797925)1 Aichem A....Groettrup M. (2012)
    4. A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)2 Dephoure N.... Gygi S.P. (2008)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
    6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
    7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    8. Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning. (PubMed id 10931946)2 Hu R.-M.... Chen J.-L. (2000)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 55857 HGNC: 15865 AceView: C20orf19 Ensembl:ENSG00000088970 euGenes: HUgn55857
    ECgene: PLK1S1 H-InvDB: PLK1S1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for PLK1S1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for PLK1S1 gene:
    Search GeneIP for patents involving PLK1S1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013 , 13 May 2013

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