PLBD1 Gene
protein-coding GIFtS : 45
GCID: GC12 M014657
phospholipase B domain containing 1
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Aliasesfor PLBD1 gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
and/or 10 fRNAdb )About This Section Aliases Phospholipase B Domain Containing 1 1 2 Phospholipase B-Like 12 Lamina Ancestor Homolog 12 3 PLB Homolog 12 Phospholipase B Domain-Containing Protein 12 3 Putative Phospholipase B-Like 12 LAMA-Like Protein 12 3 EC 3.1.1.-3 FLJ226621 EC 3.1.18
Export aliases for PLBD1 gene to outside databases Previous GC identifers: GC12M014548 GC12M014425
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Summariesfor PLBD1 gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section UniProtKB/Swiss-Prot: PLBL1_HUMAN, Q6P4A8 Function : Phospholipase acting on various phospholipids including phosphatidylcholine, phosphatidylinositol,phosphatidylethanolamine and lysophospholipids. May have a role in the defense against invading microorganisms and in the generation of lipid mediators of inflammation
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Genomic Viewsfor PLBD1 gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,
and/or miRBase ,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics )About This Section RefSeq DNA sequence: NC_000012.11 NC_018923.1 NT_009714.17 Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the PLBD1 gene promoter: c-Fos USF1 AP-1 SREBP-1c USF2 USF-1:USF-2 SREBP-1b SREBP-1a USF-1 c-Jun Other transcription factors Search SABiosciences Chromatin IP Primers for PLBD1 Epigenetics: QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat PLBD1
Genomic Location: Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 12p13.1 Ensembl cytogenetic band: 12p13.1 HGNC cytogenetic band: 12p13.1 PLBD1 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc information about chromosome 12 GeneLoc Exon Structure
GeneLoc location for GC12M014657: view genomic region
(about GC identifiers )
Start:
14,656,595 bp from pter
End:
14,721,283 bp from pter
Size:
64,689 bases
Orientation:
minus strand
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Proteinsfor PLBD1 gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
Biochemical Assays by
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: PLBL1_HUMAN, Q6P4A8 (See
protein sequence )Recommended Name: Phospholipase B-like 1 precursor Size : 553 amino acids; 63255 Da
Subunit : May exist as a non-covalently associated tetramer of two 22 kDa and two 42 kDa chains
Subcellular location : Cytoplasmic granule. Secreted
Sequence caution : Sequence=AAH00909.2; Type=Erroneous initiation; Sequence=BAB15442.1; Type=Erroneous initiation;
Secondary accessions : A8K4E9 Q9BVV3 Q9H625Explore the universe of human proteins at neXtProt for PLBD1: NX_Q6P4A8 Post-translational modifications:
Proteolytic processing leading to a 22 kDa N-terminal and a 42 kDa C-terminal fragment appears necessary for activity, which seems to derive from the 42 kDa chain1
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_Q6P4A8 4/5 DME Specific Peptides for PLBD1 (Q6P4A8 ) (see all 5 )PLBD1 Protein expression data from MOPED and PaxDb : About this image
REFSEQ proteins: NP_079105.4 ENSEMBL proteins: ENSP00000240617 ENSP00000441278 ENSP00000438367 Reactome Protein details: Q6P4A8 Human Recombinant Protein Products: Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view) : About this table
PLBD1 for ontologies About GeneDecksing PLBD1 Antibody Products: Assay Products for PLBD1:
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Protein
Domains / Familiesfor PLBD1 gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section
PLBD1 for domains About GeneDecksing 1 InterPro domain/family :
Graphical View of Domain Structure for InterPro Entry Q6P4A8 ProtoNet protein and cluster: Q6P4A8
UniProtKB/Swiss-Prot: PLBL1_HUMAN, Q6P4A8 Similarity : Belongs to the phospholipase B-like family
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Functionfor PLBD1 gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Function Summary: UniProtKB/Swiss-Prot: PLBL1_HUMAN, Q6P4A8 Function : Phospholipase acting on various phospholipids including phosphatidylcholine, phosphatidylinositol,phosphatidylethanolamine and lysophospholipids. May have a role in the defense against invading microorganisms and in the generation of lipid mediators of inflammation Biophysicochemical properties : pH dependence: Optimum pH is 7.4;Enzyme Numbers (IUBMB): EC 3.1.1 2 EC 3.1.1.- 1
Clone Products: Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for PLBD1 (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for PLBD1OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: PLBD1 (NM_024829 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PLBD1 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat PLBD1
In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PLBD1
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view) : About this table
GO ID Qualified GO term Evidence PubMed IDs GO:0016787 hydrolase activity
IEA --
PLBD1 for ontologies About GeneDecksing 1 GenomeRNAi human phenotype for PLBD1 :
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Pathways & Interactionsfor PLBD1 gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Unified GeneCards pathways   About this table See pathways by source Super-pathway contained gene-specific pathways 1 Acyl chain remodelling of PE 2 Glycerophospholipid biosynthesis 3 Metabolism
Pathway sources See GeneCards unified pathways Show all pathways 5/8
Reactome Pathways for PLBD1 (see all 8 )
PLBD1 for pathways About GeneDecksing Interactions: Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for PLBD1 STRING Interaction
Network Preview (showing 5 interactants - click image to see 8)5/8 Interacting proteins for PLBD1 (ENSP00000240617 4 ) via UniProtKB, MINT, STRING , and/or I2D (see all 8 )About this table Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7 ): About this table
GO ID Qualified GO term Evidence PubMed IDs GO:0006644 phospholipid metabolic process
TAS -- GO:0016042 lipid catabolic process
IEA -- GO:0036149 phosphatidylinositol acyl-chain remodeling
TAS -- GO:0036151 phosphatidylcholine acyl-chain remodeling
TAS -- GO:0036152 phosphatidylethanolamine acyl-chain remodeling
TAS --
PLBD1 for ontologies About GeneDecksing
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Drugs & Compoundsfor PLBD1 gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section Browse Tocris compounds for PLBD1 Search CenterWatch for drugs/clinical trials and news about PLBD1 / PLBL1
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Transcriptsfor PLBD1 gene (Secondary structures according to
fRNAdb ,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
shRNA from
OriGene ,
microRNA from QIAGEN ,
Tagged/untagged cDNA clones from
OriGene ,
SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for PLBD1 gene: NM_024829.5 Unigene Cluster for PLBD1:
Phospholipase B domain containing 1 Hs.131933 [show with all ESTs ] Unigene Representative Sequence: NM_024829 4 Ensembl transcripts including schematic representations, and UCSC links where relevant : ENST00000240617 (uc001rcc.1 ) ENST00000541800 ENST00000541618 ENST00000540572 Clone Products: OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for PLBD1 (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for PLBD1OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: PLBD1 (NM_024829 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PLBD1 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat PLBD1
Additional cDNA sequence: AK026315.1 AK225458.1 AK290914.1 BC000909.2 BC063561.1
7 DOTS entries : DT.215545 DT.121150828
DT.75101935 DT.91734534 DT.121150822 DT.75102752 DT.91817248 24/194 AceView cDNA sequences (see all 194 ):
BX643808 AW629861 AW169974 BG542885 BG572884 BU581527 BQ101253 CR602008 AA298290 AA513409 CR621086 BM263066 BU736309 CR615954 BM142639 AA252038 CR598292 AA931082 BG110795 AA669072 BX116782 AI025079 BX344111 BQ721063 GeneLoc Exon Structure
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Expression for PLBD1 gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,
PCR Arrays from
SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section PLBD1 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: AAACCAATTT
About this image PLBD1 expression in embryonic tissues and stem cells Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine About this table 1 LifeMap In Vivo Development Anatomical Compartment/Cell Tissue Anatomical Compartment
Cell Category (developmental path) Testis Seminiferous Tubules Sertoli cells Testis Expression: Positive Negative
Selective markerExperimental details:
Curated
Microarrays
In-situ hybridization
See PLBD1 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for PLBD1 SOURCE GeneReport for Unigene cluster: Hs.131933 UniProtKB/Swiss-Prot: PLBL1_HUMAN, Q6P4A8 Tissue specificity : Expressed in neutrophils and monocytes SABiosciences Custom PCR Arrays for PLBD1 Primer Products: OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for PLBD1Browse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human , mouse , rat PLBD1 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat PLBD1 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat PLBD1 In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PLBD1
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Orthologsfor PLBD1 gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section
This gene was present in the common ancestor of chordates.
Orthologs for PLBD1 gene from 3/15 species (see all 15 ) About this table
ENSEMBL Gene Tree for PLBD1 (if available)TreeFam Gene Tree for PLBD1 (if available)
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Paralogsfor PLBD1 gene (Paralogs according to
1 HomoloGene , 2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section Paralogs for PLBD1 gene PLBD2 2
PLBD1 for paralogs About GeneDecksing
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Genomic Variantsfor PLBD1 gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section
Genomic Data Transcription Related Data Allele Frequencies SNP ID Valid Clinical significance Chr 12 pos Sequence # AA Chg Type More # Allele freq Pop Total sample More
HapMap Linkage Disequilibrium report for PLBD1 (14656595 - 14721283 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV) variations for PLBD1: --
SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing PLBD1
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Disorders
/ Diseasesfor PLBD1 gene
(in which this Gene is Involved, According to MalaCards ,
OMIM, UniProtKB ,
the University of Copenhagen DISEASES
database ,
Genatlas , GeneTests ,
GAD ,
HuGE Navigator ,
and/or TGDB .)
About This Section --
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Publicationsfor PLBD1 gene (in
PubMed .
Associations of this gene to articles via
1 Entrez Gene ,
2 UniProtKB/Swiss-Prot ,
3 HGNC ,
4 GAD ,
5 PharmGKB ,
6 HMDB ,
7 DrugBank ,
8 UniProtKB/TrEMBL ,
9 Novoseek , and/or
10 fRNAdb )
About This Section PubMed articles for PLBD1 gene, integrated from 9 sources (see all 11 ): (articles sorted by number of sources associating them with PLBD1) Utopia : connect your pdf to the dynamic world of online information
The identification of a phospholipase B precursor in human neutrophils. (PubMed id 19019078) 1 , 2 , 3 Xu S....Venge P. (2009) Identification of phospholipase B from Dictyostelium discoideum reveals a new lipase family present in mammals, flies and nematodes, but not yeast. (PubMed id 15193148) 1 , 3 Morgan C.P....Cockcroft S. (2004) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932) 1 , 3 Strausberg R.L....Marra M.A. (2002) Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049) 1 OlA!h J....OvA!di J. (2011) Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048) 1 Danielsen J.M....Nielsen M.L. (2011) ErbB2-associated changes in the lysosomal proteome. (PubMed id 21674799) 1 Nylandsted J....Jaattela M. (2011) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. (PubMed id 19159218) 2 Chen R.... Zou H. (2009) The finished DNA sequence of human chromosome 12. (PubMed id 16541075) 2 Scherer S.E.... Gibbs R.A. (2006) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 2 Gerhard D.S....Malek J. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 2 Ota T.... Sugano S. (2004)
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External Searches for PLBD1 gene
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing PLBD1 gene
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
Kegg ,
and/or
H-InvDB )
About This Section
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Other Databases showing PLBD1 gene
(According to HUGE )
About This Section --
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Specialized Databases showing PLBD1 gene (According to PharmGKB ,
ATLAS , HORDE , IMGT , LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
Name Description
PharmGKB entry for PLBD1 Pharmacogenomics, SNPs, Pathways
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About This Section Patent Information for PLBD1 gene: Search GeneIP for patents involving PLBD1 GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Productsfor PLBD1 gene (Antibodies, recombinant proteins, and assays from EMD Millipore , R&D Systems , OriGene , QIAGEN , GenScript , Cell Signaling Technology , SABiosciences , Novus Biologicals , Sino Biological , Enzo Life Sciences , Abcam , ProSpec , Uscn , Thermo Fisher Scientific , Gene Editing from DNA2.0 , Clones from EMD Millipore , OriGene , GenScript , Sino Biological , DNA2.0 , SwitchGear Genomics , Vector BioLabs , Cell lines from GenScript and LifeMap BioReagents , PCR Arrays from SABiosciences , Drugs and/or compounds from EMD Millipore , Tocris Bioscience , and/or
Enzo Life Sciences ), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
Browse OriGene Antibodies OriGene shRNA RFP for PLBD1 OriGene 29mer shRNA kits in GFP-retroviral vector in human , mouse , rat for PLBD1 OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for PLBD1 OriGene Protein Over-expression Lysate for PLBD1 Browse OriGene Fluorogenic Cell Assay Kits OriGene siRNA for PLBD1 OriGene 3'-UTR Clone for PLBD1 OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for PLBD1 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for PLBD1 Browse OriGene GFP tagged cDNA clones in CMV expression vector Browse OriGene MicroRNA Expression Plasmids Browse OriGene basic RS shRNAs Browse OriGene validated miRNA SYBR primer pairs OriGene Purified Protein for PLBD1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling OriGene Custom Antibody Services for PLBD1 OriGene Custom Protein Services for PLBD1 OriGene Custom Immunoassay Development
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat PLBD1 QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing PLBD1 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat PLBD1 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human , mouse , rat PLBD1 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat PLBD1 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat PLBD1
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PLBD1 Proteins, Antibodies, CLIAs, and ELISAs
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PLBD1
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Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat PLBD1
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Aliases for PLBD1
Databases for PLBD1
Disorders / Diseases for PLBD1
Domains / Families for PLBD1
Drugs / Compounds for PLBD1
Expression for PLBD1
Function for PLBD1
Genomic Views for PLBD1
Intellectual Property for PLBD1
Orthologs for PLBD1
Paralogs for PLBD1
Pathways / Interactions for PLBD1
Products for PLBD1
Proteins for PLBD1
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Summaries for PLBD1
Transcripts for PLBD1
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