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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

PKN3 Gene

protein-coding   GIFtS: 57
GCID: GC09P131464

protein kinase N3

 Explore 2 diseases affiliated with
PKN3 via our new
 Human Malady Compendium 
Biological research products
for PKN3
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Protein Kinase N31 2     RP11-545E17.12
Protein Kinase PKN-Beta2 3     Serine/Threonine-Protein Kinase N32
Protein-Kinase C-Related Kinase 32 3     PKNBETA3
EC 2.7.11.133 8     EC 2.7.118
PKNbeta1     

External Ids:    HGNC: 179991   Entrez Gene: 299412   Ensembl: ENSG000001604477   OMIM: 6107145   UniProtKB: Q6P5Z23   

Export aliases for PKN3 gene to outside databases

Previous GC identifers: GC09P126841 GC09P128544 GC09P130504 GC09P101074


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: PKN3_HUMAN, Q6P5Z2
Function: Contributes to invasiveness in malignant prostate cancer

Gene Wiki entry for PKN3


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000009.11  NC_018920.1  NT_008470.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the PKN3 gene promoter:
         Max   Elk-1   Meis-1a   IRF-1   Meis-1b   Meis-1   c-Ets-1   c-Myc   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidPKN3 promoter sequence
   Search SABiosciences Chromatin IP Primers for PKN3

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PKN3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9q34.11   Ensembl cytogenetic band:  9q34.11   HGNC cytogenetic band: 9q34.13

PKN3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PKN3 gene location

GeneLoc information about chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09P131464:  view genomic region     (about GC identifiers)

Start:
131,464,802 bp from pter      End:
131,483,199 bp from pter
Size:
18,398 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: PKN3_HUMAN, Q6P5Z2 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase N3  
Size: 889 amino acids; 99421 Da
Subcellular location: Nucleus. Cytoplasm, perinuclear region. Note=Nuclear and perinuclear Golgi region
Secondary accessions: Q9UM03

Explore the universe of human proteins at neXtProt for PKN3: NX_Q6P5Z2

Post-translational modifications:

  • Autophosphorylated1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q6P5Z2

  • 4/14 DME Specific Peptides for PKN3 (Q6P5Z2) (see all 14)
     PVIERRP  GVLLYEM  LEDFLDN  RDLKLDN 

    PKN3 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_037487.2  
    ENSEMBL proteins: 
     ENSP00000291906  

    Human Recombinant Protein Products: 
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    Uscn Proteins for PKN3

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--
    GO:0005794Golgi apparatus TAS10441506
    GO:0048471perinuclear region of cytoplasm IEA--


    PKN3 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    PKN3 for domains           About GeneDecksing

    5/10 InterPro domains/families (see all 10):
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR017892 Pkinase_C
     IPR008271 Ser/Thr_kinase_AS

    Graphical View of Domain Structure for InterPro Entry Q6P5Z2

    ProtoNet protein and cluster: Q6P5Z2

    4 Blocks protein families:
    IPB000008 C2 domain
    IPB000861 PKN/rhophilin/rhotekin rho-binding repeat
    IPB000961 Protein kinase C-terminal domain
    IPB011072 Protein kinase PKN/PRK1


    UniProtKB/Swiss-Prot: PKN3_HUMAN, Q6P5Z2
    Domain: The C1 domain does not bind the diacylglycerol (DAG)
    Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily
    Similarity: Contains 1 AGC-kinase C-terminal domain
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 3 REM (Hr1) repeats


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: PKN3_HUMAN, Q6P5Z2
    Function: Contributes to invasiveness in malignant prostate cancer
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Two specific sites, Thr-718 (activation loop of the kinase domain) and Thr-860 (turn motif), need to
    be phosphorylated for its full activation (By similarity)

    Enzyme Numbers (IUBMB): EC 2.7.11.131 2 EC 2.7.112

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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity TAS10441506
    GO:0004697protein kinase C activity IEA--
    GO:0005515protein binding IPI11432776
    GO:0005524ATP binding IEA--


    PKN3 for ontologies           About GeneDecksing


    5 GenomeRNAi human phenotypes for PKN3:
     Decreased Hepatitis C virus re  Decreased substrate adherent c  Downregulation of NF-kappaB pa  Downregulation of NF-kappaB pa 
     Increased colony dispersion (i 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for PKN3

    STRING Interaction Network Preview (showing 4 interactants - click image to see more details)

    5 Interacting proteins for PKN3 (Q6P5Z21, 2, 3 ENSP000002919064) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ARHGAP26Q9UNA11, 3, ENSP000002744984EBI-1384335,EBI-1390913 I2D: score=3 STRING: ENSP00000274498
    PLD1Q133932, 3, ENSP000003427934MINT-15999 MINT-15998 MINT-15997 I2D: score=2 STRING: ENSP00000342793
    ARHGAP10A1A4S61, 3, ENSP000003369234STRING: ENSP00000336923 EBI-1384335,EBI-1390944 I2D: score=3 
    UBCENSP000003448184STRING: ENSP00000344818
    --Q220382MINT-14698 MINT-14697
    About this table

    Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006468protein phosphorylation TAS10441506
    GO:0007165signal transduction IEA--
    GO:0010631epithelial cell migration IDA--


    PKN3 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
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    Browse Tocris compounds for PKN3

    2 HMDB Compounds for PKN3    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    Search CenterWatch for drugs/clinical trials and news about PKN3 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for PKN3 gene: 
    NM_013355.3  

    Unigene Cluster for PKN3:

    Protein kinase N3
    Hs.300485  [show with all ESTs]
    Unigene Representative Sequence: NM_013355
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000291906(uc004bvw.3 uc010myh.3 uc022bom.1) ENST00000483521
    ENST00000485301

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    Additional cDNA sequence: 

    AB019692.1 BC023327.1 BC032794.1 BC041581.1 BC062558.1 

    5 DOTS entries:

    DT.99941669  DT.100679114  DT.100791012  DT.91965131  DT.100791008 

    24/114 AceView cDNA sequences (see all 114):

    AW245135 AW247208 BQ637451 BX365586 BC062558 BX362740 BC041581 CR614288 
    BQ718905 BU858997 AI741644 NM_013355 AI816759 BE964632 BC023327 BX112091 
    AW247261 AI813418 AW247872 AW452912 CB987960 AW028806 BG825922 BF512194 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for PKN3    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23
    SP1:                                                                                                                                                            
    SP2:                                                                                                              -                                             
    SP3:                                                                                                                                                            
    SP4:        -                                                                                                                                                   
    SP5:                                                                                                                                                            


    ECgene alternative splicing isoforms for PKN3

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    PKN3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGGCCACTGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    PKN3 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Definitive endoderm-like cells (A scalable, suspensi...)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See PKN3 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for PKN3

    SOURCE GeneReport for Unigene cluster: Hs.300485

    UniProtKB/Swiss-Prot: PKN3_HUMAN, Q6P5Z2
    Tissue specificity: Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PKN3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for PKN3 gene from 4/22 species (see all 22)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves PKN31 protein kinase N3 61.57(n)
    62.51(a)
      417209  XM_003642279.1  XP_003642327.1 
    zebrafish
    (Danio rerio)
    Actinopterygii pkn36
    protein kinase N3
    49(a)
    1 ↔ 1
    5(33468091-33494960)
    fruit fly
    (Drosophila melanogaster)
    Insecta Pkn6
    Protein kinase related to protein kinase N
    28(a)
    1 → many
    2R(5139815-5172602)
    worm
    (Caenorhabditis elegans)
    Secernentea pkn-16
    --
    27(a)
    1 → many
    X(10397183-10402516)


    ENSEMBL Gene Tree for PKN3 (if available)
    TreeFam Gene Tree for PKN3 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for PKN3 gene
    PRKCQ2  PRKCH2  PRKCB2  PRKCI2  PRKCE2  PKN12  PRKCA2  PRKCD2  
    PRKCZ2  PRKCG2  PKN22  
    18/35 SIMAP similar genes for PKN3 using alignment to 2 protein entries:     PKN3_HUMAN (see all proteins) (see all similar genes):
    KIN27    AKT1    PKN1    AKT3    MARK4    PRKACA
    PRKCD    PRKACB    PKN2    AKT2    PRKACG    PRKCZ
    PRKCH    PRKCI    RPS6KA3    PRKCG    RPS6KB1    PRKX

    PKN3 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/13 NCBI SNPs in PKN3 are shown (see all 13    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 9 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs671438721,2
    C,--131464629(+) GAGAC-/AAAGAGA 1 -- us2k10--------
    rs556979361,2
    C,--131471577(+) AAAAAT/-AATAA 1 -- int11Minor allele frequency- -:0.00NA 2
    rs1841151281,2
    --131472098(+) TACTCA/GGGAGG 1 -- int10--------
    rs1885069761,2
    --131472267(+) TGGCGC/TGATCT 1 -- int10--------
    rs343403691,2
    C,--131474198(+) GTTTG-/TTTTTT 1 -- int11Minor allele frequency- T:0.00NA 2
    rs105471231,2
    C,--131481176(+) ttttt-/GGtttgt 1 -- int10--------
    rs2017456051,2
    --131481180(+) TGGTT-/TGTTTTT 1 -- int10--------
    rs570923041,2
    C--131481181(+) GGTTTG/TTTTTT 1 -- int10--------
    rs1997605971,2
    --131481181(+) GGTTT-/GTTTTT 1 -- int10--------
    rs1118780681,2
    ----131464630(+) AGACAA/GAGAGA 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for PKN3 (131464802 - 131483199 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for PKN3
         1 CNV: 6716
    Human Gene Mutation Database (HGMD): PKN3

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PKN3
    DNA2.0 Custom Variant and Variant Library Synthesis for PKN3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    PKN3 for disorders           About GeneDecksing

    OMIM gene information: 610714    OMIM disorders: --

    2 diseases for PKN3:    About MalaCards
    prostate cancer    prostatitis


    Export disorders for PKN3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for PKN3 gene, integrated from 9 sources (see all 17):
    (articles sorted by number of sources associating them with PKN3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha. (PubMed id 10441506)1, 2, 3, 9 Oishi K.... Ono Y. (1999)
    2. DNA sequence and analysis of human chromosome 9. (PubMed id 15164053)1, 2 Humphray S.J.... Dunham I. (2004)
    3. PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase. (PubMed id 15282551)1, 2 Leenders F.... Klippel A. (2004)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    5. PKN regulates phospholipase D1 through direct interaction. (PubMed id 11259428)1, 9 Oishi K....Ono Y. (2001)
    6. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    7. Regulatory domain selectivity in the cell-type specific PKN- dependence of cell migration. (PubMed id 21754995)1 Lachmann S.... Parker P.J. (2011)
    8. Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)1 Lee K.A....Doedens J.R. (2011)
    9. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    10. Large-scale proteomics analysis of the human kinome. (PubMed id 19369195)2 Oppermann F.S.... Daub H. (2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 29941 HGNC: 17999 AceView: PKN3 Ensembl:ENSG00000160447 euGenes: HUgn29941
    ECgene: PKN3 H-InvDB: PKN3

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for PKN3 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for PKN3 gene:
    Search GeneIP for patents involving PKN3

    GeneCards and IP:
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