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Aliases for PKN1 Gene

Aliases for PKN1 Gene

  • Protein Kinase N1 2 3 5
  • Protein Kinase C-Like 1 2 3 4
  • Protein-Kinase C-Related Kinase 1 3 4
  • Serine-Threonine Protein Kinase N 3 4
  • Protease-Activated Kinase 1 3 4
  • Protein Kinase C-Like PKN 3 4
  • Protein Kinase PKN-Alpha 3 4
  • EC 2.7.11.13 4 63
  • PRKCL1 3 4
  • PAK-1 3 4
  • PAK1 3 4
  • PRK1 3 4
  • PKN 3 4
  • Protein Kinase C-Related Kinase 1 3
  • Serine/Threonine Protein Kinase N 3
  • Serine-Threonine Kinase N 3
  • PKN-ALPHA 3
  • EC 2.7.11 63
  • DBK 3

External Ids for PKN1 Gene

Previous HGNC Symbols for PKN1 Gene

  • PRKCL1

Previous GeneCards Identifiers for PKN1 Gene

  • GC19P014400
  • GC19P014544
  • GC19P014121

Summaries for PKN1 Gene

Entrez Gene Summary for PKN1 Gene

  • The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

GeneCards Summary for PKN1 Gene

PKN1 (Protein Kinase N1) is a Protein Coding gene. Diseases associated with PKN1 include paraneoplastic cerebellar degeneration. Among its related pathways are PI3K-Akt signaling pathway and Salmonella infection (KEGG). GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKCB.

UniProtKB/Swiss-Prot for PKN1 Gene

  • PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates Ser-575, Ser-637 and Ser-669 of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of Thr-11 of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 Lys-9 (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates Thr-38 of PPP1R14A, Ser-159, Ser-163 and Ser-170 of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.

Gene Wiki entry for PKN1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PKN1 Gene

Genomics for PKN1 Gene

Regulatory Elements for PKN1 Gene

Enhancers for PKN1 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around PKN1 on UCSC Golden Path with GeneCards custom track

Promoters for PKN1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around PKN1 on UCSC Golden Path with GeneCards custom track

Genomic Location for PKN1 Gene

Chromosome:
19
Start:
14,433,053 bp from pter
End:
14,471,867 bp from pter
Size:
38,815 bases
Orientation:
Plus strand

Genomic View for PKN1 Gene

Genes around PKN1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PKN1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PKN1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PKN1 Gene

Proteins for PKN1 Gene

  • Protein details for PKN1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q16512-PKN1_HUMAN
    Recommended name:
    Serine/threonine-protein kinase N1
    Protein Accession:
    Q16512
    Secondary Accessions:
    • A8K7W5
    • B2R9R4
    • B3KVN3
    • Q15143
    • Q504U4
    • Q8IUV5
    • Q9UD44

    Protein attributes for PKN1 Gene

    Size:
    942 amino acids
    Molecular mass:
    103932 Da
    Quaternary structure:
    • Interacts with ZFAND6 (By similarity). Interacts with ANDR (PubMed:12514133). Interacts with PRKCB (PubMed:20228790). Interacts (via REM 1 and REM 2 repeats) with RAC1 (PubMed:14514689, PubMed:18006505). Interacts (via REM 1 repeat) with RHOA (PubMed:10619026, PubMed:8571126). Interacts with RHOB (PubMed:9478917). Interacts (via C-terminus) with PDPK1 (PubMed:10792047). Interacts with CCNT2; enhances MYOD1-dependent transcription (PubMed:16331689).
    • (Microbial infection) Interacts (via the second REM repeat) with S.typhimurium E3 ubiquitin-protein ligase SspH1 (via the leucine-rich repeat region) (PubMed:16611232, PubMed:24248594).

    Three dimensional structures from OCA and Proteopedia for PKN1 Gene

    Alternative splice isoforms for PKN1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PKN1 Gene

Proteomics data for PKN1 Gene at MOPED

Post-translational modifications for PKN1 Gene

  • (Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.
  • Activated by limited proteolysis with trypsin.
  • Autophosphorylated; preferably on serine. Phosphorylated during mitosis.
  • Ubiquitination at Lys 263
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PKN1 (PKN1)

Domains & Families for PKN1 Gene

Suggested Antigen Peptide Sequences for PKN1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q16512

UniProtKB/Swiss-Prot:

PKN1_HUMAN :
  • The C1 domain does not bind the diacylglycerol (DAG).
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  • Contains 3 REM (Hr1) repeats.
Domain:
  • The C1 domain does not bind the diacylglycerol (DAG).
  • Contains 1 AGC-kinase C-terminal domain.
  • Contains 1 C2 domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Similarity:
  • Contains 3 REM (Hr1) repeats.
genes like me logo Genes that share domains with PKN1: view

Function for PKN1 Gene

Molecular function for PKN1 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=20.6 uM for HDAC5 {ECO:0000269 PubMed:20188095};
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Kinase activity is activated upon binding to Rho proteins (RHOA, RHOB and RAC1). Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by caspase-3 (CASP3) cleavage during apoptosis. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.
UniProtKB/Swiss-Prot Function:
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates Ser-575, Ser-637 and Ser-669 of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of Thr-11 of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 Lys-9 (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates Thr-38 of PPP1R14A, Ser-159, Ser-163 and Ser-170 of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.

Enzyme Numbers (IUBMB) for PKN1 Gene

Gene Ontology (GO) - Molecular Function for PKN1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004697 protein kinase C activity IEA --
GO:0005080 protein kinase C binding IPI 20228790
GO:0035402 histone kinase activity (H3-T11 specific) IDA 18066052
GO:0050681 androgen receptor binding IDA 12514133
genes like me logo Genes that share ontologies with PKN1: view
genes like me logo Genes that share phenotypes with PKN1: view

Animal Models for PKN1 Gene

MGI Knock Outs for PKN1:

Animal Model Products

  • Taconic Biosciences Mouse Models for PKN1

miRNA for PKN1 Gene

miRTarBase miRNAs that target PKN1

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PKN1 Gene

Localization for PKN1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PKN1 Gene

Cytoplasm. Nucleus. Endosome. Cell membrane; Peripheral membrane protein. Cleavage furrow. Midbody. Note=Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. {ECO:0000250 UniProtKB:Q63433, ECO:0000269 PubMed:17332740}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PKN1 Gene COMPARTMENTS Subcellular localization image for PKN1 gene
Compartment Confidence
cytosol 5
endosome 5
nucleus 5
plasma membrane 3
cytoskeleton 2

Gene Ontology (GO) - Cellular Components for PKN1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0043234 protein complex IEA --
genes like me logo Genes that share ontologies with PKN1: view

Pathways & Interactions for PKN1 Gene

genes like me logo Genes that share pathways with PKN1: view

SIGNOR curated interactions for PKN1 Gene

Activates:
Inactivates:
Is activated by:

Gene Ontology (GO) - Biological Process for PKN1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001782 B cell homeostasis IEA --
GO:0001933 negative regulation of protein phosphorylation IEA --
GO:0002634 regulation of germinal center formation IEA --
GO:0006351 transcription, DNA-templated IEA --
GO:0006468 protein phosphorylation IEA,IDA 17332740
genes like me logo Genes that share ontologies with PKN1: view

Drugs & Compounds for PKN1 Gene

(14) Drugs for PKN1 Gene - From: ApexBio, DGIdb, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Ingenol Mebutate Approved Pharma 0
Adenosine triphosphate Approved Nutra 0
[Ser25] Protein Kinase C (19-31) Pharma PKC substrate 0
sophoretin Pharma inhibitor Kinase Inhibitors 0

(8) Additional Compounds for PKN1 Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0

(1) ApexBio Compounds for PKN1 Gene

Compound Action Cas Number
[Ser25] Protein Kinase C (19-31) PKC substrate 136795-05-6
genes like me logo Genes that share compounds with PKN1: view

Transcripts for PKN1 Gene

Unigene Clusters for PKN1 Gene

Protein kinase N1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for PKN1 Gene

ExUns: 1 ^ 2 ^ 3a · 3b · 3c · 3d ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c · 8d ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b ^ 15a · 15b ^ 16 ^ 17a ·
SP1: - - - -
SP2: - -
SP3: - - - -
SP4:
SP5: - -
SP6: - - - - - - - -
SP7: - - - - - - -
SP8: - -
SP9:
SP10: - - -
SP11:
SP12:

ExUns: 17b ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23a · 23b · 23c ^ 24a · 24b ^ 25a · 25b ^ 26 ^ 27
SP1: - -
SP2: - -
SP3: -
SP4: - -
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11: -
SP12:

Relevant External Links for PKN1 Gene

GeneLoc Exon Structure for
PKN1
ECgene alternative splicing isoforms for
PKN1

Expression for PKN1 Gene

mRNA expression in normal human tissues for PKN1 Gene

Protein differential expression in normal tissues from HIPED for PKN1 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (14.9) and Lymph node (13.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for PKN1 Gene



SOURCE GeneReport for Unigene cluster for PKN1 Gene Hs.466044

mRNA Expression by UniProt/SwissProt for PKN1 Gene

Q16512-PKN1_HUMAN
Tissue specificity: Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
genes like me logo Genes that share expression patterns with PKN1: view

Protein tissue co-expression partners for PKN1 Gene

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for PKN1 Gene

Orthologs for PKN1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PKN1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia PKN1 35
  • 90.2 (n)
  • 95.65 (a)
PKN1 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PKN1 35
  • 89.77 (n)
  • 94.69 (a)
PKN1 36
  • 78 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Pkn1 35
  • 85.48 (n)
  • 92.14 (a)
Pkn1 16
Pkn1 36
  • 91 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia PKN1 35
  • 98.41 (n)
  • 98.62 (a)
PKN1 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Pkn1 35
  • 85.58 (n)
  • 92.45 (a)
oppossum
(Monodelphis domestica)
Mammalia PKN1 36
  • 80 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PKN1 36
  • 80 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PKN1 36
  • 68 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.15460 35
zebrafish
(Danio rerio)
Actinopterygii Dr.16985 35
LOC560840 35
  • 65.79 (n)
  • 69.22 (a)
pkn1 36
  • 66 (a)
OneToMany
PKN1 (2 of 2) 36
  • 62 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Pkn 36
  • 34 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea pkn-1 36
  • 33 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes PKC1 36
  • 23 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 39 (a)
OneToMany
Species with no ortholog for PKN1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PKN1 Gene

ENSEMBL:
Gene Tree for PKN1 (if available)
TreeFam:
Gene Tree for PKN1 (if available)

Paralogs for PKN1 Gene

Paralogs for PKN1 Gene

genes like me logo Genes that share paralogs with PKN1: view

Variants for PKN1 Gene

Sequence variations from dbSNP and Humsavar for PKN1 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
rs10846 - 14,471,656(+) CCGAC(A/G)TCAGC reference, missense
VAR_042337 A metastatic melanoma sample
VAR_042338 -
rs35132656 - 14,458,262(+) TCTGG(C/T)GGGAC reference, missense
rs56273055 - 14,463,891(+) GCTCC(A/G)GAGGC reference, missense

Structural Variations from Database of Genomic Variants (DGV) for PKN1 Gene

Variant ID Type Subtype PubMed ID
nsv833764 CNV Loss 17160897
nsv911121 CNV Loss 21882294
nsv911122 CNV Loss 21882294
nsv911123 CNV Loss 21882294
dgv3745n71 CNV Loss 21882294
nsv470127 CNV Loss 18288195
nsv911126 CNV Gain 21882294
nsv828456 CNV Loss 20364138
nsv828457 CNV Gain 20364138
nsv911127 CNV Loss 21882294
nsv817814 CNV Loss 17921354
nsv527867 CNV Loss 19592680

Variation tolerance for PKN1 Gene

Residual Variation Intolerance Score: 57.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 10.62; 91.32% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PKN1 Gene

HapMap Linkage Disequilibrium report
PKN1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PKN1 Gene

Disorders for PKN1 Gene

MalaCards: The human disease database

(1) MalaCards diseases for PKN1 Gene - From: GeneCards

Disorder Aliases PubMed IDs
paraneoplastic cerebellar degeneration
- elite association - COSMIC cancer census association via MalaCards
Search PKN1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for PKN1

Genetic Association Database (GAD)
PKN1
Human Genome Epidemiology (HuGE) Navigator
PKN1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PKN1
genes like me logo Genes that share disorders with PKN1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PKN1 Gene

Publications for PKN1 Gene

  1. Molecular dissection of the interaction between the small G proteins Rac1 and RhoA and protein kinase C-related kinase 1 (PRK1). (PMID: 14514689) Owen D. … Mott H.R. (J. Biol. Chem. 2003) 3 4 23 67
  2. The protein kinase N (PKN) gene PRKCL1/Prkcl1 maps to human chromosome 19p12-p13.1 and mouse chromosome 8 with close linkage to the myodystrophy (myd) mutation. (PMID: 9570957) Bartsch J.W. … Ono Y. (Genomics 1998) 2 3 23
  3. Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases. (PMID: 20188095) Harrison B.C. … McKinsey T.A. (FEBS Lett. 2010) 3 23
  4. PRK1 distribution in normal tissues and carcinomas: overexpression and activation in ovarian serous carcinoma. (PMID: 19427017) Galgano M.T. … Frierson H.F. (Hum. Pathol. 2009) 3 23
  5. The Rac1 polybasic region is required for interaction with its effector PRK1. (PMID: 18006505) Modha R. … Mott H.R. (J. Biol. Chem. 2008) 3 23

Products for PKN1 Gene

Sources for PKN1 Gene

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