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Aliases for PIWIL4 Gene

Aliases for PIWIL4 Gene

  • Piwi Like RNA-Mediated Gene Silencing 4 2 3
  • Piwi-Like RNA-Mediated Gene Silencing 4 3 5
  • HIWI2 3 4
  • Testis Tissue Sperm-Binding Protein Li 85P 3
  • Piwi-Like 4 (Drosophila) 2
  • Piwi-Like 4 3
  • MIWI2 3
  • PIWI 4

External Ids for PIWIL4 Gene

Previous GeneCards Identifiers for PIWIL4 Gene

  • GC00U991201
  • GC11P093989
  • GC11P093940
  • GC11P094300
  • GC11P090365
  • GC11P094278

Summaries for PIWIL4 Gene

Entrez Gene Summary for PIWIL4 Gene

  • PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]

GeneCards Summary for PIWIL4 Gene

PIWIL4 (Piwi Like RNA-Mediated Gene Silencing 4) is a Protein Coding gene. Diseases associated with PIWIL4 include klatskin's tumor. Among its related pathways are Gene Expression and Mitotic Prophase. GO annotations related to this gene include nucleic acid binding and piRNA binding. An important paralog of this gene is AGO3.

UniProtKB/Swiss-Prot for PIWIL4 Gene

  • Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing Lys-9 methylation of histone H3 at some loci (PubMed:12906857).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PIWIL4 Gene

Genomics for PIWIL4 Gene

Regulatory Elements for PIWIL4 Gene

Promoters for PIWIL4 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around PIWIL4 on UCSC Golden Path with GeneCards custom track

Genomic Location for PIWIL4 Gene

Chromosome:
11
Start:
94,543,840 bp from pter
End:
94,621,421 bp from pter
Size:
77,582 bases
Orientation:
Plus strand

Genomic View for PIWIL4 Gene

Genes around PIWIL4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PIWIL4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PIWIL4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PIWIL4 Gene

Proteins for PIWIL4 Gene

  • Protein details for PIWIL4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q7Z3Z4-PIWL4_HUMAN
    Recommended name:
    Piwi-like protein 4
    Protein Accession:
    Q7Z3Z4
    Secondary Accessions:
    • B4DEG5
    • Q68CZ4
    • Q8N8G9
    • Q8N9V8
    • Q8NEH2

    Protein attributes for PIWIL4 Gene

    Size:
    852 amino acids
    Molecular mass:
    96589 Da
    Quaternary structure:
    • Interacts with PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2 and TDRD9. Interacts with MOV10L1.
    SequenceCaution:
    • Sequence=BAC04873.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAH18436.1; Type=Frameshift; Positions=454; Evidence={ECO:0000305};

    Alternative splice isoforms for PIWIL4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PIWIL4 Gene

Proteomics data for PIWIL4 Gene at MOPED

Post-translational modifications for PIWIL4 Gene

  • Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • Ubiquitination at Lys 676 and Lys 679
  • Modification sites at PhosphoSitePlus

Other Protein References for PIWIL4 Gene

Antibody Products

No data available for DME Specific Peptides for PIWIL4 Gene

Domains & Families for PIWIL4 Gene

Gene Families for PIWIL4 Gene

Suggested Antigen Peptide Sequences for PIWIL4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q7Z3Z4

UniProtKB/Swiss-Prot:

PIWL4_HUMAN :
  • Contains 1 PAZ domain.
  • Belongs to the argonaute family. Piwi subfamily.
Domain:
  • Contains 1 PAZ domain.
  • Contains 1 Piwi domain.
Family:
  • Belongs to the argonaute family. Piwi subfamily.
genes like me logo Genes that share domains with PIWIL4: view

Function for PIWIL4 Gene

Molecular function for PIWIL4 Gene

UniProtKB/Swiss-Prot Function:
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing Lys-9 methylation of histone H3 at some loci (PubMed:12906857).
genes like me logo Genes that share phenotypes with PIWIL4: view

Animal Models for PIWIL4 Gene

MGI Knock Outs for PIWIL4:

Animal Model Products

miRNA for PIWIL4 Gene

miRTarBase miRNAs that target PIWIL4

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PIWIL4 Gene

Localization for PIWIL4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PIWIL4 Gene

Nucleus. Cytoplasm. Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity). {ECO:0000250 UniProtKB:Q8CGT6}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PIWIL4 Gene COMPARTMENTS Subcellular localization image for PIWIL4 gene
Compartment Confidence
nucleus 5
cytosol 2
mitochondrion 1

Gene Ontology (GO) - Cellular Components for PIWIL4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm ISS --
genes like me logo Genes that share ontologies with PIWIL4: view

Pathways & Interactions for PIWIL4 Gene

genes like me logo Genes that share pathways with PIWIL4: view

Pathways by source for PIWIL4 Gene

1 KEGG pathway for PIWIL4 Gene

Gene Ontology (GO) - Biological Process for PIWIL4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006417 regulation of translation IEA --
GO:0007275 multicellular organism development IEA --
GO:0007283 spermatogenesis ISS --
GO:0031047 gene silencing by RNA ISS,TAS --
GO:0043046 DNA methylation involved in gamete generation ISS --
genes like me logo Genes that share ontologies with PIWIL4: view

No data available for SIGNOR curated interactions for PIWIL4 Gene

Drugs & Compounds for PIWIL4 Gene

No Compound Related Data Available

Transcripts for PIWIL4 Gene

Unigene Clusters for PIWIL4 Gene

Piwi-like RNA-mediated gene silencing 4:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for PIWIL4 Gene

No ASD Table

Relevant External Links for PIWIL4 Gene

GeneLoc Exon Structure for
PIWIL4
ECgene alternative splicing isoforms for
PIWIL4

Expression for PIWIL4 Gene

mRNA expression in normal human tissues for PIWIL4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for PIWIL4 Gene

This gene is overexpressed in Testis (31.8), Monocytes (23.7), and Ovary (13.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for PIWIL4 Gene



SOURCE GeneReport for Unigene cluster for PIWIL4 Gene Hs.660188

mRNA Expression by UniProt/SwissProt for PIWIL4 Gene

Q7Z3Z4-PIWL4_HUMAN
Tissue specificity: Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
genes like me logo Genes that share expression patterns with PIWIL4: view

Protein tissue co-expression partners for PIWIL4 Gene

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for PIWIL4 Gene

Orthologs for PIWIL4 Gene

This gene was present in the common ancestor of animals.

Orthologs for PIWIL4 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia PIWIL4 35
  • 84.75 (n)
  • 81.65 (a)
PIWIL4 36
  • 81 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PIWIL4 35
  • 85.41 (n)
  • 82.61 (a)
PIWIL4 36
  • 82 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Piwil4 35
  • 76.98 (n)
  • 75.16 (a)
Piwil4 16
Piwil4 36
  • 67 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia PIWIL4 35
  • 99.53 (n)
  • 99.41 (a)
PIWIL4 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Piwil4 35
  • 78.94 (n)
  • 78.34 (a)
oppossum
(Monodelphis domestica)
Mammalia PIWIL4 36
  • 70 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PIWIL4 36
  • 56 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia piwil4 35
  • 63.56 (n)
  • 60.67 (a)
fruit fly
(Drosophila melanogaster)
Insecta AGO3 36
  • 34 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea ergo-1 36
  • 14 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 42 (a)
OneToMany
Species with no ortholog for PIWIL4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for PIWIL4 Gene

ENSEMBL:
Gene Tree for PIWIL4 (if available)
TreeFam:
Gene Tree for PIWIL4 (if available)

Paralogs for PIWIL4 Gene

Paralogs for PIWIL4 Gene

(3) SIMAP similar genes for PIWIL4 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with PIWIL4: view

Variants for PIWIL4 Gene

Sequence variations from dbSNP and Humsavar for PIWIL4 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type
rs11020845 - 94,589,186(+) CTTTC(A/T)GAAGC reference, missense
rs12276921 - 94,575,065(+) CAAAC(A/G)GGACT reference, missense
rs12272255 - 94,577,479(+) ACAAA(A/G)GCTAG reference, missense
rs57607909 - 94,593,599(+) GTGAG(C/G)CTCAG reference, missense
rs476209 -- 94,599,565(-) GTCAA(A/C)GTTCA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PIWIL4 Gene

Variant ID Type Subtype PubMed ID
esv2671948 CNV Deletion 23128226

Variation tolerance for PIWIL4 Gene

Residual Variation Intolerance Score: 53.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 7.83; 83.54% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PIWIL4 Gene

HapMap Linkage Disequilibrium report
PIWIL4
Human Gene Mutation Database (HGMD)
PIWIL4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PIWIL4 Gene

Disorders for PIWIL4 Gene

MalaCards: The human disease database

(1) MalaCards diseases for PIWIL4 Gene - From: DISEASES

Disorder Aliases PubMed IDs
klatskin's tumor
  • hilar cholangiocarcinoma
- elite association - COSMIC cancer census association via MalaCards

Relevant External Links for PIWIL4

Genetic Association Database (GAD)
PIWIL4
Human Genome Epidemiology (HuGE) Navigator
PIWIL4
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PIWIL4
genes like me logo Genes that share disorders with PIWIL4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PIWIL4 Gene

Publications for PIWIL4 Gene

  1. Identification of eight members of the Argonaute family in the human genome. (PMID: 12906857) Sasaki T. … Shimizu N. (Genomics 2003) 2 3 4 67
  2. Conditional inactivation of Miwi2 reveals that MIWI2 is only essential for prospermatogonial development in mice. (PMID: 24464225) Bao J. … Yan W. (Cell Death Differ. 2014) 3
  3. Piwi-like 1 and 4 gene transcript levels are associated with clinicopathological parameters in renal cell carcinomas. (PMID: 24509249) Al-Janabi O. … Taubert H. (Biochim. Biophys. Acta 2014) 3
  4. Association of PIWIL4 genetic variants with germ cell maturation arrest in infertile Spanish men. (PMID: 24969058) MuA+oz X. … Larriba S. (Asian J. Androl. 2014) 3
  5. The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic cells. (PMID: 25038252) Keam S.P. … Suter C.M. (Nucleic Acids Res. 2014) 3

Products for PIWIL4 Gene

Sources for PIWIL4 Gene

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