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Aliases for PIK3R4 Gene

Aliases for PIK3R4 Gene

  • Phosphoinositide-3-Kinase, Regulatory Subunit 4 2 3
  • Phosphoinositide 3-Kinase Adaptor Protein 3 4
  • PI3-Kinase Regulatory Subunit 4 3 4
  • PI3-Kinase P150 Subunit 3 4
  • EC 2.7.11.1 4 63
  • VPS15 3 4
  • Phosphoinositide-3-Kinase, Regulatory Subunit 4, P150 3
  • Phosphatidylinositol 3-Kinase-Associated P150 3
  • P150 3

External Ids for PIK3R4 Gene

Previous GeneCards Identifiers for PIK3R4 Gene

  • GC03P127093
  • GC03M131114
  • GC03M131679
  • GC03M131718
  • GC03M131630
  • GC03M131880
  • GC03M130397
  • GC03M127782

Summaries for PIK3R4 Gene

GeneCards Summary for PIK3R4 Gene

PIK3R4 (Phosphoinositide-3-Kinase, Regulatory Subunit 4) is a Protein Coding gene. Among its related pathways are Immune System and Signaling by GPCR. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and binding.

UniProtKB/Swiss-Prot for PIK3R4 Gene

  • Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).

Tocris Summary for PIK3R4 Gene

  • PI 3-Kinases (phosphoinositide 3-kinases, PI 3-Ks) are a family of lipid kinases capable of phosphorylating the 3'OH of the inositol ring of phosphoinositides. They are responsible for coordinating a diverse range of cell functions including proliferation and survival.

Gene Wiki entry for PIK3R4 Gene

No data available for Entrez Gene Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PIK3R4 Gene

Genomics for PIK3R4 Gene

Regulatory Elements for PIK3R4 Gene

Genomic Location for PIK3R4 Gene

Chromosome:
3
Start:
130,678,934 bp from pter
End:
130,746,852 bp from pter
Size:
67,919 bases
Orientation:
Minus strand

Genomic View for PIK3R4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PIK3R4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PIK3R4 Gene

Proteins for PIK3R4 Gene

  • Protein details for PIK3R4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99570-PI3R4_HUMAN
    Recommended name:
    Phosphoinositide 3-kinase regulatory subunit 4
    Protein Accession:
    Q99570
    Secondary Accessions:
    • Q2TBF4

    Protein attributes for PIK3R4 Gene

    Size:
    1358 amino acids
    Molecular mass:
    153103 Da
    Cofactor:
    Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
    Quaternary structure:
    • Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG (PubMed:8999962, PubMed:19270696, PubMed:23878393, PubMed:25490155). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (PubMed:25490155). Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as KIAA0226/Rubicon, SH3GLB1/Bif-1, AMBRA1 and NRBF2 (PubMed:19270696, PubMed:20643123, PubMed:24785657). PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3C3/VPS34 (PubMed:14617358). Interacts with NRBF2 (PubMed:24785657).

neXtProt entry for PIK3R4 Gene

Proteomics data for PIK3R4 Gene at MOPED

Post-translational modifications for PIK3R4 Gene

Other Protein References for PIK3R4 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PIK3R4 (PIK3R4)

Domains & Families for PIK3R4 Gene

Suggested Antigen Peptide Sequences for PIK3R4 Gene

Graphical View of Domain Structure for InterPro Entry

Q99570

UniProtKB/Swiss-Prot:

PI3R4_HUMAN :
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
  • Contains 3 HEAT repeats.
Domain:
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Similarity:
  • Contains 3 HEAT repeats.
  • Contains 7 WD repeats.
genes like me logo Genes that share domains with PIK3R4: view

Function for PIK3R4 Gene

Molecular function for PIK3R4 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot Function:
Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).

Enzyme Numbers (IUBMB) for PIK3R4 Gene

Gene Ontology (GO) - Molecular Function for PIK3R4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity NAS 8999962
GO:0004674 protein serine/threonine kinase activity IBA --
GO:0005488 binding --
GO:0005515 protein binding IPI 14617358
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with PIK3R4: view
genes like me logo Genes that share phenotypes with PIK3R4: view

Animal Models for PIK3R4 Gene

MGI Knock Outs for PIK3R4:

Animal Model Products

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PIK3R4

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for PIK3R4 Gene

Localization for PIK3R4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PIK3R4 Gene

Late endosome. Cytoplasmic vesicle, autophagosome. Note=As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (By similarity). {ECO:0000250 UniProtKB:Q8VD65, ECO:0000305}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PIK3R4 Gene COMPARTMENTS Subcellular localization image for PIK3R4 gene
Compartment Confidence
endosome 5
cytosol 4
nucleus 3
vacuole 3
cytoskeleton 2
lysosome 2
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for PIK3R4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005643 nuclear pore IBA --
GO:0005770 late endosome IDA 14617358
GO:0005776 autophagosome IEA --
GO:0005829 cytosol TAS --
GO:0005930 axoneme ISS --
genes like me logo Genes that share ontologies with PIK3R4: view

Pathways & Interactions for PIK3R4 Gene

genes like me logo Genes that share pathways with PIK3R4: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

SIGNOR curated interactions for PIK3R4 Gene

Activates:

Gene Ontology (GO) - Biological Process for PIK3R4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002224 toll-like receptor signaling pathway TAS --
GO:0006468 protein phosphorylation NAS 8999962
GO:0006623 protein targeting to vacuole IBA --
GO:0006644 phospholipid metabolic process TAS --
GO:0006661 phosphatidylinositol biosynthetic process TAS --
genes like me logo Genes that share ontologies with PIK3R4: view

Drugs & Compounds for PIK3R4 Gene

(25) Drugs for PIK3R4 Gene - From: DGIdb, HMDB, and Tocris

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
3-Methyladenine Experimental Pharma Class III PI3K inhibitor, Class III PI 3-kinase inhibitor; also inhibits autophagy 0
SF1126 Investigational Pharma inhibitor Kinase Inhibitors 0
XL147 Investigational Pharma inhibitor PI3K inhibitor,selective and reversible, Kinase Inhibitors, Phosphatidylinositol-3- kinase (PI-3K) inhibitors 0
BAY80-6946 Pharma Inhibition, Biomarker, inhibitor PI3K Inhibitors, Kinase Inhibitors 0

(4) Additional Compounds for PIK3R4 Gene - From: HMDB and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Antagonist, Gating inhibitor 58-64-0
ETP 45658
1198357-79-7
iMDK
881970-80-5
PI 103 hydrochloride
371935-79-4
genes like me logo Genes that share compounds with PIK3R4: view

Transcripts for PIK3R4 Gene

Unigene Clusters for PIK3R4 Gene

Phosphoinositide-3-kinase, regulatory subunit 4:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PIK3R4

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PIK3R4 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c ^ 13a · 13b ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^
SP1: - - -
SP2:
SP3:
SP4: - -
SP5: -
SP6: - - - - -

ExUns: 21 ^ 22 ^ 23
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:

Relevant External Links for PIK3R4 Gene

GeneLoc Exon Structure for
PIK3R4
ECgene alternative splicing isoforms for
PIK3R4

Expression for PIK3R4 Gene

mRNA expression in normal human tissues for PIK3R4 Gene

Protein differential expression in normal tissues from HIPED for PIK3R4 Gene

This gene is overexpressed in Platelet (11.2), Bone marrow mesenchymal stem cell (9.2), and Lymph node (9.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for PIK3R4 Gene



SOURCE GeneReport for Unigene cluster for PIK3R4 Gene Hs.149032

mRNA Expression by UniProt/SwissProt for PIK3R4 Gene

Q99570-PI3R4_HUMAN
Tissue specificity: Ubiquitously expressed.
genes like me logo Genes that share expression patterns with PIK3R4: view

Protein tissue co-expression partners for PIK3R4 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for PIK3R4 Gene

Orthologs for PIK3R4 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for PIK3R4 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia PIK3R4 35
  • 88.91 (n)
  • 96.61 (a)
PIK3R4 36
  • 97 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PIK3R4 35
  • 93.2 (n)
  • 97.79 (a)
PIK3R4 36
  • 98 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Pik3r4 35
  • 85.71 (n)
  • 95.88 (a)
Pik3r4 16
Pik3r4 36
  • 96 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia PIK3R4 35
  • 99.78 (n)
  • 99.93 (a)
PIK3R4 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Pik3r4 35
  • 85.78 (n)
  • 95.72 (a)
oppossum
(Monodelphis domestica)
Mammalia PIK3R4 36
  • 93 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PIK3R4 36
  • 88 (a)
OneToOne
chicken
(Gallus gallus)
Aves PIK3R4 35
  • 80.73 (n)
  • 91.81 (a)
PIK3R4 36
  • 93 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PIK3R4 36
  • 90 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia pik3r4 35
  • 76.57 (n)
  • 86.99 (a)
Str.13268 35
zebrafish
(Danio rerio)
Actinopterygii pik3r4 35
  • 72.04 (n)
  • 84.32 (a)
pik3r4 36
  • 82 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta CG9746 37
  • 35 (a)
ird1 35
  • 50.5 (n)
  • 44.86 (a)
ird1 36
  • 41 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000602 35
  • 49.51 (n)
  • 47.09 (a)
worm
(Caenorhabditis elegans)
Secernentea ZK930.1 37
  • 31 (a)
vps-15 36
  • 27 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL316C 35
  • 42.9 (n)
  • 31.7 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B11924g 35
  • 44.04 (n)
  • 31.5 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes VPS15 35
  • 44.08 (n)
  • 32.99 (a)
VPS15 36
  • 26 (a)
OneToOne
VPS15 38
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G29380 35
  • 48.51 (n)
  • 40.37 (a)
rice
(Oryza sativa)
Liliopsida Os02g0796700 35
  • 44.24 (n)
  • 32.98 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU06626 35
  • 46.67 (n)
  • 38.23 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ppk19 35
  • 49.24 (n)
  • 40.5 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 45 (a)
OneToOne
Species with no ortholog for PIK3R4:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PIK3R4 Gene

ENSEMBL:
Gene Tree for PIK3R4 (if available)
TreeFam:
Gene Tree for PIK3R4 (if available)

Paralogs for PIK3R4 Gene

No data available for Paralogs for PIK3R4 Gene

Variants for PIK3R4 Gene

Sequence variations from dbSNP and Humsavar for PIK3R4 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type MAF
rs900988 -- 130,699,703(+) ctctg(C/T)cattC intron-variant
rs900989 -- 130,700,537(+) CAAAG(A/G)GACAA intron-variant
rs1014243 -- 130,727,692(-) ATATA(A/T)GCTAA intron-variant
rs1542648 -- 130,740,883(-) ttaac(A/G)ttttg intron-variant
rs1682461 -- 130,745,798(-) GATCC(G/T)CCCGC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PIK3R4 Gene

Variant ID Type Subtype PubMed ID
esv34166 CNV Loss 18971310
nsv460855 CNV Loss 19166990
nsv877497 CNV Loss 21882294
nsv509852 CNV Loss 20534489

Variation tolerance for PIK3R4 Gene

Residual Variation Intolerance Score: 18.96% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.53; 55.68% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PIK3R4 Gene

HapMap Linkage Disequilibrium report
PIK3R4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PIK3R4 Gene

Disorders for PIK3R4 Gene

Relevant External Links for PIK3R4

Human Genome Epidemiology (HuGE) Navigator
PIK3R4
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PIK3R4
genes like me logo Genes that share disorders with PIK3R4: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PIK3R4 Gene

Publications for PIK3R4 Gene

  1. Regulation of Beclin 1 in autophagy. (PMID: 19372752) Sun Q. … Zhong Q. (Autophagy 2009) 23 67
  2. Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. (PMID: 18843052) Itakura E. … Mizushima N. (Mol. Biol. Cell 2008) 23 67
  3. Human VPS34 and p150 are Rab7 interacting partners. (PMID: 14617358) Stein M.P. … Wandinger-Ness A. (Traffic 2003) 23 67
  4. Distinct classes of phosphatidylinositol 3'-kinases are involved in signaling pathways that control macroautophagy in HT-29 cells. (PMID: 10625637) Petiot A. … Codogno P. (J. Biol. Chem. 2000) 23 67
  5. Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.PtdIns 3-kinase complex. (PMID: 8999962) Panaretou C. … Waterfield M.D. (J. Biol. Chem. 1997) 2 67

Products for PIK3R4 Gene

Sources for PIK3R4 Gene

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