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Aliases for PIK3R4 Gene

Aliases for PIK3R4 Gene

  • Phosphoinositide-3-Kinase Regulatory Subunit 4 2 3 5
  • Phosphoinositide 3-Kinase Adaptor Protein 3 4
  • PI3-Kinase Regulatory Subunit 4 3 4
  • PI3-Kinase P150 Subunit 3 4
  • EC 2.7.11.1 4 61
  • VPS15 3 4
  • Phosphoinositide-3-Kinase, Regulatory Subunit 4, P150 3
  • Phosphoinositide-3-Kinase, Regulatory Subunit 4 2
  • Phosphoinositide 3-Kinase Regulatory Subunit 4 3
  • Phosphatidylinositol 3-Kinase-Associated P150 3
  • P150 3

External Ids for PIK3R4 Gene

Previous GeneCards Identifiers for PIK3R4 Gene

  • GC03P127093
  • GC03M131114
  • GC03M131679
  • GC03M131718
  • GC03M131630
  • GC03M131880
  • GC03M130397
  • GC03M127782

Summaries for PIK3R4 Gene

GeneCards Summary for PIK3R4 Gene

PIK3R4 (Phosphoinositide-3-Kinase Regulatory Subunit 4) is a Protein Coding gene. Among its related pathways are IL-2 Pathway and Metabolism. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and binding.

UniProtKB/Swiss-Prot for PIK3R4 Gene

  • Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).

Tocris Summary for PIK3R4 Gene

  • PI 3-Kinases (phosphoinositide 3-kinases, PI 3-Ks) are a family of lipid kinases capable of phosphorylating the 3'OH of the inositol ring of phosphoinositides. They are responsible for coordinating a diverse range of cell functions including proliferation and survival.

Gene Wiki entry for PIK3R4 Gene

No data available for Entrez Gene Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PIK3R4 Gene

Genomics for PIK3R4 Gene

Regulatory Elements for PIK3R4 Gene

Enhancers for PIK3R4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH03F131501 0.4 ENCODE 22.2 -755.8 -755761 2.4 HDGF PKNOX1 CREB3L1 MLX WRNIP1 ZFP64 ARID4B SIN3A DMAP1 ZNF2 PIK3R4 MRPL3 ENSG00000261167 LOC105374114
GH03F130657 0.9 Ensembl ENCODE 17.6 +86.3 86270 5.7 HDAC1 PKNOX1 FEZF1 ZNF2 GATA2 ZNF366 SCRT2 FOS ZNF263 ZNF680 PIK3R4 GC03P130677 GC03P130602
GH03F130726 0.4 Ensembl 17 +19.7 19651 1.2 ZNF341 CEBPB NBN PRDM10 ZBTB33 ZBTB26 CHAMP1 PIK3R4 GC03P130677
GH03F131020 0.8 Ensembl ENCODE 16 -273.6 -273649 0.6 ZNF202 RNF2 MAX EBF1 ZNF664 HMBOX1 ZBTB33 MYC SPI1 NEK11 PIK3R4 ASTE1 ENSG00000250592
GH03F130745 0.2 ENCODE 15.3 -0.2 -229 3.3 HDGF PKNOX1 ATF1 ARID4B SIN3A DMAP1 ZNF48 BRCA1 ETS1 YY1 PIK3R4 RN7SKP212
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around PIK3R4 on UCSC Golden Path with GeneCards custom track

Genomic Location for PIK3R4 Gene

Chromosome:
3
Start:
130,678,934 bp from pter
End:
130,746,852 bp from pter
Size:
67,919 bases
Orientation:
Minus strand

Genomic View for PIK3R4 Gene

Genes around PIK3R4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PIK3R4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PIK3R4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PIK3R4 Gene

Proteins for PIK3R4 Gene

  • Protein details for PIK3R4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99570-PI3R4_HUMAN
    Recommended name:
    Phosphoinositide 3-kinase regulatory subunit 4
    Protein Accession:
    Q99570
    Secondary Accessions:
    • Q2TBF4

    Protein attributes for PIK3R4 Gene

    Size:
    1358 amino acids
    Molecular mass:
    153103 Da
    Cofactor:
    Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
    Quaternary structure:
    • Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG (PubMed:8999962, PubMed:19270696, PubMed:23878393, PubMed:25490155). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (PubMed:25490155). Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1, AMBRA1 and NRBF2 (PubMed:19270696, PubMed:20643123, PubMed:24785657). PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3C3/VPS34 (PubMed:14617358). Interacts with NRBF2 (PubMed:24785657).

neXtProt entry for PIK3R4 Gene

Post-translational modifications for PIK3R4 Gene

Other Protein References for PIK3R4 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PIK3R4 (PIK3R4)
  • Santa Cruz Biotechnology (SCBT) Antibodies for PIK3R4

Domains & Families for PIK3R4 Gene

Suggested Antigen Peptide Sequences for PIK3R4 Gene

Graphical View of Domain Structure for InterPro Entry

Q99570

UniProtKB/Swiss-Prot:

PI3R4_HUMAN :
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
  • Contains 3 HEAT repeats.
Domain:
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Similarity:
  • Contains 3 HEAT repeats.
  • Contains 7 WD repeats.
genes like me logo Genes that share domains with PIK3R4: view

Function for PIK3R4 Gene

Molecular function for PIK3R4 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot Function:
Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).

Enzyme Numbers (IUBMB) for PIK3R4 Gene

Gene Ontology (GO) - Molecular Function for PIK3R4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity NAS 8999962
GO:0004674 protein serine/threonine kinase activity IEA,IBA --
GO:0005515 protein binding IPI 14617358
GO:0005524 ATP binding IEA --
GO:0016301 kinase activity IEA --
genes like me logo Genes that share ontologies with PIK3R4: view
genes like me logo Genes that share phenotypes with PIK3R4: view

Animal Models for PIK3R4 Gene

MGI Knock Outs for PIK3R4:

Animal Model Products

  • Taconic Biosciences Mouse Models for PIK3R4

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PIK3R4 Gene

Localization for PIK3R4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PIK3R4 Gene

Late endosome. Cytoplasmic vesicle, autophagosome. Membrane; Lipid-anchor. Note=As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (By similarity). {ECO:0000250 UniProtKB:Q8VD65, ECO:0000305}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PIK3R4 Gene COMPARTMENTS Subcellular localization image for PIK3R4 gene
Compartment Confidence
cytosol 5
endosome 5
nucleus 4
vacuole 4
cytoskeleton 2
lysosome 2
golgi apparatus 1
mitochondrion 1

Gene Ontology (GO) - Cellular Components for PIK3R4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005643 nuclear pore IBA --
GO:0005768 endosome IEA --
GO:0005770 late endosome IEA,IDA 14617358
GO:0005776 autophagosome IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with PIK3R4: view

Pathways & Interactions for PIK3R4 Gene

genes like me logo Genes that share pathways with PIK3R4: view

SIGNOR curated interactions for PIK3R4 Gene

Activates:

Gene Ontology (GO) - Biological Process for PIK3R4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation NAS 8999962
GO:0006623 protein targeting to vacuole IBA --
GO:0006661 phosphatidylinositol biosynthetic process TAS --
GO:0016236 macroautophagy TAS --
GO:0016310 phosphorylation IEA --
genes like me logo Genes that share ontologies with PIK3R4: view

Drugs & Compounds for PIK3R4 Gene

(27) Drugs for PIK3R4 Gene - From: DGIdb, HMDB, and Tocris

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
Manganese Approved Nutra 34
3-Methyladenine Experimental Pharma Class III PI3K inhibitor, Class III PI 3-kinase inhibitor; also inhibits autophagy 0
SF1126 Investigational Pharma inhibitor Kinase Inhibitors 0
XL147 Investigational Pharma inhibitor PI3K inhibitor,selective and reversible, Kinase Inhibitors, Phosphatidylinositol-3- kinase (PI-3K) inhibitors 0

(2) Additional Compounds for PIK3R4 Gene - From: Tocris and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
LY 294002 hydrochloride
934389-88-5

(5) Tocris Compounds for PIK3R4 Gene

Compound Action Cas Number
3-Methyladenine Class III PI 3-kinase inhibitor; also inhibits autophagy 5142-23-4
740 Y-P Cell-permeable PI 3-kinase activator 1236188-16-1
LY 294002 hydrochloride Prototypical PI 3-kinase inhibitor; also inhibits other kinases 934389-88-5
PI 103 hydrochloride Inhibitor of PI 3-kinase, mTOR and DNA-PK 371935-79-4
PI 828 PI 3-kinase inhibitor, more potent than LY 294002 (Cat. No. 1130) 942289-87-4
genes like me logo Genes that share compounds with PIK3R4: view

Transcripts for PIK3R4 Gene

Unigene Clusters for PIK3R4 Gene

Phosphoinositide-3-kinase, regulatory subunit 4:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PIK3R4 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c ^ 13a · 13b ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^
SP1: - - -
SP2:
SP3:
SP4: - -
SP5: -
SP6: - - - - -

ExUns: 21 ^ 22 ^ 23
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:

Relevant External Links for PIK3R4 Gene

GeneLoc Exon Structure for
PIK3R4
ECgene alternative splicing isoforms for
PIK3R4

Expression for PIK3R4 Gene

mRNA expression in normal human tissues for PIK3R4 Gene

Protein differential expression in normal tissues from HIPED for PIK3R4 Gene

This gene is overexpressed in Platelet (11.2), Bone marrow mesenchymal stem cell (9.2), and Lymph node (9.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PIK3R4 Gene



Protein tissue co-expression partners for PIK3R4 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of PIK3R4 Gene:

PIK3R4

SOURCE GeneReport for Unigene cluster for PIK3R4 Gene:

Hs.149032

mRNA Expression by UniProt/SwissProt for PIK3R4 Gene:

Q99570-PI3R4_HUMAN
Tissue specificity: Ubiquitously expressed.
genes like me logo Genes that share expression patterns with PIK3R4: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for PIK3R4 Gene

Orthologs for PIK3R4 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for PIK3R4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PIK3R4 34 35
  • 99.78 (n)
dog
(Canis familiaris)
Mammalia PIK3R4 34 35
  • 93.2 (n)
oppossum
(Monodelphis domestica)
Mammalia PIK3R4 35
  • 93 (a)
OneToOne
cow
(Bos Taurus)
Mammalia PIK3R4 34 35
  • 88.91 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia PIK3R4 35
  • 88 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Pik3r4 34
  • 85.78 (n)
mouse
(Mus musculus)
Mammalia Pik3r4 34 16 35
  • 85.71 (n)
chicken
(Gallus gallus)
Aves PIK3R4 34 35
  • 80.73 (n)
lizard
(Anolis carolinensis)
Reptilia PIK3R4 35
  • 90 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia pik3r4 34
  • 76.57 (n)
Str.13268 34
zebrafish
(Danio rerio)
Actinopterygii pik3r4 34 35
  • 72.04 (n)
fruit fly
(Drosophila melanogaster)
Insecta ird1 34 35
  • 50.5 (n)
CG9746 36
  • 35 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000602 34
  • 49.51 (n)
worm
(Caenorhabditis elegans)
Secernentea ZK930.1 36
  • 31 (a)
vps-15 35
  • 27 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes VPS15 34 35 37
  • 44.08 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B11924g 34
  • 44.04 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL316C 34
  • 42.9 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G29380 34
  • 48.51 (n)
rice
(Oryza sativa)
Liliopsida Os02g0796700 34
  • 44.24 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ppk19 34
  • 49.24 (n)
bread mold
(Neurospora crassa)
Ascomycetes NCU06626 34
  • 46.67 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 45 (a)
OneToOne
Species where no ortholog for PIK3R4 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PIK3R4 Gene

ENSEMBL:
Gene Tree for PIK3R4 (if available)
TreeFam:
Gene Tree for PIK3R4 (if available)

Paralogs for PIK3R4 Gene

No data available for Paralogs for PIK3R4 Gene

Variants for PIK3R4 Gene

Sequence variations from dbSNP and Humsavar for PIK3R4 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type
rs181132426 A breast cancer sample 130,705,686(+) TAATT(C/T)GAATC reference, missense
rs1014243 -- 130,727,692(-) ATATA(A/T)GCTAA intron-variant
rs10433410 -- 130,722,440(+) AAAAA(A/T)CTATT intron-variant
rs10433411 -- 130,723,307(+) GGAAC(C/G)CCACA intron-variant
rs10512786 -- 130,683,704(+) CATTA(A/G)TTGTG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PIK3R4 Gene

Variant ID Type Subtype PubMed ID
esv34166 CNV loss 18971310
nsv1005160 CNV gain 25217958
nsv460855 CNV loss 19166990
nsv509852 OTHER sequence alteration 20534489

Variation tolerance for PIK3R4 Gene

Residual Variation Intolerance Score: 19% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.53; 55.68% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PIK3R4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PIK3R4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PIK3R4 Gene

Disorders for PIK3R4 Gene

Relevant External Links for PIK3R4

Human Genome Epidemiology (HuGE) Navigator
PIK3R4
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PIK3R4

No disorders were found for PIK3R4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PIK3R4 Gene

Publications for PIK3R4 Gene

  1. Human VPS34 and p150 are Rab7 interacting partners. (PMID: 14617358) Stein M.P. … Wandinger-Ness A. (Traffic 2003) 3 4 22 64
  2. Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.PtdIns 3-kinase complex. (PMID: 8999962) Panaretou C. … Waterfield M.D. (J. Biol. Chem. 1997) 2 3 4 64
  3. NRBF2 regulates macroautophagy as a component of Vps34 Complex I. (PMID: 24785657) Cao Y. … Backer J.M. (Biochem. J. 2014) 3 4 64
  4. Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy. (PMID: 23878393) Fogel A.I. … Youle R.J. (Mol. Cell. Biol. 2013) 3 4 64
  5. A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic. (PMID: 20643123) Thoresen S.B. … Stenmark H. (Exp. Cell Res. 2010) 3 4 64

Products for PIK3R4 Gene

Sources for PIK3R4 Gene

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