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Aliases for PIAS4 Gene

Aliases for PIAS4 Gene

  • Protein Inhibitor Of Activated STAT 4 2 3 5
  • Protein Inhibitor Of Activated STAT Protein Gamma 3 4
  • Protein Inhibitor Of Activated STAT Protein 4 3 4
  • Zinc Finger, MIZ-Type Containing 6 2 3
  • PIAS-Gamma 3 4
  • PIASY 3 4
  • Piasg 3 4
  • Protein Inhibitor Of Activated STAT Protein PIASy 3
  • E3 SUMO-Protein Ligase PIAS4 3
  • EC 6.3.2.- 4
  • ZMIZ6 3

External Ids for PIAS4 Gene

Previous GeneCards Identifiers for PIAS4 Gene

  • GC19P003959
  • GC19P003960
  • GC19P003773

Summaries for PIAS4 Gene

GeneCards Summary for PIAS4 Gene

PIAS4 (Protein Inhibitor Of Activated STAT 4) is a Protein Coding gene. Among its related pathways are Regulation of nuclear SMAD2/3 signaling and DNA Double Strand Break Response. GO annotations related to this gene include ligase activity and SUMO transferase activity. An important paralog of this gene is PIAS1.

UniProtKB/Swiss-Prot for PIAS4 Gene

  • Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

Gene Wiki entry for PIAS4 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PIAS4 Gene

Genomics for PIAS4 Gene

Regulatory Elements for PIAS4 Gene

Enhancers for PIAS4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH19F004006 1.3 FANTOM5 ENCODE 5.7 +1.5 1482 4.7 CREB3L1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 CBX5 ZNF143 ZNF207 PIAS4 ATCAY ZFR2 MATK GC19M003988
GH19F003981 0.2 ENCODE 4.9 -23.0 -23012 8.2 CREB3L1 FEZF1 DMAP1 YY1 SLC30A9 ZNF143 ZNF263 SP3 NFYC MEF2D CACTIN ENSG00000267030 ENSG00000267769 ENSG00000267255 ENSG00000266959 LOC105372244 LOC100287614 SH3GL1 CACTIN-AS1 APBA3
GH19F003525 0.9 FANTOM5 Ensembl ENCODE 1.1 -469.9 -469888 23.7 HDGF PKNOX1 ARID4B SIN3A GLI4 ZNF2 ZNF121 ZNF143 KLF13 ZNF263 MFSD12 C19orf71 FZR1 PIP5K1C S1PR4 GNA15 PIAS4 GC19P003535 PIR52859
GH19F004011 0.6 ENCODE 0.8 +4.9 4894 1.0 OSR2 ZFHX2 ZNF680 EGR2 YY1 PIAS4 GC19M003988
GH19F004014 1.2 FANTOM5 Ensembl ENCODE 0.4 +7.9 7923 2.4 ZNF263 HDGF PKNOX1 SP2 NR3C1 PIAS4 GC19M003988
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around PIAS4 on UCSC Golden Path with GeneCards custom track

Promoters for PIAS4 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000042543 202 2401 CREB3L1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 CBX5 ZNF143 ZNF207

Genomic Location for PIAS4 Gene

Chromosome:
19
Start:
4,007,598 bp from pter
End:
4,039,386 bp from pter
Size:
31,789 bases
Orientation:
Plus strand

Genomic View for PIAS4 Gene

Genes around PIAS4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PIAS4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PIAS4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PIAS4 Gene

Proteins for PIAS4 Gene

  • Protein details for PIAS4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8N2W9-PIAS4_HUMAN
    Recommended name:
    E3 SUMO-protein ligase PIAS4
    Protein Accession:
    Q8N2W9
    Secondary Accessions:
    • O75926
    • Q96G19
    • Q9UN16

    Protein attributes for PIAS4 Gene

    Size:
    510 amino acids
    Molecular mass:
    56504 Da
    Quaternary structure:
    • Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with MTA1.
    SequenceCaution:
    • Sequence=AAD45155.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for PIAS4 Gene

Post-translational modifications for PIAS4 Gene

  • Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Ubiquitination at Lys 114, Lys 231, and Lys 504
  • Modification sites at PhosphoSitePlus

Other Protein References for PIAS4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PIAS4 (PIAS4)

No data available for DME Specific Peptides for PIAS4 Gene

Domains & Families for PIAS4 Gene

Gene Families for PIAS4 Gene

Protein Domains for PIAS4 Gene

Suggested Antigen Peptide Sequences for PIAS4 Gene

Graphical View of Domain Structure for InterPro Entry

Q8N2W9

UniProtKB/Swiss-Prot:

PIAS4_HUMAN :
  • The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Belongs to the PIAS family.
  • Contains 1 SP-RING-type zinc finger.
Domain:
  • The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Contains 1 PINIT domain.
  • Contains 1 SAP domain.
Family:
  • Belongs to the PIAS family.
Similarity:
  • Contains 1 SP-RING-type zinc finger.
genes like me logo Genes that share domains with PIAS4: view

Function for PIAS4 Gene

Molecular function for PIAS4 Gene

UniProtKB/Swiss-Prot Function:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

Enzyme Numbers (IUBMB) for PIAS4 Gene

Gene Ontology (GO) - Molecular Function for PIAS4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003714 transcription corepressor activity IEA --
GO:0005515 protein binding IPI 11388671
GO:0008022 protein C-terminus binding IEA --
GO:0008270 zinc ion binding NAS 9724754
genes like me logo Genes that share ontologies with PIAS4: view
genes like me logo Genes that share phenotypes with PIAS4: view

Animal Models for PIAS4 Gene

MGI Knock Outs for PIAS4:

Animal Model Products

  • Taconic Biosciences Mouse Models for PIAS4

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for PIAS4

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PIAS4 Gene

Localization for PIAS4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PIAS4 Gene

Nucleus, PML body. Note=Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PIAS4 Gene COMPARTMENTS Subcellular localization image for PIAS4 gene
Compartment Confidence
nucleus 5
cytosol 2
extracellular 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for PIAS4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus NAS 9724754
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IDA 16816390
GO:0016363 nuclear matrix IEA --
GO:0016605 PML body IEA --
genes like me logo Genes that share ontologies with PIAS4: view

Pathways & Interactions for PIAS4 Gene

genes like me logo Genes that share pathways with PIAS4: view

UniProtKB/Swiss-Prot Q8N2W9-PIAS4_HUMAN

  • Pathway: Protein modification; protein sumoylation.

SIGNOR curated interactions for PIAS4 Gene

Activates:
Inactivates:

Gene Ontology (GO) - Biological Process for PIAS4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA --
GO:0006303 double-strand break repair via nonhomologous end joining TAS --
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway IEA --
genes like me logo Genes that share ontologies with PIAS4: view

Drugs & Compounds for PIAS4 Gene

(2) Drugs for PIAS4 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with PIAS4: view

Transcripts for PIAS4 Gene

Unigene Clusters for PIAS4 Gene

Protein inhibitor of activated STAT, 4:
Representative Sequences:

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for PIAS4

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PIAS4 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14
SP1: - - -
SP2:
SP3: - - - -
SP4: - -
SP5: -

Relevant External Links for PIAS4 Gene

GeneLoc Exon Structure for
PIAS4
ECgene alternative splicing isoforms for
PIAS4

Expression for PIAS4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PIAS4 Gene

Protein differential expression in normal tissues from HIPED for PIAS4 Gene

This gene is overexpressed in Fetal Brain (18.4), Urinary Bladder (15.1), Retina (8.6), and Liver (8.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PIAS4 Gene



Protein tissue co-expression partners for PIAS4 Gene

NURSA nuclear receptor signaling pathways regulating expression of PIAS4 Gene:

PIAS4

SOURCE GeneReport for Unigene cluster for PIAS4 Gene:

Hs.105779

mRNA Expression by UniProt/SwissProt for PIAS4 Gene:

Q8N2W9-PIAS4_HUMAN
Tissue specificity: Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
genes like me logo Genes that share expression patterns with PIAS4: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for PIAS4 Gene

Orthologs for PIAS4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PIAS4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PIAS4 35
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia PIAS4 35
  • 91 (a)
OneToOne
cow
(Bos Taurus)
Mammalia PIAS4 34 35
  • 89.28 (n)
dog
(Canis familiaris)
Mammalia PIAS4 34 35
  • 88.69 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 88 (a)
OneToMany
-- 35
  • 84 (a)
OneToMany
mouse
(Mus musculus)
Mammalia Pias4 34 16 35
  • 83.53 (n)
rat
(Rattus norvegicus)
Mammalia Pias4 34
  • 83.27 (n)
chicken
(Gallus gallus)
Aves PIAS4 34 35
  • 82.33 (n)
lizard
(Anolis carolinensis)
Reptilia PIAS4 35
  • 73 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia pias4 34
  • 69.78 (n)
Str.11012 34
African clawed frog
(Xenopus laevis)
Amphibia LOC398703 34
zebrafish
(Danio rerio)
Actinopterygii pias4a 34 35
  • 63.15 (n)
pias4b 35
  • 41 (a)
OneToMany
wufi20e09 34
fruit fly
(Drosophila melanogaster)
Insecta Su(var)2-10 35
  • 31 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea gei-17 35
  • 17 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NFI1 35 37
  • 12 (a)
ManyToMany
SIZ1 35
  • 9 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 24 (a)
OneToMany
Species where no ortholog for PIAS4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PIAS4 Gene

ENSEMBL:
Gene Tree for PIAS4 (if available)
TreeFam:
Gene Tree for PIAS4 (if available)

Paralogs for PIAS4 Gene

Paralogs for PIAS4 Gene

(5) SIMAP similar genes for PIAS4 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with PIAS4: view

Variants for PIAS4 Gene

Sequence variations from dbSNP and Humsavar for PIAS4 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
rs10405522 -- 4,039,058(+) GCCCC(C/G)CAGGA utr-variant-3-prime
rs10405525 -- 4,039,061(+) CCGCA(G/T)GAGAC utr-variant-3-prime
rs10407319 -- 4,039,846(+) CAAAT(A/G)TTTTG downstream-variant-500B
rs10408925 -- 4,039,839(+) CGATA(C/T)ACAAA downstream-variant-500B
rs10412178 -- 4,027,726(+) gggta(A/T)ttttt intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PIAS4 Gene

Variant ID Type Subtype PubMed ID
dgv495e201 CNV deletion 23290073
esv2658425 CNV deletion 23128226
esv2662737 CNV deletion 23128226
esv2670138 CNV deletion 23128226
esv2671087 CNV deletion 23128226
esv2717979 CNV deletion 23290073
esv2717980 CNV deletion 23290073
esv2717981 CNV deletion 23290073
esv2717982 CNV deletion 23290073
esv2717983 CNV deletion 23290073
esv2717986 CNV deletion 23290073
esv2717988 CNV deletion 23290073
esv3555892 CNV deletion 23714750
esv3643486 CNV gain 21293372
esv9150 CNV loss 19470904
nsv1120352 CNV tandem duplication 24896259
nsv1146574 CNV insertion 26484159
nsv470115 CNV loss 18288195
nsv476927 CNV novel sequence insertion 20440878
nsv480690 CNV novel sequence insertion 20440878
nsv519665 CNV loss 19592680
nsv578435 CNV loss 21841781
nsv828401 CNV gain 20364138
nsv828402 CNV gain 20364138
nsv953949 CNV deletion 24416366

Variation tolerance for PIAS4 Gene

Residual Variation Intolerance Score: 13.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.25; 25.01% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PIAS4 Gene

Human Gene Mutation Database (HGMD)
PIAS4
SNPedia medical, phenotypic, and genealogical associations of SNPs for
PIAS4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PIAS4 Gene

Disorders for PIAS4 Gene

Relevant External Links for PIAS4

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PIAS4

No disorders were found for PIAS4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PIAS4 Gene

Publications for PIAS4 Gene

  1. SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy- dependent activation of Tcf-4. (PMID: 15831457) Ihara M. … Kikuchi A. (Mol. Cell. Biol. 2005) 3 4 22 64
  2. Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner. (PMID: 12631292) Dahle O. … Gabrielsen O.S. (Eur. J. Biochem. 2003) 3 4 22 64
  3. Sumoylation is involved in beta-catenin-dependent activation of Tcf- 4. (PMID: 12727872) Yamamoto H. … Kikuchi A. (EMBO J. 2003) 3 4 22 64
  4. Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy. (PMID: 12750312) Chun T.-H. … Nakao K. (Circ. Res. 2003) 3 4 22 64
  5. A transcriptional corepressor of Stat1 with an essential LXXLL signature motif. (PMID: 11248056) Liu B. … Shuai K. (Proc. Natl. Acad. Sci. U.S.A. 2001) 3 4 22 64

Products for PIAS4 Gene

Sources for PIAS4 Gene

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