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PIAS3 Gene(Protein Coding)

Protein Inhibitor Of Activated STAT 3

GCID:
GC01M145848
GIFtS:
60
Genes Participants
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Aliases for PIAS3 Gene

Aliases for PIAS3 Gene

  • Protein Inhibitor Of Activated STAT 3 2 3 5
  • Protein Inhibitor Of Activated STAT Protein 3 3 4
  • Protein Inhibitor Of Activated STAT, 3 3
  • Zinc Finger, MIZ-Type Containing 5 3
  • MIZ-Type Containing 5 2
  • Zinc Finger 2
  • EC 6.3.2.- 4
  • ZMIZ5 3

External Ids for PIAS3 Gene

Previous GeneCards Identifiers for PIAS3 Gene

  • GC01P144096
  • GC01P142163
  • GC01P143302
  • GC01P143324
  • GC01P143065
  • GC01P144287
  • GC01P145575
  • GC01P119540

Summaries for PIAS3 Gene

Entrez Gene Summary for PIAS3 Gene

  • This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

GeneCards Summary for PIAS3 Gene

PIAS3 (Protein Inhibitor Of Activated STAT 3) is a Protein Coding gene. Among its related pathways are Transcription-Coupled Nucleotide Excision Repair (TC-NER) and Androgen receptor signaling pathway. GO annotations related to this gene include nucleic acid binding and ligase activity. An important paralog of this gene is ZMIZ1.

UniProtKB/Swiss-Prot for PIAS3 Gene

  • Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).

Gene Wiki entry for PIAS3 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PIAS3 Gene

Genomics for PIAS3 Gene

Products:

  1. Regulatory Element

Regulatory Elements for PIAS3 Gene

Enhancers for PIAS3 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around PIAS3 on UCSC Golden Path with GeneCards custom track

Promoters for PIAS3 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around PIAS3 on UCSC Golden Path with GeneCards custom track

Transcription factor binding sites by QIAGEN in the PIAS3 gene promoter:

Genomic Location for PIAS3 Gene

Chromosome:
1
Start:
145,848,522 bp from pter
End:
145,859,836 bp from pter
Size:
11,315 bases
Orientation:
Minus strand

Genomic View for PIAS3 Gene

Genes around PIAS3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PIAS3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PIAS3 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for PIAS3 Gene

Proteins for PIAS3 Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for PIAS3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y6X2-PIAS3_HUMAN
    Recommended name:
    E3 SUMO-protein ligase PIAS3
    Protein Accession:
    Q9Y6X2
    Secondary Accessions:
    • Q9UFI3

    Protein attributes for PIAS3 Gene

    Size:
    628 amino acids
    Molecular mass:
    68017 Da
    Quaternary structure:
    • Monomer (By similarity). Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity (By similarity). Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. Interacts with MTA1. Interacts with CCAR2 (via N-terminus).
    SequenceCaution:
    • Sequence=BAA78533.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for PIAS3 Gene

neXtProt entry for PIAS3 Gene

Proteomics data for PIAS3 Gene at MOPED

Protein Expression for PIAS3 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for PIAS3 Gene

Other Protein References for PIAS3 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PIAS3 (PIAS3)
  • Abcam antibodies for PIAS3

No data available for DME Specific Peptides for PIAS3 Gene

Domains & Families for PIAS3 Gene

Gene Families for PIAS3 Gene

HGNC:

Protein Domains for PIAS3 Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for PIAS3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9Y6X2

UniProtKB/Swiss-Prot:

PIAS3_HUMAN :
  • The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
  • Belongs to the PIAS family.
  • Contains 1 SP-RING-type zinc finger.
Domain:
  • The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
  • The LXXLL motif is a transcriptional coregulator signature.
  • Contains 1 PINIT domain.
  • Contains 1 SAP domain.
Family:
  • Belongs to the PIAS family.
Similarity:
  • Contains 1 SP-RING-type zinc finger.
genes like me logo Genes that share domains with PIAS3: view

Function for PIAS3 Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line
  7. Flow Cytometry

Molecular function for PIAS3 Gene

UniProtKB/Swiss-Prot Function:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
UniProtKB/Swiss-Prot Induction:
By dihydrotestosterone (DHT) in prostate cancer cells.

Enzyme Numbers (IUBMB) for PIAS3 Gene

Gene Ontology (GO) - Molecular Function for PIAS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 11060035
GO:0016874 ligase activity IEA --
GO:0047485 protein N-terminus binding IEA --
genes like me logo Genes that share ontologies with PIAS3: view

Phenotypes for PIAS3 Gene

GenomeRNAi human phenotypes for PIAS3:
genes like me logo Genes that share phenotypes with PIAS3: view

Animal Model Products

miRNA for PIAS3 Gene

miRTarBase miRNAs that target PIAS3

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for PIAS3 Gene

Localization for PIAS3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PIAS3 Gene

Cytoplasm. Nucleus. Nucleus speckle. Note=Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules. {ECO:0000250 UniProtKB:O54714}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PIAS3 Gene COMPARTMENTS Subcellular localization image for PIAS3 gene
Compartment Confidence
nucleus 3
cytosol 2

Gene Ontology (GO) - Cellular Components for PIAS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0030425 dendrite IEA --
genes like me logo Genes that share ontologies with PIAS3: view

Pathways & Interactions for PIAS3 Gene

genes like me logo Genes that share pathways with PIAS3: view

UniProtKB/Swiss-Prot Q9Y6X2-PIAS3_HUMAN

  • Pathway: Protein modification; protein sumoylation.

SIGNOR curated interactions for PIAS3 Gene

Activates:
Inactivates:

Gene Ontology (GO) - Biological Process for PIAS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0010628 positive regulation of gene expression IEA --
GO:0016925 protein sumoylation ISS,TAS --
GO:0033234 negative regulation of protein sumoylation IDA 24651376
genes like me logo Genes that share ontologies with PIAS3: view

Drugs & Compounds for PIAS3 Gene

Products:

  1. Drug
No Compound Related Data Available

Transcripts for PIAS3 Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone
  5. Flow Cytometry

mRNA/cDNA for PIAS3 Gene

(1) REFSEQ mRNAs :
(9) Additional mRNA sequences :
(208) Selected AceView cDNA sequences:
(9) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for PIAS3 Gene

Protein inhibitor of activated STAT, 3:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PIAS3 Gene

ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c · 5d ^ 6a · 6b ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b · 13c ^ 14 ^
SP1: - - - - -
SP2: - - -
SP3: -
SP4: - - - - - - - -
SP5: - - - - - -
SP6:
SP7:
SP8: - - - -
SP9:
SP10:
SP11: - - -
SP12: - -
SP13: -

ExUns: 15a · 15b ^ 16a · 16b · 16c ^ 17a · 17b
SP1: - - -
SP2:
SP3: - - -
SP4:
SP5:
SP6: -
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:

Relevant External Links for PIAS3 Gene

GeneLoc Exon Structure for
PIAS3
ECgene alternative splicing isoforms for
PIAS3

Expression for PIAS3 Gene

Products:

  1. Primer

mRNA expression in normal human tissues for PIAS3 Gene

mRNA expression in normal human tissues for PIAS3 Gene
mRNA expression by BioGPS for PIAS3 GenemRNA expression by GTEx for PIAS3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for PIAS3 Gene

This gene is overexpressed in Serum (55.8) and Heart (13.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for PIAS3 Gene



SOURCE GeneReport for Unigene cluster for PIAS3 Gene Hs.435761

mRNA Expression by UniProt/SwissProt for PIAS3 Gene

Q9Y6X2-PIAS3_HUMAN
Tissue specificity: Widely expressed.
genes like me logo Genes that share expression patterns with PIAS3: view

Protein tissue co-expression partners for PIAS3 Gene

- Elite partner

Primer Products

No data available for mRNA differential expression in normal tissues for PIAS3 Gene

Orthologs for PIAS3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PIAS3 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
 Mammalia PIAS3 35
  • 93.31 (n)
  • 98.09 (a)
PIAS3 36
  • 98 (a)
 OneToOne
dog
(Canis familiaris)
 Mammalia PIAS3 35
  • 92.78 (n)
  • 97.29 (a)
PIAS3 36
  • 97 (a)
 OneToOne
mouse
(Mus musculus)
 Mammalia Pias3 35
  • 90.39 (n)
  • 96.97 (a)
Pias3 16
Pias3 36
  • 97 (a)
 OneToOne
chimpanzee
(Pan troglodytes)
 Mammalia PIAS3 35
  • 95.59 (n)
  • 94.9 (a)
PIAS3 36
  • 100 (a)
 OneToOne
rat
(Rattus norvegicus)
 Mammalia Pias3 35
  • 90.23 (n)
  • 96.66 (a)
oppossum
(Monodelphis domestica)
 Mammalia PIAS3 36
  • 84 (a)
 OneToOne
platypus
(Ornithorhynchus anatinus)
 Mammalia -- 36
  • 15 (a)
 OneToMany
-- 36
  • 38 (a)
 OneToMany
-- 36
  • 27 (a)
 OneToMany
-- 36
  • 20 (a)
 OneToMany
-- 36
  • 22 (a)
 OneToMany
-- 36
  • 32 (a)
 OneToMany
-- 36
  • 23 (a)
 OneToMany
-- 36
  • 43 (a)
 OneToMany
chicken
(Gallus gallus)
 Aves PIAS3 36
  • 59 (a)
 OneToOne
lizard
(Anolis carolinensis)
 Reptilia PIAS3 36
  • 66 (a)
 OneToOne
tropical clawed frog
(Silurana tropicalis)
 Amphibia pias3 35
  • 69.57 (n)
  • 74.71 (a)
fruit fly
(Drosophila melanogaster)
 Insecta Su(var)2-10 37
  • 44 (a)
Su(var)2-10 36
  • 34 (a)
 OneToMany
worm
(Caenorhabditis elegans)
 Secernentea gei-17 37
  • 36 (a)
W10D5.3a 37
  • 35 (a)
W10D5.3d 37
  • 35 (a)
gei-17 36
  • 20 (a)
 OneToMany
baker's yeast
(Saccharomyces cerevisiae)
 Saccharomycetes NFI1 36
  • 14 (a)
 ManyToMany
SIZ1 36
  • 11 (a)
 ManyToMany
sea squirt
(Ciona intestinalis)
 Ascidiacea Cin.2754 35
sea squirt
(Ciona savignyi)
 Ascidiacea -- 36
  • 30 (a)
 OneToMany
Species with no ortholog for PIAS3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for PIAS3 Gene

ENSEMBL:
Gene Tree for PIAS3 (if available)
TreeFam:
Gene Tree for PIAS3 (if available)

Paralogs for PIAS3 Gene

Paralogs for PIAS3 Gene

(5) SIMAP similar genes for PIAS3 Gene using alignment to 6 proteins:

genes like me logo Genes that share paralogs with PIAS3: view

Variants for PIAS3 Gene

Sequence variations from dbSNP and Humsavar for PIAS3 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
VAR_050535 -
rs4128488 -- 145,849,751(+) AATCC(C/T)GACTT intron-variant
rs6672707 -- 145,859,829(-) TAGGC(C/G)GGGCG upstream-variant-2KB
rs7549713 -- 145,860,835(-) AGCAC(C/T)TAATG upstream-variant-2KB
rs9726019 -- 145,849,934(-) CGCCC(A/C)CAAAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PIAS3 Gene

Variant ID Type Subtype PubMed ID
nsv7181 OTHER Inversion 18451855
dgv15n16 CNV Deletion 17901297
dgv46e201 CNV Deletion 23290073
esv2422428 CNV Duplication 17116639
dgv347n71 CNV Gain 21882294
nsv831370 CNV Gain 17160897
nsv831381 CNV Gain 17160897
nsv872232 CNV Gain 21882294

Variation tolerance for PIAS3 Gene

Residual Variation Intolerance Score: 22.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.03; 60.44% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for PIAS3 Gene

Disorders for PIAS3 Gene

Relevant External Links for PIAS3

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PIAS3

No disorders were found for PIAS3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PIAS3 Gene

Publications for PIAS3 Gene

  1. Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3). (PMID: 10319586) Ueki N. … Muramatsu M.-A. (J. Hum. Genet. 1999) 2 3 4 67
  2. The association and nuclear translocation of the PIAS3-STAT3 complex is ligand and time dependent. (PMID: 19903771) Dabir S. … Dowlati A. (Mol. Cancer Res. 2009) 3 23
  3. PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element. (PMID: 17565989) Iwasaki K. … Tsuji Y. (J. Biol. Chem. 2007) 3 23
  4. Identifying a common molecular mechanism for inhibition of MITF and STAT3 by PIAS3. (PMID: 16368885) Levy C. … Razin E. (Blood 2006) 3 23
  5. Down-regulation of SHP1 and up-regulation of negative regulators of JAK/STAT signaling in HTLV-1 transformed cell lines and freshly transformed human peripheral blood CD4+ T-cells. (PMID: 14630083) Cheng J. … Marasco W.A. (Leuk. Res. 2004) 3 23

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Sources for PIAS3 Gene

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