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PIAS1 Gene

protein-coding   GIFtS: 66
GCID: GC15P068346

Protein Inhibitor Of Activated STAT, 1

(Previous name: DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1)
(Previous symbol: DDXBP1)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Protein Inhibitor Of Activated STAT, 11 2     Zinc Finger1
DDXBP11 2 3 5     GU/RH-II2
GBP2 3 5     ZMIZ32
DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 11 2     AR Interacting Protein2
Gu-Binding Protein2 3     E3 SUMO-Protein Ligase PIAS12
Protein Inhibitor Of Activated STAT Protein 12 3     Protein Inhibitor Of Activated STAT-12
DEAD/H Box-Binding Protein 12 3     Zinc Finger, MIZ-Type Containing 32
RNA Helicase II-Binding Protein2 3     EC 6.3.2.-3
MIZ-Type Containing 31     

External Ids:    HGNC: 27521   Entrez Gene: 85542   Ensembl: ENSG000000338007   OMIM: 6035665   UniProtKB: O759253   

Export aliases for PIAS1 gene to outside databases

Previous GC identifers: GC15P064182 GC15P061445 GC15P065925 GC15P066062 GC15P066133 GC15P045180


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for PIAS1 Gene:
This gene encodes a member of the mammalian PIAS (protein inhibitor of activated STAT-1 (signal transducer and
activator of transcription-1)) family. This member contains a putative zinc-binding motif and a highly acidic
region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase
II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear
receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.
(provided by RefSeq, Jul 2008)

GeneCards Summary for PIAS1 Gene:
PIAS1 (protein inhibitor of activated STAT, 1) is a protein-coding gene. GO annotations related to this gene include transcription corepressor activity and enzyme binding. An important paralog of this gene is PIAS3.

UniProtKB/Swiss-Prot: PIAS1_HUMAN, O75925
Function: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between
UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation
in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling
pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or
silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional
activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160')
and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its
interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity)

Gene Wiki entry for PIAS1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000015.9  NT_010194.18  NC_018926.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the PIAS1 gene promoter:
         MEF-2A   AP-1   ATF-2   aMEF-2   c-Jun   RSRFC4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidPIAS1 promoter sequence
   Search Chromatin IP Primers for PIAS1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PIAS1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q   Ensembl cytogenetic band:  15q23   HGNC cytogenetic band: 15q

PIAS1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PIAS1 gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15P068346:  view genomic region     (about GC identifiers)

Start:
68,346,517 bp from pter      End:
68,483,096 bp from pter
Size:
136,580 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: PIAS1_HUMAN, O75925 (See protein sequence)
Recommended Name: E3 SUMO-protein ligase PIAS1  
Size: 651 amino acids; 71836 Da
Subunit: Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation (By
similarity). Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer),
following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the
interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle
progression into G(1) phase. Interacts with STAT1. Interacts with CHUK/IKKA; this interaction induces PIAS1
phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5.
Interacts with PML (By similarity)
1 PDB 3D structure from and Proteopedia for PIAS1:
1V66 (3D)    
Secondary accessions: B2RB67 Q147X4 Q99751 Q9UN02

Explore the universe of human proteins at neXtProt for PIAS1: NX_O75925

Explore proteomics data for PIAS1 at MOPED

Post-translational modifications: 

  • Sumoylated1
  • Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to
    preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene1
  • Ubiquitination2 at Lys8, Lys46, Lys315, Lys339, Lys493
  • Modification sites at PhosphoSitePlus

  • See PIAS1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_057250.1  
    ENSEMBL proteins: 
     ENSP00000249636   ENSP00000456721   ENSP00000457698   ENSP00000438574   ENSP00000456558  
    Reactome Protein details: O75925

    PIAS1 Human Recombinant Protein Products:

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    Novus Biologicals PIAS1 Proteins
    Novus Biologicals PIAS1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for PIAS1

     
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    antibodies-online proteins for PIAS1 (8 products) 

     
    antibodies-online peptides for PIAS1

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    antibodies-online antibodies for PIAS1 (58 products) 

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ZMIZ: Zinc fingers, MIZ-type

    4 InterPro protein domains:
     IPR004181 Znf_MIZ
     IPR003034 SAP_dom
     IPR023321 PINIT
     IPR027227 PIAS1

    Graphical View of Domain Structure for InterPro Entry O75925

    ProtoNet protein and cluster: O75925

    2 Blocks protein domains:
    IPB003034 DNA-binding SAP
    IPB004181 Zn-finger


    UniProtKB/Swiss-Prot: PIAS1_HUMAN, O75925
    Domain: The LXXLL motif is a transcriptional coregulator signature
    Domain: The SP-RING-type domain is required for promoting EKLF sumoylation (By similarity)
    Similarity: Belongs to the PIAS family
    Similarity: Contains 1 PINIT domain
    Similarity: Contains 1 SAP domain
    Similarity: Contains 1 SP-RING-type zinc finger


    Find genes that share domains with PIAS1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: PIAS1_HUMAN, O75925
    Function: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between
    UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation
    in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling
    pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or
    silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional
    activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160')
    and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its
    interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity)

         Enzyme Number (IUBMB): EC 6.3.2.-1

         Gene Ontology (GO): Selected molecular function terms (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0003713transcription coactivator activity NAS15572661
    GO:0003714transcription corepressor activity TAS10805787
    GO:0005515protein binding IPI14752048
         
    Find genes that share ontologies with PIAS1           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for PIAS1:
     Increased circadian rhythm amp  Synthetic lethal with Ras 

         3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Pias1):
     growth/size/body  immune system  mortality/aging 

    Find genes that share phenotypes with PIAS1           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Pias1tm1Kes for PIAS1

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PIAS1
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       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for PIAS1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for PIAS1

    miRNA
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    miRTarBase miRNAs that target PIAS1:
    hsa-mir-124-3p (MIRT022246), hsa-mir-424-5p (MIRT003227)

    Block miRNA regulation of human, mouse, rat PIAS1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PIAS1 (see all 21):
    hsa-miR-548j hsa-miR-300 hsa-miR-548i hsa-miR-548a-3p hsa-miR-548a-5p hsa-miR-548d-5p hsa-miR-375 hsa-miR-3148
    SwitchGear 3'UTR luciferase reporter plasmidPIAS1 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat PIAS1

    Gene Editing
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    Clone
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    GenScript: all cDNA clones in your preferred vector: PIAS1 (NM_016166)
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    PIAS1_HUMAN, O75925: Nucleus speckle. Nucleus, PML body (By similarity). Note=Interaction with CSRP2 may induce a
    partial redistribution along the cytoskeleton
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA14752048
    GO:0005654nucleoplasm TAS--
    GO:0016605colocalizes with PML body ISS--
    GO:0016607nuclear speck IEA--

    Find genes that share ontologies with PIAS1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for PIAS1 About   (see all 23)  
    See pathways by source

    SuperPathContained pathways About
    1Jak/Stat Pathway
    Development Thrombopoetin signaling via JAK STAT pathway0.32
    Jak/Stat Pathway0.32
    2Interferon Signaling
    Interferon Signaling0.59
    Interferon gamma signaling0.41
    Cytokine Signaling in Immune system0.59
    Regulation of IFNG signaling0.00
    3Immune response Bacterial infections in normal airways
    Immune response Bacterial infections in normal airways0.78
    Bacterial infections in CF airways0.78
    4Proteolysis Putative SUMO 1 pathway
    DNA damage Role of SUMO in p53 regulation0.33
    Proteolysis Putative SUMO 1 pathway0.33
    5TGF-Beta Pathway
    JAK-STAT Pathway0.57


    Find genes that share SuperPaths with PIAS1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    3 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for PIAS1
        p53 Signaling
    JAK-STAT Pathway
    SUMO Pathway

    1 Cell Signaling Technology (CST) Pathway for PIAS1
        Jak/Stat Pathway

    Selected GeneGo (Thomson Reuters) Pathways for PIAS1 (see all 6)
        Immune response Bacterial infections in normal airways
    Proteolysis Putative SUMO-1 pathway
    Transcription P53 signaling pathway
    DNA damage Role of SUMO in p53 regulation
    Bacterial infections in CF airways

    Selected BioSystems Pathways for PIAS1 (see all 12)
        Integrated Breast Cancer Pathway
    Interferon type I
    TGF-beta Receptor Signaling Pathway
    Androgen receptor signaling pathway
    Interleukin-11 Signaling Pathway


    1 Reactome Pathway for PIAS1
        Regulation of IFNG signaling


    3 Kegg Pathways  (Kegg details for PIAS1):
        Ubiquitin mediated proteolysis
    Jak-STAT signaling pathway
    Hepatitis C

    UniProtKB/Swiss-Prot: PIAS1_HUMAN, O75925
    Pathway: Protein modification; protein sumoylation

        Pathway & Disease-focused RT2 Profiler PCR Arrays including PIAS1: 

              JAK / STAT Signaling Pathway in human mouse rat
              Ubiquitin Ligases in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for PIAS1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for PIAS1 (O759251, 2, 3 ENSP000002496364) via UniProtKB, MINT, STRING, and/or I2D (see all 308)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PRDM1O756261, 2, ENSP000003580924EBI-629434,EBI-948789 MINT-8397177 MINT-8397208 STRING: ENSP00000358092
    TP53P046371, 3, ENSP000002693054EBI-629434,EBI-366083 I2D: score=6 STRING: ENSP00000269305
    UBE2IP632792, 3, ENSP000003248974MINT-7230924 I2D: score=6 STRING: ENSP00000324897
    SMAD7O151052, 3, ENSP000002621584MINT-61806 I2D: score=3 STRING: ENSP00000262158
    PRPF40AO754002, 3MINT-61844 I2D: score=3 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 15):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000122negative regulation of transcription from RNA polymerase II promoter IEA--
    GO:0006351transcription, DNA-templated IEA--
    GO:0007259JAK-STAT cascade TAS10805787
    GO:0007283spermatogenesis IEA--
    GO:0016925protein sumoylation ISS--

    Find genes that share ontologies with PIAS1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for PIAS1

    9 Novoseek inferred chemical compound relationships for PIAS1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    lysine 54.3 1 17066076 (1)
    testosterone 33.5 7 11877418 (2), 14644130 (1), 18656483 (1), 12177000 (1)
    arginine 23 6 19136629 (3), 12171910 (2)
    zinc 21 8 17934332 (4)
    steroid 15.7 1 14644130 (1)
    progesterone 14.6 1 11117529 (1)
    serine 11 1 11773441 (1)
    estrogen 7.87 3 11117529 (1), 14644130 (1), 15666801 (1)
    tyrosine 0 1 12171910 (1)



    Find genes that share compounds with PIAS1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for PIAS1 gene: 
    NM_016166.1  

    Unigene Cluster for PIAS1:

    Protein inhibitor of activated STAT, 1
    Hs.162458  [show with all ESTs]
    Unigene Representative Sequence: AK128379
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000249636(uc010ujx.2 uc002aqz.3) ENST00000564915 ENST00000562190
    ENST00000545237 ENST00000564009 ENST00000567417 ENST00000563996
    miRNA
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    hsa-miR-548j hsa-miR-300 hsa-miR-548i hsa-miR-548a-3p hsa-miR-548a-5p hsa-miR-548d-5p hsa-miR-375 hsa-miR-3148
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      QuantiFast Probe-based Assays in human, mouse, rat PIAS1

    Additional mRNA sequence: 

    AF077951.1 AF167160.1 AK094641.1 AK128379.1 AK314515.1 AY826819.1 BC053685.1 BC104646.1 
    BC118587.1 BC121797.1 EF487997.1 EF487998.1 FJ997900.1 U78524.1 

    15 DOTS entries:

    DT.117380  DT.100777930  DT.95148132  DT.97782925  DT.97843831  DT.100026938  DT.121060474  DT.100019100 
    DT.100026939  DT.100777931  DT.91642277  DT.91754892  DT.91754893  DT.95143477  DT.436306 

    Selected AceView cDNA sequences (see all 352):

    BX098741 BP351615 AI151229 CB139868 AA463666 T50552 BQ720672 AW305107 
    AU121697 AI679413 T16734 BU158952 BE886798 BM505486 AU119223 N24089 
    AA411233 BM552077 AA504633 CD678463 AA608955 AI040228 NM_016166 BM677276 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for PIAS1 (see all 8)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10a · 10b ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^
    SP1:                                      -     -     -                       -                                                                                 
    SP2:                          -     -     -     -     -                       -                                                                                 
    SP3:                          -     -                 -                       -                                                                                 
    SP4:                                                                                                                                                            
    SP5:                          -     -     -     -                                                                                                               

    ExUns: 19 ^ 20
    SP1:  -         
    SP2:  -         
    SP3:            
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for PIAS1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    PIAS1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    PIAS1 Expression
    About this image


    PIAS1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 8) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
    PIAS1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    PIAS1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.162458

    UniProtKB/Swiss-Prot: PIAS1_HUMAN, O75925
    Tissue specificity: Expressed in numerous tissues with highest level in testis

        Pathway & Disease-focused RT2 Profiler PCR Arrays including PIAS1: 
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for PIAS1 gene from Selected species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Pias11 , 5 protein inhibitor of activated STAT 11, 5 90.68(n)1
    98(a)1
      9 (33.91 cM)5
    564691  NM_019663.31  NP_062637.21 
     628800775 
    chicken
    (Gallus gallus)
    Aves PIAS11 protein inhibitor of activated STAT, 1 85.96(n)
    96.38(a)
      427514  NM_001031456.1  NP_001026627.1 
    lizard
    (Anolis carolinensis)
    Reptilia PIAS16
    protein inhibitor of activated STAT, 1
    89(a)
    1 ↔ 1
    GL343467.1(708014-727932)
    African clawed frog
    (Xenopus laevis)
    Amphibia pias1-A2 protein inhibitor of activated STAT, 1 80.05(n)    AY100476.1 
    zebrafish
    (Danio rerio)
    Actinopterygii pias1b6
    PIAS1 (1 of 2)6
    protein inhibitor of activated STAT, 1
    77(a)
    76(a)
    1 ↔ many
    1 ↔ many
    18(19771126-19806075) ENSDARG00000062445
    7(35221111-35256121) ENSDARG00000036094
    fruit fly
    (Drosophila melanogaster)
    Insecta Su(var)2-101 , 3 chromosome condensation DNA binding3
    Suppressor of variegation 2-101
    42(a)3
    51.57(n)1
    49.16(a)1
      2 45A83
    359271  NM_001201959.11  NP_001188888.11 
    worm
    (Caenorhabditis elegans)
    Secernentea W10D5.3d3   -- 37(a)
    (best of 3)
      I(9109234-9115598)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes NFI16
    SIZ16
    SUMO E3 ligase, catalyzes the covalent attachment ...
    SUMO/Smt3 ligase that promotes the attachment of s...
    13(a)
    12(a)
    many ↔ many
    many ↔ many
    XV(628360-630540) YOR156C
    IV(1289406-1292120) YDR409W


    ENSEMBL Gene Tree for PIAS1 (if available)
    TreeFam Gene Tree for PIAS1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for PIAS1 gene
    PIAS32  ZMIZ22  PIAS22  ZMIZ12  PIAS42  
    5 SIMAP similar genes for PIAS1 using alignment to 9 protein entries:     PIAS1_HUMAN (see all proteins):
    DKFZp434O0617    PIAS3    PIAS2    PIAS4    DKFZp727A051

    Find genes that share paralogs with PIAS1           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for PIAS1 (see all 2618)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 15 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs718718641,2
    C--48461850(+) CACAC-/ACTT  
            
    ATATA
    1 -- int10--------
    rs801311341,2
    C--68344847(+) TCTTGA/GCAATG 1 -- int12Minor allele frequency- G:0.09CSA WA 120
    rs1886546171,2
    --68344848(+) CTTGAC/TAATGG 1 -- int10--------
    rs1916679631,2
    --68344890(+) CATGAG/TCATAT 1 -- int10--------
    rs1837542391,2
    --68345004(+) GAGGAA/GAAACC 1 -- int10--------
    rs1422012641,2
    --68345116(+) ATTCGC/TGATTC 1 -- int10--------
    rs1881832821,2
    --68345195(+) TCTTGA/GTAAGT 1 -- int10--------
    rs1806741661,2
    --68345426(+) ATTCAA/GTTGAG 1 -- int10--------
    rs1179056731,2
    C,F--68345525(+) AGGGAC/GTGGCC 1 -- int11Minor allele frequency- G:0.07EA 120
    rs1507279861,2
    C--68345558(+) ATTCAC/TAGCAA 1 -- int10--------

    HapMap Linkage Disequilibrium report for PIAS1 (68346517 - 68483096 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for PIAS1 (see all 19):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv1172926CNV Deletion17803354
    esv1003872CNV Deletion20482838
    esv2749814CNV Deletion23290073
    esv2068352CNV Deletion18987734
    esv2665979CNV Deletion23128226
    esv994874CNV Deletion20482838
    esv2596512CNV Deletion19546169
    esv2749813CNV Deletion23290073
    esv2986CNV Deletion18987735
    esv2670973CNV Deletion23128226

    Human Gene Mutation Database (HGMD): PIAS1
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing PIAS1
    DNA2.0 Custom Variant and Variant Library Synthesis for PIAS1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 603566    OMIM disorders: --


    Find genes that share disorders with PIAS1           About GenesLikeMe

    5 Novoseek inferred disease relationships for PIAS1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    mammary tumor 23.1 2 14500761 (1)
    prostate cancer 22.1 2 14871982 (2)
    hepatitis c chronic 12.6 1 14699505 (1)
    tumors 7.15 7 14871982 (2), 15133049 (2), 15657437 (1), 18755518 (1)
    breast cancer 0 1 17717071 (1)

    Genetic Association Database (GAD): PIAS1
    Human Genome Epidemiology (HuGE) Navigator: PIAS1 (2 documents)

    Export disorders for PIAS1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for PIAS1 gene, integrated from 10 sources (see all 179):
    (articles sorted by number of sources associating them with PIAS1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Inhibition of Stat1-mediated gene activation by PIAS1. (PubMed id 9724754)1, 2, 3, 9 Liu B....Shuai K. (Proc. Natl. Acad. Sci. U.S.A. 1998)
    2. Cloning and characterization of Gu/RH-II binding protein. (PubMed id 9177271)1, 2, 3 Valdez B.C.... Busch H. (Biochem. Biophys. Res. Commun. 1997)
    3. Proinflammatory stimuli induce IKKalpha-mediated phosphorylation of PIAS1 to restrict inflammation and immunity. (PubMed id 17540171)1, 2, 9 Liu B.... Shuai K. (Cell 2007)
    4. NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers. (PubMed id 15133049)1, 2, 9 Okubo S.... Shindo H. (J. Biol. Chem. 2004)
    5. Transcription factor Sp3 is silenced through SUMO modification by PIAS1. (PubMed id 12356736)1, 2, 9 Sapetschnig A.... Suske G. (EMBO J. 2002)
    6. Sumoylation of Mdm2 by protein inhibitor of activated STAT (PIAS) and RanBP2 enzymes. (PubMed id 12393906)1, 2, 9 Miyauchi Y.... Yasuda H. (J. Biol. Chem. 2002)
    7. Members of the PIAS family act as SUMO ligases for c-Jun and p53 and repress p53 activity. (PubMed id 11867732)1, 2, 9 Schmidt D. and Mueller S. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    8. SUMO-1 modification of the C-terminal KVEKVD of Axin is required for JNK activation but has no effect on Wnt signaling. (PubMed id 12223491)1, 2, 9 Rui H.L.... Lin S.C. (J. Biol. Chem. 2002)
    9. Protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis. (PubMed id 10628744)1, 2, 9 Tan J.... French F.S. (Mol. Endocrinol. 2000)
    10. Genetic polymorphisms, their allele combinations and IFN-beta treatment response in Irish multiple sclerosis patients. (PubMed id 19604093)1, 4 O'Doherty C....Vandenbroeck K. (Pharmacogenomics 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 8554 HGNC: 2752 AceView: PIAS1 Ensembl:ENSG00000033800 euGenes: HUgn8554
    ECgene: PIAS1 Kegg: 8554 H-InvDB: PIAS1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for PIAS1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for PIAS1 gene:
    Search GeneIP for patents involving PIAS1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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