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PFKFB3 Gene

protein-coding   GIFtS: 64
GCID: GC10P006186

6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 31 2     6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase2
Renal Carcinoma Antigen NY-REN-562 3     6-Phosphofructo-2-Kinase/Fructose-2, 6-Bisphosphatase2
6PF-2-K/Fru-2,6-P2ase 32 3     6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase 32
6PF-2-K/Fru-2,6-P2ase Brain/Placenta-Type Isozyme2 3     PFK22
iPFK-22 3     Fructose-6-Phosphate,2-Kinase/Fructose-2, 6-Bisphosphatase2
PFK/FBPase 32 3     Inducible 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase2
IPFK22 5     

External Ids:    HGNC: 88741   Entrez Gene: 52092   Ensembl: ENSG000001705257   OMIM: 6053195   UniProtKB: Q168753   

Export aliases for PFKFB3 gene to outside databases

Previous GC identifers: GC10P006189 GC10P006395 GC10P006248 GC10P006226 GC10P006284


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for PFKFB3 Gene:
PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3) is a protein-coding gene. Diseases associated with PFKFB3 include colon adenocarcinoma, and glycogen storage disease. GO annotations related to this gene include 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. An important paralog of this gene is PFKFB1.

UniProtKB/Swiss-Prot: F263_HUMAN, Q16875
Function: Synthesis and degradation of fructose 2,6-bisphosphate

Gene Wiki entry for PFKFB3 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000010.11  NT_008705.17  NC_018921.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the PFKFB3 gene promoter:
         USF1   USF-1:USF-2   POU3F2 (N-Oct-5a)   POU3F2 (N-Oct-5b)   POU3F2   Arnt   c-Ets-1   CREB   deltaCREB   USF-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): PFKFB3 promoter sequence
   Search Chromatin IP Primers for PFKFB3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PFKFB3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10p15.1   Ensembl cytogenetic band:  10p15.1   HGNC cytogenetic band: 10p15.1

PFKFB3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PFKFB3 gene location

GeneLoc information about chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P006186:  view genomic region     (about GC identifiers)

Start:
6,186,843 bp from pter      End:
6,277,508 bp from pter
Size:
90,666 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: F263_HUMAN, Q16875 (See protein sequence)
Recommended Name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3  
Size: 520 amino acids; 59609 Da
Selected PDB 3D structures from and Proteopedia for PFKFB3 (see all 8):
2AXN (3D)        2DWO (3D)        2DWP (3D)        2I1V (3D)        3QPU (3D)        3QPV (3D)    
Secondary accessions: O43622 O75902 Q5VX15 Q5VX18 Q5VX19
Alternative splicing: 3 isoforms:  Q16875-1   Q16875-2   Q16875-3   (Gene prediction based on EST data)

Explore the universe of human proteins at neXtProt for PFKFB3: NX_Q16875

Explore proteomics data for PFKFB3 at MOPED

Post-translational modifications: 

  • Phosphorylation by AMPK stimulates activity1
  • Ubiquitination2 at Lys302
  • Modification sites at PhosphoSitePlus

  • See PFKFB3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001138915.1  NP_001269559.1  NP_004557.1  

    ENSEMBL proteins: 
     ENSP00000369115   ENSP00000369105   ENSP00000433771   ENSP00000434628   ENSP00000436885  
     ENSP00000432356   ENSP00000369111   ENSP00000353712   ENSP00000369100   ENSP00000390285  
     ENSP00000434342   ENSP00000416677   ENSP00000404995   ENSP00000443319   ENSP00000446384  
     ENSP00000369108  
    Reactome Protein details: Q16875

    PFKFB3 Human Recombinant Protein Products:

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    Novus Biologicals PFKFB3 Lysates
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    Sino Biological Cell Lysate for PFKFB3
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    Cloud-Clone Corp. Proteins for PFKFB3

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    Abcam antibodies for PFKFB3 (O60825, Q16875, Q16877)
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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 6):
     IPR016260 Bifunct_6PFK/fruc_bisP_Ptase
     IPR001345 PG/BPGM_mutase_AS
     IPR003094 6Pfruct_kin
     IPR013079 6Phosfructo_kin
     IPR027417 P-loop_NTPase

    Graphical View of Domain Structure for InterPro Entry Q16875

    ProtoNet protein and cluster: Q16875

    2 Blocks protein domains:
    IPB001345 Phosphoglycerate/bisphosphoglycerate mutase
    IPB013079 6-phosphofructo-2-kinase


    UniProtKB/Swiss-Prot: F263_HUMAN, Q16875
    Similarity: In the C-terminal section; belongs to the phosphoglycerate mutase family


    PFKFB3 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: F263_HUMAN, Q16875
    Function: Synthesis and degradation of fructose 2,6-bisphosphate
    Catalytic activity: Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
    Catalytic activity: ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate

         Genatlas biochemistry entry for PFKFB3:
    fructose 2,6-P2 synthesis and degradation,bifunctional enzyme,?,expressed in kidney

         Gene Ontology (GO): 3 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:00038736-phosphofructo-2-kinase activity NAS8830046
    GO:0004331fructose-2,6-bisphosphate 2-phosphatase activity IEA--
    GO:0005524ATP binding IEA--
         
    PFKFB3 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for PFKFB3:
     Decreased 12E8 and total tau p 

         1 MGI mutant phenotype (inferred from 1 allele(MGI details for Pfkfb3):
     mortality/aging 

    PFKFB3 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Pfkfb3tm1Jche for PFKFB3

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PFKFB3
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       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for PFKFB3
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for PFKFB3

    miRNA
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    miRTarBase miRNAs that target PFKFB3:
    hsa-mir-330-3p (MIRT043840), hsa-mir-26b-5p (MIRT028775)

    Block miRNA regulation of human, mouse, rat PFKFB3 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PFKFB3 (see all 52):
    hsa-miR-300 hsa-miR-106a hsa-miR-488 hsa-miR-301a hsa-miR-519a hsa-miR-133a hsa-miR-93 hsa-miR-526b*
    SwitchGear 3'UTR luciferase reporter plasmidPFKFB3 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for PFKFB3
    Predesigned siRNA for gene silencing in human, mouse, rat PFKFB3

    Gene Editing
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    Clone
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    Sino Biological Human cDNA Clone for PFKFB3
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PFKFB3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PFKFB3

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for PFKFB3 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PFKFB3


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol4
    nucleus2
    mitochondrion1
    peroxisome1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005730NOT nucleolus IDA--
    GO:0005829cytosol TAS--

    PFKFB3 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for PFKFB3 About   (see all 11)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Akt Signaling
    Akt Signaling0.60
    2MPS VI - Maroteaux-Lamy syndrome
    Metabolism of carbohydrates0.45
    3Glucose metabolism
    Glucose metabolism0.44
    4Glycolysis and gluconeogenesis short map
    Glycolysis0.40
    5Metabolism
    Metabolism0.38

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for PFKFB3
        AMPK Enzyme Complex Pathway
    Akt Signaling

    1 Cell Signaling Technology (CST) Pathway for PFKFB3
        Glucose / Energy Metabolism

    2 BioSystems Pathways for PFKFB3
        AMPK signaling
    HIF-1-alpha transcription factor network


    1 Reactome Pathway for PFKFB3
        Glycolysis


    2 Kegg Pathways  (Kegg details for PFKFB3):
        Fructose and mannose metabolism
    HIF-1 signaling pathway


    PFKFB3 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including PFKFB3: 
              Hypoxia Signaling Pathway in human mouse rat
              Diabetes in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for PFKFB3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for PFKFB3 (Q168753 ENSP000003691004) via UniProtKB, MINT, STRING, and/or I2D (see all 42)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TNFP013753, ENSP000003986984I2D: score=1 STRING: ENSP00000398698
    ENSG00000204490P013753I2D: score=1 
    ENSG00000206439P013753I2D: score=1 
    ENSG00000223952P013753I2D: score=1 
    ENSG00000228321P013753I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005975carbohydrate metabolic process TAS--
    GO:0006000fructose metabolic process IEA--
    GO:0006003fructose 2,6-bisphosphate metabolic process IEA--
    GO:0006006glucose metabolic process TAS--
    GO:0006096glycolytic process TAS--

    PFKFB3 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for PFKFB3 (F263)

    7 HMDB Compounds for PFKFB3    About this table
    CompoundSynonyms CAS #PubMed Ids
    2-Phosphoglyceric acid2-(dihydrogen phosphate)Glycerate (see all 16)2553-59-5--
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    Beta-D-Fructose 6-phosphatebeta-D-Fructose 6-phosphate (see all 2)----
    D-Fructose 2,6-bisphosphateD-Fructose 2,6-bisphosphate (see all 6)----
    PhosphateNFB Orthophosphate (see all 13)14265-44-2--
    WaterDihydrogen oxide (see all 2)7732-18-5--

    Selected Novoseek inferred chemical compound relationships for PFKFB3 gene (see all 19)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    fructose-6-phosphate 79.4 4 1833982 (1), 15994045 (1), 2163613 (1), 8394741 (1)
    fructose 57.4 2 20006513 (1), 10072580 (1)
    glycerol 3-phosphate 48.1 2 7499445 (1), 8394741 (1)
    glucose 45.3 14 19473963 (2), 18039179 (2), 15640157 (1), 19937972 (1) (see all 11)
    phosphoenolpyruvate 41.6 3 7499445 (1), 2163613 (1), 8394741 (1)
    lactate 36.5 5 16912547 (1), 18202014 (1), 17805487 (1), 2163613 (1) (see all 5)
    citrate 34.1 3 10521487 (1), 7499445 (1), 2163613 (1)
    atp 32.7 2 18202014 (1), 12417335 (1)
    vegf 26.1 5 15640157 (1), 17143338 (1), 15925437 (1), 16311927 (1) (see all 5)
    phosphocreatine 25 1 12417335 (1)



    PFKFB3 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for PFKFB3 gene (3 alternative transcripts): 
    NM_001145443.1  NM_001282630.1  NM_004566.3  

    Unigene Cluster for PFKFB3:

    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
    Hs.195471  [show with all ESTs]
    Unigene Representative Sequence: NM_004566
    17 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000379789(uc001ijd.3 uc010qaw.2 uc009xik.3 uc009xil.3)
    ENST00000317350 ENST00000461744 ENST00000467491 ENST00000490474 ENST00000477914
    ENST00000379785 ENST00000360521 ENST00000379775(uc001ije.3 uc001ijf.3)
    ENST00000450232 ENST00000487989 ENST00000475881 ENST00000414237 ENST00000441697
    ENST00000536985(uc009xii.3) ENST00000540253 ENST00000379782

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    Selected qRT-PCR Assays for microRNAs that regulate PFKFB3 (see all 52):
    hsa-miR-300 hsa-miR-106a hsa-miR-488 hsa-miR-301a hsa-miR-519a hsa-miR-133a hsa-miR-93 hsa-miR-526b*
    SwitchGear 3'UTR luciferase reporter plasmidPFKFB3 3' UTR sequence
    Inhib. RNA
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      QuantiTect SYBR Green Assays in human, mouse, rat PFKFB3
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    Additional mRNA sequence: 

    AF056320.1 AF109735.1 AJ227868.1 AJ272438.1 AJ272439.1 AJ272440.1 AJ295747.1 AK291263.1 
    AK303039.1 AK304450.1 BC037258.1 BC040482.1 D49817.1 L77662.1 

    15 DOTS entries:

    DT.92439698  DT.92439691  DT.95148380  DT.99995463  DT.100775884  DT.75117867  DT.92439689  DT.91746482 
    DT.95095678  DT.95377617  DT.40112378  DT.121274363  DT.121274440  DT.75138328  DT.95107470 

    Selected AceView cDNA sequences (see all 213):

    AW471347 AW768640 BE326961 AI952793 AW449507 AJ272440 BQ003837 BU194193 
    AA811308 H88301 T32788 BX642776 AI580224 AJ272439 AJ227868 AI648689 
    AI565523 T08556 BM505274 AW130192 BU193109 AW779837 AW572037 AI567474 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for PFKFB3 (see all 10)    About this scheme

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11a · 11b ^ 12a · 12b · 12c ^ 13a · 13b · 13c ^ 14a · 14b ^ 15 ^
    SP1:                                                                          -     -           -           -                             -     -               
    SP2:        -     -     -     -     -                                         -                 -           -                 -     -     -     -     -         
    SP3:                                                                                                                          -     -     -     -               
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 16a · 16b ^ 17 ^ 18a · 18b ^ 19a · 19b · 19c · 19d
    SP1:              -           -                           
    SP2:  -     -     -           -                           
    SP3:              -     -     -                           
    SP4:                                                      
    SP5:              -                                       


    ECgene alternative splicing isoforms for PFKFB3

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    PFKFB3 expression in normal human tissues (normalized intensities)      PFKFB3 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AACTTTTGGC
    PFKFB3 Expression
    About this image


    PFKFB3 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 15) fully expand
     
     Bone (Muscoskeletal System)    fully expand to see all 5 entries
             Zeugopod Long Bone
     
     Inner Cell Mass (Early Embryonic Tissues)    fully expand to see all 2 entries
             Human embryonic stem cells (family)
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Cerebral Cortex
     
     Cartilage (Muscoskeletal System)
             Meckel's Cartilage
     
     Neural Tube (Nervous System)
             Telencephalon
    PFKFB3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    PFKFB3 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.195471

    UniProtKB/Swiss-Prot: F263_HUMAN, Q16875
    Tissue specificity: Ubiquitous

        Pathway & Disease-focused RT2 Profiler PCR Arrays including PFKFB3: 
              Hypoxia Signaling Pathway in human mouse rat
              Diabetes in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for PFKFB3 gene from Selected species (see all 18)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Pfkfb31 , 5 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 less1, 5 88.34(n)1
    97.64(a)1
      2 (8.73 cM)5
    1707681  NM_001177757.11  NP_001171228.11 
     114714305 
    chicken
    (Gallus gallus)
    Aves PFKFB31 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 less 80.26(n)
    87.06(a)
      418247  XM_416472.4  XP_416472.2 
    lizard
    (Anolis carolinensis)
    Reptilia PFKFB36
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatas...
    86(a)
    1 ↔ 1
    5(566235-598337)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia pfkfb31 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 less 71.29(n)
    79.74(a)
      100216216  NM_001142184.1  NP_001135656.1 
    zebrafish
    (Danio rerio)
    Actinopterygii BC060925.12   -- 75.97(n)    BC060925.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Pfrx6
    6-phosphofructo-2-kinase
    37(a)
    1 → many
    X(19389538-19403390)
    worm
    (Caenorhabditis elegans)
    Secernentea Y110A7A.6b3
    Y110A7A.61
    Y110A7A.61 56(a)
    (best of 3)3
    56.25(n)1
    57.69(a)1
      I(5131526-5133910)3
    1721471  NM_059128.51  NP_491529.21 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes FBP266
    Fructose-2,6-bisphosphatase, required for glucose ...
    44(a)
    1 → many
    X(129285-130643) YJL155C
    barley
    (Hordeum vulgare)
    Liliopsida AV910103.12   -- 69.6(n)    AV910103.1 


    ENSEMBL Gene Tree for PFKFB3 (if available)
    TreeFam Gene Tree for PFKFB3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for PFKFB3 gene
    PFKFB12  PFKFB22  PFKFB42  
    Selected SIMAP similar genes for PFKFB3 using alignment to 12 protein entries:     F263_HUMAN (see all proteins) (see all similar genes):
    PFK2    HINFP    PACRG    PARK2    SPG7    VPS33A
    ZNF114    ZNF620    ADCK1    ARL6IP5    IRF2    FTSJ2
    MRPL45    NKIRAS2    TUBA1C    PTPLAD1    ZNF554    PFKFB4

    PFKFB3 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for PFKFB3 (see all 2744)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 10 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs353385991,2
    C--6144168(+) TTTTT-/TTTT  
      
    /TTTTT
    AGACG
    1 -- int11NA 2
    rs586496561,2
    C--6153570(+) AAAAA-/A/AAA 
            
    GAAAA
    1 -- int11NA 2
    rs171363991,2
    F--6170226(+) TGGACAAGGAC/-
            
    GAGGT
    2 -- us2k1 int12Minor allele frequency- -:0.17MN EA 356
    rs18870281,2
    C,F,A,H--6184858(-) CCAAAA/GCCCTT 1 -- us2k12Minor allele frequency- G:0.15WA EA 238
    rs9431231,2
    C,H--6184862(-) GCTTCT/CAAAAC 1 -- us2k112Minor allele frequency- C:0.04WA NA CSA EA 375
    rs9431221,2
    C,F,A,H--6184909(-) GGGCCG/ATGGCT 1 -- us2k14Minor allele frequency- A:0.09NS WA NA EA 478
    rs9431211,2
    C,F,A,H--6184913(-) GATGGG/TGCCGT 1 -- us2k13Minor allele frequency- T:0.14WA NA EA 358
    rs1396177101,2
    C--6184961(+) GCGGCA/GGTGGC 1 -- us2k10--------
    rs115991161,2
    C,F,H--6184982(+) CAGGCG/AGGATG 1 -- us2k12Minor allele frequency- A:0.13NA 124
    rs115934741,2
    C,F,H--6185016(+) TGGCCT/CTGGAT 1 -- us2k15Minor allele frequency- C:0.09NA CSA WA EA 364

    HapMap Linkage Disequilibrium report for PFKFB3 (6186843 - 6277508 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for PFKFB3 (see all 27):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv994076CNV Deletion20482838
    dgv100e201CNV Deletion23290073
    esv2626974CNV Deletion19546169
    esv1353057CNV Deletion17803354
    esv2731929CNV Deletion23290073
    esv271270CNV Insertion20981092
    nsv5665CNV Insertion18451855
    nsv437094CNV Loss16327808
    nsv894816CNV Loss21882294
    dgv124n67CNV Loss20364138

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing PFKFB3
    DNA2.0 Custom Variant and Variant Library Synthesis for PFKFB3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 605319    OMIM disorders: --

    18 diseases for PFKFB3:    About MalaCards
    colon adenocarcinoma    glycogen storage disease    hypoxia    ischemia
    astrocytoma    adenocarcinoma    chronic lymphocytic leukemia    renal cell carcinoma
    obesity    pancreatic cancer    pancreatitis    alzheimer's disease
    leukemia    hepatitis    endotheliitis    prostatitis
    cerebritis    neuronitis


    PFKFB3 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    6 Novoseek inferred disease relationships for PFKFB3 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    colon adenocarcinoma 45.9 5 12007784 (2), 12384552 (1)
    cancer 26.8 21 16115917 (4), 17143338 (3), 12384552 (3), 12963966 (1) (see all 12)
    solid tumor 3.29 1 16912547 (1)
    tumors 2.7 9 16115917 (3), 12384552 (2), 12963966 (1), 10077634 (1) (see all 5)
    leukemia 0 1 16912547 (1)
    ischemia 0 3 12392881 (3)

    Genetic Association Database (GAD): PFKFB3
    Human Genome Epidemiology (HuGE) Navigator: PFKFB3 (1 document)

    Export disorders for PFKFB3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for PFKFB3 gene, integrated from 10 sources (see all 110):
    (articles sorted by number of sources associating them with PFKFB3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning, expression, and chromosomal localization of a ubiquitously expressed human 6-phosphofructo-2-kinase/ fructose-2, 6- bisphosphatase gene (PFKFB3). (PubMed id 10072580)1, 2, 3, 9 Manzano A.... Bartrons R. (Cytogenet. Cell Genet. 1998)
    2. The third human isoform of 6-phosphofructo-2-kinase/fructose-2,6- bisphosphatase (PFKFB3) map position 10p14-p15. (PubMed id 9146922)1, 2, 3, 9 Nicholl J....Watts C.K. (Chromosome Res. 1997)
    3. Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy. (PubMed id 16316985)1, 2, 9 Kim S.G....Lee Y.H. (J. Biol. Chem. 2006)
    4. An inducible gene product for 6-phosphofructo-2-kinase with an AU- rich instability element: role in tumor cell glycolysis and the Warburg effect. (PubMed id 10077634)1, 2, 9 Chesney J.... Bucala R. (Proc. Natl. Acad. Sci. U.S.A. 1999)
    5. A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. (PubMed id 17499765)1, 2, 9 Kim S.G....Lee Y.H. (J. Mol. Biol. 2007)
    6. Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: transition state and the C-terminal function. (PubMed id 22275052)1, 2 Cavalier M.C....Lee Y.H. (Proteins 2012)
    7. A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease. (PubMed id 16385451)1, 4 Grupe A....Goate A. (Am. J. Hum. Genet. 2006)
    8. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    9. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    10. Antigens recognized by autologous antibody in patients with renal- cell carcinoma. (PubMed id 10508479)1, 2 Scanlan M.J....Old L.J. (Int. J. Cancer 1999)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 5209 HGNC: 8874 AceView: PFKFB3 Ensembl:ENSG00000170525 euGenes: HUgn5209
    ECgene: PFKFB3 Kegg: 5209 H-InvDB: PFKFB3

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for PFKFB3 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for PFKFB3 gene:
    Search GeneIP for patents involving PFKFB3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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