PDPK1 Gene
protein-coding GIFtS: 72
GCID: GC16P002587
|
|
3-phosphoinositide dependent protein kinase-1
| |
Aliases
for PDPK1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| 3-Phosphoinositide Dependent Protein Kinase-11 2 | | PkB Kinase Like Gene 12 | | PDK11 2 3 5 | | PkB-Like 12 | | EC 2.7.11.13 8 | | HPDK11 | | 3-Phosphoinositide-Dependent Protein Kinase 12 | | EC 2.7.118 | | PRO04612 | | |
Export aliases for PDPK1 gene to outside databasesPrevious GC identifers: GC16M002672 GC16P002634 GC16P002527 |
Summaries
for PDPK1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: PDPK1_HUMAN, O15530Function: Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGCfamily of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal proteinS6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent proteinkinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3),p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signalsfrom insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstreamtargets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negativelyregulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor,phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and thetranslocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptionalactivity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. Thetyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controlsproliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry andCa(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and isinvolved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dualeffector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development byregulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cellgrowth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-Bactivation in macrophages. Isoform 3 is catalytically inactive
 summary
for PDPK1: Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the
function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt,
ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main
effector of PDK1.PDK1 also phosphorylates several members of the AGC (automatic gain control) family of
protein kinases. PDK1 phosphorylates substrates either constitutively or in response to a cellular agonist.
Gene Wiki entry for PDPK1 (Phosphoinositide-dependent kinase-1)
|
Genomic Views
for PDPK1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000016.9 NC_018927.1 NT_010393.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the PDPK1 gene promoter:
XBP-1 CUTL1 Tal-1beta E47 CREB POU2F1 POU2F1a ARP-1 ITF-2
Other transcription factors
Search SABiosciences Chromatin IP Primers for PDPK1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PDPK1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 16p13.3 Ensembl cytogenetic band: 16p13.3 HGNC cytogenetic band: 16p13.3PDPK1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 16 GeneLoc Exon Structure GeneLoc location for GC16P002587: view genomic region
(about GC identifiers)
Start:
|
2,587,965 bp from pter |
End:
|
2,653,189 bp from pter |
Size:
|
65,225 bases |
Orientation:
|
plus strand |
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Proteins
for PDPK1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: PDPK1_HUMAN, O15530 (See
protein sequence)Recommended Name: 3-phosphoinositide-dependent protein kinase 1 Size: 556 amino acids; 63152 Da
Subunit: Homodimer in its autoinhibited state. Active as monomer. Interacts with NPRL2, PPARG, PAK1, PTK2B, GRB14, PKN1(via C-terminus), STRAP and IKKB. The Tyr-9 phosphorylated form interacts with SRC, RASA1 and CRK (via their SH2domains). Interacts with SGK3 in a phosphorylation-dependent manner. The tyrosine-phosphorylated form interacts withPTPN6. The Ser-241 phosphorylated form interacts with YWHAH and YWHAQ. Binds INSR in response to insulin. Interacts(via PH domain) with SMAD3, SMAD4 and SMAD7. Interacts with PKN2; the interaction stimulates PDPK1autophosphorylation, its PI(3,4,5)P3-dependent kinase activity toward 'Ser-473' of AKT1 but also activates its kinaseactivity toward PRKCD and PRKCZ
Subcellular location: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion.Note=Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane followinginsulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization tothe cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation atSer-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. TheSer-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cellsit is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronalPDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizeswith PTK2B in focal adhesions after angiotensin II stimulation
Sequence caution: Sequence=BAD93072.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
6/51 PDB 3D structures from  and Proteopedia  for PDPK1 (see all 51):1H1W (3D)
1OKY (3D)
1OKZ (3D)
1UU3 (3D)
1UU7 (3D)
1UU8 (3D)
Secondary accessions: H0Y4Z0 Q59EH6 Q6FI20 Q8IV52 Q9BRD5Alternative splicing: 5 isoforms: O15530-1 O15530-2 O15530-3 O15530-4 O15530-5 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for PDPK1: NX_O15530
Post-translational modifications:
Phosphorylation on Ser-241 in the activation loop is required for full activity. PDPK1 itself can autophosphorylateSer-241, leading to its own activation. Autophosphorylation is inhibited by the apoptotic C-terminus cleavage productof PKN2 (By similarity). Tyr-9 phosphorylation is critical for stabilization of both PDPK1 and the PDPK1/SRC complexvia HSP90-mediated protection of PDPK1 degradation. Angiotensin II stimulates the tyrosine phosphorylation of PDPK1 invascular smooth muscle in a calcium- and SRC-dependent manner. Phosphorylated on Tyr-9, Tyr-373 and Tyr-376 by INSR inresponse to insulin. Palmitate negatively regulates autophosphorylation at Ser-241 and palmitate-inducedphosphorylation at Ser-529 and Ser-501 by PKC/PRKCQ negatively regulates its ability to phosphorylate PKB/AKT1.Phosphorylation at Thr-354 by MELK partially inhibits kinase activity, the inhibition is cooperatively enhanced byphosphorylation at Ser-394 and Ser-398 by MAP3K51
Autophosphorylated; autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2 (Bysimilarity)1
Monoubiquitinated in the kinase domain, deubiquitinated by USP41
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O15530
4/11 DME Specific Peptides for PDPK1 (O15530) (see all 11)
PDPK1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (3 alternative transcripts):
NP_001248745.1 NP_002604.1 NP_112558.2
ENSEMBL proteins: ENSP00000344220 ENSP00000455492 ENSP00000395357 ENSP00000268673 ENSP00000455025 ENSP00000455684 ENSP00000373876 ENSP00000455551 ENSP00000455438 ENSP00000346895 Reactome Protein details: O15530
Human Recombinant Protein Products:
Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6): About this table
PDPK1 for ontologies About GeneDecksing
PDPK1 Antibody Products:
Assay Products for PDPK1: |
Protein
Domains /
Families
for PDPK1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
PDPK1 for domains About GeneDecksing
5/6 InterPro domains/families (see all 6):Graphical View of Domain Structure for InterPro Entry O15530ProtoNet protein and cluster: O15530 1 Blocks protein family: IPB001849 Pleckstrin-like
UniProtKB/Swiss-Prot: PDPK1_HUMAN, O15530Domain: The PH domain plays a pivotal role in the localization and nuclear import of PDPK1 and is also essential forits homodimerizationSimilarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamilySimilarity: Contains 1 PH domainSimilarity: Contains 1 protein kinase domain |
Function
for PDPK1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: PDPK1_HUMAN, O15530Function: Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGCfamily of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal proteinS6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent proteinkinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3),p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signalsfrom insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstreamtargets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negativelyregulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor,phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and thetranslocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptionalactivity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. Thetyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controlsproliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry andCa(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and isinvolved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dualeffector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development byregulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cellgrowth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-Bactivation in macrophages. Isoform 3 is catalytically inactiveCatalytic activity: ATP + a protein = ADP + a phosphoproteinEnzyme regulation: Homodimerization regulates its activity by maintaining the kinase in an autoinhibitory conformation.NPRL2 down-regulates its activity by interfering with tyrosine phosphorylation at the Tyr-9, Tyr-373 and Tyr-376residues. The 14-3-3 protein YWHAQ acts as a negative regulator by association with the residues surrounding theSer-241 residue. STRAP positively regulates its activity by enhancing its autophosphorylation and by stimulating itsdissociation from YWHAQ. SMAD2, SMAD3, SMAD4 and SMAD7 also positively regulate its activity by stimulating itsdissociation from YWHAQ. Activated by phosphorylation on Tyr-9, Tyr-373 and Tyr-376 by INSR in response to insulinInduction: Stimulated by insulin, and the oxidants hydrogen peroxide and peroxovanadate Genatlas biochemistry entry for PDPK1:3-phosphoinositide (PtdIns(3,4,5)P3,PtdIns(3,4)P2)-dependent kinase 1,ubiquitously expressed,homolog to DrosophilaDSTPK61 kinase,likely mediating the activation of protein-kinase B AKT/PKB by insulin or growth factors
Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PDPK1 (see all 5)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PDPK1 (see all 3)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): PDPK1 (NM_002613) | |  | Sino Biological Human cDNA Clone for PDPK1 | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PDPK1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDPK1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDPK1 |
Gene Ontology (GO): 5/8 molecular function terms (GO ID links to tree view) (see all 8): About this table
PDPK1 for ontologies About GeneDecksing
3 GenomeRNAi human phenotypes for PDPK1:
Animal Models:
Mouse knock-out Pdpk1tm1.2Mlw for PDPK1
15/22 MGI mutant phenotypes (inferred from 11 alleles ) (MGI details for Pdpk1) (see all 22):
PDPK1 for phenotypes About GeneDecksing
|
Pathways & Interactions
for PDPK1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/103 super-pathways (see all 103) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Signaling by FGFR | | | 2 | IL-9 Signaling Pathways | | | 3 | Development_CNTF receptor signaling | | | 4 | PIP3 activates AKT signaling | | | 5 | IRS-related events | |
Pathway sources
See GeneCards unified pathways
Show all pathways
5/38 EMD Millipore Pathways for PDPK1 (see all 38)
5/9  R&D Systems Pathways for PDPK1 (see all 9)
5/38 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for PDPK1 (see all 38)
1 
Cell Signaling Technology (CST) Pathway for PDPK1
3 Tocris Bioscience Pathways for PDPK1
5/44  GeneGo (Thomson Reuters) Pathways for PDPK1 (see all 44)
5/28  BioSystems Pathways for PDPK1 (see all 28)
5/62 
Reactome Pathways for PDPK1 (see all 62)
5/8 
Kegg Pathways (Kegg details for PDPK1) (see all 8):
PDPK1 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for PDPK1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/445 Interacting proteins for PDPK1 (O155301, 2, 3 ENSP000003442204) via UniProtKB, MINT, STRING, and/or I2D (see all 445)About this table
Gene Ontology (GO): 5/32 biological process terms (GO ID links to tree view) (see all 32): About this table
PDPK1 for ontologies About GeneDecksing
|
Drugs & Compounds
for PDPK1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
PDPK1 for compounds About GeneDecksing
 |
Enzo Life Sciences drugs & compounds for PDPK1 |
Compounds for PDPK1 available from Tocris Bioscience About this table
| Compound | Action |
CAS
# |
|---|
| PS 48 | PDK1 activator | [1180676-32-7] | | GSK 2334470 | Highly potent, selective PDK1 | [1227911-45-6] | | BAG 956 | Dual PI 3-kinase and PDK1 inhibitor | [853910-02-8] | | BX 795 | PDK1 inhibitor | [702675-74-9] |
3 HMDB Compounds for PDPK1 About this table
10/13 DrugBank Compounds for PDPK1 (see all 13) About this table
| Compound | Synonyms |
CAS
# | Type | Actions | PubMed Ids |
|---|
| Celecoxib | -- | 169590-42-5 | target | inhibitor | 16060857 14973075 16887935 16455452 15205346 | | 3-[1-(3-Aminopropyl)-1h-Indol-3-Yl]-4-(1-Methyl-1h-Indol-3-Yl)-1h-Pyrrole-2,5-Dione | -- | -- | target | -- | 17139284 17016423 10592235 | | 7-Hydroxystaurosporine | -- | 112953-11-4 | target | -- | 17139284 17016423 | | Inositol 1,3,4,5-Tetrakisphosphate | -- | -- | target | -- | 17139284 17016423 | | Phosphonoserine | -- | -- | target | -- | 17139284 17016423 | | Staurosporine | -- | 62996-74-1 | target | -- | 17139284 17016423 | | 1-{2-OXO-3-[(1R)-1-(1H-PYRROL-2-YL)ETHYL]-2H-INDOL-5-YL}UREA | -- | -- | target | -- | 10592235 | | 10,11-dimethoxy-4-methyldibenzo[c,f]-2,7-naphthyridine-3,6-diamine | -- | -- | target | -- | 10592235 | | 2-(1H-imidazol-1-yl)-9-methoxy-8-(2-methoxyethoxy)benzo[c][2,7]naphthyridin-4-amine | -- | -- | target | -- | 10592235 | | 3-(1H-INDOL-3-YL)-4-(1-{2-[(2S)-1-METHYLPYRROLIDINYL]ETHYL}-1H-INDOL-3-YL)-1H-PYRROLE-2,5-DIONE | -- | -- | target | -- | 10592235 |
10/41 Novoseek chemical compound relationships for PDPK1 gene (see all 41) About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| phosphatidylinositol |
79.2 |
47 |
12183455 (2), 9799739 (1), 11060301 (1), 15520204 (1) (see all 32) |
| phosphatidylinositol-3,4,5-trisphosphate |
78.4 |
9 |
9768361 (1), 9637919 (1), 11882383 (1), 15537704 (1) (see all 9) |
| phosphoinositide |
78 |
76 |
11897568 (3), 19061482 (2), 9637919 (2), 18790745 (2) (see all 40) |
| threonine |
71.1 |
25 |
15567163 (2), 15743412 (2), 14570903 (1), 17617409 (1) (see all 17) |
| 7-hydroxystaurosporine |
70.8 |
5 |
12892559 (2), 16284058 (1), 17429623 (1) |
| rapamycin |
68.4 |
10 |
19061482 (1), 19153210 (1), 16787414 (1), 12668683 (1) (see all 9) |
| wortmannin |
67.4 |
11 |
11054117 (2), 11907830 (1), 10995762 (1), 11096081 (1) (see all 7) |
| ly294002 |
65.2 |
4 |
18174238 (2), 11907830 (1), 14645548 (1) |
| pkcs |
62.5 |
8 |
17617409 (2), 11368419 (1), 16923125 (1), 17392460 (1) |
| serine |
61.2 |
21 |
15567163 (2), 14570903 (1), 17617409 (1), 19276999 (1) (see all 16) |
Search CenterWatch for drugs/clinical trials and news about PDPK1
|
Transcripts
for PDPK1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for PDPK1 gene (3 alternative transcripts): NM_001261816.1 NM_002613.4 NM_031268.5 Unigene Cluster for PDPK1: 3-phosphoinositide dependent protein kinase-1 Hs.459691 [show with all ESTs]Unigene Representative Sequence: NM_00261318 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000342085(uc002cqs.3 uc002cqt.3 uc010bsn.3 uc002cqu.3)
ENST00000566659 ENST00000441549 ENST00000268673 ENST00000462923 ENST00000474706
ENST00000492021 ENST00000570136(uc010uwe.1) ENST00000389224 ENST00000461815
ENST00000491073(uc002cqv.1) ENST00000460496 ENST00000464702 ENST00000471311
ENST00000478708 ENST00000569721 ENST00000561962 ENST00000354836
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PDPK1 (see all 5)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PDPK1 (see all 3)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): PDPK1 (NM_002613) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PDPK1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDPK1 |
Additional cDNA sequence: AB209835.1 AF017995.1 AF111845.2 AJ785968.1 AK093687.1 AK095507.1 AK131060.1 AK222581.1 AL157446.1 BC000789.1 BC006339.2 BC012103.1 BC012540.1 BC033494.1 BC040324.1 CR536517.1 Y15056.1 24 DOTS entries: DT.100785196 DT.449221 DT.100025648 DT.95374105 DT.91962365 DT.91931583 DT.92434118 DT.95157696 DT.95157697 DT.101983627 DT.70103679 DT.92039625 DT.100719463 DT.102830651 DT.120706556 DT.95150360 DT.99930183 DT.120706438 DT.40202251 DT.95357717 DT.101969931 DT.120706432 DT.120706520 DT.414093 24/379 AceView cDNA sequences (see all 379): BU517342 BU688266 AI222823 NM_002613 BX283735 AI202572 AI206540 AI934960 BU687739 AW248835 AI753561 AL710195 NM_031268 N98243 CB159271 BC000789 BM928596 BE550498 BG055217 BE463748 AW194773 AI418787 BM312785 AI494378
GeneLoc Exon Structure
5/8 Alternative Splicing Database (ASD) splice patterns (SP) for PDPK1 (see all 8) About this scheme
| ExUns: | 1a | · | 1b | ^ | 2a | · | 2b | ^ | 3 | ^ | 4 | ^ | 5a | · | 5b | ^ | 6 | ^ | 7a | · | 7b | ^ | 8a | · | 8b | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13a | · | 13b | · | 13c | ^ | 14 | ^ | 15 | ^ | 16 | ^ | 17 |
|---|
|
| SP1: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | | | - | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for PDPK1
|
Expression
for PDPK1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| PDPK1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TAGAGTGTAA
About this image
See PDPK1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for PDPK1
SOURCE GeneReport for Unigene cluster: Hs.459691
UniProtKB/Swiss-Prot: PDPK1_HUMAN, O15530Tissue specificity: Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased indiseased tissue compared with normal tissue from lung, liver, colon and breast
SABiosciences Expression via Pathway-Focused PCR Arrays including PDPK1 (see all 8):
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PDPK1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat PDPK1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PDPK1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PDPK1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDPK1 |
Orthologs
for PDPK1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for PDPK1 gene from 8/29 species (see all 29) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
PDPK11 |
3-phosphoinositide dependent protein kinase-1 |
79.11(n)
91.3(a) |
|
416588 NM_001012529.1 NP_001012547.1 |
lizard
(Anolis carolinensis) |
Reptilia |
PDPK16 |
-- |
87(a) |
1 ↔ 1 |
GL343612.1(216531-228653) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.158492 |
Xenopus laevis transcribed sequence with moderate similarity more |
79.03(n) |
|
BX843060.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
Dr.143832 |
Transcribed sequence with moderate similarity to protein more |
79.78(n) |
|
BQ450455.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
Pk61C3
Pdk11 |
protein amino acid phosphorylation protein
serine/threonine more3
Phosphoinositide-dependent kinase 11 |
46(a)3
53.25(n)1
50.85(a)1 |
|
61B13
380171 NM_167791.21 NP_728472.11 |
worm
(Caenorhabditis elegans) |
Secernentea |
W04B5.53
pdk-11 |
protein kinase3
Protein PDK-11 |
41(a)3
51.02(n)1
41.92(a)1 |
|
III(2483062-2490008)3
1804751 NM_001029571.11 NP_001024742.11 |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
PDK11 |
3'-phosphoinositide-dependent protein kinase 1 |
50.82(n)
44.29(a) |
|
830330 NM_120533.4 NP_568138.1 |
rice
(Oryza sativa) |
Liliopsida |
Os01g08728001 |
hypothetical protein |
49.58(n)
43.19(a) |
|
4324953 NM_001051483.1 NP_001044948.1 |
ENSEMBL Gene Tree for PDPK1 (if available)
TreeFam Gene Tree for PDPK1 (if available) |
Paralogs
for PDPK1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for PDPK1 gene
18/45 SIMAP similar genes for PDPK1 using alignment to 8 protein entries: PDPK1_HUMAN (see all proteins)
(see all similar genes):PkB-like 2 PDPK2 PkB-like 1 STK11 PRKACB KIN27
MARK2 MARK3 STK32A RPS6KA3 RPS6KA1 PRKACA
RPS6KA5 RPS6KA2 PRKAA1 CIT SGK3 AKT3
PDPK1 for paralogs About GeneDecksing
3 Pseudogenes.org Pseudogenes for PDPK1
PGOHUM00000262269 PGOHUM00000262270 PGOHUM00000262271
|
Genomic Variants
for PDPK1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 16 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for PDPK1 (2587965 - 2653189 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 10 variations for PDPK1
10 CNVs: 7098 4918 5329 61489 66919 66917 4919 3988 32074 66918
Human Gene Mutation Database (HGMD): PDPK1
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PDPK1 |
|
Disorders
/ Diseases
for PDPK1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
PDPK1 for disorders About GeneDecksing
OMIM gene information: 605213
OMIM disorders: --
20/43  diseases for PDPK1 (see all 43): About MalaCardsprogressive transformation of germinal centers estrogen-receptor negative breast cancer insulin resistance papillary thyroid carcinoma
chronic lymphocytic leukemia lymphocytic leukemia pituitary tumor thyroid carcinoma
breast cancer retinoblastoma gout prostate cancer
aldosteronism endometrial cancer lung adenocarcinoma glioblastoma
leiomyoma rhabdomyosarcoma thyroiditis prostate adenocarcinoma
10/14 Novoseek disease relationships for PDPK1 gene (see all 14) About this table
| Disease |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| cancer |
36.3 |
25 |
18542064 (3), 20197379 (2), 19602588 (2), 19061482 (1) (see all 14) |
| tumors |
24.3 |
36 |
20197379 (3), 19602588 (3), 16243031 (2), 19291795 (1) (see all 18) |
| glioblastoma |
16.6 |
3 |
11102805 (1), 19591923 (1) |
| retinoblastoma |
15.3 |
1 |
19706764 (1) |
| prostate cancer |
13.2 |
1 |
19822882 (1) |
| metastasis |
4.34 |
1 |
19531564 (1) |
| insulin resistance |
2.78 |
3 |
12882908 (1), 20018868 (1) |
| breast cancer |
2.04 |
26 |
16782806 (4), 19531564 (3), 16288304 (3), 19602588 (3) (see all 11) |
| leukemia |
0 |
1 |
16670263 (1) |
| necrosis |
0 |
5 |
19920149 (1) |
Human Genome Epidemiology (HuGE) Navigator: PDPK1 (4 documents)
Export disorders for PDPK1 gene to outside databases
|
Publications
for PDPK1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for PDPK1 gene, integrated from 9 sources (see all 418):
(articles sorted by number of sources associating them with PDPK1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Protein kinase B kinases that mediate phosphatidylinositol 3,4,5- trisphosphate-dependent activation of protein kinase B. (PubMed id 9445477)1, 2, 3 Stephens L.R.... Hawkins P.T. (1998)
- Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B alpha. (PubMed id 9094314)1, 2, 3 Alessi D.R.... Cohen P. (1997)
- Regulation of 3-phosphoinositide-dependent protein kinase-1 (PDK1) by Src involves tyrosine phosphorylation of PDK1 and Src homology 2 domain binding. (PubMed id 18024423)1, 2, 9 Yang K.J....Park J. (2008)
- 3-phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase. (PubMed id 9368760)1, 2, 9 Alessi D.R.... Bownes M. (1997)
- PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2. (PubMed id 10226025)1, 2, 9 Balendran A....Alessi D.R. (1999)
- 3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins. (PubMed id 17327236)1, 2, 9 Seong H.A....Ha H. (2007)
- Identification of tyrosine phosphorylation sites on 3- phosphoinositide-dependent protein kinase-1 (PDK1) and their role in regulating kinase activity. (PubMed id 11481331)1, 2, 9 Park J....Hemmings B.A. (2001)
- Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. (PubMed id 9637919)1, 2, 9 Anderson K.E.... Hawkins P.T. (1998)
- TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling. (PubMed id 18616680)1, 2, 9 Kurata A.... Fujita N. (2008)
- Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation. (PubMed id 15743829)1, 2, 9 Scheid M.P....Woodgett J.R. (2005)
|
External Searches for PDPK1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing PDPK1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing PDPK1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PDPK1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for PDPK1 gene:
Search GeneIP for patents involving PDPK1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for PDPK1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | OriGene Antibodies for PDPK1 | | Browse OriGene shRNA RFPs | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PDPK1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PDPK1 | | | OriGene Protein Over-expression Lysate for PDPK1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for PDPK1 | | OriGene 3'-UTR Clone for PDPK1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PDPK1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PDPK1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for PDPK1 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for PDPK1 | | OriGene Custom Protein Services for PDPK1 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat PDPK1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PDPK1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PDPK1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PDPK1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PDPK1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PDPK1 |
| |
 |  |  |  | |
| | Antibodies & Assays for PDPK1 (PDK1) |
| | | | Tocris compounds for PDPK1 |
| |  |  |  |  | | | | |
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 |
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| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDPK1 |
|  |  |  | | | | ThermoFisher Antibody for PDPK1 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDPK1 |
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