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PDK1 Gene

protein-coding   GIFtS: 69
GCID: GC02P173420

Pyruvate Dehydrogenase Kinase, Isozyme 1

(Previous names: pyruvate dehydrogenase kinase, isoenzyme 1)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Pyruvate Dehydrogenase Kinase, Isozyme 11 2     Mitochondrial Pyruvate Dehydrogenase, Lipoamide, Kinase Isoenzyme 12
Pyruvate Dehydrogenase Kinase, Isoenzyme 11 2     PDHK13
PDH Kinase 12 3     Pyruvate Dehydrogenase Kinase Isoform 13
EC 2.7.11.23 8     EC 2.7.118
[Pyruvate Dehydrogenase (Acetyl-Transferring)] Kinase Isozyme 1,
Mitochondrial2
     

External Ids:    HGNC: 88091   Entrez Gene: 51632   Ensembl: ENSG000001522567   OMIM: 6025245   UniProtKB: Q151183   

Export aliases for PDK1 gene to outside databases

Previous GC identifers: GC02P171473 GC02P172081 GC02P173384 GC02P173623 GC02P173246 GC02P173129 GC02P165298


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for PDK1 Gene:
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation
of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels
in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of
PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced
transcript variants have been found for this gene. (provided by RefSeq, Jun 2013)

GeneCards Summary for PDK1 Gene:
PDK1 (pyruvate dehydrogenase kinase, isozyme 1) is a protein-coding gene. GO annotations related to this gene include protein serine/threonine kinase activity and pyruvate dehydrogenase (acetyl-transferring) kinase activity. An important paralog of this gene is BCKDK.

UniProtKB/Swiss-Prot: PDK1_HUMAN, Q15118
Function: Serine/threonine kinase that plays a key role in regulation of glucose and fatty acid metabolism and
homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate
dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle,
down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an
important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects
cells against apoptosis in response to hypoxia and oxidative stress

Gene Wiki entry for PDK1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000002.11  NT_005403.18  NC_018913.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the PDK1 gene promoter:
         CREB   p53   AP-1   deltaCREB   STAT3   NF-kappaB1   NF-kappaB   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidPDK1 promoter sequence
   Search Chromatin IP Primers for PDK1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PDK1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q31.1   Ensembl cytogenetic band:  2q31.1   HGNC cytogenetic band: 2q31.1

PDK1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PDK1 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P173420:  view genomic region     (about GC identifiers)

Start:
173,420,101 bp from pter      End:
173,489,823 bp from pter
Size:
69,723 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: PDK1_HUMAN, Q15118 (See protein sequence)
Recommended Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial precursor  
Size: 436 amino acids; 49244 Da
Subunit: Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and
is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase
(E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3)
3 PDB 3D structures from and Proteopedia for PDK1:
2Q8F (3D)        2Q8G (3D)        2Q8H (3D)    
Secondary accessions: B2R6T1 D3DPD8

Explore the universe of human proteins at neXtProt for PDK1: NX_Q15118

Explore proteomics data for PDK1 at MOPED

Post-translational modifications: 

  • Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in
    cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and
    Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for PDK1 (Q15118) (see all 13)
     VYVPSHL  TPSVQLV  KNAMRAT  SDRGGGV 


    See PDK1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001265478.1  NP_002601.1  

    ENSEMBL proteins: 
     ENSP00000399558   ENSP00000406608   ENSP00000282077   ENSP00000376352   ENSP00000386985  
     ENSP00000413629   ENSP00000399160   ENSP00000388366   ENSP00000438567   ENSP00000437502  
    Reactome Protein details: Q15118

    PDK1 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant PDK1 Protein
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    OriGene MassSpec for PDK1
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    Novus Biologicals PDK1 Proteins
    Novus Biologicals PDK1 Lysate
    Sino Biological Recombinant Protein for PDK1
    Sino Biological Cell Lysate for PDK1
    ProSpec Recombinant Protein for PDK1
    Cloud-Clone Corp. Proteins for PDK1

     
    Search eBioscience for Proteins for PDK1 

     
    antibodies-online proteins for PDK1 (17 products) 

     
    antibodies-online peptides for PDK1

    PDK1 Antibody Products:

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    LSBio Antibodies in human, mouse, rat for PDK1
    antibodies-online antibodies for PDK1 (168 products) 

    PDK1 Assay Products:

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    Cloud-Clone Corp. ELISAs for PDK1
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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    3 InterPro protein domains:
     IPR005467 Sig_transdc_His_kinase_core
     IPR003594 HATPase_ATP-bd
     IPR018955 BCDHK/PDK_N

    Graphical View of Domain Structure for InterPro Entry Q15118

    ProtoNet protein and cluster: Q15118

    1 Blocks protein domain: IPB003594 ATP-binding region

    UniProtKB/Swiss-Prot: PDK1_HUMAN, Q15118
    Similarity: Belongs to the PDK/BCKDK protein kinase family
    Similarity: Contains 1 histidine kinase domain


    Find genes that share domains with PDK1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: PDK1_HUMAN, Q15118
    Function: Serine/threonine kinase that plays a key role in regulation of glucose and fatty acid metabolism and
    homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate
    dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle,
    down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an
    important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects
    cells against apoptosis in response to hypoxia and oxidative stress
    Catalytic activity: ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase
    (acetyl-transferring)] phosphate
    Enzyme regulation: Activity is enhanced by binding to the pyruvate dehydrogenase subunit DLAT. Inhibited by
    AZD7545; this compound interferes with DLAT binding and thereby inhibits kinase activity. Inhibited by
    dichloroacetate and radicicol
    Induction: Up-regulated via the HIF1A signaling pathway in response to hypoxia

         Enzyme Numbers (IUBMB): EC 2.7.11.21 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity IDA17683942
    GO:0004674protein serine/threonine kinase activity IEA--
    GO:0004740pyruvate dehydrogenase (acetyl-transferring) kinase activity IMP--
    GO:0005515protein binding IPI17110931
    GO:0005524ATP binding IEA--
         
    Find genes that share ontologies with PDK1           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for PDK1:
     Decreased viability of wild-ty  Increases Tamoxifen sensitivit 

         1 MGI phenotypic allele for Pdk1 (no phenotypes)

    Find genes that share phenotypes with PDK1           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PDK1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for PDK1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for PDK1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for PDK1

    miRNA
    Products:
        
    miRTarBase miRNAs that target PDK1:
    hsa-mir-26b-5p (MIRT029318), hsa-let-7b-5p (MIRT032356), hsa-mir-155-5p (MIRT020770), hsa-mir-375 (MIRT000055)

    Block miRNA regulation of human, mouse, rat PDK1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PDK1 (see all 32):
    hsa-miR-640 hsa-miR-128 hsa-miR-301a hsa-miR-500a hsa-miR-155 hsa-miR-130b hsa-miR-301b hsa-miR-27a
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for PDK1
    Predesigned siRNA for gene silencing in human, mouse, rat PDK1

    Gene Editing
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    DNA2.0 Custom Protein Engineering Service for PDK1

    Clone
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    OriGene clones in human, mouse for PDK1 (see all 7)
    OriGene ORF clones in mouse, rat for PDK1
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    GenScript: all cDNA clones in your preferred vector: PDK1 (NM_002610)
    Sino Biological Human cDNA Clone for PDK1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PDK1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDK1
    Addgene plasmids for PDK1 

    Cell Line
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDK1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    PDK1_HUMAN, Q15118: Mitochondrion matrix
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    mitochondrion5

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005739mitochondrion TAS7499431
    GO:0005759mitochondrial matrix TAS--
    GO:0005967mitochondrial pyruvate dehydrogenase complex IEA--

    Find genes that share ontologies with PDK1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for PDK1 About   (see all 12)  
    See pathways by source

    SuperPathContained pathways About
    1Citric acid cycle (TCA cycle)
    Pyruvate metabolism and Citric Acid (TCA) cycle0.49
    Pyruvate metabolism0.00
    Regulation of pyruvate dehydrogenase (PDH) complex0.00
    2TNF Signaling
    Death Receptor Signaling0.50
    NF-kB (NFkB) Pathway0.46
    3Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
    The citric acid (TCA) cycle and respiratory electron transport0.71
    4ErbB signaling pathway
    ErbB signaling pathway0.52
    5Nanog in Mammalian ESC Pluripotency
    eNOS Signaling0.48


    Find genes that share SuperPaths with PDK1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for PDK1
        eNOS Signaling

    1 Cell Signaling Technology (CST) Pathway for PDK1
        Glucose / Energy Metabolism

    4 BioSystems Pathways for PDK1
        DNA damage response (only ATM dependent)
    ErbB signaling pathway
    EPO Receptor Signaling
    Integrated Pancreatic Cancer Pathway

    2 Sino Biological Pathways for PDK1
        Death Receptor Signaling
    NF-kB (NFkB) Pathway

    1 Reactome Pathway for PDK1
        Regulation of pyruvate dehydrogenase (PDH) complex

    1 PharmGKB Pathway for PDK1
        Celecoxib Pathway, Pharmacodynamics

    1 Kegg Pathway  (Kegg details for PDK1):
        HIF-1 signaling pathway

        Pathway & Disease-focused RT2 Profiler PCR Arrays including PDK1: 
              PI3K-AKT Signaling Pathway in human mouse rat
              Hypoxia Signaling Pathway in human mouse rat
              Glucose Metabolism in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for PDK1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for PDK1 (Q151181, 2, 3 ENSP000002820774) via UniProtKB, MINT, STRING, and/or I2D (see all 55)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PKN2Q165131, 2, 3, ENSP000003595524EBI-7016221,EBI-2511350 MINT-3390515 MINT-3390536 MINT-3390583 MINT-3390627 MINT-3390561 MINT-3390605 I2D: score=2 STRING: ENSP00000359552
    RPS6KB1P234432, 3, ENSP000002255774MINT-3390512 I2D: score=5 STRING: ENSP00000225577
    PDHA1P085592, 3, ENSP000003943824MINT-8227846 I2D: score=2 STRING: ENSP00000394382
    EPAS1Q998142, 3, ENSP000002637344MINT-3378519 I2D: score=1 STRING: ENSP00000263734
    SGK3Q96BR12, 3MINT-2881442 MINT-2881402 I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006006glucose metabolic process IEA--
    GO:0006090pyruvate metabolic process TAS--
    GO:0006468protein phosphorylation IMP--
    GO:0008283cell proliferation IMP18541534
    GO:0008631intrinsic apoptotic signaling pathway in response to oxidative stress IMP18541534

    Find genes that share ontologies with PDK1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for PDK1

    3 HMDB Compounds for PDK1    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    Hydrogen phosphateBiphosphate (see all 10)14066-19-4--

    2 DrugBank Compounds for PDK1    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    4-[(3-CHLORO-4-{[(2R)-3,3,3-TRIFLUORO-2-HYDROXY-2-METHYLPROPANOYL]AMINO}PHENYL)SULFONYL]-N,N-DIMETHYLBENZAMIDE-- --target--10592235
    Dichloroacetic AcidDCA (see all 2)79-43-6targetinhibitor2554095

    Selected Novoseek inferred chemical compound relationships for PDK1 gene (see all 20)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    pyruvate 75.9 39 11485553 (2), 16517406 (2), 16517405 (2), 17785433 (2) (see all 21)
    phosphatidylinositol-3,4,5-trisphosphate 63.4 3 10841774 (1), 16505097 (1), 15494023 (1)
    phosphatidylinositol 60.5 9 12738763 (2), 11313398 (1), 15572689 (1), 12553797 (1) (see all 6)
    rapamycin 52.4 3 17369395 (1), 18937252 (1)
    acetyl-coa 51 1 16517405 (1)
    threonine 49.9 4 12738763 (1), 12374740 (1), 19663778 (1)
    serine 46.3 5 12738763 (1), 19379206 (1), 15077109 (1), 19663778 (1)
    ly294002 45.1 1 12417714 (1)
    lactate 36.8 3 18811052 (1), 20039079 (1)
    phosphoinositide 36.4 2 19025518 (1), 20200230 (1)



    Find genes that share compounds with PDK1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for PDK1 gene (2 alternative transcripts): 
    NM_001278549.1  NM_002610.4  

    Unigene Cluster for PDK1:

    Pyruvate dehydrogenase kinase, isozyme 1
    Hs.470633  [show with all ESTs]
    Unigene Representative Sequence: NM_002610
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000443353 ENST00000436490 ENST00000282077 ENST00000392571(uc002uhq.1)
    ENST00000410055(uc002uhs.3 uc010zeb.2) ENST00000431718 ENST00000416991
    ENST00000439519 ENST00000466437 ENST00000543905(uc010zdz.1 uc010zea.1)
    ENST00000544863
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    hsa-miR-640 hsa-miR-128 hsa-miR-301a hsa-miR-500a hsa-miR-155 hsa-miR-130b hsa-miR-301b hsa-miR-27a
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      QuantiFast Probe-based Assays in human, mouse, rat PDK1

    Additional mRNA sequence: 

    AK056110.1 AK294194.1 AK302321.1 AK304388.1 AK312700.1 BC039158.1 DQ234350.1 L42450.1 

    10 DOTS entries:

    DT.91757915  DT.120966878  DT.120966929  DT.121643378  DT.95165272  DT.100024294  DT.120966729  DT.120966826 
    DT.100774079  DT.91757914 

    Selected AceView cDNA sequences (see all 112):

    BM463262 BM809134 BF056615 BP369196 BQ441659 BG939341 NM_002610 BQ049669 
    AA641881 CR594556 AU118504 AI753813 BC039158 AA284869 BM837775 BQ050786 
    CB322020 AI755010 AI276949 BF447288 AA703075 BX116008 BX485353 BQ185162 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for PDK1 (see all 8)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11 ^ 12 ^ 13a · 13b · 13c · 13d ^ 14
    SP1:                                      -                                   -                                               -         
    SP2:                                                                          -                                                         
    SP3:                                      -                                   -                                                         
    SP4:                                -     -                                   -                                                         
    SP5:                                                              -     -     -                                                         


    ECgene alternative splicing isoforms for PDK1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    PDK1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GATCAACCCT
    PDK1 Expression
    About this image


    PDK1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 9) fully expand
     
     Gonad (Reproductive System)    fully expand to see all 3 entries
             Primordial Germ Cells Primitive Gonad
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Primary Oocyte Primary Follicle
     
     Kidney (Urinary System)
             Metanephros
     
     Eye (Sensory Organs)
             Lens
     
     Neural Tube (Nervous System)
             Metencephalon
    PDK1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    PDK1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.470633

    UniProtKB/Swiss-Prot: PDK1_HUMAN, Q15118
    Tissue specificity: Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and
    pancreas

        Pathway & Disease-focused RT2 Profiler PCR Arrays including PDK1: 
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for PDK1 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Pdk11 , 5 pyruvate dehydrogenase kinase, isoenzyme 11, 5 86.64(n)1
    92.17(a)1
      2 (42.84 cM)5
    2280261  NM_172665.51  NP_766253.21 
     718732245 
    chicken
    (Gallus gallus)
    Aves PDK11 pyruvate dehydrogenase kinase, isozyme 1 82.23(n)
    89.09(a)
      425972  NM_001031352.3  NP_001026523.2 
    lizard
    (Anolis carolinensis)
    Reptilia PDK16
    pyruvate dehydrogenase kinase, isozyme 1
    85(a)
    1 ↔ 1
    GL343353.1(116882-137540)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia pdk11 pyruvate dehydrogenase kinase, isozyme 1 76.2(n)
    83.29(a)
      100497838  XM_002934618.2  XP_002934664.2 
    zebrafish
    (Danio rerio)
    Actinopterygii pdk11 pyruvate dehydrogenase kinase, isozyme 1 72.63(n)
    78.52(a)
      555840  XM_678484.3  XP_683576.2 
    fruit fly
    (Drosophila melanogaster)
    Insecta Pdk3 pyruvate dehydrogenase (lipoamide)
    kinase
    56(a)     --
    worm
    (Caenorhabditis elegans)
    Secernentea ZK370.53 Phosphoprotein 50(a)   III(8809765-8813134)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes PKP16
    Mitochondrial protein kinase involved in negative ...
    24(a)
    1 → many
    IX(275108-276292) YIL042C


    ENSEMBL Gene Tree for PDK1 (if available)
    TreeFam Gene Tree for PDK1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for PDK1 gene
    BCKDK2  PDK42  PDK22  PDK32  
    4 SIMAP similar genes for PDK1 using alignment to 9 protein entries:     PDK1_HUMAN (see all proteins):
    PDK2    PDK4    PDK3    BCKDK

    Find genes that share paralogs with PDK1           About GenesLikeMe


    2 Pseudogenes.org Pseudogenes for PDK1
    PGOHUM00000236421 PGOHUM00000241875


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for PDK1 (see all 1011)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1399404601,2
    --173418834(+) TTCACA/GCAAAT 1 -- us2k10--------
    rs1446421481,2
    --173418843(+) ATTAAC/TGTTCA 1 -- us2k10--------
    rs1474486061,2
    --173418935(+) TAAAGA/TGACAA 1 -- us2k10--------
    rs101786541,2
    C,F,H--173418939(+) GTGACA/CAGACT 1 -- us2k111Minor allele frequency- C:0.13NS EA NA WA 786
    rs779331331,2
    C,F--173418973(+) AAGGAA/GGGAGA 1 -- us2k11Minor allele frequency- G:0.02WA 118
    rs102025501,2
    C,F,H--173418990(+) AGGAGG/AAGGCA 1 -- us2k1 trp317Minor allele frequency- A:0.08NS EA NA WA 2338
    rs748070971,2
    C,F--173419009(+) GGCTGG/AGGTAG 1 -- us2k11Minor allele frequency- A:0.04EA 120
    rs1851963731,2
    --173419097(+) GGTCCC/TGCAGA 1 -- us2k10--------
    rs779532411,2
    C--173419115(+) TGGGAA/GGAAGA 1 -- us2k10--------
    rs1167946881,2
    C,F--173419284(+) ACCAGG/ATTTGA 1 -- us2k11Minor allele frequency- A:0.01NA 120

    HapMap Linkage Disequilibrium report for PDK1 (173420101 - 173489823 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for PDK1:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv3035CNV Loss18451855
    dgv4363n71CNV Loss21882294
    dgv4364n71CNV Loss21882294

    1 Site Specific Mutation Identification with PCR Assay for PDK1:
    Cosmic IdAA Change
    48650p.C421Y
    2 Copy Number PCR Panels containing PDK1:
    Oncogenes & Tumor Suppressor Genes 384HC
    Kinases & Phosphatases
    SeqTarget long-range PCR primers for resequencing PDK1
    DNA2.0 Custom Variant and Variant Library Synthesis for PDK1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 602524    OMIM disorders: --


    Find genes that share disorders with PDK1           About GenesLikeMe

    3 Novoseek inferred disease relationships for PDK1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    cancer 27.6 9 18811052 (2), 16826425 (1), 18204440 (1), 18542064 (1) (see all 6)
    tumors 3.33 9 16513837 (1), 18780147 (1), 15736311 (1), 17517659 (1) (see all 6)
    leukemia 0 1 17255356 (1)

    Genetic Association Database (GAD): PDK1
    Human Genome Epidemiology (HuGE) Navigator: PDK1 (9 documents)

    Export disorders for PDK1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for PDK1 gene, integrated from 10 sources (see all 146):
    (articles sorted by number of sources associating them with PDK1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Diversity of the pyruvate dehydrogenase kinase gene family in humans. (PubMed id 7499431)1, 2, 3, 9 Gudi R.... Popov K.M. (J. Biol. Chem. 1995)
    2. Distinct structural mechanisms for inhibition of pyruvate dehydrogenase kinase isoforms by AZD7545, dichloroacetate, and radicicol. (PubMed id 17683942)1, 2, 9 Kato M.... Chuang D.T. (Structure 2007)
    3. Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells. (PubMed id 18541534)1, 2, 9 McFate T.... Verma A. (J. Biol. Chem. 2008)
    4. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PubMed id 20877624)1, 4 Hendrickson S.L....O'Brien S.J. (PLoS ONE 2010)
    5. Identification of type 2 diabetes-associated combination of SNPs using support vector machine. (PubMed id 20416077)1, 4 Ban H.J....Park K.J. (BMC Genet. 2010)
    6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    8. PDK1 mediates growth factor-induced Ral-GEF activation by a kinase-independent mechanism. (PubMed id 11889038)1, 9 Tian X....Feig L.A. (EMBO J. 2002)
    9. Regulation of pyruvate dehydrogenase activity through phosphorylation at multiple sites. (PubMed id 11485553)1, 9 Kolobova E....Popov K.M. (Biochem. J. 2001)
    10. HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxia. (PubMed id 16517405)1, 9 Kim J.W....Dang C.V. (Cell Metab. 2006)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 5163 HGNC: 8809 AceView: PDK1 Ensembl:ENSG00000152256 euGenes: HUgn5163
    ECgene: PDK1 Kegg: 5163 H-InvDB: PDK1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for PDK1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for PDK1 gene:
    Search GeneIP for patents involving PDK1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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