Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



PDE8A Gene

protein-coding   GIFtS: 67
GCID: GC15P085523

Phosphodiesterase 8A

  See PDE8A-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Phosphodiesterase 8A1 2
HsT195502
CAMP-Specific Cyclic Nucleotide Phosphodiesterase 8A2
High Affinity CAMP-Specific And IBMX-Insensitive 3',5'-Cyclic
Phosphodiesterase 8A2
EC 3.1.4.533
EC 3.1.4.178

External Ids:    HGNC: 87931   Entrez Gene: 51512   Ensembl: ENSG000000734177   OMIM: 6029725   UniProtKB: O606583   

Export aliases for PDE8A gene to outside databases

Previous GC identifers: GC15P081636 GC15P078745 GC15P083110 GC15P083254 GC15P083324 GC15P061611


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for PDE8A Gene:
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8
subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of
cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein
plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding
different isoforms have been found for this gene.(provided by RefSeq, Jul 2011)

GeneCards Summary for PDE8A Gene:
PDE8A (phosphodiesterase 8A) is a protein-coding gene. Diseases associated with PDE8A include granulomatous amebic encephalitis. GO annotations related to this gene include phosphorelay response regulator activity and 3',5'-cyclic-nucleotide phosphodiesterase activity. An important paralog of this gene is PDE4D.

UniProtKB/Swiss-Prot: PDE8A_HUMAN, O60658
Function: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological
processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of
germ cell development

summary for PDE8A Gene:
Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the
hydrolysis of 3' cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or
cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by
controlling the rate of their degradation. There are 11 subtypes of PDEs, named PDE1-11; PDE4, 7 and 8
selectively degrade cAMP, PDE5, 6 and 9 selectively degrade cGMP and PDE1, 2, 3, 10 and 11 degrade both
cyclic nucleotides. PDEs are expressed ubiquitously, with each subtype having a specific tissue
distribution. These enzymes are involved in many signal transduction pathways and their functions include
vascular smooth muscle proliferation and contraction, cardiac contractility, platelet aggregation, hormone
secretion, immune cell activation, and they are involved in learning and memory.

Gene Wiki entry for PDE8A Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000015.9  NT_010194.18  NC_018926.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the PDE8A gene promoter:
         Bach1   p53   AML1a   E47   GATA-1   AREB6   POU2F1   POU2F1a   Pax-4a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidPDE8A promoter sequence
   Search Chromatin IP Primers for PDE8A

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PDE8A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q25.3   Ensembl cytogenetic band:  15q25.3   HGNC cytogenetic band: 15q25.3

PDE8A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PDE8A gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15P085523:  view genomic region     (about GC identifiers)

Start:
85,523,671 bp from pter      End:
85,682,376 bp from pter
Size:
158,706 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: PDE8A_HUMAN, O60658 (See protein sequence)
Recommended Name: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A  
Size: 829 amino acids; 93304 Da
Cofactor: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a
preference for magnesium and/or manganese ions
Sequence caution: Sequence=AAL18612.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=AAL18613.1;
Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=AAL18614.1; Type=Erroneous translation; Note=Wrong
choice of CDS; Sequence=BAG54458.1; Type=Miscellaneous discrepancy; Note=Intron retention; Sequence=EAX01967.1;
Type=Erroneous gene model prediction;
4 PDB 3D structures from and Proteopedia for PDE8A:
1LHQ (3D)        1LXX (3D)        3ECM (3D)        3ECN (3D)    
Secondary accessions: B3KXE6 H0YMZ7 Q6P9H3 Q969I1 Q96PC9 Q96PD0 Q96PD1 Q96T71 Q9UMB7 Q9UMC3
Alternative splicing: 6 isoforms:  O60658-1   O60658-2   O60658-3   O60658-4   O60658-5   O60658-6   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for PDE8A: NX_O60658

Explore proteomics data for PDE8A at MOPED

Post-translational modifications: 

  • Phosphorylated at Ser-359 by PKA under elevated cAMP conditions, this enhances catalytic activity1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • 2 DME Specific Peptides for PDE8A (O60658)
     HDVDHPG  IDMVLAT 


    See PDE8A Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001230066.1  NP_002596.1  NP_775656.1  

    ENSEMBL proteins: 
     ENSP00000311453   ENSP00000453808   ENSP00000340679   ENSP00000432309   ENSP00000431976  
     ENSP00000452907   ENSP00000454094   ENSP00000378056  
    Reactome Protein details: O60658

    PDE8A Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for PDE8A
    OriGene Custom MassSpec
    OriGene Custom Protein Services for PDE8A
    GenScript Custom Purified and Recombinant Proteins Services for PDE8A
    Novus Biologicals PDE8A Proteins
    Novus Biologicals PDE8A Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for PDE8A

     
    Search eBioscience for Proteins for PDE8A 

    PDE8A Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for PDE8A
    OriGene Custom Antibody Services for PDE8A
    Novus Biologicals PDE8A Antibodies
    Abcam antibodies for PDE8A
    Cloud-Clone Corp. Antibodies for PDE8A
    ThermoFisher Antibodies for PDE8A
    LSBio Antibodies in human, mouse, rat for PDE8A

    PDE8A Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for PDE8A
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for PDE8A
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for PDE8A
    Cloud-Clone Corp. CLIAs for PDE8A
    Search eBioscience for ELISAs for PDE8A 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    PDE: Phosphodiesterases

    IUPHAR Guide to PHARMACOLOGY protein family classification: PDE8A
    Phosphodiesterases, 3',5'-cyclic nucleotide

    Selected InterPro protein domains (see all 7):
     IPR002073 PDEase_catalytic_dom
     IPR013767 PAS_fold
     IPR000014 PAS
     IPR023174 PDEase_CS
     IPR023088 PDEase

    Graphical View of Domain Structure for InterPro Entry O60658

    ProtoNet protein and cluster: O60658

    3 Blocks protein domains:
    IPB000014 PAS domain
    IPB013767 PAS fold
    IPB013938 3'5'-cyclic nucleotide phosphodiesterase PDE8


    UniProtKB/Swiss-Prot: PDE8A_HUMAN, O60658
    Domain: Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal
    regulatory domain
    Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily
    Similarity: Contains 1 PAC (PAS-associated C-terminal) domain
    Similarity: Contains 1 PAS (PER-ARNT-SIM) domain


    Find genes that share domains with PDE8A           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: PDE8A_HUMAN, O60658
    Function: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological
    processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of
    germ cell development
    Catalytic activity: Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
    Enzyme regulation: Inhibited by dipyridimole. Insensitive to selective PDE inhibitors including rolipram and
    zaprinast as well as to the non-selective inhibitor, IBMX. Unaffected by cGMP

         Enzyme Numbers (IUBMB): EC 3.1.4.172 EC 3.1.4.531

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000156phosphorelay response regulator activity IEA--
    GO:0003824catalytic activity ----
    GO:00041143',5'-cyclic-nucleotide phosphodiesterase activity NAS9618252
    GO:00041153',5'-cyclic-AMP phosphodiesterase activity IEA--
    GO:0004871signal transducer activity ----
         
    Find genes that share ontologies with PDE8A           About GenesLikeMe


    Phenotypes:
         4 MGI mutant phenotypes (inferred from 1 allele(MGI details for Pde8a):
     behavior/neurological  homeostasis/metabolism  integument  nervous system 

    Find genes that share phenotypes with PDE8A           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PDE8A
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for PDE8A

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for PDE8A
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for PDE8A

    miRNA
    Products:
        
    miRTarBase miRNAs that target PDE8A:
    hsa-mir-183-5p (MIRT047034)

    Block miRNA regulation of human, mouse, rat PDE8A using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PDE8A (see all 34):
    hsa-miR-376b hsa-miR-199a-3p hsa-miR-513a-5p hsa-miR-25 hsa-miR-488 hsa-miR-133a hsa-miR-92b hsa-miR-616
    SwitchGear 3'UTR luciferase reporter plasmidPDE8A 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for PDE8A
    Predesigned siRNA for gene silencing in human, mouse, rat PDE8A

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for PDE8A

    Clone
    Products:
         
    OriGene clones in human, mouse for PDE8A (see all 11)
    OriGene ORF clones in mouse, rat for PDE8A
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): PDE8A (NM_002605)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PDE8A
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDE8A

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for PDE8A
    Browse ESI BIO Cell Lines and PureStem Progenitors for PDE8A 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDE8A


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol4
    cytoskeleton1
    golgi apparatus1
    mitochondrion1
    nucleus1
    plasma membrane1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575cellular_component ----
    GO:0005829cytosol TAS--
    GO:0070062extracellular vesicular exosome IDA19056867

    Find genes that share ontologies with PDE8A           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for PDE8A About    
    See pathways by source

    SuperPathContained pathways About
    1Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.58
    GPCR downstream signaling0.90
    2Circadian entrainment
    Morphine addiction0.53
    3G Protein Signaling Pathways
    G Protein Signaling Pathways0.44
    4Purine metabolism (KEGG)
    Purine metabolism0.38
    5G alpha (s) signalling events
    G alpha (s) signalling events


    Find genes that share SuperPaths with PDE8A           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for PDE8A
        G Protein Signaling Pathways

    1 Reactome Pathway for PDE8A
        G alpha (s) signalling events


    2 Kegg Pathways  (Kegg details for PDE8A):
        Purine metabolism
    Morphine addiction

    UniProtKB/Swiss-Prot: PDE8A_HUMAN, O60658
    Pathway: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for PDE8A
    Interactions:

        Search GeneGlobe Interaction Network for PDE8A

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for PDE8A (O606583 ENSP000003114534) via UniProtKB, MINT, STRING, and/or I2D (see all 74)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    NFKB1P198383I2D: score=1 
    NFKB2Q006533I2D: score=1 
    NFKBIAP259633I2D: score=1 
    NFKBIBQ156533I2D: score=1 
    RELQ048643I2D: score=1 
    About this table

    Gene Ontology (GO): 5 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000160phosphorelay signal transduction system ----
    GO:0006198cAMP catabolic process IEA--
    GO:0006355regulation of transcription, DNA-templated IEA--
    GO:0007165signal transduction ----
    GO:0009187cyclic nucleotide metabolic process NAS9618252

    Find genes that share ontologies with PDE8A           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Compounds for PDE8A available from Tocris Bioscience    About this table
    CompoundAction CAS #
    CilostamidePDE3 inhibitor[68550-75-4]
    RolipramPDE4 inhibitor[61413-54-5]
    IBMXPDE inhibitor (non-selective)[28822-58-4]
    Sildenafil citrate Orally active, potent PDE5 inhibitor [171599-83-0]
    (R)-(-)-RolipramPDE4 inhibitor. More active enantiomer of rolipram (Cat. No. 0905)[85416-75-7]

    7 HMDB Compounds for PDE8A    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine monophosphate5'-AMP (see all 28)61-19-8--
    Cyclic AMPCyclic AMP (see all 19)60-92-4--
    Cyclic GMP3',5'-cyclic GMP (see all 13)7665-99-8--
    Guanosine monophosphate5'-GMP (see all 14)85-32-5--
    MagnesiumMagnesium (see all 2)7439-95-4--
    Manganesemanganese 7439-96-5--
    WaterDihydrogen oxide (see all 2)7732-18-5--

    2 DrugBank Compounds for PDE8A    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    3-ISOBUTYL-1-METHYLXANTHINE-- --target--10592235
    KetotifenKetotifen Fumarate (see all 5)34580-14-8targetinhibitor1702263

    1 IUPHAR Ligand for PDE8A (PDE8A)    About this table
    LigandTypeActionAffinityPubmed IDs
    dipyridamole
    InhibitorInhibition5.19618252

    3 Novoseek inferred chemical compound relationships for PDE8A gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    vinpocetine 79.8 1 9618252 (1)
    ibmx 72.1 3 9618252 (2)
    cgmp 51.2 1 9618252 (1)



    Find genes that share compounds with PDE8A           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for PDE8A gene (6 alternative transcripts): 
    NM_001243137.1  NM_002605.2  NM_173454.1  NM_173455.1  NM_173456.1  NM_173457.1  

    Unigene Cluster for PDE8A:

    Phosphodiesterase 8A
    Hs.9333  [show with all ESTs]
    Unigene Representative Sequence: BC060762
    16 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000310298(uc002blh.3 uc002bli.3 uc010bnc.3 uc010bnd.3 uc002blj.3)
    ENST00000557957(uc021stv.1) ENST00000339708 ENST00000478717 ENST00000485596
    ENST00000559742 ENST00000559086 ENST00000560789(uc002blk.3) ENST00000557819
    ENST00000558543 ENST00000557954 ENST00000561105 ENST00000561374 ENST00000560333
    ENST00000561024(uc002bll.3) ENST00000394553
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat PDE8A using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PDE8A (see all 34):
    hsa-miR-376b hsa-miR-199a-3p hsa-miR-513a-5p hsa-miR-25 hsa-miR-488 hsa-miR-133a hsa-miR-92b hsa-miR-616
    SwitchGear 3'UTR luciferase reporter plasmidPDE8A 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for PDE8A
    Predesigned siRNA for gene silencing in human, mouse, rat PDE8A
    Clone
    Products:
         
    OriGene clones in human, mouse for PDE8A (see all 11)
    OriGene ORF clones in mouse, rat for PDE8A
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): PDE8A (NM_002605)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PDE8A
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDE8A
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for PDE8A
    OriGene qSTAR qPCR primer pairs in human, mouse for PDE8A
    Pre-validated RT2 qPCR Primer Assay in human, mouse / rat PDE8A
      QuantiTect SYBR Green Assays in human, mouse, rat PDE8A
      QuantiFast Probe-based Assays in human, mouse, rat PDE8A

    Additional mRNA sequence: 

    AF056490.1 AF332653.1 AF388183.1 AF388184.1 AK074280.1 AL109687.1 AL109778.1 BC013825.1 
    BC017164.2 BC036190.1 BC037260.1 BC048317.1 BC060762.1 BC075822.1 

    18 DOTS entries:

    DT.65286688  DT.95191818  DT.451840  DT.100788096  DT.91934162  DT.100646597  DT.100788095  DT.101961527 
    DT.40194645  DT.91719370  DT.100788094  DT.121033709  DT.40116709  DT.86840167  DT.86854293  DT.92415758 
    DT.92435159  DT.100675011 

    Selected AceView cDNA sequences (see all 218):

    AA626586 BF591694 BC037260 BC036190 BM986931 CA777537 AV652594 AA233564 
    AV725689 AK074280 BQ722402 W72944 BX281079 AK122689 AK127232 BC075822 
    AI127217 AA261827 BC060762 W60881 BM453103 W73272 AA631862 AI670875 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for PDE8A (see all 10)    About this scheme

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b ^ 19 ^ 20a · 20b ^
    SP1:              -                                                           -                                         -                 -           -         
    SP2:        -     -                                         -                 -                                         -                 -           -         
    SP3:              -                                         -                 -                                         -                 -           -         
    SP4:              -                                         -     -           -                                         -                 -           -         
    SP5:              -                                   -     -                 -                                         -                 -           -         

    ExUns: 21 ^ 22 ^ 23 ^ 24a · 24b · 24c
    SP1:                                    
    SP2:                                    
    SP3:                                    
    SP4:                                    
    SP5:                                    


    ECgene alternative splicing isoforms for PDE8A

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    PDE8A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCAATGTATT
    PDE8A Expression
    About this image


    PDE8A expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 11) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Myelinating Oligodendrocyte Cells Forebrain White Matter
             Cerebral Cortex
     
     Liver (Hepatobiliary System)    fully expand to see all 2 entries
             Hepatocytes Liver Lobule
     
     Peripheral Nervous System (Nervous System)
             Schwann Precursor Cells Peripheral Nerve Domain
     
     Bone (Muscoskeletal System)
             Membranous Facial Bones
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
    PDE8A Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    PDE8A Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.9333

    UniProtKB/Swiss-Prot: PDE8A_HUMAN, O60658
    Tissue specificity: Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in
    testis, ovary, small intestine and colon

        Custom PCR Arrays for PDE8A
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for PDE8A
    OriGene qSTAR qPCR primer pairs in human, mouse for PDE8A
    Pre-validated RT2 qPCR Primer Assay in human, mouse / rat PDE8A
    QuantiTect SYBR Green Assays in human, mouse, rat PDE8A
    QuantiFast Probe-based Assays in human, mouse, rat PDE8A
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDE8A

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for PDE8A gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Pde8a1 , 5 phosphodiesterase 8A1, 5 83.62(n)1
    81.53(a)1
      7 (45.71 cM)5
    185841  NM_008803.21  NP_032829.11 
     812135965 
    chicken
    (Gallus gallus)
    Aves PDE8A1 phosphodiesterase 8A 77.9(n)
    79.59(a)
      415455  XM_413834.4  XP_413834.2 
    lizard
    (Anolis carolinensis)
    Reptilia PDE8A6
    phosphodiesterase 8A
    80(a)
    1 ↔ 1
    GL343657.1(143623-183626)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia pde8a1 phosphodiesterase 8A 73.02(n)
    75.51(a)
      100497070  XM_002939622.2  XP_002939668.2 
    zebrafish
    (Danio rerio)
    Actinopterygii pde8a1 phosphodiesterase 8A 67.98(n)
    70.69(a)
      791145  NM_001080627.1  NP_001074096.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG54113 cAMP-specific phosphodiesterase 49(a)     --
    worm
    (Caenorhabditis elegans)
    Secernentea Y95B8A.103
    pde-61
    phosphodiesterase3
    pde-61
    31(a)3
    47.38(n)1
    38.86(a)1
      I(873483-882715)3
    1908081  NM_058386.11  NP_490787.11 


    ENSEMBL Gene Tree for PDE8A (if available)
    TreeFam Gene Tree for PDE8A (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for PDE8A gene
    PDE4D2  PDE8B2  PDE4B2  PDE4C2  PDE7B2  PDE1B2  PDE1A2  PDE9A2  
    PDE1C2  PDE7A2  PDE4A2  
    12 SIMAP similar genes for PDE8A using alignment to 5 protein entries:     PDE8A_HUMAN (see all proteins):
    PDE8B    PDE4D    PDE4C    PDE4    PDE1A    PDE1C
    PDE3A    PDE9A    DKFZp686L23131    PDE1B    PDE7B    PDE7B variant protein

    Find genes that share paralogs with PDE8A           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for PDE8A (see all 3456)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 15 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs358189841,2
    C--61633956(+) TATTC-/T/TT  
            
    TTTTT
    3 -- int11NA 2
    rs677234561,2
    C--61639982(+) CCCCA-/TGTCGT 3 -- int10--------
    rs1135434161,2
    C--61671211(+) ACCAAT/-TTTTT 3 -- int11Minor allele frequency- -:0.00CSA 2
    rs3773691,2
    C,F,A--61671223(+) TTTTTA/TAATAA 3 -- int1 trp37Minor allele frequency- T:0.45NA WA EA 366
    rs2003988781,2
    C--61671223(+) TTTTT-/A/TAATAA 3 -- int10--------
    rs113084981,2
    C,F--61695156(+) AGCAA-/T/TT  
            
    TTTTT
    3 -- int1 trp33NA CSA 6
    rs2005777251,2
    --61695165(+) TTTTT-/TAACTTG 3 -- int10--------
    rs344506651,2
    C--61702098(+) CATGGT/-TGGCA 3 -- int11Minor allele frequency- -:0.50CSA 2
    rs340050571,2
    C--61707376(+) AAAAC-/AG/GA 
            
    AAGAC
    3 -- int11NA 2
    rs340391171,2
    C--61711349(+) ACCTC-/TTGTCA 3 -- int10--------

    HapMap Linkage Disequilibrium report for PDE8A (85523671 - 85682376 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for PDE8A (see all 11):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2672879CNV Deletion23128226
    nsv1646CNV Insertion18451855
    esv273890CNV Insertion20981092
    nsv94721CNV Loss16902084
    nsv833078CNV Loss17160897
    nsv94588CNV Loss16902084
    nsv1645CNV Loss18451855
    nsv827419CNV Gain20364138
    nsv428311CNV Gain18775914
    nsv833079CNV Gain17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing PDE8A
    DNA2.0 Custom Variant and Variant Library Synthesis for PDE8A

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 602972    OMIM disorders: --

    1 disease for PDE8A:    
    About MalaCards
    granulomatous amebic encephalitis

    1 disease from the University of Copenhagen DISEASES database for PDE8A:
    Granulomatous amebic encephalitis

    Find genes that share disorders with PDE8A           About GenesLikeMe

    Genetic Association Database (GAD): PDE8A
    Human Genome Epidemiology (HuGE) Navigator: PDE8A (4 documents)

    Export disorders for PDE8A gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for PDE8A gene, integrated from 10 sources (see all 35):
    (articles sorted by number of sources associating them with PDE8A)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase. (PubMed id 9618252)1, 2, 3, 9 Fisher D.A.... Cheng J.B. (Biochem. Biophys. Res. Commun. 1998)
    2. Human phosphodiesterase 8A splice variants: cloning, gene organization, and tissue distribution. (PubMed id 11738832)1, 2, 9 Wang P.... Billah M.M. (Gene 2001)
    3. Kinetic and structural studies of phosphodiesterase-8A and implication on the inhibitor selectivity. (PubMed id 18983167)1, 2, 9 Wang H.... Ke H. (Biochemistry 2008)
    4. PDE8A genetic variation, polycystic ovary syndrome and androgen levels in women. (PubMed id 19482904)1, 4, 9 Chen C....Strauss J.F. (Mol. Hum. Reprod. 2009)
    5. Cyclic AMP-specific phosphodiesterase, PDE8A1, is activated by protein kinase A-mediated phosphorylation. (PubMed id 22673573)1, 2 Brown K.M.... Baillie G.S. (FEBS Lett. 2012)
    6. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (Diabetes Care 2010)
    7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    9. T cell activation up-regulates cyclic nucleotide phosphodiesterases 8A1 and 7A3. (PubMed id 11371644)1, 2 Glavas N.A.... Beavo J.A. (Proc. Natl. Acad. Sci. U.S.A. 2001)
    10. Genetic variants associated with disordered eating. (PubMed id 23568457)1 Wade T.D....Martin N.G. (Int J Eat Disord 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 5151 HGNC: 8793 AceView: PDE8A Ensembl:ENSG00000073417 euGenes: HUgn5151
    ECgene: PDE8A Kegg: 5151 H-InvDB: PDE8A

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for PDE8A Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for PDE8A gene:
    Search GeneIP for patents involving PDE8A

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for PDE8A   OriGene RNAi products in human, mouse, rat for PDE8A  
     OriGene qPCR primer pairs and template standards for PDE8A   OriGene Protein Over-expression Lysate for PDE8A  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for PDE8A  
     OriGene qSTAR qPCR primer pairs in human, mouse for PDE8A   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for PDE8A   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for PDE8A   OriGene Custom Protein Services for PDE8A  

     
     
     Block miRNA regulation of human, mouse, rat PDE8A using miScript Target Protectors SeqTarget long-range PCR primers for resequencing PDE8A
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PDE8A Predesigned siRNA for gene silencing in human, mouse, rat PDE8A
     QuantiFast Probe-based Assays in human, mouse, rat PDE8A QuantiTect SYBR Green Assays in human, mouse, rat PDE8A
     Custom PCR Arrays for PDE8A Search Chromatin IP Primers for PDE8A
     Pre-validated RT2 qPCR Primer Assay in human, mouse / rat PDE8A  Search GeneGlobe Interaction Network for PDE8A
     Regulatory tfbs in PDE8A promoter
     GenScript Custom Purified and Recombinant Proteins Services for PDE8A GenScript cDNA clones with any tag delivered in your preferred vector for PDE8A
     GenScript Custom Assay Services for PDE8A GenScript Custom overexpressing Cell Line Services for PDE8A
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Tocris compounds for PDE8A
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     PDE8A antibodies
     PDE8A proteins
     PDE8A lysates
     Antibodies for PDE8A
     See all of Abcam's Antibodies, Kits and Proteins for PDE8A
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for PDE8A
     Antibodies for PDE8A
     ELISAs for PDE8A
     CLIAs for PDE8A



     Browse ESI BIO Cell Lines and PureStem Progenitors for PDE8A
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDE8A
     SwitchGear 3'UTR luciferase reporter plasmids for PDE8A
     SwitchGear Promoter luciferase reporter plasmids for PDE8A
     ThermoFisher Antibodies for PDE8A
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDE8A
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PDE8A
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for PDE8A
     LSBio Antibodies in human, mouse, rat for PDE8A
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Search Addgene for plasmids for PDE8A
      Search eBioscience for proteins for PDE8A
      Search eBioscience for elisas for PDE8A
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      PDE8A gene at Home site.
    Version: 3.12.212 17 Sep 2014
    hostname: 356980-web2.xennexinc.com index build: 128 solr: 1.4