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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

PDE3A Gene

protein-coding   GIFtS: 65
GCID: GC12P020422

phosphodiesterase 3A, cGMP-inhibited
 Explore 17 diseases affiliated with
PDE3A via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for PDE3A    

Aliases
for PDE3A gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
Phosphodiesterase 3A, CGMP-Inhibited1 2     CGI-PDE-A2
CGI-PDE1 2     CAMP Phosphodiesterase, Myocardial CGMP-Inhibited2
Cyclic GMP-Inhibited Phosphodiesterase A2 3     CGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A2
CGI-PDE A2 3     EC 3.1.48
EC 3.1.4.173 8     

External Ids:    HGNC: 87781   Entrez Gene: 51392   Ensembl: ENSG000001725727   OMIM: 1238055   UniProtKB: Q144323   

Export aliases for PDE3A gene to outside databases

Previous GC identifers: GC12P020791 GC12P020523 GC12P020413 GC12P020294


Summaries
for PDE3A gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for PDE3A:
This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes
hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and
duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays
important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors
of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript
variants encoding multiple isoforms have been observed for this gene. (provided by RefSeq, Sep 2011)

UniProtKB/Swiss-Prot: PDE3A_HUMAN, Q14432
Function: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which
are key regulators of many important physiological processes (By similarity)

summary for PDE3A:
Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the
hydrolysis of 3' cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or
cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by
controlling the rate of their degradation. There are 11 subtypes of PDEs, named PDE1-11; PDE4, 7 and 8
selectively degrade cAMP, PDE5, 6 and 9 selectively degrade cGMP and PDE1, 2, 3, 10 and 11 degrade both
cyclic nucleotides. PDEs are expressed ubiquitously, with each subtype having a specific tissue
distribution. These enzymes are involved in many signal transduction pathways and their functions include
vascular smooth muscle proliferation and contraction, cardiac contractility, platelet aggregation, hormone
secretion, immune cell activation, and they are involved in learning and memory.




Genomic Views
for PDE3A gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_009714.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the PDE3A gene promoter:
         STAT5B   Pax-5   CUTL1   Evi-1   POU2F1   POU2F1a   POU2F1b   POU2F1c   AP-2alpha   RSRFC4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: PDE3A promoter sequence
   Search SABiosciences Chromatin IP Primers for PDE3A

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PDE3A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12p12   Ensembl cytogenetic band:  12p12.2   HGNC cytogenetic band: 12p12.2

PDE3A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PDE3A gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P020422:  view genomic region     (about GC identifiers)

Start:
 20,522,179 bp from pter      End:
 20,837,315 bp from pter
Size:
 315,137 bases      Orientation:
 plus strand

Proteins
for PDE3A gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: PDE3A_HUMAN, Q14432 (See protein sequence)
Recommended Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A  
Size: 1141 amino acids; 124979 Da
Cofactor: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a
preference for magnesium and/or manganese ions (By similarity)
Subcellular location: Membrane; Multi-pass membrane protein (Potential)
1 PDB 3D structure from and Proteopedia for PDE3A:
1LRC (3D)    
Secondary accessions: O60865 Q13348 Q17RD1

Explore the universe of human proteins at neXtProt for PDE3A: NX_Q14432

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q14432

    • 4/6 DME Specific Peptides for PDE3A (Q14432) (see all 6)
     EFYEQGD  SVLENHH  LDHVEFK  TSATGLPT 

    PDE3A Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_000912.3  NP_001231612.1  

    ENSEMBL proteins: 
     ENSP00000351957  
    Reactome Protein details: Q14432
    Human Recombinant Protein Products: 
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    OriGene Protein Over-expression Lysate: PDE3A
    OriGene Custom Protein Services for PDE3A 
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    Novus Biologicals PDE3A Protein
    Novus Biologicals PDE3A Lysate
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for PDE3A

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005625soluble fraction ----
    GO:0005626insoluble fraction ----
    GO:0005829cytosol TAS--
    GO:0016021integral to membrane IEA--


    PDE3A for ontologies           About GeneDecksing



    PDE3A Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of PDE3A
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    Novus Biologicals PDE3A Antibodies
    Search for Antibodies for PDE3A at Abcam  
    Uscn Antibodies for PDE3A
    ThermoFisher Antibodies for PDE3A

    Assay Products for PDE3A: 
    Browse Kits and Assays available from EMD Millipore
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    Uscn ELISAs and CLIAs for PDE3A

    Protein Domains /
    Families
    for PDE3A gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    PDE3A for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR002073 PDEase_catalytic_dom
     IPR023174 PDEase_CS
     IPR003607 HD/PDEase_dom

    Graphical View of Domain Structure for InterPro Entry Q14432

    ProtoNet protein and cluster: Q14432

    1 Blocks protein family: IPB001917 Aminotransferase

    UniProtKB/Swiss-Prot: PDE3A_HUMAN, Q14432
    Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily


    Function
    for PDE3A gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: PDE3A_HUMAN, Q14432
    Function: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which
    are key regulators of many important physiological processes (By similarity)
    Catalytic activity: Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
    Enzyme regulation: Inhibited by cGMP

         Genatlas biochemistry entry for PDE3A:
    cAMP,cGMP inhibited nucleotide phosphodiesterase 3A homolog,involved in fat metabolism

    Enzyme Numbers (IUBMB): EC 3.1.4.171 2 EC 3.1.42

    miRNA
    Products:            		miRTarBase miRNAs that target PDE3A:
    hsa-mir-155 (MIRT001528)

    OriGene 3'-UTR Clone: PDE3A
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat PDE3A
    8/16 QIAGEN miScript miRNA Assays for microRNAs that regulate PDE3A (see all 16):
    hsa-miR-362-3p hsa-miR-3607-3p hsa-miR-27a hsa-miR-128 hsa-miR-139-5p hsa-miR-3681* hsa-miR-218 hsa-miR-329
    SwitchGear 3'UTR luciferase reporter plasmid: PDE3A 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PDE3A (see all 7)
    OriGene shRNA RFP: PDE3A
    OriGene siRNA: PDE3A
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PDE3A

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for PDE3A

    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PDE3A (see all 3)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PDE3A
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: PDE3A (NM_000921)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PDE3A
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PDE3A 

    Cell Line
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    Search LifeMap BioReagents cell lines for PDE3A

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PDE3A

    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:00041153',5'-cyclic-AMP phosphodiesterase activity IEA--
    GO:0004119cGMP-inhibited cyclic-nucleotide phosphodiesterase activity IEA--
    GO:0030552cAMP binding IEA--
    GO:0046872metal ion binding IEA--


    PDE3A for ontologies           About GeneDecksing


    4 GenomeRNAi human phenotypes for PDE3A:
     Increased homologous recombina  Increased viability after born  Synthetic lethal with Ras  Synthetic lethal with paclitax 

    Animal Models:
         Mouse knock-out Pde3atm1Vman for PDE3A
         2 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Pde3a):
     normal  reproductive system 

    PDE3A for phenotypes           About GeneDecksing


    Pathways & Interactions
    for PDE3A gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/14 super-pathways (see all 14) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Sweet Taste Signaling
    		Sweet Taste Signaling1.00
    		Bitter Taste Signaling0.44
    		Cellular Effects of Sildenafil0.46
    		Sperm Motility0.36
    2Activation of cAMP-Dependent PKA
    		Activation of cAMP-Dependent PKA1.00
    		Activation of PKA through GPCR0.71
    		cAMP Pathway0.77
    		PKA Signaling0.56
    3Nitric oxide stimulates guanylate cyclase
    		Nitric oxide stimulates guanylate cyclase1.00
    		Platelet homeostasis0.34
    		cGMP effects0.75
    4Signaling by GPCR
    		Signaling by GPCR1.00
    		Signal Transduction0.56
    		GPCR downstream signaling0.89
    5PKA activation in glucagon signalling
    		Development Beta-adrenergic receptors signaling via cAMP0.29
    		Development_Beta-adrenergic receptors signaling via cAMP0.29

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    2 EMD Millipore Pathways for PDE3A
        Development Beta-adrenergic receptors signaling via cAMP
    Signal transduction PKA signaling

    5/10 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for PDE3A (see all 10)
        Activation of PKA through GPCR
    PKA Signaling
    Sweet Taste Signaling
    Relaxin Pathway
    Activation of PKC through GPCR

    2 GeneGo (Thomson Reuters) Pathways for PDE3A
        Development Beta-adrenergic receptors signaling via cAMP
    Signal transduction PKA signaling

    5/8        Reactome Pathways for PDE3A (see all 8)
        GPCR downstream signaling
    Hemostasis
    cGMP effects
    Signal Transduction
    Platelet homeostasis

    1 PharmGKB Pathway for PDE3A
        Proton Pump Inhibitor Pathway, Pharmacodynamics

    3         Kegg Pathways  (Kegg details for PDE3A):
        Purine metabolism
    Insulin signaling pathway
    Progesterone-mediated oocyte maturation


    PDE3A for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for PDE3A

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/62 Interacting proteins for PDE3A (Q144322, 3 ENSP000003519574) via UniProtKB, MINT, STRING, and/or I2D (see all 62)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    YWHAZP631042, 3MINT-3295899 I2D: score=1 
    YWHAQP273483, ENSP000002380814I2D: score=1 STRING: ENSP00000238081
    ADCY10ENSP000003568254STRING: ENSP00000356825
    ADCY2ENSP000003429524STRING: ENSP00000342952
    ADCY3ENSP000002606004STRING: ENSP00000260600
    About this table

    Gene Ontology (GO): 5/16 biological process terms (GO ID links to tree view) (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001556oocyte maturation IEA--
    GO:0006198cAMP catabolic process IMP19252089
    GO:0006629lipid metabolic process TAS8921398
    GO:0007596blood coagulation TAS--
    GO:0016101diterpenoid metabolic process IEA--


    PDE3A for ontologies           About GeneDecksing



    Drugs & Compounds
    for PDE3A gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    PDE3A for compounds           About GeneDecksing

    EMD Millipore small molecules for PDE3A:
    Small Molecule - inhibitor
    Browse drugs & compounds from Enzo Life Sciences

    Compounds for PDE3A available from Tocris Bioscience    About this table
    CompoundAction CAS #
    MilrinonePDE3 inhibitor[78415-72-2]
    CilostazolPDE3A inhibitor. Also adenosine uptake inhibitor[73963-72-1]
    CilostamidePDE3 inhibitor[68550-75-4]
    ZardaverinePDE3/4 inhibitor[101975-10-4]
    Anagrelide hydrochloridePotent PDE3 inhibitor[58579-51-4]

    6 HMDB Compounds for PDE3A    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine monophosphate5'-AMP (see all 28)61-19-8--
    Cyclic AMPCyclic AMP (see all 19)60-92-4--
    Cyclic GMP3',5'-cyclic GMP (see all 13)7665-99-8--
    Guanosine monophosphate5'-GMP (see all 14)85-32-5--
    Theophylline1,3-Dimethylxanthine (see all 96)58-55-9--
    WaterDihydrogen oxide (see all 2)7732-18-5--

    10/11 DrugBank Compounds for PDE3A (see all 11)    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Milrinone-- 78415-72-2targetinhibitor10511123 12181427 10859452 12652111 12135876 11752352
    CilostazolCilostazole (see all 2)73963-72-1targetinhibitor12180353 19218671 16472148 11752352 18282775
    AmrinoneAmrinona [inn-spanish] (see all 4)60719-84-8targetinhibitor12076859 19901885 18468627 11752352
    AnagrelideAnagrelide HCL (see all 3)68475-42-3targetinhibitor17139284 11752352 17016423 2846839
    Oxtriphylline-- 4499-40-5targetinhibitor15237094 12887610 16651698
    TheophyllineDimethylxanthine (see all 6)58-55-9targetinhibitor15237094 12887610 16651698
    AminophyllineAminophyllin (see all 4)317-34-0targetinhibitor11939921 11752352
    Enoximone-- 77671-31-9targetinhibitor16987374 17714066
    Ibudilast2-Isopropyl-3-isobutyrylpyrazolo(1,5-a)pyridine (see all 18)50847-11-5targetinhibitor21036126
    Levosimendan-- 141505-33-1targetinhibitor16116344

    10/14 Novoseek chemical compound relationships for PDE3A gene (see all 14)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    8-((4-bromo-2,3-dioxobutyl)thio)-adenosine 3',5'-cyclic monophosphate 93.1 2 11955000 (1), 11863434 (1)
    cilostamide 91.5 8 8395509 (1), 8812137 (1), 19332778 (1), 1313303 (1) (see all 6)
    milrinone 85.4 11 7860763 (2), 1324870 (2), 8812137 (1), 1652182 (1) (see all 7)
    cgmp 77 55 11468344 (4), 8713077 (3), 17704206 (3), 18394675 (3) (see all 18)
    cilostazol 67.2 5 15354266 (1), 14645013 (1), 20136824 (1), 10644042 (1)
    amrinone 64.7 3 1324870 (2), 10644042 (1)
    forskolin 55.5 5 9031467 (2), 11832336 (1), 16153182 (1)
    cyclic amp 47.8 1 17392505 (5), 18467075 (1)
    isoproterenol 41.3 9 15867171 (3), 11832336 (1), 16186489 (1)
    adenylate 31.4 2 15354266 (1), 9031467 (1), 17392505 (1)

    Search CenterWatch for drugs/clinical trials and news about PDE3A 

    Transcripts
    for PDE3A gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for PDE3A gene (2 alternative transcripts): 
    NM_000921.4  NM_001244683.1  

    Unigene Clusters for PDE3A:

    Phosphodiesterase 3A, cGMP-inhibited
    Hs.386791  [show with all ESTs], Hs.737522
    Unigene Representative Sequences: NM_000921, AK095203
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000359062(uc001reh.2 uc021qwa.1) ENST00000542675 ENST00000544307


    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat PDE3A
    8/16 QIAGEN miScript miRNA Assays for microRNAs that regulate PDE3A (see all 16):
    hsa-miR-362-3p hsa-miR-3607-3p hsa-miR-27a hsa-miR-128 hsa-miR-139-5p hsa-miR-3681* hsa-miR-218 hsa-miR-329
    SwitchGear 3'UTR luciferase reporter plasmid: PDE3A 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PDE3A (see all 7)
    OriGene shRNA RFP: PDE3A
    OriGene siRNA: PDE3A
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PDE3A
    Clone
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    GenScript: all cDNA clones in your preferred vector: PDE3A (NM_000921)
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    Primer
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    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat PDE3A
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PDE3A
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PDE3A

    Additional cDNA sequence: AK095203.1 

    2 DOTS entries:

    DT.109816  DT.121120039 

    24/84 AceView cDNA sequences (see all 84):

    AI628567 AI961922 CK300107 BF593925 BM726612 BQ187047 BF435350 BU632064 
    AI597722 BX501734 BQ005307 AA574127 AL600188 AI264257 AL600910 AW072688 
    AA313509 AW008123 BQ005500 AI687304 AI683378 AI624266 CA430169 CB985950 

    GeneLoc Exon Structure


    Expression
    for PDE3A gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    PDE3A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGTGTGCATA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    PDE3A expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    6 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    Gut TubeMidgutMidgut Endoderm CellsEndoderm
    BoneEndochondral Facial BonesBone
    Gut TubeMidgutGut Tube
    HeartDorsal AortaHeart
    KidneyInterstitial StromaKidney
    Neural TubeTelencephalonNeural Tube
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Pancreatic endoderm/endocrine precursor-like cells (A scalable, suspensi...)

    See PDE3A Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for PDE3A

    SOURCE GeneReport for Unigene clusters: Hs.386791 Hs.737522
        SABiosciences Expression via Pathway-Focused PCR Array including PDE3A: 
              Hypertension in human mouse rat

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    Orthologs
    for PDE3A gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of animals.

    Orthologs for PDE3A gene from 5/20 species (see all 20)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    chicken
    (Gallus gallus)    		 Aves PDE3A1 phosphodiesterase 3A, cGMP-inhibited 76.84(n)
    82.72(a)    		   418185  XM_416416.3  XP_416416.3 
    lizard
    (Anolis carolinensis)    		 Reptilia PDE3A6
    		 --
    		 73(a)
    		 1 ↔ 1
    		 5(8490042-8524432)
    African clawed frog
    (Xenopus laevis)    		 Amphibia BX851299.12   -- 76.02(n)    BX851299.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii Dr.111812 Transcribed sequence with weak similarity to protein more 75.74(n)    BG307206.1 
    worm
    (Caenorhabditis elegans)    		 Secernentea pde-31 Protein PDE-3 51.12(n)
    46.63(a)    		   183981  NM_182143.2  NP_871943.2 


    ENSEMBL Gene Tree for PDE3A (if available)
    TreeFam Gene Tree for PDE3A (if available) 

    Paralogs
    for PDE3A gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for PDE3A gene
    PDE4D2  PDE8B2  PDE4B2  PDE4C2  PDE1B2  PDE1A2  PDE7B2  PDE9A2  
    PDE1C2  PDE7A2  PDE8A2  PDE4A2  PDE3B2  
    3 SIMAP similar genes for PDE3A using alignment to 1 protein entry:     PDE3A_HUMAN:
    PDE4    PDE3B    PDE8A

    PDE3A for paralogs           About GeneDecksing



    Genomic Variants
    for PDE3A gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/5649 NCBI SNPs in PDE3A are shown (see all 5649)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 12 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs794873101,2
    		C,--20292166(+) AAGTGG/TAGTAT 1 -- us2k13Minor allele frequency- T:0.07NA EA 242
    rs796386711,2
    		--20293974(+) GGGTGG/CAATTG 1 -- us2k12Minor allele frequency- C:0.02CSA WA 119
    rs10422621,2
    		H--20294282(+) TCCTCC/TGCCGC 2 S syn11Minor allele frequency- T:0.00NS 108
    rs1124391851,2
    		C,--20295601(+) TTCTGG/ATTTTG 1 -- int11Minor allele frequency- A:0.50NA 2
    rs615640901,2
    		C,--20295821(+) ACTTAC/AGTGGA 1 -- int11Minor allele frequency- A:0.50WA 2
    rs580321251,2
    		C,--20295850(+) GATTTA/GTGCAG 1 -- int12Minor allele frequency- G:0.04WA 120
    rs796116921,2
    		--20296493(+) TGTTTC/TGATAA 1 -- int11Minor allele frequency- T:0.01EA 120
    rs774411321,2
    		--20297408(+) TATCTG/ATTTTA 1 -- int11Minor allele frequency- A:0.01EA 120
    rs745661111,2
    		F,--20297858(+) TTTTTT/CTCTTT 1 -- int11Minor allele frequency- C:0.11WA 118
    rs776157401,2
    		F,--20298977(+) ACAAGA/TTTTCC 1 -- int11Minor allele frequency- T:0.09NA 120

    HapMap Linkage Disequilibrium report for PDE3A (20522179 - 20772179 bp, first 250kb of PDE3A)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 7 variations for PDE3A
         2 CNVs: 86251 75970
         5 Indels: 86254 75971 86253 60081 86252

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    Disorders / Diseases
    for PDE3A gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    PDE3A for disorders           About GeneDecksing

    OMIM gene information: 123805    OMIM disorders: --

    17 diseases for PDE3A:    About MalaCards
    congestive heart failure    sickle cell disease    dilated cardiomyopathy    brachydactyly
    cystic fibrosis    squamous cell carcinoma    pulmonary hypertension    azoospermia
    cardiomyopathy    thrombosis    fibrosis    hypertension
    osteosarcoma    melanoma    carcinoma    malaria
    neuronitis

    3 Novoseek disease relationships for PDE3A gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    erythroleukemia 33 2 8695850 (1), 9031467 (1)
    erectile dysfunction 15.5 3 10421499 (2), 10859452 (1)
    heart failure 4.43 3 15867171 (2), 17332439 (1)

    Human Genome Epidemiology (HuGE) Navigator: PDE3A (5 documents)

    Export disorders for PDE3A gene to outside databases

    Publications
    for PDE3A gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for PDE3A gene, integrated from 9 sources (see all 129):
    (articles sorted by number of sources associating them with PDE3A)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase. (PubMed id 1315035)1, 2, 3, 9 Meacci E.... Manganiello V. (1992)
    2. Single-step affinity purification, partial structure and properties of human platelet cGMP inhibited cAMP phosphodiesterase. (PubMed id 8155697)1, 2, 9 Degerman E.... Manganiello V. (1994)
    3. Molecular biological characterization of phosphodiesterase 3A from human corpus cavernosum. (PubMed id 10421499)1, 2, 9 Kuthe A....Maegert H.-J. (1999)
    4. Human platelet cGI-PDE: expression in yeast and localization of the catalytic domain by deletion mutagenesis. (PubMed id 8695850)1, 2, 9 Cheung P.P....Colman R.W. (1996)
    5. Cardiac type cGMP-inhibited phosphodiesterase (PDE3A) gene structure: similarity and difference to adipocyte type PDE3B gene. (PubMed id 10679291)1, 3, 9 Kasuya J....Fujita-Yamaguchi Y. (2000)
    6. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)1, 2 Beausoleil S.A.... Gygi S.P. (2006)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    8. Protein kinase C-mediated phosphorylation and activation of PDE3A regulate cAMP levels in human platelets. (PubMed id 19261611)1, 9 Hunter R.W....Hers I. (2009)
    9. Phosphodiesterase 3A binds to 14-3-3 proteins in response to PMA-induced phosphorylation of Ser428. (PubMed id 16153182)1, 9 Pozuelo Rubio M....Mackintosh C. (2005)
    10. Thrombin regulates intracellular cyclic AMP concentration in human platelets through phosphorylation/activation of phosphodiesterase 3A. (PubMed id 17392505)1, 9 Zhang W. and Colman R.W. (2007)

    External Searches for PDE3A gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing PDE3A gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 5139 HGNC: 8778 AceView: PDE3A Ensembl:ENSG00000172572 euGenes: HUgn5139
    ECgene: PDE3A Kegg: 5139 H-InvDB: PDE3A

    Other Databases showing PDE3A gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing PDE3A gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for PDE3A Pharmacogenomics, SNPs, Pathways
    Wikipedia http://en.wikipedia.org/wiki/PDE3

    Intellectual Property
    for PDE3A gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for PDE3A gene:
    Search GeneIP for patents involving PDE3A

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    About This Section

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