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Aliases for PCNA Gene

Aliases for PCNA Gene

  • Proliferating Cell Nuclear Antigen 2 3
  • Cyclin 3 4
  • DNA Polymerase Delta Auxiliary Protein 3
  • ATLD2 3

External Ids for PCNA Gene

Summaries for PCNA Gene

Entrez Gene Summary for PCNA Gene

  • The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

GeneCards Summary for PCNA Gene

PCNA (Proliferating Cell Nuclear Antigen) is a Protein Coding gene. Diseases associated with PCNA include large cell acanthoma and anthracosilicosis. Among its related pathways are Cell Cycle, Mitotic and Cell Cycle, Mitotic. GO annotations related to this gene include identical protein binding and MutLalpha complex binding.

UniProtKB/Swiss-Prot for PCNA Gene

  • Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerases processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3-5 exonuclease and 3-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while Lys-63-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Gene Wiki entry for PCNA Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PCNA Gene

Genomics for PCNA Gene

Genomic Location for PCNA Gene

Start:
5,114,953 bp from pter
End:
5,126,626 bp from pter
Size:
11,674 bases
Orientation:
Minus strand

Genomic View for PCNA Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PCNA Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PCNA Gene

Regulatory Elements for PCNA Gene

Proteins for PCNA Gene

  • Protein details for PCNA Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P12004-PCNA_HUMAN
    Recommended name:
    Proliferating cell nuclear antigen
    Protein Accession:
    P12004
    Secondary Accessions:
    • B2R897
    • D3DW02

    Protein attributes for PCNA Gene

    Size:
    261 amino acids
    Molecular mass:
    28769 Da
    Quaternary structure:
    • Homotrimer (PubMed:24939902). Interacts with p300/EP300; the interaction occurs on chromatin in UV-irradiated damaged cells (PubMed:24939902). Interacts with CREBBP (via transactivation domain and C-terminus); the interaction occurs on chromatin in UV-irradiated damaged cells (PubMed:24939902). Interacts with POLD1 (PubMed:24939902). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with PARPBP. Interacts (when ubiquitinated) with SPRTN; leading to enhance RAD18-mediated PCNA ubiquitination. Interacts (when polyubiquitinated) with ZRANB3. Interacts with SMARCAD1. Interacts with CDKN1C. Interacts with KIAA0101/PAF15 (via PIP-box). Interacts with RTEL1 (via PIP-box); the interaction is direct and essential for the suppression of telomere fragility. Interacts with FAM111A (via PIP-box); the interaction is direct and required for PCNA loading on chromatin binding. Interacts with LIG1. Interacts with SETMAR. Interacts with ANKRD17.
    Miscellaneous:
    • Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus

    Three dimensional structures from OCA and Proteopedia for PCNA Gene

neXtProt entry for PCNA Gene

Proteomics data for PCNA Gene at MOPED

Post-translational modifications for PCNA Gene

  • Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation (PubMed:24939902, PubMed:19419956). Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner (PubMed:24939902). Acetylation disrupts interaction with NUDT15 and promotes degradation (PubMed:19419956).
  • Methylated on glutamate residues by ARMT1/C6orf211.
  • Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.
  • Ubiquitinated (PubMed:24939902). Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through Lys-63 linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase.
  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys13, Lys14, Lys77, Lys80, Lys110, Lys117, Lys138, Lys164, Lys181, Lys248, and Lys254

Other Protein References for PCNA Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for PCNA Gene

Domains for PCNA Gene

UniProtKB/Swiss-Prot:

PCNA_HUMAN
Family:
  • Belongs to the PCNA family.:
    • P12004
genes like me logo Genes that share domains with PCNA: view

No data available for Gene Families for PCNA Gene

Function for PCNA Gene

Molecular function for PCNA Gene

GENATLAS Biochemistry: cell-cycle regulated nuclear protein,proliferating cell nuclear antigen,complexing with RFCs to form a processivity factor (sliding clamp),for DNA polymerases delta and epsilon,also complexing with RFC,FEN1,GADD45,MSH2,MLH1,CDKN1A,LIG1,also involved in nucleotide mismatch repair (MM) and nucleotide excision repair (NER)
UniProtKB/Swiss-Prot Function: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerases processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3-5 exonuclease and 3-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while Lys-63-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Gene Ontology (GO) - Molecular Function for PCNA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity IDA 11005803
GO:0003677 DNA binding --
GO:0003682 chromatin binding IDA 24939902
GO:0003684 damaged DNA binding IDA 24939902
GO:0005515 protein binding IPI 10873631
genes like me logo Genes that share ontologies with PCNA: view
genes like me logo Genes that share phenotypes with PCNA: view

Animal Models for PCNA Gene

MGI Knock Outs for PCNA:

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for PCNA Gene

Localization for PCNA Gene

Subcellular locations from UniProtKB/Swiss-Prot for PCNA Gene

Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. {ECO:0000269 PubMed:24939902}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PCNA Gene COMPARTMENTS Subcellular localization image for PCNA gene
Compartment Confidence
nucleus 5
cytoskeleton 4
extracellular 3
cytosol 2
mitochondrion 2
plasma membrane 2
endoplasmic reticulum 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for PCNA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 15494374
GO:0005654 nucleoplasm TAS --
GO:0005663 DNA replication factor C complex TAS 2565339
GO:0005737 cytoplasm IDA --
GO:0005813 centrosome IDA 18331714
genes like me logo Genes that share ontologies with PCNA: view

Pathways for PCNA Gene

SuperPathways for PCNA Gene

genes like me logo Genes that share pathways with PCNA: view

Gene Ontology (GO) - Biological Process for PCNA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000077 DNA damage checkpoint --
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS --
GO:0000722 telomere maintenance via recombination TAS --
genes like me logo Genes that share ontologies with PCNA: view

Compounds for PCNA Gene

(133) Novoseek inferred chemical compound relationships for PCNA Gene

Compound -log(P) Hits PubMed IDs
paraffin 76.1 120
bromodeoxyuridine 75.5 200
dutp 66.5 32
thymidine 61.3 113
vegf 59.2 182
genes like me logo Genes that share compounds with PCNA: view

Transcripts for PCNA Gene

mRNA/cDNA for PCNA Gene

Unigene Clusters for PCNA Gene

Proliferating cell nuclear antigen:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for PCNA Gene

ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8
SP1: -
SP2: - - - -
SP3: -
SP4:
SP5: -

Relevant External Links for PCNA Gene

GeneLoc Exon Structure for
PCNA
ECgene alternative splicing isoforms for
PCNA

Expression for PCNA Gene

mRNA expression in normal human tissues for PCNA Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for PCNA Gene

SOURCE GeneReport for Unigene cluster for PCNA Gene Hs.147433

genes like me logo Genes that share expressions with PCNA: view

Orthologs for PCNA Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for PCNA Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PCNA 36
  • 99.11 (n)
  • 100 (a)
PCNA 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia PCNA 36
  • 92.46 (n)
  • 99.23 (a)
PCNA 37
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PCNA 36
  • 92.21 (n)
  • 99.62 (a)
PCNA 37
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Pcna 36
  • 88.38 (n)
  • 96.93 (a)
Pcna 16
Pcna 37
  • 97 (a)
OneToMany
Pcna-ps2 37
  • 96 (a)
OneToMany
oppossum
(Monodelphis domestica)
Mammalia PCNA 37
  • 97 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PCNA 37
  • 35 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Pcna 36
  • 89.02 (n)
  • 98.47 (a)
chicken
(Gallus gallus)
Aves PCNA 36
  • 83.14 (n)
  • 94.64 (a)
PCNA 37
  • 94 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PCNA 37
  • 91 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia pcna-A 36
tropical clawed frog
(Silurana tropicalis)
Amphibia pcna 36
  • 79.69 (n)
  • 90.42 (a)
Str.6228 36
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.10814 36
zebrafish
(Danio rerio)
Actinopterygii pcna 36
  • 77.13 (n)
  • 91.86 (a)
pcna 37
  • 92 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010220 36
  • 63.85 (n)
  • 70 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG10262 37
  • 49 (a)
OneToMany
mus209 36
  • 65.89 (n)
  • 71.04 (a)
mus209 37
  • 71 (a)
OneToMany
CG10262 38
  • 47 (a)
mus209 38
  • 71 (a)
worm
(Caenorhabditis elegans)
Secernentea pcn-1 36
  • 54.62 (n)
  • 48.08 (a)
pcn-1 37
  • 48 (a)
OneToOne
pcn-1 38
  • 48 (a)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL309W 36
  • 50.06 (n)
  • 39.77 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes POL30 36
  • 47.29 (n)
  • 35.66 (a)
POL30 37
  • 35 (a)
OneToOne
POL30 39
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B12078g 36
  • 46.25 (n)
  • 36.05 (a)
soybean
(Glycine max)
eudicotyledons Gma.431 36
thale cress
(Arabidopsis thaliana)
eudicotyledons PCNA2 36
  • 64.86 (n)
  • 66.02 (a)
barley
(Hordeum vulgare)
Liliopsida Hv.6407 36
corn
(Zea mays)
Liliopsida Zm.531 36
rice
(Oryza sativa)
Liliopsida Os.3376 36
Os02g0805200 36
  • 64.09 (n)
  • 62.16 (a)
wheat
(Triticum aestivum)
Liliopsida Ta.2876 36
bread mold
(Neurospora crassa)
Ascomycetes NCU09239 36
  • 56.11 (n)
  • 54.83 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes pcn1 36
  • 53.23 (n)
  • 52.33 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.3511 36
Species with no ortholog for PCNA:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for PCNA Gene

ENSEMBL:
Gene Tree for PCNA (if available)
TreeFam:
Gene Tree for PCNA (if available)

Paralogs for PCNA Gene

Pseudogenes.org Pseudogenes for PCNA Gene

genes like me logo Genes that share paralogs with PCNA: view

No data available for Paralogs for PCNA Gene

Variants for PCNA Gene

Sequence variations from dbSNP and Humsavar for PCNA Gene

SNP ID Clin Chr 20 pos Sequence Context AA Info Type MAF
rs3626 -- 5,115,125(-) TCTCT(C/G)CAATT utr-variant-3-prime
rs8632 -- 5,115,134(-) TTTCT(C/T)TATTC utr-variant-3-prime
rs17350 -- 5,117,557(-) AAAGA(C/T)GGAGT reference, synonymous-codon
rs17351 -- 5,117,419(-) AATAC(C/T)GCTTA intron-variant
rs17352 -- 5,117,330(-) TGCGA(A/C)TGATT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PCNA Gene

Variant ID Type Subtype PubMed ID
nsv912629 CNV Gain 21882294
nsv833902 CNV Loss 17160897
nsv428375 CNV Loss 18775914

Relevant External Links for PCNA Gene

HapMap Linkage Disequilibrium report
PCNA
Human Gene Mutation Database (HGMD)
PCNA

Disorders for PCNA Gene

UniProtKB/Swiss-Prot

PCNA_HUMAN
  • Ataxia-telangiectasia-like disorder 2 (ATLD2) [MIM:615919]: A neurodegenerative disorder due to defects in DNA excision repair. ATLD2 is characterized by developmental delay, ataxia, sensorineural hearing loss, short stature, cutaneous and ocular telangiectasia, and photosensitivity. {ECO:0000269 PubMed:24911150}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(98) Novoseek inferred disease relationships for PCNA Gene

Disease -log(P) Hits PubMed IDs
tumors 78.5 310
carcinoma 72.8 170
carcinoma squamous cell 69.5 146
metastasis 66.7 180
laryngeal squamous cell carcinoma 65.6 27

Relevant External Links for PCNA

Genetic Association Database (GAD)
PCNA
Human Genome Epidemiology (HuGE) Navigator
PCNA
Tumor Gene Database (TGDB):
PCNA
genes like me logo Genes that share disorders with PCNA: view

Publications for PCNA Gene

  1. The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21. (PMID: 9305916) Gary R. … Park M.S. (J. Biol. Chem. 1997) 3 4 23
  2. Tyrosine phosphorylation controls PCNA function through protein stability. (PMID: 17115032) Wang S.C. … Hung M.C. (Nat. Cell Biol. 2006) 3 4 23
  3. Association of polymorphisms in base excision repair genes with the risk of breast cancer: a case-control study in North Indian women. (PMID: 18669164) Mitra A.K. … Rath S.K. (Oncol. Res. 2008) 3 23 49
  4. Proliferating cell nuclear antigen is protected from degradation by forming a complex with MutT Homolog2. (PMID: 19419956) Yu Y. … Zhu W.-G. (J. Biol. Chem. 2009) 3 4 23
  5. Role of PCNA-dependent stimulation of 3'-phosphodiesterase and 3'-5' exonuclease activities of human Ape2 in repair of oxidative DNA damage. (PMID: 19443450) Burkovics P. … Haracska L. (Nucleic Acids Res. 2009) 3 4 23

Products for PCNA Gene

Sources for PCNA Gene

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