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Aliases for PCLAF Gene

Aliases for PCLAF Gene

  • PCNA Clamp Associated Factor 2 3
  • KIAA0101 2 3 4 5
  • Hepatitis C Virus NS5A-Transactivated Protein 9 3 4
  • Overexpressed In Anaplastic Thyroid Carcinoma 1 3 4
  • HCV NS5A-Transactivated Protein 9 3 4
  • PCNA-Associated Factor Of 15 KDa 3 4
  • PCNA-Associated Factor 2 3
  • NS5ATP9 3 4
  • OEATC-1 3 4
  • P15PAF 3 4
  • PAF15 3 4
  • PAF 3 4
  • P15(PAF) 3
  • P15/PAF 3
  • OEATC1 3
  • OEATC 3
  • L5 3

External Ids for PCLAF Gene

Previous HGNC Symbols for PCLAF Gene

  • KIAA0101

Summaries for PCLAF Gene

GeneCards Summary for PCLAF Gene

PCLAF (PCNA Clamp Associated Factor) is a Protein Coding gene. Diseases associated with PCLAF include Posterolateral Myocardial Infarction and Hepatitis C Virus. Among its related pathways are Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template and DNA Double-Strand Break Repair. An important paralog of this gene is ENSG00000259316.

UniProtKB/Swiss-Prot for PCLAF Gene

  • PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.

Gene Wiki entry for PCLAF Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PCLAF Gene

Genomics for PCLAF Gene

Regulatory Elements for PCLAF Gene

Enhancers for PCLAF Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH15F064354 1 ENCODE 12.7 +31.7 31667 2.9 HDGF PKNOX1 ATF1 ZFP64 ARID4B SIN3A ETS1 GLIS2 ELK1 KLF7 TRIP4 PCLAF CILP CSNK1G1 GC15P067933
GH15F064618 1 Ensembl ENCODE 5.2 -234.2 -234178 7.3 HDGF PKNOX1 TBL1XR1 SIN3A YBX1 ZNF335 ZNF121 ZNF263 SP3 TBX21 TRIP4 RBPMS2 RNU6-549P PCLAF SNX1 RPL21P15 RPS26P50
GH15F064601 1.1 Ensembl ENCODE 5.2 -214.5 -214515 1.2 HDGF ATF1 PKNOX1 CREB3L1 ARID4B YY1 ZNF766 ZNF207 FOS NFYC ZNF609 RNU6-549P ENSG00000252774 RBPMS2 PCLAF ANKDD1A RPS26P50 RPL21P15
GH15F065183 1.1 ENCODE 5 -797.1 -797076 3.0 CREB3L1 WRNIP1 ARID4B SIN3A DMAP1 YY1 ZNF207 ZNF143 KLF13 DEK CILP SPG21 TRIP4 ENSG00000206903 RNU5A-1 PARP16 RNU5B-1 RBPMS2 ZNF609 UBAP1L
GH15F064362 1 FANTOM5 4.3 +25.1 25132 0.3 ENSG00000252774 SNX22 RNU6-549P RBPMS2 PCLAF PIF1 CSNK1G1 ENSG00000259316
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around PCLAF on UCSC Golden Path with GeneCards custom track

Promoters for PCLAF Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001453044 -314 2400 CREB3L1 MLX ZFP64 FEZF1 DMAP1 YY1 SLC30A9 ZNF143 ZNF263 SP3

Genomic Location for PCLAF Gene

Chromosome:
15
Start:
64,364,311 bp from pter
End:
64,387,687 bp from pter
Size:
23,377 bases
Orientation:
Minus strand

Genomic View for PCLAF Gene

Genes around PCLAF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PCLAF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PCLAF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PCLAF Gene

Proteins for PCLAF Gene

  • Protein details for PCLAF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q15004-PAF15_HUMAN
    Recommended name:
    PCNA-associated factor
    Protein Accession:
    Q15004
    Secondary Accessions:
    • A6NNU5
    • A8K3Y3
    • G9G694
    • G9G696

    Protein attributes for PCLAF Gene

    Size:
    111 amino acids
    Molecular mass:
    11986 Da
    Quaternary structure:
    • Interacts (when monoubiquitinated at Lys-15 and Lys-24) with PCNA. Interacts with isoform 2/p33ING1b of ING1. Interacts with BRCA1.
    Miscellaneous:
    • Overexpression in adrenocortical neoplasms (ACC), may promote growth and invasion in adrenal cancer.
    SequenceCaution:
    • Sequence=BAA03491.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for PCLAF Gene

    Alternative splice isoforms for PCLAF Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PCLAF Gene

Post-translational modifications for PCLAF Gene

  • Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for PCLAF Gene

No data available for DME Specific Peptides for PCLAF Gene

Domains & Families for PCLAF Gene

Protein Domains for PCLAF Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for PCLAF Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q15004

UniProtKB/Swiss-Prot:

PAF15_HUMAN :
  • The PIP-box mediates the interaction with PCNA (PubMed:21628590, PubMed:23000965).
Domain:
  • The PIP-box mediates the interaction with PCNA (PubMed:21628590, PubMed:23000965).
  • The KEN box is required for the association with the APC/C complex.
  • The D-box (destruction box) mediates the interaction with APC/C proteins, and acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
  • The initiation motif is required for efficient chain initiation by the APC/C complex E2 ligase UBE2C. It determines the rate of substrates degradation without affecting its affinity for the APC/C, a mechanism used by the APC/C to control the timing of substrate proteolysis during the cell cycle (PubMed:21700221).
genes like me logo Genes that share domains with PCLAF: view

No data available for Gene Families for PCLAF Gene

Function for PCLAF Gene

Molecular function for PCLAF Gene

UniProtKB/Swiss-Prot Function:
PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.
UniProtKB/Swiss-Prot Induction:
By UV irradiation. By ATF3 in response to UV-stress.

Gene Ontology (GO) - Molecular Function for PCLAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003682 chromatin binding IDA 23000965
GO:0005515 protein binding IPI 21628590
genes like me logo Genes that share ontologies with PCLAF: view
genes like me logo Genes that share phenotypes with PCLAF: view

Animal Models for PCLAF Gene

MGI Knock Outs for PCLAF:

Animal Model Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PCLAF Gene

Localization for PCLAF Gene

Subcellular locations from UniProtKB/Swiss-Prot for PCLAF Gene

Nucleus. Cytoplasm, perinuclear region. Note=Following DNA damage, localizes to DNA damage sites (PubMed:21628590). Colocalizes with centrosomes in perinuclear region (PubMed:21673012).

Gene Ontology (GO) - Cellular Components for PCLAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 21628590
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IDA --
GO:0048471 perinuclear region of cytoplasm IEA,IDA 21673012
genes like me logo Genes that share ontologies with PCLAF: view

No data available for Subcellular locations from COMPARTMENTS for PCLAF Gene

Pathways & Interactions for PCLAF Gene

genes like me logo Genes that share pathways with PCLAF: view

SIGNOR curated interactions for PCLAF Gene

Is activated by:

Gene Ontology (GO) - Biological Process for PCLAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006260 DNA replication IDA 23000965
GO:0006281 DNA repair IEA --
GO:0006974 cellular response to DNA damage stimulus IEA,IDA 21628590
GO:0009411 response to UV IDA 23000965
GO:0019985 translesion synthesis TAS --
genes like me logo Genes that share ontologies with PCLAF: view

Transcripts for PCLAF Gene

Unigene Clusters for PCLAF Gene

KIAA0101:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PCLAF Gene

No ASD Table

Relevant External Links for PCLAF Gene

GeneLoc Exon Structure for
PCLAF
ECgene alternative splicing isoforms for
PCLAF

Expression for PCLAF Gene

NURSA nuclear receptor signaling pathways regulating expression of PCLAF Gene:

PCLAF

SOURCE GeneReport for Unigene cluster for PCLAF Gene:

Hs.81892

mRNA Expression by UniProt/SwissProt for PCLAF Gene:

Q15004-PAF15_HUMAN
Tissue specificity: Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas, adrenocortical carcinomas, and in non-small-cell lung cancer lines.

Primer Products

No data available for mRNA expression in normal human tissues , mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein expression and Protein tissue co-expression partners for PCLAF Gene

Orthologs for PCLAF Gene

This gene was present in the common ancestor of chordates.

Orthologs for PCLAF Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia KIAA0101 34 35
  • 99.1 (n)
cow
(Bos Taurus)
Mammalia PAF 35
  • 91 (a)
OneToOne
KIAA0101 34
  • 90.09 (n)
rat
(Rattus norvegicus)
Mammalia Ns5atp9 34
  • 86.06 (n)
mouse
(Mus musculus)
Mammalia 2810417H13Rik 34 16 35
  • 84.85 (n)
oppossum
(Monodelphis domestica)
Mammalia KIAA0101 35
  • 73 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia KIAA0101 35
  • 70 (a)
OneToOne
dog
(Canis familiaris)
Mammalia KIAA0101 35
  • 63 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia KIAA0101 35
  • 67 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia kiaa0101 34
  • 63.11 (n)
zebrafish
(Danio rerio)
Actinopterygii LOC100333229 34
  • 54.21 (n)
Dr.12145 34
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8724 34
Species where no ortholog for PCLAF was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for PCLAF Gene

ENSEMBL:
Gene Tree for PCLAF (if available)
TreeFam:
Gene Tree for PCLAF (if available)

Paralogs for PCLAF Gene

Paralogs for PCLAF Gene

genes like me logo Genes that share paralogs with PCLAF: view

Variants for PCLAF Gene

Sequence variations from dbSNP and Humsavar for PCLAF Gene

SNP ID Clin Chr 15 pos Sequence Context AA Info Type
rs1033203 -- 64,365,468(-) TAAAC(A/G)GTTGA nc-transcript-variant, utr-variant-3-prime
rs1033204 -- 64,364,626(-) GGCAC(A/G)CACAG downstream-variant-500B
rs1033205 -- 64,364,565(-) ATTCG(C/G)TATCA downstream-variant-500B
rs1033206 -- 64,364,563(-) TCGCT(A/G)TCATA downstream-variant-500B
rs10400921 -- 64,369,738(+) ATTCT(C/T)AATAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PCLAF Gene

Variant ID Type Subtype PubMed ID
dgv1224n106 CNV deletion 24896259
esv1497681 CNV deletion 17803354
esv2319425 CNV deletion 18987734
esv2675981 CNV deletion 23128226
esv2749782 CNV deletion 23290073
esv2749783 CNV deletion 23290073
esv3337124 CNV insertion 20981092
esv3552562 CNV deletion 23714750
esv3636733 CNV loss 21293372
esv3892709 CNV loss 25118596
esv4243 CNV loss 18987735
nsv94581 CNV deletion 16902084

Variation tolerance for PCLAF Gene

Residual Variation Intolerance Score: 52.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.04; 21.30% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for PCLAF Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PCLAF

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PCLAF Gene

Disorders for PCLAF Gene

MalaCards: The human disease database

(8) MalaCards diseases for PCLAF Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
posterolateral myocardial infarction
hepatitis c virus
  • hepatitic c virus
lateral myocardial infarction
  • lateral myocardial infarction nos
heart conduction disease
  • heart rhythm disease
erysipeloid
  • infection due to erysipelothrix rhusiopathiae
- elite association - COSMIC cancer census association via MalaCards
Search PCLAF in MalaCards View complete list of genes associated with diseases

Relevant External Links for PCLAF

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
PCLAF
genes like me logo Genes that share disorders with PCLAF: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PCLAF Gene

Publications for PCLAF Gene

  1. p15(PAF), a novel PCNA associated factor with increased expression in tumor tissues. (PMID: 11313979) Yu P. … Luo Y. (Oncogene 2001) 2 3 4 22 64
  2. The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b. (PMID: 16288740) Simpson F. … Wicking C. (Exp. Cell Res. 2006) 2 3 4 64
  3. Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. (PMID: 23000965) Povlsen L.K. … Choudhary C. (Nat. Cell Biol. 2012) 3 4 64
  4. Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyclosome substrate. (PMID: 21628590) Emanuele M.J. … Elledge S.J. (Proc. Natl. Acad. Sci. U.S.A. 2011) 3 4 64
  5. KIAA0101 interacts with BRCA1 and regulates centrosome number. (PMID: 21673012) Kais Z. … Parvin J.D. (Mol. Cancer Res. 2011) 3 4 64

Products for PCLAF Gene

Sources for PCLAF Gene

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