PCDHAC1 Gene
protein-coding GIFtS: 37
GCID: GC05P140424
|
|
protocadherin alpha subfamily C, 1
| |
Aliases
for PCDHAC1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Protocadherin Alpha Subfamily C, 11 2 | | PCDH-ALPHA-C12 | | Protocadherin Alpha-C12 | | PCDH-Alpha-C11 |
Export aliases for PCDHAC1 gene to outside databasesPrevious GC identifers: GC05U990126 GC05P140149 GC05P140214 GC05P140221 GC05P140176 GC05P140178 GC05P140286 GC05P140252 GC05P140261 GC05P140271 GC05P140280 GC05P140291 GC05P140301 GC05P140310 GC05P140313 GC05P140320 GC05P140343 GC05P135451 GC05P140354 GC05P140375 GC05P140394 GC05P140408 |
Summaries
for PCDHAC1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for PCDHAC1:
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked onchromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor geneclusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes andconsists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons,or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes inthe cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exonsencode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteinsthat most likely play a critical role in the establishment and function of specific cell-cell connections in thebrain. Alternative splicing has been observed and additional variants have been suggested but their full-length naturehas yet to be determined. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: PCDC1_HUMAN, Q9H158Function: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance ofspecific neuronal connections in the brain
|
Genomic Views
for PCDHAC1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the PCDHAC1 gene promoter:
GR SRF AML1a SRF (504 AA) GATA-1 HOXA5 SEF-1 (1) Msx-1
Other transcription factors
Search SABiosciences Chromatin IP Primers for PCDHAC1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PCDHAC1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 5q31 Ensembl cytogenetic band: 5q31.3 HGNC cytogenetic band: 5q31PCDHAC1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 5 GeneLoc Exon Structure GeneLoc location for GC05P140424: view genomic region
(about GC identifiers)
Start:
|
140,306,302 bp from pter |
End:
|
140,391,929 bp from pter |
Size:
|
85,628 bases |
Orientation:
|
plus strand |
|
Proteins
for PCDHAC1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: PCDC1_HUMAN, Q9H158 (See
protein sequence)Recommended Name: Protocadherin alpha-C1 precursor Size: 963 amino acids; 103942 Da
Subcellular location: Cell membrane; Single-pass type I membrane protein (By similarity)
Secondary accessions: Q9Y5F5 Q9Y5I5Alternative splicing: 2 isoforms: Q9H158-1 Q9H158-2 Explore the universe of human proteins at neXtProt for PCDHAC1: NX_Q9H158
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9H158
PCDHAC1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_061721.2 NP_114088.2
ENSEMBL proteins: ENSP00000253807 ENSP00000386356
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table
PCDHAC1 for ontologies About GeneDecksing
PCDHAC1 Antibody Products:
Assay Products for PCDHAC1: |
Protein
Domains /
Families
for PCDHAC1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
PCDHAC1 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9H158ProtoNet protein and cluster: Q9H158 1 Blocks protein family: IPB002126 Cadherin
UniProtKB/Swiss-Prot: PCDC1_HUMAN, Q9H158Similarity: Contains 6 cadherin domains |
Function
for PCDHAC1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: PCDC1_HUMAN, Q9H158Function: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance ofspecific neuronal connections in the brain
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PCDHAC1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PCDHAC1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): PCDHAC1 (NM_031882) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PCDHAC1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PCDHAC1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PCDHAC1 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005509 | calcium ion binding |
IEA | -- |
PCDHAC1 for ontologies About GeneDecksing
Animal Models:
1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for Pcdhac1):
PCDHAC1 for phenotypes About GeneDecksing
|
Pathways & Interactions
for PCDHAC1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for PCDHAC1
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
2 Interacting proteins for PCDHAC1 (ENSP000002538074) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table
PCDHAC1 for ontologies About GeneDecksing
|
Drugs & Compounds
for PCDHAC1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for PCDHAC1
1 HMDB Compound for PCDHAC1 About this table
Search CenterWatch for drugs/clinical trials and news about PCDHAC1 / PCDC1
|
Transcripts
for PCDHAC1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for PCDHAC1 gene (2 alternative transcripts): NM_018898.3 NM_031882.2 2 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000253807(uc003lih.2) ENST00000409700(uc003lig.1)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PCDHAC1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PCDHAC1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): PCDHAC1 (NM_031882) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PCDHAC1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PCDHAC1 |
24/261 AceView cDNA sequences (see all 261): AF152476 NM_031497 AW152471 NM_018899 AF152483 NM_031849 BF933281 NM_031882 BM129562 NM_018907 NM_018911 NM_018903 CA488079 NM_031860 AF152484 NM_031861 BM970705 NM_018906 AF152304 NM_031411 AA384862 AF152477 AF152312 NM_031496
GeneLoc Exon Structure
|
Expression
for PCDHAC1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| PCDHAC1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See PCDHAC1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for PCDHAC1
SABiosciences Custom PCR Arrays for PCDHAC1
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PCDHAC1
Browse OriGene validated miRNA SYBR primer pairs
| | | Search SABiosciences RT2 qPCR
Primer Assays in human, mouse, rat PCDHAC1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PCDHAC1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PCDHAC1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PCDHAC1 |
Orthologs
for PCDHAC1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for PCDHAC1 gene from 3/10 species (see all 10) About this table
ENSEMBL Gene Tree for PCDHAC1 (if available)
TreeFam Gene Tree for PCDHAC1 (if available) |
Paralogs
for PCDHAC1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for PCDHAC1 gene
- PCDHB162 PCDHB22 PCDHAC22 PCDHB72 PCDHB152 PCDHGA102 PCDHGA122 PCDHGB22
- PCDHGA82 PCDHGA92 PCDHGB32 PCDHGC32 PCDHGB62 PCDHA62 PCDHGA52 PCDHA82
- PCDHA132 PCDHB52 PCDHB132 PCDHA32 PCDHGA42 PCDHGA72 PCDHA42 PCDHB42
- PCDHB112 PCDHA22 ENSG000002556222 PCDHB142 PCDHB122 PCDHA122 PCDHB82 PCDHA52
- PCDHGB12 PCDHGB42 PCDHA12 PCDHGA22 PCDHA112 PCDHA72 PCDHGA112 PCDHGA62
- PCDHB32 PCDHB62 PCDHGC52 PCDHGC42 PCDHA102 PCDHA92 PCDHGA12 PCDHB102
- PCDHB12 PCDHGA32 PCDHGB72
18/64 SIMAP similar genes for PCDHAC1 using alignment to 2 protein entries: PCDC1_HUMAN (see all proteins)
(see all similar genes):PCDHGA3 PCDHAC2 DKFZp586I1919 PCDHB18 PCDHGA11 PCDHA3
PCDHA6 PCDHA8 PCDHB15 PCDHGA1 PCDHGB1 PCDH10
PCDHA1 PCDHA10 PCDHA2 PCDHA4 PCDHA7 PCDHB12
PCDHAC1 for paralogs About GeneDecksing
|
Genomic Variants
for PCDHAC1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for PCDHAC1
2 CNVs: 99310 3578 | SABiosciences Cancer Mutation PCR Assays |
|
Disorders
/ Diseases
for PCDHAC1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
PCDHAC1 for disorders About GeneDecksing
OMIM gene information: 606320
OMIM disorders: --
1  disease for PCDHAC1: About MalaCardsneuronitis
Export disorders for PCDHAC1 gene to outside databases
|
Publications
for PCDHAC1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for PCDHAC1 gene integrated from 9 sources:
(articles sorted by number of sources associating them with PCDHAC1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- A striking organization of a large family of human neural cadherin- like cell adhesion genes. (PubMed id 10380929)1, 2, 3 Wu Q. and Maniatis T. (1999)
- Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women. (PubMed id 22589738)1 Fox C.S....Borecki I.B. (2012)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Comparative DNA sequence analysis of mouse and human protocadherin gene clusters. (PubMed id 11230163)1 Wu Q.... Maniatis T. (2001)
- Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members. (PubMed id 10835267)1 Nollet F....van Roy F. (2000)
- Genomic organization of the family of CNR cadherin genes in mice and humans. (PubMed id 10662547)1 Sugino H....Yagi T. (2000)
- Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes. (PubMed id 10716726)1 Wu Q. and Maniatis T. (2000)
- Cadherin superfamily genes: functions, genomic organization, and neurologic diversity. (PubMed id 10817752)1 Yagi T. and Takeichi M. (2000)
|
External Searches for PCDHAC1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing PCDHAC1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing PCDHAC1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PCDHAC1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for PCDHAC1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for PCDHAC1 gene:
Search GeneIP for patents involving PCDHAC1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for PCDHAC1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for PCDHAC1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PCDHAC1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PCDHAC1 | | | OriGene Protein Over-expression Lysate for PCDHAC1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for PCDHAC1 | | OriGene 3'-UTR Clone for PCDHAC1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PCDHAC1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PCDHAC1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for PCDHAC1 | | OriGene Custom Protein Services for PCDHAC1 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat PCDHAC1 | | | Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PCDHAC1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PCDHAC1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PCDHAC1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PCDHAC1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PCDHAC1 |
| |
 |  |  |  | |
| | | | Search Tocris compounds for PCDHAC1 |
| |  |  |  |  | | | | |
 |
 |
 |
 | | | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PCDHAC1 |
|  |  |  | | | | Search ThermoFisher Antibodies for PCDHAC1 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PCDHAC1 |
|
|
|
|
