 |
 | Services
| |
 |
Aliases & Descriptions for PAK2
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| EC 2.7.11.1 3 | | Gamma-PAK 3 | | PAK-2 3 | | PAK65 1, 2, 3, 5 | | PAKgamma 1, 2 | | p58 3 |
| | | Descriptions |
|---|
| S6/H4 kinase 2, 3 | | p21 (CDKN1A)-activated kinase 2 1, 2 | | p21 protein (Cdc42/Rac)-activated kinase 2 2 | | p21-activated kinase 2 2, 3 |
|
| | Search outside databases for aliases for PAK2 genePrevious GC identifers: GC15P018867 GC03P196858 GC03P197874 GC03P197793 |
Summaries for PAK2(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for PAK2: The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq] UniProtKB/Swiss-Prot: PAK2_HUMAN, Q13177Function: The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated formGene Wiki entry for PAK2 |
Genomic Location for PAK2
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the PAK2 gene 
Entrez Gene cytogenetic band: 3q29 Ensembl cytogenetic band: 3q29 HGNC cytogenetic band: 3q29PAK2 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03P197955:
(about GC identifiers)
Start:
|
197,951,312 bp from pter |
End:
|
198,043,756 bp from pter |
Size:
|
92,445 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000003.10 NT_029928.12
| Proteins for PAK2
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: PAK2_HUMAN, Q13177 (See
protein sequence)Recommended Name: Serine/threonine-protein kinase PAK 2 Size: 524 amino acids; 58043 Da
Subunit: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with SH3MD4. Interacts with and activated by HIV-1 Nef. PAK-2p34 interacts with ARHGAP10
Subcellular location: Serine/threonine-protein kinase PAK 2: Cytoplasm
Subcellular location: PAK-2p34: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Note=Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane
Secondary accessions: Q13154 Q6ISC3Post-translational modifications:
Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate1
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p341
Ubiquitinated, leading to its proteosomal degradation1
PAK-2p34 is myristoylated1
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_002568.2
ENSEMBL proteins: ENSP00000314067
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
4 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for PAK2: Assays for PAK2: | Protein
Domains/ Families for PAK2(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q13177
ProtoNet protein and cluster: Q13177 1 Blocks protein family: IPB000095 PAK-box/P21-Rho-binding
UniProtKB/Swiss-Prot: PAK2_HUMAN, Q13177Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamilySimilarity: Contains 1 CRIB domainSimilarity: Contains 1 protein kinase domain | Gene Function for PAK2
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002577
Applied Biosystems Silencer® siRNAs for PAK2
Sigma-Aldrich siRNA and siRNA Panels for PAK2  Sigma-Aldrich shRNA Panels and shRNA for PAK2  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002577                                  untagged cDNA clone in CMV expression vector: XM_039354 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_002577
UniProtKB/Swiss-Prot: PAK2_HUMAN, Q13177Function: The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated formCatalytic activity: ATP + a protein = ADP + a phosphoproteinEnzyme regulation: Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage, autophosphorylted PAK-2p34 is constitutively activeEnzyme Number (IUBMB): EC 2.7.11.1 5 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions for PAK2
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
5/20 Sigma-Aldrich "Your Favorite Gene" Pathways for PAK2 (Your Favorite Gene powered by Ingenuity) (see all 20
) 
Gene Network CentralTM Interacting Genes and Proteins Network for PAK2 
5/118 Interacting proteins for PAK2 (ENSP000003140673 Q131771, 2) via UniProtKB, MINT, and/or STRING (see all 118
)About this table
5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6
): About this table
|
Drugs & Compounds for PAK2(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for PAK2 10/14 Novoseek chemical compound relationships for PAK2 gene (see all 14
)
About this table
|
Transcripts for PAK2(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002577
Sigma-Aldrich siRNA and siRNA Panels for PAK2  Sigma-Aldrich shRNA Panels and shRNA for PAK2  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_002577 REFSEQ mRNAs for PAK2 gene: NM_002577.4
Applied Biosystems TaqMan ® Gene Expression Assays: NM_002577               OriGene GFP tagged cDNA clone in CMV expression vector: NM_002577                                  untagged cDNA clone in CMV expression vector: XM_039354  Additional cDNA sequence: AK291339.1 BC031667.1 BC046197.2 BC063539.1 BC069613.1 CR600384.1 CR620221.1 CR624656.1 DQ786200.1 U25975.1 21 DOTS entries: DT.95277844 DT.92450523 DT.75117060 DT.99974345 DT.91788405 DT.92450517 DT.97792643 DT.100723254 DT.100824650 DT.101960572 DT.86835945 DT.91785574 DT.92450532 DT.95216865 DT.95277845 DT.97808984 DT.97855397 DT.100699230 DT.100824652 DT.121053025 DT.95239182 24/372 AceView cDNA sequences (see all 372
):BQ424929 AW575807 AI140979 BE047085 AA262968 BM698698 CD644040 AA999820 AI918910 BM670792 AW673916 AW337613 BM541651 AU131426 AI190809 BX505834 BE466478 BM829142 AA992535 AA552526 BU685000 AI080323 CD722577 BE503920
highest scoring ESTs for PAK2:N25547 U25975 W74494 AA004735 AA127227 AA127228 AA195194 AA195310 AA236312 AA262968 Unigene Cluster for PAK2: P21 protein (Cdc42/Rac)-activated kinase 2 Hs.518530 [show with all ESTs]Unigene Representative Sequence: NM_002577
GeneLoc Exon Structure
1 Ensembl transcript including schematic representation: ENST00000327134
|
Expression for PAK2
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| PAK2 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for PAK2
1 / 2 / 3 19 probe-sets matching PAK2 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: --
SOURCE GeneReport for Unigene cluster: Hs.518530
Expression variation in blood from EXPOLDB for PAK2 UniProtKB/Swiss-Prot: PAK2_HUMAN, Q13177Tissue specificity: Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen |
Orthologs for PAK2
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for PAK2 gene from 5/9 species (see all 9
)
About this table Species with no ortholog for PAK2
ENSEMBL Gene Tree for PAK2 | Paralogs for PAK2(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for PAK2 gene
- PAK32 PAK12
Pseudogenes
- 1 related pseudogene
|
SNPs/Variants for PAK2(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for PAK2 (up to first 250kb)
|
Disorders & Mutations for PAK2
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 605022
8 Novoseek disease relationships for PAK2 gene
| Disease |
Score |
Articles |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| immunodeficiency |
29.54 |
14 |
11160719 (2), 17881449 (1), 18094167 (1), 16407193 (1) (see all 7) |
| necrosis |
0.00 |
3 |
9624143 (1), 11278362 (1), 12853446 (1) |
| shock |
0.00 |
7 |
9717744 (3), 9989786 (2) |
| cancer |
0.00 |
1 |
15072767 (1) |
| carcinoma |
0.00 |
2 |
15095415 (1), 10797567 (1) |
| aids |
0.00 |
1 |
17881449 (1) |
| tumors |
0.00 |
9 |
19289088 (3), 9624143 (1), 12853446 (1), 11278362 (1) |
| virus infection |
0.00 |
1 |
16501114 (1) |
About this table
|
Medical News for PAK2(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for PAK2 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/154 PubMed articles for PAK2 gene (see all 154
):- A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20. (PubMed id 7744004)2, 3, 4 Martin G.A.... Abo A. (1995)
- Lentivirus Nef specifically activates Pak2. (PubMed id 11070003)1, 3, 4 Arora V.K.... Garcia J.V. (2000)
- Caspase-activated PAK-2 is regulated by subcellular targeting and proteasomal degradation. (PubMed id 12853446)1, 3, 4 Jakobi R.... Stringer D.K. (2003)
- Identification and characterization of PS-GAP as a novel regulator of caspase-activated PAK-2. (PubMed id 15471851)1, 3, 4 Koeppel M.A.... Jakobi R. (2004)
- Cleavage and activation of p21-activated protein kinase gamma-PAK by CPP32 (caspase 3). Effects of autophosphorylation on activity. (PubMed id 9786869)1, 3, 4 Walter B.N.... Traugh J.A. (1998)
- Posttranslational myristoylation of caspase-activated p21-activated protein kinase 2 (PAK2) potentiates late apoptotic events. (PubMed id 16617111)1, 3, 4 Vilas G.L....Berthiaume L.G. (2006)
- Activation of an S6/H4 kinase (PAK 65) from human placenta by intramolecular and intermolecular autophosphorylation. (PubMed id 7673144)1, 3, 4 Benner G.E.... Masaracchia R.A. (1995)
- A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)3, 4 Beausoleil S.A.... Gygi S.P. (2006)
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)3, 4 Olsen J.V....Mann M. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
|
Search for PAK2
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing PAK2
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing PAK2
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PAK2(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for PAK2(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for PAK2:

 | |
 | | | | |
|
 |  |  |
| Antibodies & Assays for PAK2  |
| | | | Search Tocris compounds for PAK2 |
|
 |
 | | | |
GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009
|