V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

PAK1IP1 Gene

protein-coding   GIFtS: 56

GC06P010803
PAK1 interacting protein 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FLJ20624 1, 2
MAK11 1, 2
OTTHUMP00000016022 2
PIP1 1, 2, 3, 5
RP11-421M1.5 2
WDR84 1, 2, 3
bA421M1.5 1, 2
hPIP1 1, 2, 3
Descriptions
PAK/PLC-interacting protein 1 2, 3
PAK1 interacting protein 1 2
PAK1-interacting protein 2
PAK1-interacting protein 1 3
WD repeat-containing protein 84 3
External Ids
HGNC: 208821
Entrez Gene: 550032
UniProtKB: Q9NWT13
Ensembl: ENSG000001118457
Search outside databases for aliases for PAK1IP1 gene

Previous GC identifer: GC06P010757

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: PK1IP_HUMAN, Q9NWT1
Function: Negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which
have been shown to play a positive role in the regulation of signaling pathways involving MAPK8
and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases
such as CDC42 to an N-terminal regulatory domain. PAK1IP1 also binds to the N-terminus of PAK1,
and inhibits the specific activation of PAK1 by CDC42

Gene Wiki entry for PAK1IP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the PAK1IP1 gene  

Entrez Gene cytogenetic band: 6p24.2   Ensembl cytogenetic band:  6p24.2   HGNC cytogenetic band: 6p24.1

PAK1IP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P010803:     (about GC identifiers)

Start:
10,803,174 bp from pter
End:
10,817,958 bp from pter
Size:
14,785 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000006.10  NT_007592.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: PK1IP_HUMAN, Q9NWT1 (See protein sequence)
Recommended Name: p21-activated protein kinase-interacting protein 1  
Size: 392 amino acids; 43964 Da
Subunit: Interacts with PAK1
Subcellular location: Nucleus, nucleolus
Secondary accessions: Q5T4J2 Q96QJ8 Q96T87

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_060376.2  

    ENSEMBL proteins: 
    ENSP00000368887 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (PAK1 interacting protein 1)
    Human Recombinant Proteins from Abnova (PAK1IP1)
                  OriGene Purified Recombinant Human Protein: PAK1IP1 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for PAK1IP1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for PAK1IP1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (PAK1 interacting protein 1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (PAK1IP1)
    Novus Biologicals Antibodies for PAK1IP1

    Assays for PAK1IP1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/6 InterPro domains/families (see all 6 ):
     IPR019782 WD40_repeat_2
     IPR019775 WD40_repeat_CS
     IPR001680 WD40_repeat
     IPR017986 WD40_repeat_region
     IPR015943 WD40/YVTN_repeat-like


       GeneDecks  PAK1IP1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9NWT1

    ProtoNet protein and cluster: Q9NWT1

    UniProtKB/Swiss-Prot: PK1IP_HUMAN, Q9NWT1
    Similarity: Contains 5 WD repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PAK1IP1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_017906

                  Applied Biosystems Silencer® siRNAs for PAK1IP1

                  Sigma-Aldrich siRNA for PAK1IP1  
                         Sigma-Aldrich shRNA for PAK1IP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for PAK1IP1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_017906
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_017906
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_017906 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_017906

    UniProtKB/Swiss-Prot: PK1IP_HUMAN, Q9NWT1
    Function: Negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which
    have been shown to play a positive role in the regulation of signaling pathways involving MAPK8
    and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases
    such as CDC42 to an N-terminal regulatory domain. PAK1IP1 also binds to the N-terminus of PAK1,
    and inhibits the specific activation of PAK1 by CDC42

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  PAK1IP1 


    5/237 Interacting proteins for PAK1IP1 (Q9NWT12 ENSP000003688873) via UniProtKB, MINT, and/or STRING (see all 237 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ATAD3BQ5T9A42MINT-6488791
    COPAP536212MINT-6488791
    DDX18Q9NVP12MINT-6488791
    DDX39O001482MINT-6488791
    DDX47Q9H0S42MINT-6488791
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0009968 negative regulation of signal transduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for PAK1IP1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PAK1IP1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_017906

                  Sigma-Aldrich siRNA for PAK1IP1  
                         Sigma-Aldrich shRNA for PAK1IP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_017906  

    REFSEQ mRNAs for PAK1IP1 gene: 

    NM_017906.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_017906  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_017906
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_017906
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_017906 

    Additional cDNA sequence: 

    AF283303.1 AK000631.1 AK225516.1 BC010907.1 

    7 DOTS entries:

    DT.412342  DT.95168625  DT.100814008  DT.121391295  DT.86855075  DT.121391278  DT.75102633 

    24/127 AceView cDNA sequences (see all 127 ):

    AW117624 BI545887 AI393969 N53359 BU181468 H62065 AI537089 AI872448 
    BQ226963 BU618231 CD557340 AW592352 AI141270 BE504340 BM973899 BU177530 
    BM756293 BU677437 AA236103 AA953892 BU629319 AA679804 AI632312 BQ216070 

    highest scoring ESTs for PAK1IP1:

    AI521868 AA126597 AA157015 AA228341 AA534957 AA564151 AA640001 AA679804 AF283303 AI141270 

    Unigene Cluster for PAK1IP1:

    PAK1 interacting protein 1
    Hs.310231  [show with all ESTs]
    Unigene Representative Sequence: AK000631


    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for PAK1IP1

    ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10a · 10b
    SP1:                                                                                          
    SP2:                                                                                          
    SP3:                                -     -     -     -     -                                 

    About this scheme

    ECgene alternative splicing isoforms for PAK1IP1

    1 Ensembl transcript including schematic representation:
    ENST00000379568  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    PAK1IP1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for PAK1IP1

    1 / 2 / 3

    3 probe-sets matching PAK1IP1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    53034_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI521868 1.00 1.00 1.00 1

    218886_at2, 3 U133-A 1 1.00 1.00 -- -- NM_017906 0.60 1.00 0.82 1

    218886_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  PAK1IP1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TAATTTTTAC

    SOURCE GeneReport for Unigene cluster: Hs.310231

    UniProtKB/Swiss-Prot: PK1IP_HUMAN, Q9NWT1
    Tissue specificity: Expressed in brain, colon, heart, kidney, liver, lung, muscle, peripheral blood
    leukocytes, placenta, small intestine, spleen and thymus

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for PAK1IP1 gene from 5/19 species (see all 19 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    PAK1IP11   -- PAK1 interacting protein 1 88.8(n)
    85.53(a)
    478719  XM_535884.2  XP_535884.1 
    chimpanzee
    (Pan troglodytes)
    PAK1IP11   -- PAK1 interacting protein 1 99.49(n)
    99.23(a)
    463143  XM_518863.2  XP_518863.2 
    cow
    (Bos taurus)
    PAK1IP11   -- PAK1 interacting protein 1 84.61(n)
    81.63(a)
    505125  NM_001014852.1  NP_001014852.1 
    rat
    (Rattus norvegicus)
    Pak1ip11   -- PAK1 interacting protein 1 82.24(n)
    76.9(a)
    361232  NM_001037356.1  NP_001032433.1 
    mouse
    (Mus musculus)
    Pak1ip11, 5 135
    PAK1 interacting protein 11, 5 79.97(n)1
    74.02(a)1
    680831  NM_026550.21  NP_080826.21 
     AA0331325  AA4198255  (see all 33)
    About this table        Species with no ortholog for PAK1IP1

    ENSEMBL Gene Tree for PAK1IP1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/105 NCBI SNPs in PAK1IP1 are shown (see all 105 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 33)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 6 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs77458561,2
    A,C,F,H10803144(+) TTTGGT/CTTCCG 1 -- ng51 ese37Minor allele frequency- C:0.09EU EA WA NA 560
    rs94672521,2
    A,F,H10802794(+) AATGCA/GCGCTG 1 -- ng514Minor allele frequency- G:0.17EU EA WA 418
    --
    rs109467081,2
    A,C10801518(+) caattA/Gagcac 1 -- ng510--------
    rs94610461,2
    F,H10801692(+) CTAGCC/TTCTAT 1 -- ng514Minor allele frequency- T:0.03EU EA WA 416
    rs37987061,2
    H10817929(+) ACTTTG/ATACAA 1 -- ut314Minor allele frequency- A:0.00EU EA WA 418
    --
    rs11298561,2
    F10817803(+) aaaCCA/CCTTTT 1 -- ut311Minor allele frequency- C:0.01MN 184
    --
    rs178563401,2
    H10812830(+) GCACCA/GTCACA 1 I/V mis1 ese34Minor allele frequency- G:0.00EU EA WA 416
    rs69129931,2
    A,C,F,H10817061(+) TGAATA/CTCATA 1 -- int17Minor allele frequency- C:0.25EU EA WA NA 556
    rs77422201,2
    A,C,F,H10803477(+) AGGCAA/GTATCT 1 -- int14Minor allele frequency- G:0.10EU EA WA 412
    rs69390981,2
    A,C,F,H10810995(+) AGAATG/AAAAAT 1 -- int17Minor allele frequency- A:0.11EU EA WA NA 554
    About this table

    HapMap Linkage Disequilibrium images for PAK1IP1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607811

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/11 PubMed articles for PAK1IP1 gene (see all 11 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55003 HGNC: 20882 AceView: PAK1IP1 Ensembl:ENSG00000111845 euGenes: HUgn55003
    ECgene: PAK1IP1 H-InvDB: PAK1IP1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for PAK1IP1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for PAK1IP1-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for PAK1IP1 Antibodies for PAK1IP1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for PAK1IP1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (PAK1IP1)
     Antibodies (PAK1IP1)
     Search for Chimera RNAi
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (PAK1 interacting protein 1)
     Antibodies (PAK1 interacting protein 1)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for PAK1IP1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for PAK1IP1

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    PAK1IP1 Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com