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Aliases & Descriptions for PAFAH2
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| EC 3.1.1.47 3 | | FLJ26025 2 | | HSD-PLA2 1, 2, 3 | | OTTHUMP00000008596 2 |
| | | Descriptions |
|---|
| Serine-dependent phospholipase A2 3 | | platelet-activating factor acetylhydrolase 2 2 | | platelet-activating factor acetylhydrolase 2 (40kD) 1, 2 | | platelet-activating factor acetylhydrolase 2, 40kDa 2 |
|
| | Search outside databases for aliases for PAFAH2 genePrevious GC identifers: GC01M025767 GC01M025307 GC01M025520 GC01M025892 GC01M025891 GC01M025971 |
Summaries for PAFAH2(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for PAFAH2: This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. [provided by RefSeq] UniProtKB/Swiss-Prot: PAFA2_HUMAN, Q99487Function: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzymeGene Wiki entry for PAFAH2 |
Genomic Location for PAFAH2
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the PAFAH2 gene 
Entrez Gene cytogenetic band: 1p36 Ensembl cytogenetic band: 1p36.11 HGNC cytogenetic band: 1p34.3PAFAH2 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 1 GeneLoc Exon Structure GeneLoc location for GC01M026158:
(about GC identifiers)
Start:
|
26,158,845 bp from pter |
End:
|
26,197,235 bp from pter |
Size:
|
38,391 bases |
Orientation:
|
minus strand |
RefSeq DNA sequence:- NC_000001.9 NT_004610.18
| Proteins for PAFAH2
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: PAFA2_HUMAN, Q99487 (See
protein sequence)Recommended Name: Platelet-activating factor acetylhydrolase 2, cytoplasmic Size: 392 amino acids; 44036 Da
Subunit: Monomer
Subcellular location: Cytoplasm
Mass spectrometry: Mass=44162; Method=Electrospray; Range=1-392; Source=PubMed:9494101;
Secondary accessions: O15458REFSEQ proteins: NP_000428.2
ENSEMBL proteins: ENSP00000363402 ENSP00000363400
Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: PAFAH2 
2 Gene Ontology (GO) cellular component terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005737 | cytoplasm |
IEA | -- | | GO:0008247 | 1-alkyl-2-acetylglycerophosphocholine esterase complex |
IEA | -- | About this table
Antibodies for PAFAH2: Assays for PAFAH2: | Protein
Domains/ Families for PAFAH2(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q99487
ProtoNet protein and cluster: Q99487 2 Blocks protein families: IPB005065 Platelet-activating factor acetylhydrolase IPB008262 Lipase
UniProtKB/Swiss-Prot: PAFA2_HUMAN, Q99487Similarity: Belongs to the serine esterase family | Gene Function for PAFAH2
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_000437
Applied Biosystems Silencer® siRNAs for PAFAH2
Sigma-Aldrich siRNA and siRNA Panels for PAFAH2  Sigma-Aldrich shRNA for PAFAH2  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_000437                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_000437                                  untagged cDNA clone in CMV expression vector: NM_000437 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_000437
UniProtKB/Swiss-Prot: PAFA2_HUMAN, Q99487Function: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzymeCatalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetateEnzyme regulation: Inhibited by phenylmethanesulfonyl fluoride, 3,4,dichloroisocoumarin, diisopropyl fluorophosphate (DFP) and diethyl p-nitrophenyl phosphate (DENP)Enzyme Number (IUBMB): EC 3.1.1.47 Genatlas biochemistry entry for PAFAH2:platelet activating factor acetylhydrolase 2,widely expressed in serum and tissues,with high activity for oxidized phospholipids3 MGI mutant phenotypes (inferred from 2 alleles ) (MGI details for Pafah2):
3 Gene Ontology (GO) molecular function terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003847 | 1-alkyl-2-acetylglycerophosphocholine esterase activity |
IEA | -- | | GO:0005543 | phospholipid binding |
TAS | 8955149 | | GO:0016787 | hydrolase activity |
IEA | -- | About this table | Pathways & Interactions for PAFAH2
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
1 Gene Ontology (GO) biological process term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016042 | lipid catabolic process |
IEA | -- | About this table
|
Drugs & Compounds for PAFAH2(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for PAFAH2
|
Transcripts for PAFAH2(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_000437
Sigma-Aldrich siRNA and siRNA Panels for PAFAH2  Sigma-Aldrich shRNA for PAFAH2  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_000437 REFSEQ mRNAs for PAFAH2 gene: NM_000437.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_000437               OriGene GFP tagged cDNA clone in CMV expression vector: NM_000437                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_000437                                  untagged cDNA clone in CMV expression vector: NM_000437  Additional cDNA sequence: AK313815.1 BC001158.1 CR597579.1 D87845.1 U89386.1 9 DOTS entries: DT.97844200 DT.91893885 DT.100750209 DT.100750207 DT.95249956 DT.100750212 DT.91751468 DT.100750205 DT.313239 24/141 AceView cDNA sequences (see all 141
):AA297363 BQ688317 AA047674 BQ064351 AL541283 BU147124 BM984497 BQ056797 BU185161 BQ055409 BU171117 BQ056374 BU630496 BQ428031 BU683529 BQ057778 BQ059273 W17006 BQ054573 AI081340 AA298364 CD370919 AA704048 BQ060667
highest scoring ESTs for PAFAH2:D87845 BC001158 BM914579 BQ706209 BQ706374 BQ709400 BQ711177 BQ883500 BU178830 U89386 Unigene Cluster for PAFAH2: Platelet-activating factor acetylhydrolase 2, 40kDa Hs.477083 [show with all ESTs]Unigene Representative Sequence: NM_000437
GeneLoc Exon Structure
5/6 Alternative Splicing Database (ASD) splice patterns (SP) for PAFAH2 (see all 6
)
| ExUns: | 1 | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8a | · | 8b | ^ | 9a | · | 9b | ^ | 10 | ^ | 11 | ^ | 12 | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP5: | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for PAFAH2
2 Ensembl transcripts including schematic representations: ENST00000374284
ENST00000374282
|
Expression for PAFAH2
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| PAFAH2 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for PAFAH2
1 / 2 / 3 6 probe-sets matching PAFAH2 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: --
SOURCE GeneReport for Unigene cluster: Hs.477083
Expression variation in blood from EXPOLDB for PAFAH2 UniProtKB/Swiss-Prot: PAFA2_HUMAN, Q99487Tissue specificity: Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex |
Orthologs for PAFAH2
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for PAFAH2 gene from 5/8 species (see all 8
)
About this table Species with no ortholog for PAFAH2
ENSEMBL Gene Tree for PAFAH2 | Paralogs for PAFAH2(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for PAFAH2 gene
- PLA2G72
|
SNPs/Variants for PAFAH2(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for PAFAH2 (up to first 250kb)
|
Disorders & Mutations for PAFAH2
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 602344
Human Genome Epidemiology Navigator: PAFAH2 (1 document)
|
Medical News for PAFAH2(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for PAFAH2 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10 PubMed articles for PAFAH2 gene:- Expression, purification and characterization of a human serine-dependent phospholipase A2 with high specificity for oxidized phospholipids and platelet activating factor. (PubMed id 9494101)1, 2, 3, 4 Rice S.Q....Tew D.G. (1998)
- cDNA cloning and expression of intracellular platelet-activating factor (PAF) acetylhydrolase II. Its homology with plasma PAF acetylhydrolase. (PubMed id 8955149)2, 3, 4 Hattori K.... Inoue K. (1996)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- Platelet-activating factor acetylhydrolases. (PubMed id 9218411)3, 4 Stafforini D.M.... Prescott S.M. (1997)
- Lipoprotein-associated phospholipase A2 concentrations in plasma are associated with the extent of coronary artery disease and correlate to adipose tissue levels of marine n-3 fatty acids. (PubMed id 17157859)3 Schmidt E.B....Christensen J.H. (2008)
- Neuroprotective role of transgenic PAF-acetylhydrolase II in mouse models of focal cerebral ischemia. (PubMed id 17272759)3 Umemura K....Hiraga K. (2007)
- A single nucleotide polymorphism in the plasma PAF acetylhydrolase gene and risk of atherosclerosis in Japanese patients with peripheral artery occlusive disease. (PubMed id 16650870)3 Unno N....Konno H. (2006)
- Identification of platelet-activating factor acetylhydrolase II in human skin. (PubMed id 12406338)3 Marques M....Travers J.B. (2002)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)3 Strausberg R.L....Marra M.A. (2002)
- Platelet-activating factor acetylhydrolases: broad substrate specificity and lipoprotein binding does not modulate the catalytic properties of the plasma enzyme. (PubMed id 11294621)3 Min J.H....Gelb M.H. (2001)
|
Search for PAFAH2
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing PAFAH2
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing PAFAH2
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PAFAH2(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for PAFAH2(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for PAFAH2:

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Incremental update: 13 Oct 2009
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