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PACSIN3 Gene

protein-coding   GIFtS: 54

GC11M047155
protein kinase C and casein kinase substrate in neurons 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
SDPIII 1, 2
Descriptions
Endophilin I 3
SH3 domain-containing protein 6511 2, 3
protein kinase C and casein kinase substrate in neurons 3 2
syndapin III 2
External Ids
HGNC: 85721
Entrez Gene: 297632
UniProtKB: Q9UKS63
Ensembl: ENSG000001659127
Search outside databases for aliases for PACSIN3 gene

Previous GC identifers: GC11M049138 GC11M048076 GC11M047230 GC11M047163

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: PACN3_HUMAN, Q9UKS6
Function: May play a role in vesicle formation and transport

Gene Wiki entry for PACSIN3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the PACSIN3 gene  

Entrez Gene cytogenetic band: 11p12-p11.12   Ensembl cytogenetic band:  11p11.2   HGNC cytogenetic band: 11p12-p11

PACSIN3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M047155:     (about GC identifiers)

Start:
47,155,654 bp from pter
End:
47,164,534 bp from pter
Size:
8,881 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_009237.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: PACN3_HUMAN, Q9UKS6 (See protein sequence)
Recommended Name: Protein kinase C and casein kinase substrate in neurons protein 3  
Size: 424 amino acids; 48487 Da
Subunit: Homo- and hetero-aggregates with other PACSINs. Binds dynamin 1, synaptojanin, synapsin 1
and the neural Wiskott-Aldrich syndrome protein (N-WASP). Also interacts with MDC9 and MDC15 (By
similarity)
Subcellular location: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Note=Detected at the inner aspect of the plasma membrane in cells or myotubes
Secondary accessions: A6NH84 Q9H331 Q9NWV9

Post-translational modifications:

  • Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) (Probable)1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_057307.2  

    ENSEMBL proteins: 
    ENSP00000298838 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (PACSIN3)
    Human Recombinant Proteins from Abnova (PACSIN3)
                  OriGene Purified Recombinant Human Protein: PACSIN3 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm ISS--
    GO:0005886 plasma membrane IEA--
    About this table

    Antibodies for PACSIN3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (PACSIN3), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (PACSIN3)
    Novus Biologicals Antibody for PACSIN3

    Assays for PACSIN3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR001060 FCH
     IPR001452 SH3_domain


       GeneDecks  PACSIN3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UKS6

    ProtoNet protein and cluster: Q9UKS6

    2 Blocks protein families:
    IPB001060 Cdc15/Fes/CIP4
    IPB001452 SH3 domain signature


    UniProtKB/Swiss-Prot: PACN3_HUMAN, Q9UKS6
    Similarity: Belongs to the PACSIN family
    Similarity: Contains 1 FCH domain
    Similarity: Contains 1 SH3 domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PACSIN3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (PACSIN3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016223

                  Applied Biosystems Silencer® siRNAs for PACSIN3

                  Sigma-Aldrich siRNA for PACSIN3  
                         Sigma-Aldrich shRNA for PACSIN3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_016223
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_016223
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_016223 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_016223

    UniProtKB/Swiss-Prot: PACN3_HUMAN, Q9UKS6
    Function: May play a role in vesicle formation and transport

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008092 cytoskeletal protein binding ISS--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  PACSIN3 


    5/14 Interacting proteins for PACSIN3 (ENSP000002988383 Q9UKS61, 2) via UniProtKB, MINT, and/or STRING (see all 14 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ADAM9Q134431STRING (score=.801) EBI-77926, EBI-77903
    ADAM15Q134441, 2STRING (score=.937) EBI-77926, EBI-77818 MINT-2792521 EBI-77926, EBI-77818 MINT-2792521
    NOL3O609361EBI-77926, EBI-740992
    PEBP1P300861EBI-77926, EBI-716384
    PYGMP112171EBI-77926, EBI-357469
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0045806 negative regulation of endocytosis ISS--
    GO:0051044 positive regulation of membrane protein ectodomain proteolysis IMP15280379
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for PACSIN3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PACSIN3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (PACSIN3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016223

                  Sigma-Aldrich siRNA for PACSIN3  
                         Sigma-Aldrich shRNA for PACSIN3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_016223  

    REFSEQ mRNAs for PACSIN3 gene: 

    NM_016223.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_016223  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_016223
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_016223
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_016223 

    Additional cDNA sequence: 

    AF130979.1 AF149825.1 AF242530.1 AK000577.1 AK300658.1 AK300968.1 AK310819.1 BC007914.2 
    BC011889.2 CR595171.1 CR600161.1 CR601247.1 CR606050.1 CR613894.1 CR624285.1 

    11 DOTS entries:

    DT.212767  DT.100795763  DT.101970421  DT.100795762  DT.95101690  DT.120707370  DT.87014814  DT.120707471 
    DT.40218044  DT.91742427  DT.92053221 

    24/713 AceView cDNA sequences (see all 713 ):

    BM910653 AI197967 AI568284 BM044239 AI689497 CA309915 AI251129 AI140280 
    BQ946737 AU279596 BU630871 CA313336 CR595171 BF513176 CB153664 CR598905 
    CB163548 BQ228103 CB113119 Z38509 BQ070295 F26225 BQ614613 AI598200 

    highest scoring ESTs for PACSIN3:

    AF130979 AF149825 AF242530 BC007914 BC011889 BE727121 BG762641 BM273299 BM545612 BM924792 

    Unigene Cluster for PACSIN3:

    Protein kinase C and casein kinase substrate in neurons 3
    Hs.334639  [show with all ESTs]
    Unigene Representative Sequence: AF130979


    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for PACSIN3 (see all 10 )

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c ^ 7a · 7b ^ 8a · 8b · 8c ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14a · 14b
    SP1:              -     -     -     -           -                                                                                             
    SP2:              -     -     -     -           -                                                                                             
    SP3:                    -     -     -           -                                                                                             
    SP4:              -     -     -     -           -     -     -                                                                                 
    SP5:              -     -     -     -           -     -     -                                                                                 

    About this scheme

    ECgene alternative splicing isoforms for PACSIN3

    1 Ensembl transcript including schematic representation:
    ENST00000298838  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    PACSIN3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for PACSIN3

    1 / 2 / 3

    4 probe-sets matching PACSIN3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    218744_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_016223 0.60 0.83 0.73 1

    222227_at2 U133-A 6 0.55 0.33 -- -- -- -- -- -- --

    218744_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222227_at2 U133Plus2 6 0.55 0.33 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AACTGGCCAC

    SOURCE GeneReport for Unigene cluster: Hs.334639

    UniProtKB/Swiss-Prot: PACN3_HUMAN, Q9UKS6
    Tissue specificity: Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and
    placenta

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for PACSIN3 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    PACSIN31   -- protein kinase C and casein kinase substrate in neurons more 91.51(n)
    96.46(a)
    475984  XM_856069.1  XP_861162.1 
    chimpanzee
    (Pan troglodytes)
    PACSIN31   -- protein kinase C and casein kinase substrate in neurons more 98.9(n)
    99.06(a)
    451163  XM_001166760.1  XP_001166760.1 
    cow
    (Bos taurus)
    PACSIN31   -- similar to protein kinase C and casein kinase substrate more 91.05(n)
    95.49(a)
    535109  XM_615104.3  XP_615104.3 
    rat
    (Rattus norvegicus)
    Pacsin31   -- protein kinase C and casein kinase substrate in neurons more 87.03(n)
    93.63(a)
    311187  NM_001009966.2  NP_001009966.1 
    mouse
    (Mus musculus)
    Pacsin31, 5 25
    protein kinase C and casein kinase substrate in neurons more1, 5 87.58(n)1
    94.34(a)1
    807081  NM_028733.21  NP_083009.11 
     AF1498245  AF2425315  (see all 17)
    About this table        Species with no ortholog for PACSIN3

    ENSEMBL Gene Tree for PACSIN3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for PACSIN3 gene
    PACSIN22  PACSIN12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/30 NCBI SNPs in PACSIN3 are shown (see all 30 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 23)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs9017501,2
    A,C,F,H,O47166048(+) TCTGAA/GACCAA 1 -- ng317Minor allele frequency- G:0.29NA EU EA WA 676
    rs71066541,2
    C,F,H47160559(+) TTCGGG/ACCCAG 1 V/A mis1 ese39Minor allele frequency- A:0.12EU EA WA NA 896
    rs47529701,2
    A47165558(+) CCCGGA/GCGCAG 1 -- ng310--------
    rs9017491,2
    A47165174(+) CTCCCC/GCTCCG 1 -- ng310--------
    rs22914451,2
    C,F,H47158696(-) GTGCGC/TGCCTG 1 R/R syn1 ese37Minor allele frequency- T:0.05NA EU EA WA 554
    rs108386641,2
    A,C,F,H47160480(+) GGCCCG/AACAGT 1 -- int14Minor allele frequency- A:0.34EU EA WA 412
    rs22794381,2
    A,C,F,H47160751(-) GCCCAT/ACTGAC 1 -- int112Minor allele frequency- A:0.45EA NA EU WA 2410
    rs110391191,2
    A,C,F,H47158500(+) CCCTGG/AATGCC 1 -- int14Minor allele frequency- A:0.43EU EA WA 404
    rs79374101,2
    A,C,F,H47162658(+) GAAAAC/GAAGCG 1 -- int1 trp312Minor allele frequency- G:0.38EU EA WA NA 1198
    rs79251371,2
    A,C,F,H47159522(+) CCATGC/ATCCCa 1 -- int14Minor allele frequency- A:0.29EU EA WA 406
    About this table

    HapMap Linkage Disequilibrium images for PACSIN3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 606513

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/22 PubMed articles for PACSIN3 gene (see all 22 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 29763 HGNC: 8572 AceView: PACSIN3andZNF289 Ensembl:ENSG00000165912 euGenes: HUgn29763
    ECgene: PACSIN3 H-InvDB: PACSIN3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for PACSIN3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for PACSIN3 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for PACSIN3 Browse small molecules at Sigma-Aldrich
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     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for PACSIN3




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for PACSIN3

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