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PABPC3 Gene

protein-coding   GIFtS: 53

GC13P024568
poly(A) binding protein, cytoplasmic 3
(Previous names: poly(A)-binding protein, cytoplasmic 3 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: PABPL3)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
PABP3 1, 2, 3, 5
PABPL3 2, 3, 5
Descriptions
PABP 3 3
Poly(A)-binding protein 3 3
Testis-specific poly(A)-binding protein 3
poly(A) binding protein, cytoplasmic 3 2
poly(A)-binding protein, cytoplasmic 3 1
poly(A)-binding protein-like 3 2
testis-specific poly(A)-binding protein (PABP) 2
testis-specific poly(A)-binding protein 3 2
External Ids
HGNC: 85561
Entrez Gene: 50422
UniProtKB: Q9H3613
Ensembl: ENSG000001518467
Search outside databases for aliases for PABPC3 gene

Previous GC identifers: GC13P023650 GC13P019650 GC13P024600 GC13P023468

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for PABPC3:
Messenger RNA stability and translation initiation are extensively under the control of
poly(A)-binding proteins (PABP). See PABPC1 (MIM 604679) for background information.[supplied by
OMIM]

UniProtKB/Swiss-Prot: PABP3_HUMAN, Q9H361
Function: Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of
mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1

Gene Wiki entry for PABPC3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the PABPC3 gene  

Entrez Gene cytogenetic band: 13q12-q13   Ensembl cytogenetic band:  13q12.13   HGNC cytogenetic band: 13q12-q13

PABPC3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13P024568:     (about GC identifiers)

Start:
24,568,276 bp from pter
End:
24,570,705 bp from pter
Size:
2,430 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000013.9  NT_024524.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: PABP3_HUMAN, Q9H361 (See protein sequence)
Recommended Name: Polyadenylate-binding protein 3  
Size: 631 amino acids; 70031 Da
Subcellular location: Cytoplasm (By similarity)
PDB structures from and Proteopedia :
2D9P (3D)    
Secondary accessions: Q8NHV0 Q9H086

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_112241.2  

    ENSEMBL proteins: 
    ENSP00000281589 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (PABPC3)
    Human Recombinant Proteins from Abnova (PABPC3)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm NAS11328870
    About this table

    Antibodies for PABPC3: 
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    Antibodies from Abcam (PABPC3), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (PABPC3)
    Novus Biologicals Antibodies for PABPC3

    Assays for PABPC3: 
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    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR002004 PABP_HYD
     IPR000504 RRM_RNP1
     IPR006515 PABP_1234
     IPR012677 a_b_plait_nuc_bd


       GeneDecks  PABPC3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9H361

    ProtoNet protein and cluster: Q9H361

    2 Blocks protein families:
    IPB000504 RNA-binding region RNP-1 (RNA recognition motif)
    IPB002004 Polyadenylate-binding protein/HECT-associated


    UniProtKB/Swiss-Prot: PABP3_HUMAN, Q9H361
    Similarity: Belongs to the polyadenylate-binding protein type-1 family
    Similarity: Contains 1 PABC domain
    Similarity: Contains 4 RRM (RNA recognition motif) domains

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PABPC3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (PABPC3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030979

                  Applied Biosystems Silencer® siRNAs for PABPC3

                  Sigma-Aldrich siRNA for PABPC3  
                         Sigma-Aldrich shRNA for PABPC3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for PABPC3
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030979
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030979
                                     untagged cDNA clone in CMV expression vector: NM_030979 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_030979

    UniProtKB/Swiss-Prot: PABP3_HUMAN, Q9H361
    Function: Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of
    mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1

    Genatlas biochemistry entry for PABPC3:
    poly(A) binding protein,cytoplasmic 3

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003723 RNA binding IEA--
    GO:0008143 poly(A) binding IDA11328870
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/234 Interacting proteins for PABPC3 (Q9H3612) via UniProtKB, MINT, and/or STRING (see all 234 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ATAD3BQ5T9A42MINT-6488791
    COPAP536212MINT-6488791
    DDX18Q9NVP12MINT-6488791
    DDX39O001482MINT-6488791
    DDX47Q9H0S42MINT-6488791
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016071 mRNA metabolic process NAS11328870
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for PABPC3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (PABPC3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (PABPC3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030979

                  Sigma-Aldrich siRNA for PABPC3  
                         Sigma-Aldrich shRNA for PABPC3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_030979  

    REFSEQ mRNAs for PABPC3 gene: 

    NM_030979.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_030979  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030979
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030979
                                     untagged cDNA clone in CMV expression vector: NM_030979 

    Additional cDNA sequence: 

    AF132026.1 AK313336.1 AL136900.1 BC027617.2 BC045608.1 

    24/30 DOTS entries (see all 30 ):

    DT.91661782  DT.95267608  DT.100700595  DT.92470838  DT.91954612  DT.92470896  DT.100700601  DT.91870787 
    DT.121466365  DT.92470903  DT.95267689  DT.100700590  DT.100700596  DT.120762244  DT.121466486  DT.91801712 
    DT.92359426  DT.95267669  DT.95267672  DT.100060609  DT.120762238  DT.120762240  DT.121466401  DT.121466465 

    24/32 AceView cDNA sequences (see all 32 ):

    AL040749 BC027617 AA954581 AL136900 CD243801 NM_030979 BI460001 AF132026 
    BG717008 AL040794 BU567424 BI825788 CD358574 AL040750 BG718483 AI025825 
    BI464014 CD358948 AL046737 BG723981 AA382298 CD358457 BE091857 BF349176 

    highest scoring ESTs for PABPC3:

    AA954581 AF132026 AI025825 AL040750 AL040794 AL136900 BC027617 BG717008 BG718483 BG723981 

    Unigene Cluster for PABPC3:

    Poly(A) binding protein, cytoplasmic 3
    Hs.458280  [show with all ESTs]
    Unigene Representative Sequence: BC045608


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000281589  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    PABPC3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for PABPC3

    1 / 2 / 3

    3 probe-sets matching PABPC3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    31951_s_at2, 3 U95-A 7 0.81 0.23 1.00 1.00 Z48501 0.20 0.07 0.15 2

    208113_x_at2, 3 U133-A 6 1.00 0.43 -- -- NM_030979 0.60 1.00 0.82 1

    208113_x_at2 U133Plus2 6 1.00 0.43 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TTGTTGGAGA

    SOURCE GeneReport for Unigene cluster: Hs.458280

    UniProtKB/Swiss-Prot: PABP3_HUMAN, Q9H361
    Tissue specificity: Testis specific

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for PABPC3 gene from 3 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    PABPC31   -- poly(A) binding protein, cytoplasmic 3 99(n)
    97.78(a)
    452494  XM_001154541.1  XP_001154541.1 
    fruit fly
    (Drosophila melanogaster)
    pAbp3 55B8--9 RNA binding 54(a)
    (best of 2)
      --
    worm
    (Caenorhabditis elegans)
    pab-23 X(13557928-13561043) RNA recognition motif. (aka RRM, RBD,
    or RNP domain) more
    54(a)
    (best of 2)
      --
    About this table        Species with no ortholog for PABPC3

    ENSEMBL Gene Tree for PABPC3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for PABPC3 gene
    PABPC1L2  PABPC52  PABPC1L2B2  PABPCP52  PABPCP22  PABPC42  PABPC1L2A2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/22 NCBI SNPs in PABPC3 are shown (see all 22 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 25)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 13 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs30022111,2
    A,C,F,H24567061(+) CCCCCC/AAACTG 1 -- ng518Minor allele frequency- A:0.28EU EA WA NA 780
    --
    rs24611,2
    C,H24568988(+) CTGCCT/GTAAGT 1 V/L mis16Minor allele frequency- G:0.20EU EA WA MN 774
    --
    rs95511771,2
    A,C24566725(+) ctcacG/Tcctgt 1 -- ng510--------
    rs30022121,2
    A,C,F,H24568813(+) ATGAAC/TGGAAT 1 N/N syn14Minor allele frequency- T:0.04EU EA WA 420
    --
    rs116197911,2
    F,H24568984(+) GGGCCC/TGCCTT 1 P/P syn1 ese34Minor allele frequency- T:0.02EU EA WA 414
    --
    rs95812591,2
    --24570895(+) AACATT/ATGTTC 1 -- ng3112Minor allele frequency- A:0.25EU EA WA 1256
    --
    rs581740401,2
    --24569352(+) TGTATA/GTTTCT 1 C/Y mis10--------
    --
    rs360344261,2
    --24566852(+) GGCGT-/GGGTAG 1 -- ng510--------
    --
    rs95535341,2
    --24570329(+) TAAAAA/TAAAAA 1 -- ut310--------
    --
    rs734753981,2
    --24567645(+) TTTTCA/GGGGTT 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for PABPC3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 604680

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    8 PubMed articles for PABPC3 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 5042 HGNC: 8556 AceView: PABPC3 Ensembl:ENSG00000151846 euGenes: HUgn5042
    ECgene: PABPC3 H-InvDB: PABPC3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for PABPC3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for PABPC3 Antibodies for PABPC3
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     Antibodies for PABPC3

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