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Aliases for P4HA3 Gene

Aliases for P4HA3 Gene

  • Prolyl 4-Hydroxylase, Alpha Polypeptide III 2 3
  • EC 4 63
  • Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Alpha Polypeptide III 2
  • Procollagen-Proline,2-Oxoglutarate-4-Dioxygenase Subunit Alpha-3 4
  • Collagen Prolyl 4-Hydroxylase Alpha(III) 2
  • 4-PH Alpha-3 4

External Ids for P4HA3 Gene

Previous GeneCards Identifiers for P4HA3 Gene

  • GC11M073705
  • GC11M073655
  • GC11M073977
  • GC11M070272
  • GC11M073947

Summaries for P4HA3 Gene

Entrez Gene Summary for P4HA3 Gene

  • This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

GeneCards Summary for P4HA3 Gene

P4HA3 (Prolyl 4-Hydroxylase, Alpha Polypeptide III) is a Protein Coding gene. Among its related pathways are Arginine and proline metabolism and Metabolism. GO annotations related to this gene include oxidoreductase activity and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is P4HA2.

UniProtKB/Swiss-Prot for P4HA3 Gene

  • Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for P4HA3 Gene

Genomics for P4HA3 Gene

Regulatory Elements for P4HA3 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for P4HA3 Gene

74,235,801 bp from pter
74,311,676 bp from pter
75,876 bases
Minus strand

Genomic View for P4HA3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for P4HA3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for P4HA3 Gene

Proteins for P4HA3 Gene

  • Protein details for P4HA3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Prolyl 4-hydroxylase subunit alpha-3
    Protein Accession:
    Secondary Accessions:
    • A0AV13
    • B4DUD3
    • Q5EBL3
    • Q5JPA9

    Protein attributes for P4HA3 Gene

    544 amino acids
    Molecular mass:
    61126 Da
    Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) ion per subunit.;
    Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
    Quaternary structure:
    • Heterotetramer of two alpha-3 chains and two beta chains (the beta chain is the multi-functional PDI).

    Alternative splice isoforms for P4HA3 Gene


neXtProt entry for P4HA3 Gene

Proteomics data for P4HA3 Gene at MOPED

Post-translational modifications for P4HA3 Gene

  • N-glycosylation plays no role in the catalytic activity
  • Glycosylation at Asn 242 and Asn 482
  • Modification sites at PhosphoSitePlus

Other Protein References for P4HA3 Gene

Antibody Products

  • Santa Cruz Biotechnology (SCBT) Antibodies for P4HA3

Domains & Families for P4HA3 Gene

Graphical View of Domain Structure for InterPro Entry



  • Contains 1 Fe2OG dioxygenase domain.
  • Belongs to the P4HA family.
  • Contains 1 TPR repeat.
  • Contains 1 Fe2OG dioxygenase domain.
  • Belongs to the P4HA family.
  • Contains 1 TPR repeat.
genes like me logo Genes that share domains with P4HA3: view

No data available for Gene Families for P4HA3 Gene

Function for P4HA3 Gene

Molecular function for P4HA3 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
L-proline-[procollagen] + 2-oxoglutarate + O(2) = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO(2)
UniProtKB/Swiss-Prot Function:
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Enzyme Numbers (IUBMB) for P4HA3 Gene

Gene Ontology (GO) - Molecular Function for P4HA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004656 procollagen-proline 4-dioxygenase activity IEA --
GO:0005506 iron ion binding IEA --
GO:0005515 protein binding IPI 25416956
GO:0016491 oxidoreductase activity --
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen IEA --
genes like me logo Genes that share ontologies with P4HA3: view
genes like me logo Genes that share phenotypes with P4HA3: view

Animal Model Products

miRNA for P4HA3 Gene

miRTarBase miRNAs that target P4HA3

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for P4HA3

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targets and HOMER Transcription for P4HA3 Gene

Localization for P4HA3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for P4HA3 Gene

Endoplasmic reticulum lumen.

Subcellular locations from

Jensen Localization Image for P4HA3 Gene COMPARTMENTS Subcellular localization image for P4HA3 gene
Compartment Confidence
endoplasmic reticulum 3

Gene Ontology (GO) - Cellular Components for P4HA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum --
GO:0005788 endoplasmic reticulum lumen IEA --
genes like me logo Genes that share ontologies with P4HA3: view

Pathways & Interactions for P4HA3 Gene

genes like me logo Genes that share pathways with P4HA3: view

Pathways by source for P4HA3 Gene

2 KEGG pathways for P4HA3 Gene

Gene Ontology (GO) - Biological Process for P4HA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0019511 peptidyl-proline hydroxylation IEA --
GO:0055114 oxidation-reduction process IEA --
genes like me logo Genes that share ontologies with P4HA3: view

No data available for SIGNOR curated interactions for P4HA3 Gene

Drugs & Compounds for P4HA3 Gene

(5) Drugs for P4HA3 Gene - From: HMDB and ApexBio

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
hydroxyproline Approved, Experimental Pharma 0
L-Proline Approved Nutra 0
Succinic acid Approved Nutra Full agonist, Agonist 0
Vitamin C Approved Nutra 398,398
DMOG Pharma Competitive HIF-PH inhibitor, cell-permeable 0

(4) Additional Compounds for P4HA3 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
carbon dioxide
  • Carbon oxide
  • Carbon-12 dioxide
  • Carbonic acid anhydride
  • Carbonic acid gas
  • Carbonic anhydride
  • Armco iron
  • Carbonyl iron
  • FE
  • Ferrovac e
  • Hematite
Oxoglutaric acid
  • 2-Ketoglutarate
  • 2-Ketoglutaric acid
  • 2-Oxo-1,5-pentanedioate
  • 2-Oxo-1,5-pentanedioic acid
  • 2-Oxoglutarate
  • Dioxygen
  • Molecular oxygen
  • O2
  • Oxygen
  • Oxygen molecule
genes like me logo Genes that share compounds with P4HA3: view

Transcripts for P4HA3 Gene

Unigene Clusters for P4HA3 Gene

Prolyl 4-hydroxylase, alpha polypeptide III:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for P4HA3

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for P4HA3 Gene

No ASD Table

Relevant External Links for P4HA3 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for P4HA3 Gene

mRNA expression in normal human tissues for P4HA3 Gene

mRNA differential expression in normal tissues according to GTEx for P4HA3 Gene

This gene is overexpressed in Prostate (x5.9) and Artery - Coronary (x5.6).

Protein differential expression in normal tissues from HIPED for P4HA3 Gene

This gene is overexpressed in Cerebrospinal fluid (66.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for P4HA3 Gene

SOURCE GeneReport for Unigene cluster for P4HA3 Gene Hs.660541

mRNA Expression by UniProt/SwissProt for P4HA3 Gene

Tissue specificity: Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.
genes like me logo Genes that share expression patterns with P4HA3: view

Protein tissue co-expression partners for P4HA3 Gene

- Elite partner

Primer Products

In Situ Assay Products

Orthologs for P4HA3 Gene

This gene was present in the common ancestor of animals.

Orthologs for P4HA3 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia P4HA3 35
  • 90.07 (n)
  • 91.54 (a)
P4HA3 36
  • 92 (a)
(Canis familiaris)
Mammalia P4HA3 35
  • 90.87 (n)
  • 93.01 (a)
P4HA3 36
  • 93 (a)
(Mus musculus)
Mammalia P4ha3 35
  • 87.27 (n)
  • 89.3 (a)
P4ha3 16
P4ha3 36
  • 90 (a)
(Pan troglodytes)
Mammalia P4HA3 35
  • 99.88 (n)
  • 100 (a)
P4HA3 36
  • 84 (a)
(Rattus norvegicus)
Mammalia P4ha3 35
  • 87.07 (n)
  • 88.42 (a)
(Monodelphis domestica)
Mammalia P4HA3 36
  • 80 (a)
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 15 (a)
(Gallus gallus)
Aves P4HA3 35
  • 69.14 (n)
  • 68.95 (a)
P4HA3 36
  • 67 (a)
(Anolis carolinensis)
Reptilia P4HA3 36
  • 68 (a)
(Danio rerio)
Actinopterygii p4ha3 35
  • 62.05 (n)
  • 59.84 (a)
p4ha3 36
  • 53 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG11828 36
  • 24 (a)
CG15539 36
  • 27 (a)
CG15864 36
  • 24 (a)
CG18231 36
  • 22 (a)
CG18233 36
  • 22 (a)
CG18234 36
  • 23 (a)
CG18749 36
  • 26 (a)
CG31013 36
  • 27 (a)
CG31016 36
  • 25 (a)
CG31371 36
  • 24 (a)
CG31524 36
  • 24 (a)
CG32199 36
  • 24 (a)
CG32201 36
  • 23 (a)
CG34345 36
  • 24 (a)
CG4174 36
  • 22 (a)
CG9698 36
  • 27 (a)
PH4alphaMP 36
  • 30 (a)
PH4alphaNE1 36
  • 31 (a)
PH4alphaNE2 36
  • 25 (a)
PH4alphaNE3 36
  • 25 (a)
PH4alphaPV 36
  • 28 (a)
PH4alphaSG1 36
  • 33 (a)
PH4alphaSG2 36
  • 30 (a)
(Caenorhabditis elegans)
Secernentea C14E2.4 36
  • 19 (a)
phy-3 36
  • 19 (a)
phy-4 36
  • 23 (a)
Y43F8B.19 36
  • 20 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.423 36
  • 16 (a)
Species with no ortholog for P4HA3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for P4HA3 Gene

Gene Tree for P4HA3 (if available)
Gene Tree for P4HA3 (if available)

Paralogs for P4HA3 Gene

Paralogs for P4HA3 Gene

(3) SIMAP similar genes for P4HA3 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with P4HA3: view

Variants for P4HA3 Gene

Sequence variations from dbSNP and Humsavar for P4HA3 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs471539 -- 74,260,148(-) TCCCT(C/T)CACAT intron-variant
rs474280 -- 74,297,818(-) AATTC(A/G)TCACA intron-variant
rs475326 -- 74,259,708(-) CTAAA(C/T)GAAAT intron-variant
rs486577 -- 74,278,353(-) TTCTA(C/T)GTCAT intron-variant
rs488893 -- 74,282,942(+) GCCCA(A/G)CGAAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for P4HA3 Gene

Variant ID Type Subtype PubMed ID
dgv397e1 CNV Complex 17122850
esv34979 CNV Gain 17911159
nsv825994 CNV Gain 20364138
esv267677 CNV Insertion 20981092

Variation tolerance for P4HA3 Gene

Residual Variation Intolerance Score: 69.57% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 10.67; 91.42% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for P4HA3 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for P4HA3 Gene

Disorders for P4HA3 Gene

Relevant External Links for P4HA3

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with P4HA3: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for P4HA3 Gene

Publications for P4HA3 Gene

  1. Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme. (PMID: 14500733) Kukkola L. … Myllyharju J. (J. Biol. Chem. 2003) 2 67
  2. A proteome-scale map of the human interactome network. (PMID: 25416956) Rolland T. … Vidal M. (Cell 2014) 67
  3. Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism. (PMID: 24722188) Corominas R. … Iakoucheva L.M. (Nat. Commun. 2014) 67
  4. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. (PMID: 22658674) Castello A. … Hentze M.W. (Cell 2012) 67
  5. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose J.E. … Uhl G.R. (Mol. Med. 2010) 67

Products for P4HA3 Gene

Sources for P4HA3 Gene

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