Free for academic non-profit institutions. Other users need a Commercial license

Aliases for P4HA3 Gene

Aliases for P4HA3 Gene

  • Prolyl 4-Hydroxylase Subunit Alpha 3 2 3 5
  • EC 1.14.11.2 4 61
  • Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Alpha Polypeptide III 2
  • Procollagen-Proline,2-Oxoglutarate-4-Dioxygenase Subunit Alpha-3 4
  • Prolyl 4-Hydroxylase, Alpha Polypeptide III 2
  • Collagen Prolyl 4-Hydroxylase Alpha(III) 2
  • 4-PH Alpha-3 4

External Ids for P4HA3 Gene

Previous GeneCards Identifiers for P4HA3 Gene

  • GC11M073705
  • GC11M073655
  • GC11M073977
  • GC11M070272
  • GC11M073947

Summaries for P4HA3 Gene

Entrez Gene Summary for P4HA3 Gene

  • This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

GeneCards Summary for P4HA3 Gene

P4HA3 (Prolyl 4-Hydroxylase Subunit Alpha 3) is a Protein Coding gene. Among its related pathways are Collagen chain trimerization and Arginine and proline metabolism. GO annotations related to this gene include oxidoreductase activity and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is P4HA2.

UniProtKB/Swiss-Prot for P4HA3 Gene

  • Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for P4HA3 Gene

Genomics for P4HA3 Gene

Regulatory Elements for P4HA3 Gene

Enhancers for P4HA3 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH11G074276 1.1 Ensembl ENCODE 37.4 +33.0 32960 4.1 SIN3A RAD21 FOS RELB IKZF2 CBFB REST TBX21 IKZF1 PBX2 P4HA3 ENSG00000206913 P4HA3-AS1 KCNE3 ENSG00000254928 PPME1 PGM2L1 PIR36741 GC11P074254
GH11G074133 1.5 Ensembl ENCODE dbSUPER 20.7 +173.4 173419 10.2 FOXA2 PKNOX1 ARID4B BRCA1 TCF12 GLIS2 FOS DEK ZNF263 REST PPME1 P4HA3 C2CD3 UCP3 UCP2 ENSG00000206913 ENSG00000256098
GH11G074017 1.8 FANTOM5 Ensembl ENCODE dbSUPER 16.7 +291.1 291053 6.1 PKNOX1 FOXA2 ARID4B YY1 TCF12 ZNF207 FOS PPARG TBX21 KAT8 P4HA3 PPME1 PAAF1 FAM168A ENSG00000254631 UCP3 ENSG00000256098
GH11G074024 1.6 FANTOM5 Ensembl ENCODE dbSUPER 18.4 +285.9 285904 3.0 HDAC1 NFIB ZNF175 ZNF384 CEBPG FOSL1 ZNF121 FOS SMARCE1 CEBPB P4HA3 ENSG00000255440 UCP3 ENSG00000256098
GH11G074169 1.4 ENCODE dbSUPER 20.5 +140.2 140217 3.5 HDGF PKNOX1 FOXA2 MLX CREB3L1 ARNT ARID4B SIN3A YBX1 DMAP1 P4HA3 ENSG00000206913 PPME1 POLD3 RNF169 PAAF1 RPS12P22 C2CD3 GC11P074171
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around P4HA3 on UCSC Golden Path with GeneCards custom track

Promoters for P4HA3 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000041975 76 401 CTCF SP3 RB1 L3MBTL2 NRF1 ZNF10 UBTF REST RAD21 E2F1

Genomic Location for P4HA3 Gene

Chromosome:
11
Start:
74,235,801 bp from pter
End:
74,311,676 bp from pter
Size:
75,876 bases
Orientation:
Minus strand

Genomic View for P4HA3 Gene

Genes around P4HA3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
P4HA3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for P4HA3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for P4HA3 Gene

Proteins for P4HA3 Gene

  • Protein details for P4HA3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q7Z4N8-P4HA3_HUMAN
    Recommended name:
    Prolyl 4-hydroxylase subunit alpha-3
    Protein Accession:
    Q7Z4N8
    Secondary Accessions:
    • A0AV13
    • B4DUD3
    • Q5EBL3
    • Q5JPA9

    Protein attributes for P4HA3 Gene

    Size:
    544 amino acids
    Molecular mass:
    61126 Da
    Cofactor:
    Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
    Cofactor:
    Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
    Quaternary structure:
    • Heterotetramer of two alpha-3 chains and two beta chains (the beta chain is the multi-functional PDI).

    Alternative splice isoforms for P4HA3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for P4HA3 Gene

Selected DME Specific Peptides for P4HA3 Gene

Q7Z4N8:
  • NAALFWWNLHRSGEGD
  • ARNVLKYE
  • YRISKSAWL
  • LAEPWLQRSVVASGEKQL
  • GNRVATF
  • NYGIGGHY
  • GQEFRRPC
  • PHLQTRDTYEGLCQTLGSQPT
  • YMLNVKGLA
  • IPSLYCSYET
  • LYHDFVSD

Post-translational modifications for P4HA3 Gene

  • N-glycosylation plays no role in the catalytic activity.
  • Glycosylation at Asn242 and posLast=482482
  • Modification sites at PhosphoSitePlus

Other Protein References for P4HA3 Gene

Domains & Families for P4HA3 Gene

Graphical View of Domain Structure for InterPro Entry

Q7Z4N8

UniProtKB/Swiss-Prot:

P4HA3_HUMAN :
  • Belongs to the P4HA family.
Family:
  • Belongs to the P4HA family.
genes like me logo Genes that share domains with P4HA3: view

No data available for Gene Families for P4HA3 Gene

Function for P4HA3 Gene

Molecular function for P4HA3 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
L-proline-[procollagen] + 2-oxoglutarate + O(2) = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO(2).
UniProtKB/Swiss-Prot Function:
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Enzyme Numbers (IUBMB) for P4HA3 Gene

Gene Ontology (GO) - Molecular Function for P4HA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004656 procollagen-proline 4-dioxygenase activity IEA --
GO:0005506 iron ion binding IEA --
GO:0005515 protein binding IPI 25416956
GO:0016491 oxidoreductase activity IEA --
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen IEA --
genes like me logo Genes that share ontologies with P4HA3: view
genes like me logo Genes that share phenotypes with P4HA3: view

Animal Model Products

  • Taconic Biosciences Mouse Models for P4HA3

CRISPR Products

miRNA for P4HA3 Gene

miRTarBase miRNAs that target P4HA3

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for P4HA3 Gene

Localization for P4HA3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for P4HA3 Gene

Endoplasmic reticulum lumen.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for P4HA3 gene
Compartment Confidence
endoplasmic reticulum 5
extracellular 3
mitochondrion 1
lysosome 1

Gene Ontology (GO) - Cellular Components for P4HA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum IEA --
GO:0005788 endoplasmic reticulum lumen TAS --
genes like me logo Genes that share ontologies with P4HA3: view

Pathways & Interactions for P4HA3 Gene

genes like me logo Genes that share pathways with P4HA3: view

Pathways by source for P4HA3 Gene

Gene Ontology (GO) - Biological Process for P4HA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0019511 peptidyl-proline hydroxylation IEA --
GO:0055114 oxidation-reduction process IEA --
genes like me logo Genes that share ontologies with P4HA3: view

No data available for SIGNOR curated interactions for P4HA3 Gene

Drugs & Compounds for P4HA3 Gene

(7) Drugs for P4HA3 Gene - From: ApexBio and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Carbon dioxide Approved, Vet_approved Pharma 0
hydroxyproline Approved, Experimental Pharma 0
Oxygen Approved, Vet_approved Pharma 0
L-Proline Approved Nutra 0
Succinic acid Approved Nutra Full agonist, Agonist 0

(2) Additional Compounds for P4HA3 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Fe2+
  • Armco iron
  • Carbonyl iron
  • FE
  • Ferrovac e
  • Hematite
15438-31-0
Oxoglutaric acid
  • 2-Ketoglutarate
  • 2-Ketoglutaric acid
  • 2-Oxo-1,5-pentanedioate
  • 2-Oxo-1,5-pentanedioic acid
  • 2-Oxoglutarate
328-50-7

(1) ApexBio Compounds for P4HA3 Gene

Compound Action Cas Number
DMOG Competitive HIF-PH inhibitor, cell-permeable 89464-63-1
genes like me logo Genes that share compounds with P4HA3: view

Transcripts for P4HA3 Gene

Unigene Clusters for P4HA3 Gene

Prolyl 4-hydroxylase, alpha polypeptide III:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for P4HA3 Gene

No ASD Table

Relevant External Links for P4HA3 Gene

GeneLoc Exon Structure for
P4HA3
ECgene alternative splicing isoforms for
P4HA3

Expression for P4HA3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for P4HA3 Gene

mRNA differential expression in normal tissues according to GTEx for P4HA3 Gene

This gene is overexpressed in Prostate (x5.9) and Artery - Coronary (x5.6).

Protein differential expression in normal tissues from HIPED for P4HA3 Gene

This gene is overexpressed in Cerebrospinal fluid (66.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for P4HA3 Gene



Protein tissue co-expression partners for P4HA3 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of P4HA3 Gene:

P4HA3

SOURCE GeneReport for Unigene cluster for P4HA3 Gene:

Hs.660541

mRNA Expression by UniProt/SwissProt for P4HA3 Gene:

Q7Z4N8-P4HA3_HUMAN
Tissue specificity: Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.

Evidence on tissue expression from TISSUES for P4HA3 Gene

  • Heart(4.2)
  • Muscle(4.2)
  • Intestine(4.1)
genes like me logo Genes that share expression patterns with P4HA3: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for P4HA3 Gene

Orthologs for P4HA3 Gene

This gene was present in the common ancestor of animals.

Orthologs for P4HA3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia P4HA3 34 35
  • 99.88 (n)
dog
(Canis familiaris)
Mammalia P4HA3 34 35
  • 90.87 (n)
cow
(Bos Taurus)
Mammalia P4HA3 34 35
  • 90.07 (n)
mouse
(Mus musculus)
Mammalia P4ha3 34 16 35
  • 87.27 (n)
rat
(Rattus norvegicus)
Mammalia P4ha3 34
  • 87.07 (n)
oppossum
(Monodelphis domestica)
Mammalia P4HA3 35
  • 80 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 15 (a)
ManyToMany
chicken
(Gallus gallus)
Aves P4HA3 34 35
  • 69.14 (n)
lizard
(Anolis carolinensis)
Reptilia P4HA3 35
  • 68 (a)
OneToOne
zebrafish
(Danio rerio)
Actinopterygii p4ha3 34 35
  • 62.05 (n)
fruit fly
(Drosophila melanogaster)
Insecta PH4alphaSG1 35
  • 33 (a)
ManyToMany
PH4alphaNE1 35
  • 31 (a)
ManyToMany
PH4alphaMP 35
  • 30 (a)
ManyToMany
PH4alphaSG2 35
  • 30 (a)
ManyToMany
PH4alphaPV 35
  • 28 (a)
ManyToMany
CG15539 35
  • 27 (a)
ManyToMany
CG31013 35
  • 27 (a)
ManyToMany
CG9698 35
  • 27 (a)
ManyToMany
CG18749 35
  • 26 (a)
ManyToMany
CG31016 35
  • 25 (a)
ManyToMany
PH4alphaNE2 35
  • 25 (a)
ManyToMany
PH4alphaNE3 35
  • 25 (a)
ManyToMany
CG15864 35
  • 24 (a)
ManyToMany
CG32199 35
  • 24 (a)
ManyToMany
CG34345 35
  • 24 (a)
ManyToMany
CG31524 35
  • 24 (a)
ManyToMany
CG31371 35
  • 24 (a)
ManyToMany
CG11828 35
  • 24 (a)
ManyToMany
CG18234 35
  • 23 (a)
ManyToMany
CG32201 35
  • 23 (a)
ManyToMany
CG18231 35
  • 22 (a)
ManyToMany
CG18233 35
  • 22 (a)
ManyToMany
CG4174 35
  • 22 (a)
ManyToMany
worm
(Caenorhabditis elegans)
Secernentea phy-4 35
  • 23 (a)
ManyToMany
Y43F8B.19 35
  • 20 (a)
ManyToMany
C14E2.4 35
  • 19 (a)
ManyToMany
phy-3 35
  • 19 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.423 35
  • 16 (a)
ManyToMany
Species where no ortholog for P4HA3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for P4HA3 Gene

ENSEMBL:
Gene Tree for P4HA3 (if available)
TreeFam:
Gene Tree for P4HA3 (if available)

Paralogs for P4HA3 Gene

Paralogs for P4HA3 Gene

(3) SIMAP similar genes for P4HA3 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with P4HA3: view

Variants for P4HA3 Gene

Sequence variations from dbSNP and Humsavar for P4HA3 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type
rs1000007787 -- 74,260,391(+) GTTCC(C/T)CTTTT intron-variant
rs1000013050 -- 74,277,387(+) ACAAA(A/G)AGGAA intron-variant
rs1000173339 -- 74,282,235(+) TACCA(C/G)TCACT intron-variant
rs1000212695 -- 74,271,629(+) GTAGC(A/G)GGGAC intron-variant
rs1000287210 -- 74,306,770(+) AGGAG(A/C)CAAAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for P4HA3 Gene

Variant ID Type Subtype PubMed ID
dgv209e214 CNV gain 21293372
esv2759837 CNV gain 17122850
esv3303398 CNV mobile element insertion 20981092
esv3339852 CNV insertion 20981092
esv3364688 CNV insertion 20981092
esv34979 CNV gain 17911159
esv3579744 CNV loss 25503493
esv3626833 CNV gain 21293372
esv3626835 CNV gain 21293372
nsv825994 CNV gain 20364138

Variation tolerance for P4HA3 Gene

Residual Variation Intolerance Score: 69.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 10.67; 91.42% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for P4HA3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
P4HA3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for P4HA3 Gene

Disorders for P4HA3 Gene

Relevant External Links for P4HA3

Genetic Association Database (GAD)
P4HA3
Human Genome Epidemiology (HuGE) Navigator
P4HA3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
P4HA3

No disorders were found for P4HA3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for P4HA3 Gene

Publications for P4HA3 Gene

  1. Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme. (PMID: 14500733) Kukkola L. … Myllyharju J. (J. Biol. Chem. 2003) 2 3 4 64
  2. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose J.E. … Uhl G.R. (Mol. Med. 2010) 3 46 64
  3. Cloning of a novel prolyl 4-hydroxylase subunit expressed in the fibrous cap of human atherosclerotic plaque. (PMID: 12874193) Van Den Diepstraten C. … Pickering J.G. (Circulation 2003) 3 4 64
  4. Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism. (PMID: 24722188) Corominas R. … Iakoucheva L.M. (Nat. Commun. 2014) 3 64
  5. A proteome-scale map of the human interactome network. (PMID: 25416956) Rolland T. … Vidal M. (Cell 2014) 3 64

Products for P4HA3 Gene

Sources for P4HA3 Gene

Content
Loading form....