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P2RX3 Gene

protein-coding   GIFtS: 62

GC11P056862
purinergic receptor P2X, ligand-gated ion channel, 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
MGC129956 2
P2X3 1, 2, 3, 5
Descriptions
ATP receptor 2, 3
P2X purinoceptor 3 2
P2X receptor, subunit 3 2
Purinergic receptor 3
purinergic receptor P2X, ligand-gated ion channel, 3 2
purinergic receptor P2X3 2
purinoceptor P2X3 2
External Ids
HGNC: 85341
Entrez Gene: 50242
UniProtKB: P563733
Ensembl: ENSG000001099917
Search outside databases for aliases for P2RX3 gene

Previous GC identifers: GC11P059499 GC11P058618 GC11P057356 GC11P056881

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for P2RX3:
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as
a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies
suggest that this receptor is important for peripheral pain responses, and also participates in
pathways controlling urinary bladder volume reflexes. It is possible that the development of
selective antagonists for this receptor may have a therapeutic potential in pain relief and in the
treatment of disorders of urine storage. [provided by RefSeq]

UniProtKB/Swiss-Prot: P2RX3_HUMAN, P56373
Function: Receptor for ATP that acts as a ligand gated ion channel

Gene Wiki entry for P2RX3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the P2RX3 gene  

Entrez Gene cytogenetic band: 11q12   Ensembl cytogenetic band:  11q12.1   HGNC cytogenetic band: 11q12

P2RX3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P056862:     (about GC identifiers)

Start:
56,862,525 bp from pter
End:
56,894,125 bp from pter
Size:
31,601 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000011.8  NT_033903.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: P2RX3_HUMAN, P56373 (See protein sequence)
Recommended Name: P2X purinoceptor 3  
Size: 397 amino acids; 44230 Da
Subunit: Homo- or heteropolymers (By similarity)
Subcellular location: Membrane; Multi-pass membrane protein
Secondary accessions: Q9UQB6

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_002550.2  

    ENSEMBL proteins: 
    ENSP00000263314 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Millipore Purified and/or Recombinant P2RX3 Protein
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (P2X3)
    Human Recombinant Proteins from Abnova (P2RX3)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005887 integral to plasma membrane TAS9221902
    GO:0016020 membrane IEA--
    GO:0030424 axon IEA--
    GO:0044424 intracellular part IEA--
    About this table

    Antibodies for P2RX3: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of P2RX3
    Sigma-Aldrich Antibodies for P2RX3
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (P2X3), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibodies for P2RX3

    Assays for P2RX3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR001429 P2X_purnocptor
     IPR003046 P2X3_purnocptor


       GeneDecks  P2RX3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P56373

    ProtoNet protein and cluster: P56373

    2 Blocks protein families:
    IPB001429 ATP P2X receptor
    IPB003046 P2X3 purinoceptor signature


    UniProtKB/Swiss-Prot: P2RX3_HUMAN, P56373
    Similarity: Belongs to the P2X receptor family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (P2RX3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (P2RX3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002559

                  Applied Biosystems Silencer® siRNAs for P2RX3

                  Sigma-Aldrich siRNA and siRNA Panels for P2RX3  
                         Sigma-Aldrich shRNA Panels and shRNA for P2RX3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002559
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002559
                                     untagged cDNA clone in CMV expression vector: NM_002559 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_002559

    UniProtKB/Swiss-Prot: P2RX3_HUMAN, P56373
    Function: Receptor for ATP that acts as a ligand gated ion channel

    Genatlas biochemistry entry for P2RX3:
    purinoceptor,P2X3,ionotropic class,expressed in heart and spinal cord,coexpressed with P2RX2 in
    sensory neurons,fibers,running into the spinal cord

    5 MGI mutant phenotypes (inferred from 2 alleles(MGI details for P2rx3):

    behavior/neurologicallethality-postnatalnervous systemnormaltouch/vibrissae

    5/7 Gene Ontology (GO) molecular function terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001614 purinergic nucleotide receptor activity NAS17895406
    GO:0004872 receptor activity IEA--
    GO:0004931 ATP-gated cation channel activity TAS9221902
    GO:0005216 ion channel activity IEA--
    GO:0005524 ATP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    3 Millipore Pathways for P2RX3
      inward current
      inward current
     Calcium channels

       GeneDecks  P2RX3 for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for P2RX3):
     hsa04020 Calcium signaling pathway
     hsa04080 Neuroactive ligand-receptor interaction

       GeneDecks  P2RX3 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  P2RX3 


    5/21 Interacting proteins for P2RX3 (ENSP000002633143) via UniProtKB, MINT, and/or STRING (see all 21 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    P2RX2ENSP000003433393STRING (score=.928)
    P2RY2ENSP000003103053STRING (score=.902)
    P2RX5ENSP000003712553STRING (score=.822)
    P2RX7ENSP000002618263STRING (score=.819)
    P2RX1ENSP000002255383STRING (score=.816)
    About this table

    5/16 Gene Ontology (GO) biological process terms (links to tree view) (see all 16 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001666 response to hypoxia IEA--
    GO:0006812 cation transport IEA--
    GO:0007165 signal transduction TAS9221902
    GO:0007274 neuromuscular synaptic transmission IEA--
    GO:0009408 response to heat IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Compounds for P2RX3 available from Tocris Bioscience
    CompoundAction CAS number
    RO-3Selective P2X2 and P2X2/3 antagonist
    NF 110Potent P2X3 antagonist[111150-22-2]
    NF 023Selective, competitive P2X antagonist[104869-31-0]
    2-Methylthioadenosine triphosphate tetrasodium saltP2 purinergic agonist[43170-89-4]
    SpinorphinPotent P2X3 antagonist[137201-62-8]
    About this table


    10 Novoseek chemical compound relationships for P2RX3 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    tnp-atp 77.56 2 11093790 (1), 10836147 (1)
    atp 74.89 33 9723962 (3), 14520770 (2), 16093386 (2), 17351651 (2) (see all 24)
    resiniferatoxin 65.03 3 15245821 (2)
    adpbetas 61.89 1 17351651 (1)
    2-methylthio-atp 61.00 1 15208093 (1)
    calcium 20.75 5 9723962 (3), 12482951 (1)
    histidine 6.11 1 9606184 (1)
    zinc 2.96 3 18242779 (1), 9151945 (1), 15993446 (1)
    nitric oxide 0.00 1 15621221 (1)
    ethanol 0.00 6 15993446 (3), 12694404 (1), 18639563 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (P2RX3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (P2RX3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002559

                  Sigma-Aldrich siRNA and siRNA Panels for P2RX3  
                         Sigma-Aldrich shRNA Panels and shRNA for P2RX3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_002559  

    REFSEQ mRNAs for P2RX3 gene: 

    NM_002559.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_002559  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002559
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002559
                                     untagged cDNA clone in CMV expression vector: NM_002559 

    Additional cDNA sequence: 

    AB016608.1 BC069162.1 BC074792.2 BC074793.2 BC109287.2 

    2 DOTS entries:

    DT.40126934  DT.99990187 

    10 AceView cDNA sequences:

    BC074793 BC074792 BC069162 BX116711 NM_002559 AI147403 BQ887878 AB016608 
    CD359708 Y07683 

    highest scoring ESTs for P2RX3:

    AB016608 BQ887878 Y07683 BC069162 BC074792 BC074793 NM_002559 AI147403 BC109287 BX116711 

    Unigene Cluster for P2RX3:

    Purinergic receptor P2X, ligand-gated ion channel, 3
    Hs.146738  [show with all ESTs]
    Unigene Representative Sequence: NM_002559


    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for P2RX3

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13
    SP1:              -                                   -                                             
    SP2:                                                                                                
    SP3:                                                                                                

    About this scheme

    ECgene alternative splicing isoforms for P2RX3

    1 Ensembl transcript including schematic representation:
    ENST00000263314  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    P2RX3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for P2RX3

    1 / 2 / 3

    3 probe-sets matching P2RX3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    31733_at2, 3 U95-A 2 1.00 0.97 1.00 1.00 Y07683 0.80 1.00 0.91 1

    208338_at2, 3 U133-A 1 1.00 1.00 -- -- NM_002559 0.60 1.00 0.82 1

    208338_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AAGACCTGCC

    SOURCE GeneReport for Unigene cluster: Hs.146738

    Expression variation in blood from EXPOLDB for P2RX3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for P2RX3 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    P2RX31   -- purinergic receptor P2X, ligand-gated ion channel, more 90.26(n)
    94.71(a)
    448782  XM_540614.1  XP_540614.1 
    chimpanzee
    (Pan troglodytes)
    P2RX31   -- purinergic receptor P2X, ligand-gated ion channel, more 99.58(n)
    99.75(a)
    742961  XM_001136930.1  XP_001136930.1 
    cow
    (Bos taurus)
    P2RX31   -- purinergic receptor P2X, ligand-gated ion channel, more 88.82(n)
    92.88(a)
    530468  XM_608941.3  XP_608941.3 
    rat
    (Rattus norvegicus)
    P2rx31   -- purinergic receptor P2X, ligand-gated ion channel, more 88.25(n)
    93.7(a)
    81739  NM_031075.1  NP_112337.1 
    mouse
    (Mus musculus)
    P2rx31, 5 25
    purinergic receptor P2X, ligand-gated ion channel, more1, 5 89(n)1
    93.7(a)1
    2281391  NM_145526.21  NP_663501.21 
     AK0530215  AK1402835  (see all 8)
    About this table        Species with no ortholog for P2RX3

    ENSEMBL Gene Tree for P2RX3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for P2RX3 gene
    P2RX22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/166 NCBI SNPs in P2RX3 are shown (see all 166 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 68)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs37818941,2
    A,C,F,H56894237(-) TGAGAT/CTGGGG 1 -- ng315Minor allele frequency- C:0.43EA EU WA 1530
    rs22760381,2
    C,F,H,O56894000(-) TCTCCA/GCTGTG 1 A/V mis1 ese313Minor allele frequency- N:0.00EA EU WA NA 2550
    --
    rs112290071,2
    H56873924(+) GGGCAG/CGATTT 1 H/Q mis1 ese34Minor allele frequency- C:0.00EU EA WA 366
    --
    rs37818931,2
    C56894410(-) aggatC/Tattct 1 -- ng310--------
    rs22760391,2
    A,C,F,H56893947(-) TTCAGC/TGTAGT 1 T/T syn112Minor allele frequency- T:0.47EA EU WA NA 2474
    rs112290051,2
    C,H56870720(+) GTGACG/ACCACC 1 T/T syn13Minor allele frequency- A:0.00EU EA 300
    rs8796461,2
    A,C,F,H,O56880772(+) ATATGC/TGTTAA 1 -- int116Minor allele frequency- N:0.00EA NA NS EU WA 878
    rs22760401,2
    A,C,F,H,O56893872(-) TGTGAT/CCCCAT 1 -- int18Minor allele frequency- C:0.49EA EU WA NA 2040
    rs22760421,2
    A,C,F,H56873961(-) GAATGA/GCTAGG 1 -- int15Minor allele frequency- G:0.45EA EU WA 1906
    rs37819021,2
    A,C,F,H56876623(-) GCTTTC/TCTGCC 1 -- int117Minor allele frequency- T:0.47EA EU WA NA 3082
    About this table

    HapMap Linkage Disequilibrium images for P2RX3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 600843

    3 Novoseek disease relationships for P2RX3 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    hyperalgesia 51.74 1 18042830 (1)
    overactive bladder 49.65 1 11412212 (1)
    inflammation 1.56 1 14650754 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/89 PubMed articles for P2RX3 gene (see all 89 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 5024 HGNC: 8534 AceView: P2RX3 Ensembl:ENSG00000109991 euGenes: HUgn5024
    ECgene: P2RX3 H-InvDB: P2RX3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    Wikipedia http://en.wikipedia.org/wiki/P2X_receptor
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for P2RX3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for P2RX3 Antibodies for P2RX3
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for P2RX3 Browse small molecules at Sigma-Aldrich
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     Antibodies for P2RX3

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