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Aliases for OTULIN Gene

Aliases for OTULIN Gene

  • OTU Deubiquitinase With Linear Linkage Specificity 2 3
  • FAM105B 3 4 6
  • OTU Domain-Containing Deubiquitinase With Linear Linkage Specificity 3 4
  • Family With Sequence Similarity 105, Member B 2 3
  • Deubiquitinating Enzyme Otulin 3 4
  • Ubiquitin Thioesterase Gumby 3 4
  • Gumby 2 3
  • GUM 3 6
  • Ubiquitin Thioesterase Otulin 3
  • Protein FAM105B 3
  • EC 3.4.19.12 4

External Ids for OTULIN Gene

Previous Symbols for OTULIN Gene

  • FAM105B

Summaries for OTULIN Gene

GeneCards Summary for OTULIN Gene

OTULIN (OTU Deubiquitinase With Linear Linkage Specificity) is a Protein Coding gene. An important paralog of this gene is FAM105A.

UniProtKB/Swiss-Prot for OTULIN Gene

  • Deubiquitinase that specifically removes linear (Met-1-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Associates with the LUBAC complex via direct interaction with RNF31 and counteracts its action by cleaving linear polyubiquitin chains to substrates. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by counteracting activity of the LUBAC complex. Plays a key role in innate immune response: required to restrict linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for OTULIN Gene

Genomics for OTULIN Gene

Genomic Location for OTULIN Gene

Start:
14,664,664 bp from pter
End:
14,699,733 bp from pter
Size:
35,070 bases
Orientation:
Plus strand

Genomic View for OTULIN Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for OTULIN Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for OTULIN Gene

No data available for Regulatory Elements for OTULIN Gene

Proteins for OTULIN Gene

  • Protein details for OTULIN Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96BN8-OTUL_HUMAN
    Recommended name:
    Ubiquitin thioesterase otulin
    Protein Accession:
    Q96BN8
    Secondary Accessions:
    • D3DTD3
    • Q8NAS0
    • Q96IA3

    Protein attributes for OTULIN Gene

    Size:
    352 amino acids
    Molecular mass:
    40263 Da
    Quaternary structure:
    • Interacts with RNF31; the interaction is direct.
    SequenceCaution:
    • Sequence=AAH07706.3; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH15392.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAC03828.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for OTULIN Gene

neXtProt entry for OTULIN Gene

Proteomics data for OTULIN Gene at MOPED

Post-translational modifications for OTULIN Gene

Other Protein References for OTULIN Gene

No data available for DME Specific Peptides for OTULIN Gene

Domains for OTULIN Gene

Gene Families for OTULIN Gene

HGNC:
  • OTUD :OTU domain containing

Protein Domains for OTULIN Gene

InterPro:
ProtoNet:

UniProtKB/Swiss-Prot:

OTUL_HUMAN
Domain:
  • The specificity for linear polyubiquitin is given by the Glu-16 residue in ubiquitin chain.:
    • Q96BN8
  • Contains 1 OTU domain.:
    • Q96BN8
Family:
  • Belongs to the peptidase C65 family. Otulin subfamily.:
    • Q96BN8
genes like me logo Genes that share domains with OTULIN: view

Function for OTULIN Gene

Molecular function for OTULIN Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=7.98 uM for linear diubiquitin {ECO:0000269 PubMed:23746843}; Note=Kcat is 6.3 sec(-1) with linear diubiquitin as substrate.;
UniProtKB/Swiss-Prot CatalyticActivity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
UniProtKB/Swiss-Prot Function: Deubiquitinase that specifically removes linear (Met-1-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Associates with the LUBAC complex via direct interaction with RNF31 and counteracts its action by cleaving linear polyubiquitin chains to substrates. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by counteracting activity of the LUBAC complex. Plays a key role in innate immune response: required to restrict linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling.

Enzyme Numbers (IUBMB) for OTULIN Gene

Gene Ontology (GO) - Molecular Function for OTULIN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004843 ubiquitin-specific protease activity IDA 23746843
GO:0005515 protein binding IPI 23708998
GO:0008234 cysteine-type peptidase activity IMP 23746843
genes like me logo Genes that share ontologies with OTULIN: view
genes like me logo Genes that share phenotypes with OTULIN: view

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for OTULIN Gene

Localization for OTULIN Gene

Subcellular locations from UniProtKB/Swiss-Prot for OTULIN Gene

Cytoplasm.

Gene Ontology (GO) - Cellular Components for OTULIN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA 23746843
GO:0071797 LUBAC complex IEA --
genes like me logo Genes that share ontologies with OTULIN: view

Pathways for OTULIN Gene

SuperPathways for OTULIN Gene

No Data Available

Gene Ontology (GO) - Biological Process for OTULIN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002040 sprouting angiogenesis ISS --
GO:0006508 proteolysis --
GO:0032088 negative regulation of NF-kappaB transcription factor activity IMP 23806334
GO:0045087 innate immune response IMP 23806334
GO:0050728 negative regulation of inflammatory response IMP 23806334
genes like me logo Genes that share ontologies with OTULIN: view

No data available for Pathways by source for OTULIN Gene

Transcripts for OTULIN Gene

Alternative Splicing Database (ASD) splice patterns (SP) for OTULIN Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b ^ 7
SP1: - -
SP2: - - -
SP3:
SP4:

Relevant External Links for OTULIN Gene

GeneLoc Exon Structure for
OTULIN
ECgene alternative splicing isoforms for
OTULIN

Expression for OTULIN Gene

mRNA expression in normal human tissues for OTULIN Gene

Integrated Proteomics: protein expression from ProteomicsDB and MOPED for OTULIN Gene

genes like me logo Genes that share expressions with OTULIN: view

Orthologs for OTULIN Gene

This gene was present in the common ancestor of chordates.

Orthologs for OTULIN Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia FAM105B 36
  • 99.9 (n)
  • 100 (a)
FAM105B 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia FAM105B 36
  • 86.55 (n)
  • 88.64 (a)
FAM105B 37
  • 89 (a)
OneToOne
dog
(Canis familiaris)
Mammalia FAM105B 36
  • 89.2 (n)
  • 90.34 (a)
FAM105B 37
  • 90 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Fam105b 36
  • 87.69 (n)
  • 90.06 (a)
Fam105b 16
Fam105b 37
  • 90 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia FAM105B 37
  • 78 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia FAM105B 37
  • 80 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Fam105b 36
  • 86.84 (n)
  • 87.78 (a)
chicken
(Gallus gallus)
Aves FAM105B 36
  • 66.45 (n)
  • 61.18 (a)
FAM105B 37
  • 55 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia FAM105B 37
  • 59 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia fam105b 36
  • 60.9 (n)
  • 57.61 (a)
zebrafish
(Danio rerio)
Actinopterygii fam105ba 37
  • 15 (a)
OneToMany
fam105bb 37
  • 33 (a)
OneToMany
LOC100001665 36
  • 56.39 (n)
  • 47.26 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 41 (a)
OneToMany
Species with no ortholog for OTULIN:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for OTULIN Gene

ENSEMBL:
Gene Tree for OTULIN (if available)
TreeFam:
Gene Tree for OTULIN (if available)

Paralogs for OTULIN Gene

Paralogs for OTULIN Gene

genes like me logo Genes that share paralogs with OTULIN: view

Variants for OTULIN Gene

Sequence variations from dbSNP and Humsavar for OTULIN Gene

SNP ID Clin Chr 05 pos Sequence Context AA Info Type MAF
rs25941 -- 14,680,342(+) TATGA(C/T)TTGAA intron-variant
rs25945 -- 14,680,138(+) GTACA(A/G)CTGCA intron-variant
rs25951 -- 14,693,371(+) TTTAT(A/G)TCTCA utr-variant-3-prime
rs25952 -- 14,695,138(+) TGCTT(A/C)TGTCC utr-variant-3-prime
rs27882 -- 14,680,030(+) GGCGT(A/G)ATTGT intron-variant

Relevant External Links for OTULIN Gene

HapMap Linkage Disequilibrium report
OTULIN

No data available for Structural Variations from Database of Genomic Variants (DGV) for OTULIN Gene

Disorders for OTULIN Gene

No disorders were found for OTULIN Gene.

No data available for UniProtKB/Swiss-Prot for OTULIN Gene

Publications for OTULIN Gene

  1. The linear ubiquitin-specific deubiquitinase gumby regulates angiogenesis. (PMID: 23708998) Rivkin E. … Cordes S.P. (Nature 2013) 2 3 4
  2. OTULIN antagonizes LUBAC signaling by specifically hydrolyzing Met1- linked polyubiquitin. (PMID: 23746843) Keusekotten K. … Komander D. (Cell 2013) 2 3 4
  3. OTULIN restricts Met1-linked ubiquitination to control innate immune signaling. (PMID: 23806334) Fiil B.K. … Gyrd-Hansen M. (Mol. Cell 2013) 3 4
  4. OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis. (PMID: 23827681) Mevissen T.E. … Komander D. (Cell 2013) 3 4
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4

Products for OTULIN Gene

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat
  • QIAGEN qRT-PCR Assays for microRNAs that regulate OTULIN
    • QuantiTect SYBR Green Assays in human,mouse,rat
    • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
    • QuantiFast Probe-based Assays in human,mouse,rat
    • Predesigned siRNA for gene silencing in human,mouse,rat for OTULIN
    • Block miRNA regulation of OTULIN using miScript Target Protectors

    Sources for OTULIN Gene

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