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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

OTUB2 Gene

protein-coding   GIFtS: 49
GCID: GC14P094492

OTU domain, ubiquitin aldehyde binding 2

(Previous name: chromosome 14 open reading frame 137 )
(Previous symbol: C14orf137)
  Search for OTUB2
in our new
 Human Malady Compendium 
Biological research products
for OTUB2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
OTU Domain, Ubiquitin Aldehyde Binding 21 2     MGC31021
C14orf1371 2 3 5     Chromosome 14 Open Reading Frame 1371
OTB22 3 5     Otubain-23
OTU22 3 5     Ubiquitin Thioesterase OTUB22
Deubiquitinating Enzyme OTUB22 3     Ubiquitin-Specific Protease Otubain 22
Ubiquitin-Specific-Processing Protease OTUB22 3     EC 3.4.19.123
OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 22 3     Otubain-23
FLJ219161     

External Ids:    HGNC: 203511   Entrez Gene: 789902   Ensembl: ENSG000000897237   OMIM: 6083385   UniProtKB: Q96DC93   

Export aliases for OTUB2 gene to outside databases

Previous GC identifers: GC14P092483 GC14P093562 GC14P074672


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for OTUB2:
This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their
stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU
domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. (provided
by RefSeq, Aug 2011)

UniProtKB/Swiss-Prot: OTUB2_HUMAN, Q96DC9
Function: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important
regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both
'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains

Gene Wiki entry for OTUB2


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000014.8  NC_018925.1  NT_026437.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the OTUB2 gene promoter:
         LHX3b/Lhx3b   p53   ATF-2   POU6F1 (c2)   POU2F1   POU2F1a   HNF-1   LHX3a/Lhx3a   c-Jun   ATF   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidOTUB2 promoter sequence
   Search SABiosciences Chromatin IP Primers for OTUB2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat OTUB2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q32.12   Ensembl cytogenetic band:  14q32.12   HGNC cytogenetic band: 14q32.13-q32.2

OTUB2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
OTUB2 gene location

GeneLoc information about chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14P094492:  view genomic region     (about GC identifiers)

Start:
94,492,675 bp from pter      End:
94,515,276 bp from pter
Size:
22,602 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: OTUB2_HUMAN, Q96DC9 (See protein sequence)
Recommended Name: Ubiquitin thioesterase OTUB2  
Size: 234 amino acids; 27213 Da
Miscellaneous: In the structure described by PubMed:15258613, the Asp-48 active site of the catalytic triad is located
too far to interact directly with the active site His-224. A possible explanation is that OTUB2 is in inactive
conformation in absence of ubiquitin and a conformation change may move Asp-48 in the proximity of His-224 in presence
of ubiquitin substrate
1 PDB 3D structure from and Proteopedia for OTUB2:
1TFF (3D)    
Secondary accessions: Q6IA10 Q9H6T1
Alternative splicing: 2 isoforms:  Q96DC9-1   Q96DC9-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for OTUB2: NX_Q96DC9

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96DC9

  • OTUB2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_075601.1  
    ENSEMBL proteins: 
     ENSP00000203664   ENSP00000451283  

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    ProSpec Recombinant Protein for OTUB2
    Uscn Proteins for OTUB2

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575cellular_component ND--


    OTUB2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    OTUB2 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR003323 OTU
     IPR016615 Ubiquitin_thioesterase_Otubain
     IPR019400 Peptidase_C65_otubain

    Graphical View of Domain Structure for InterPro Entry Q96DC9

    ProtoNet protein and cluster: Q96DC9

    UniProtKB/Swiss-Prot: OTUB2_HUMAN, Q96DC9
    Similarity: Belongs to the peptidase C65 family
    Similarity: Contains 1 OTU domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: OTUB2_HUMAN, Q96DC9
    Function: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important
    regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both
    'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains
    Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the
    C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)

    Enzyme Number (IUBMB): EC 3.4.19.121

    miRNA
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    hsa-let-7d hsa-miR-1224-3p hsa-miR-29a hsa-miR-218 hsa-miR-766 hsa-let-7g hsa-miR-149 hsa-let-7a
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004843ubiquitin-specific protease activity IMP15258613
    GO:0008242omega peptidase activity IEA--


    OTUB2 for ontologies           About GeneDecksing


    Animal Models:
         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Otub2):
     hematopoietic system  immune system 

    OTUB2 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for OTUB2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 11)

    5/13 Interacting proteins for OTUB2 (Q96DC93 ENSP000002036644) via UniProtKB, MINT, STRING, and/or I2D (see all 13)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ATXN3P542523, ENSP000003523244I2D: score=2 STRING: ENSP00000352324
    GTF2IP783473, ENSP000003225424I2D: score=2 STRING: ENSP00000322542
    MAVSQ7Z4343, ENSP000004019804I2D: score=1 STRING: ENSP00000401980
    UBE2NP610883, ENSP000003161764I2D: score=1 STRING: ENSP00000316176
    TRAF3Q131143, ENSP000003324684I2D: score=1 STRING: ENSP00000332468
    About this table

    Gene Ontology (GO): 5 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508proteolysis IEA--
    GO:0006520cellular amino acid metabolic process IDA12704427
    GO:0016579protein deubiquitination IMP15258613
    GO:0070536protein K63-linked deubiquitination IDA18954305
    GO:0071108protein K48-linked deubiquitination IDA18954305


    OTUB2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for OTUB2
    Search CenterWatch for drugs/clinical trials and news about OTUB2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for OTUB2 gene: 
    NM_023112.3  

    Unigene Cluster for OTUB2:

    OTU domain, ubiquitin aldehyde binding 2
    Hs.278815  [show with all ESTs]
    Unigene Representative Sequence: AK025569
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000203664(uc001yci.3) ENST00000553723(uc001ych.3)

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    hsa-let-7d hsa-miR-1224-3p hsa-miR-29a hsa-miR-218 hsa-miR-766 hsa-let-7g hsa-miR-149 hsa-let-7a
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    Additional cDNA sequence: 

    AF318378.1 AK025569.1 AY177201.1 BC000208.1 BC009615.1 BC068058.1 CR457345.1 

    5 DOTS entries:

    DT.310192  DT.91664155  DT.100746766  DT.92045917  DT.95262713 

    24/70 AceView cDNA sequences (see all 70):

    AI948840 AI401121 AF318378 BM667366 BM750394 BM710646 AA402461 BC009615 
    NM_023112 BF223906 CA309811 BC000208 BE255908 AI807115 BX100551 CF529334 
    F02820 BG718581 AK025569 CR457345 BF981619 AY177201 D44967 AA398564 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    OTUB2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: ATGGATGTAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See OTUB2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for OTUB2

    SOURCE GeneReport for Unigene cluster: Hs.278815

    UniProtKB/Swiss-Prot: OTUB2_HUMAN, Q96DC9
    Tissue specificity: Widely expressed. Expressed at higher level in brain

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for OTUB2 gene from 5/20 species (see all 20)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Otub21 , 5 OTU domain, ubiquitin aldehyde binding 21, 5 91.7(n)1
    94.85(a)1
      12 (52.92 cM)5
    681491  NM_001177841.11  NP_001171312.11 
     1033886825 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG49686
    --
    33(a)
    1 → many
    2L(10306922-10308045)
    worm
    (Caenorhabditis elegans)
    Secernentea otub-16
    Ubiquitin thioesterase otubain-like
    23(a)
    possible ortholog
    V(15066279-15067447)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT1G281206
    ubiquitin thioesterase otubain-like protein
    29(a)
    1 → many
    1(9812959-9815544)
    rice
    (Oryza sativa)
    Liliopsida --
    --
    expressed protein
    31(a)
    12(a)
    many → 1
    possible ortholog
    8(26880241-26884649)
    4(33060082-33066244)


    ENSEMBL Gene Tree for OTUB2 (if available)
    TreeFam Gene Tree for OTUB2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for OTUB2 gene
    OTUB12  
    2 SIMAP similar genes for OTUB2 using alignment to 3 protein entries:     OTUB2_HUMAN (see all proteins):
    OTUB1    DKFZp564E242

    OTUB2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/505 NCBI SNPs in OTUB2 are shown (see all 505    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 14 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1844944601,2
    --94490732(+) ATGGGC/TGGCCC 1 -- us2k10--------
    rs2019944501,2
    --94490732(+) ATGGG-/CGGCCC 1 -- us2k10--------
    rs1144346871,2
    C,--94490733(+) TGGGCA/GGCCCC 1 -- us2k10--------
    rs1399580351,2
    --94490845(+) GAATCC/TTGATT 1 -- us2k10--------
    rs101489741,2
    C,F,--94490868(+) GAAATT/CACATT 1 -- us2k15Minor allele frequency- C:0.15NA WA CSA 126
    rs1815706091,2
    --94490951(+) GTGTGG/TGTGTG 1 -- us2k10--------
    rs611413551,2
    C,F,--94491099(+) GCAGTG/AAGTGG 1 -- us2k14Minor allele frequency- A:0.13WA CSA 123
    rs1438944331,2
    --94491175(+) GGCTTA/GGTTTG 1 -- us2k10--------
    rs1472782801,2
    --94491198(+) CCTGAA/GGAAGG 1 -- us2k10--------
    rs776495261,2
    F,--94491218(+) GAGCCC/TAGGAC 1 -- us2k11Minor allele frequency- T:0.09WA 118

    HapMap Linkage Disequilibrium report for OTUB2 (94492675 - 94515276 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for OTUB2
         1 CNV: 47863

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    OTUB2 for disorders           About MalaCards

    OTUB2 for disorders           About GeneDecksing

    OMIM gene information: 608338    OMIM disorders: --


    Export disorders for OTUB2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for OTUB2 gene, integrated from 9 sources (see all 20):
    (articles sorted by number of sources associating them with OTUB2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Otubains: a new family of cysteine proteases in the ubiquitin pathway. (PubMed id 12704427)1, 2, 3, 9 Balakirev M.Y.... Chroboczek J. (2003)
    2. Crystal structure of human otubain 2. (PubMed id 15258613)1, 2, 9 Nanao M.H....Balakirev M.Y. (2004)
    3. Structural basis and specificity of human otubain 1-mediated deubiquitination. (PubMed id 18954305)1, 2 Edelmann M.J.... Kessler B.M. (2009)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    6. The DNA sequence and analysis of human chromosome 14. (PubMed id 12508121)1, 2 Heilig R.... Weissenbach J. (2003)
    7. A microarray of ubiquitylated proteins for profiling d eubiquitylase activity reveals the critical roles of both chain and substrate. (PubMed id 22626734)1 Loch C.M. and Strickler J.E. (2012)
    8. Activity-based chemical proteomics accelerates inhibi tor development for deubiquitylating enzymes. (PubMed id 22118674)1 Altun M....Kessler B.M. (2011)
    9. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    10. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 78990 HGNC: 20351 AceView: OTUB2 Ensembl:ENSG00000089723 euGenes: HUgn78990
    ECgene: OTUB2 H-InvDB: OTUB2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for OTUB2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for OTUB2 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for OTUB2 gene:
    Search GeneIP for patents involving OTUB2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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