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OTUB1 Gene

protein-coding   GIFtS: 60
GCID: GC11P063753

OTU Deubiquitinase, Ubiquitin Aldehyde Binding 1

(Previous names: OTU domain, ubiquitin aldehyde binding 1)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
OTU Deubiquitinase, Ubiquitin Aldehyde Binding 11 2     otubain-12
OTB12 3 5     Ubiquitin Thioesterase OTUB12
OTU12 3 5     Ubiquitin-Specific Protease Otubain 12
OTU Domain, Ubiquitin Aldehyde Binding 11 2     EC 3.4.19.123
Deubiquitinating Enzyme OTUB12 3     Otubain-13
Ubiquitin-Specific-Processing Protease OTUB12 3     hOTU13
OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 12 3     EC 3.1.28
OTU-Domain Ubal-Binding 12     

External Ids:    HGNC: 230771   Entrez Gene: 556112   Ensembl: ENSG000001677707   OMIM: 6083375   UniProtKB: Q96FW13   

Export aliases for OTUB1 gene to outside databases

Previous GC identifers: GC11P063529 GC11P063510 GC11P060082


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for OTUB1 Gene:
The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The
encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin
chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin
ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific
ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth.
Alternative splicing results in multiple transcript variants. (provided by RefSeq, Jul 2008)

GeneCards Summary for OTUB1 Gene:
OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1) is a protein-coding gene. GO annotations related to this gene include ubiquitin binding and ubiquitin protein ligase binding. An important paralog of this gene is OTUB2.

UniProtKB/Swiss-Prot: OTUB1_HUMAN, Q96FW1
Function: Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an
important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy,
a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to
their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy.
Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes
anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated
RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked
polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable
of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin
Function: Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3
ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites.
Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2
partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by
binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13
and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that
mimics a cleaved 'Lys48'-linked di-ubiquitin chain

Gene Wiki entry for OTUB1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000011.9  NC_018922.2  NT_167190.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the OTUB1 gene promoter:
         Elk-1   RFX1   NF-1   NF-1/L   HSF1 (long)   NRF-2   Sox9   HSF1short   STAT3   Zic3   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidOTUB1 promoter sequence
   Search Chromatin IP Primers for OTUB1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat OTUB1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q13.1   Ensembl cytogenetic band:  11q13.1   HGNC cytogenetic band: 11q13.1

OTUB1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
OTUB1 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P063753:  view genomic region     (about GC identifiers)

Start:
63,753,325 bp from pter      End:
63,769,283 bp from pter
Size:
15,959 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: OTUB1_HUMAN, Q96FW1 (See protein sequence)
Recommended Name: Ubiquitin thioesterase OTUB1  
Size: 271 amino acids; 31284 Da
Subunit: Isoform 1 and isoform 2 interact with RNF128. Isoform 1 forms a ternary complex with RNF128 and USP8.
Isoform 1 interacts with the C-terminal UCH catalytic domain of USP8. Isoform 2 does not associate with USP8.
Interacts with FUS, ESR1 and GNB2L1/RACK1. Interacts with UBE2N/UBC13
Miscellaneous: In the structure described by PubMed:18954305, the His-265 active site of the catalytic triad is
located too far to interact directly with the active site Cys-91. A possible explanation is that OTUB1 is in
inactive conformation in absence of ubiquitin and a conformation change may move His-265 in the proximity of
Cys-91 in presence of ubiquitin substrate
Sequence caution: Sequence=AAF28941.1; Type=Erroneous initiation;
Selected PDB 3D structures from and Proteopedia for OTUB1 (see all 7):
2ZFY (3D)        3VON (3D)        4DDG (3D)        4DDI (3D)        4DHZ (3D)        4I6L (3D)    
Secondary accessions: Q32Q78 Q96II3 Q9NXQ4 Q9P0B8
Alternative splicing: 2 isoforms:  Q96FW1-1   Q96FW1-2   (Lacks the catalytic sites for protease activity)

Explore the universe of human proteins at neXtProt for OTUB1: NX_Q96FW1

Explore proteomics data for OTUB1 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys71, Lys84, Lys109, Lys188, Lys201
  • Modification sites at PhosphoSitePlus
  • 4 DME Specific Peptides for OTUB1 (Q96FW1)
     FPEGSEP  DRGEGGT  TRPDGNCF  KESDHIHIIAL 


    See OTUB1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_060140.2  
    ENSEMBL proteins: 
     ENSP00000439142   ENSP00000440211   ENSP00000402551   ENSP00000444357   ENSP00000437453  
     ENSP00000441328   ENSP00000444968   ENSP00000416973  

    OTUB1 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant OTUB1 Protein
    R&D Systems Recombinant & Natural Proteins for OTUB1 (Otubain-1)
    Enzo Life Sciences proteins for OTUB1
    OriGene Purified Protein for OTUB1
    OriGene Protein Over-expression Lysate for OTUB1
    OriGene MassSpec for OTUB1
    OriGene Custom Protein Services for OTUB1
    GenScript Custom Purified and Recombinant Proteins Services for OTUB1
    Novus Biologicals OTUB1 Proteins
    Novus Biologicals OTUB1 Lysate
    Sino Biological Recombinant Protein for OTUB1
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for OTUB1
    Cloud-Clone Corp. Proteins for OTUB1

     
    Search eBioscience for Proteins for OTUB1 

     
    antibodies-online proteins for OTUB1 (19 products) 

     
    antibodies-online peptides for OTUB1

    OTUB1 Antibody Products:

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    Novus Biologicals OTUB1 Antibodies
    Abcam antibodies for OTUB1
    Cloud-Clone Corp. Antibodies for OTUB1
    ThermoFisher Antibody for OTUB1
    antibodies-online antibodies for OTUB1 (65 products) 

    OTUB1 Assay Products:

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    Cloud-Clone Corp. ELISAs for OTUB1
    Cloud-Clone Corp. CLIAs for OTUB1
    Search eBioscience for ELISAs for OTUB1 
    antibodies-online kits for OTUB1 (6 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    OTUD: OTU domain containing

    3 InterPro protein domains:
     IPR003323 OTU
     IPR016615 Ubiquitin_thioesterase_Otubain
     IPR019400 Peptidase_C65_otubain

    Graphical View of Domain Structure for InterPro Entry Q96FW1

    ProtoNet protein and cluster: Q96FW1

    UniProtKB/Swiss-Prot: OTUB1_HUMAN, Q96FW1
    Domain: In addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also
    present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct
    binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-,
    'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination
    Similarity: Belongs to the peptidase C65 family
    Similarity: Contains 1 OTU domain


    Find genes that share domains with OTUB1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: OTUB1_HUMAN, Q96FW1
    Function: Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an
    important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy,
    a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to
    their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy.
    Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes
    anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated
    RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked
    polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable
    of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin
    Function: Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3
    ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites.
    Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2
    partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by
    binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13
    and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that
    mimics a cleaved 'Lys48'-linked di-ubiquitin chain
    Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by
    the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)
    Enzyme regulation: By free ubiquitin: binding of free ubiquitin triggers conformational changes in the OTU domain
    and formation of a ubiquitin-binding helix in the N-terminus, promoting binding of the conjugated donor ubiquitin
    in UBE2N/UBC13 to OTUB1

         Enzyme Numbers (IUBMB): EC 3.1.22 EC 3.4.19.121

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004843ubiquitin-specific protease activity IDA18954305
    GO:0005515protein binding IPI16364312
    GO:0008242omega peptidase activity IEA--
    GO:0019784NEDD8-specific protease activity IDA18954305
    GO:0031625ubiquitin protein ligase binding IPI--
         
    Find genes that share ontologies with OTUB1           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for OTUB1:
     Decreased Wnt reporter activit  Decreased cilium length after  

    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for OTUB1

    miRNA
    Products:
        
    miRTarBase miRNAs that target OTUB1:
    hsa-let-7e-5p (MIRT051514), hsa-mir-93-3p (MIRT038748), hsa-let-7b-5p (MIRT051986), hsa-mir-28-5p (MIRT006414), hsa-mir-708-3p (MIRT006415)

    Block miRNA regulation of human, mouse, rat OTUB1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate OTUB1 (see all 17):
    hsa-miR-1226 hsa-miR-197 hsa-miR-661 hsa-miR-342-5p hsa-miR-23a hsa-miR-28-5p hsa-miR-548d-3p hsa-miR-634
    SwitchGear 3'UTR luciferase reporter plasmidOTUB1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for OTUB1
    Predesigned siRNA for gene silencing in human, mouse, rat OTUB1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for OTUB1

    Clone
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    OriGene clones in human, mouse for OTUB1 (see all 7)
    OriGene ORF clones in mouse, rat for OTUB1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: OTUB1 (NM_017670)
    Sino Biological Human cDNA Clone for OTUB1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for OTUB1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat OTUB1
    Addgene plasmids for OTUB1 

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for OTUB1
    Browse ESI BIO Cell Lines and PureStem Progenitors for OTUB1 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for OTUB1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    OTUB1_HUMAN, Q96FW1: Cytoplasm (By similarity)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol3
    nucleus2
    cytoskeleton1
    mitochondrion1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--
    GO:0070062extracellular vesicular exosome IDA19056867

    Find genes that share ontologies with OTUB1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for OTUB1 About    
    See pathways by source

    SuperPathContained pathways About
    1Cell Cycle / Checkpoint Control
    Cell Cycle / Checkpoint Control0.32
    DNA Damage0.32
    2Protein Stability
    Protein Stability


    Find genes that share SuperPaths with OTUB1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    3 Cell Signaling Technology (CST) Pathways for OTUB1
        Cell Cycle / Checkpoint Control
    DNA Damage
    Protein Stability



        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for OTUB1
    Interactions:

        Search GeneGlobe Interaction Network for OTUB1

    Selected Interacting proteins for OTUB1 (Q96FW11, 2, 3) via UniProtKB, MINT, STRING, and/or I2D (see all 57)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TP53P046371, 2, 3EBI-1058491,EBI-366083 MINT-8380008 MINT-8380032 MINT-8388795 MINT-8380020 MINT-8380053 MINT-8380061 MINT-8380636 MINT-8388765 I2D: score=1 
    ypkAQ569211, 2EBI-1058491,EBI-2849107 MINT-73525 MINT-73524 MINT-73527 EBI-1058491,EBI-8022937 MINT-7717193 MINT-7717390 MINT-7717207 MINT-7717075 MINT-7717170
    UBE2D2P628371, 2, 3EBI-1058491,EBI-347677 MINT-8388718 MINT-8388703 MINT-8388563 I2D: score=5 
    UBE2D1P516681, 2, 3EBI-1058491,EBI-743540 MINT-8388546 MINT-8388751 MINT-8388734 I2D: score=2 
    UBE2D3P610771, 2, 3EBI-1058491,EBI-348268 MINT-8388587 MINT-8388689 MINT-8388575 I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0002376immune system process IEA--
    GO:0006281DNA repair IEA--
    GO:0006508proteolysis ----
    GO:0006974cellular response to DNA damage stimulus IDA--
    GO:0019538protein metabolic process ----

    Find genes that share ontologies with OTUB1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for OTUB1



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

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    REFSEQ mRNAs for OTUB1 gene: 
    NM_017670.2  

    Unigene Cluster for OTUB1:

    OTU domain, ubiquitin aldehyde binding 1
    Hs.473788  [show with all ESTs]
    Unigene Representative Sequence: NR_003089
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000301453(uc001nyf.1 uc001nyg.1 uc010rna.1) ENST00000541478
    ENST00000535715 ENST00000428192 ENST00000538426(uc009ypb.1) ENST00000543004
    ENST00000447683 ENST00000536443 ENST00000543988 ENST00000535140 ENST00000536943
    ENST00000422031
    miRNA
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    Block miRNA regulation of human, mouse, rat OTUB1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate OTUB1 (see all 17):
    hsa-miR-1226 hsa-miR-197 hsa-miR-661 hsa-miR-342-5p hsa-miR-23a hsa-miR-28-5p hsa-miR-548d-3p hsa-miR-634
    SwitchGear 3'UTR luciferase reporter plasmidOTUB1 3' UTR sequence
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    GenScript: all cDNA clones in your preferred vector: OTUB1 (NM_017670)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for OTUB1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat OTUB1
    Addgene plasmids for OTUB1 
    Primer
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    OriGene qPCR primer pairs and template standards for OTUB1
    OriGene qSTAR qPCR primer pairs in human, mouse for OTUB1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat OTUB1
      QuantiTect SYBR Green Assays in human, mouse, rat OTUB1
      QuantiFast Probe-based Assays in human, mouse, rat OTUB1

    Additional mRNA sequence: 

    AF161381.1 AK000120.1 AK098029.1 AK225133.1 AK298287.1 AK303228.1 AK307413.1 AL096714.1 
    AY177200.1 BC007519.2 BC010368.1 BC107701.1 NR_003089.1 

    Selected DOTS entries (see all 26):

    DT.100673331  DT.40107023  DT.100044589  DT.65286277  DT.95194975  DT.100044586  DT.120741968  DT.97856606 
    DT.120741804  DT.120741806  DT.97862416  DT.95375388  DT.120741893  DT.92466777  DT.100673325  DT.100673328 
    DT.101984460  DT.120741816  DT.120741881  DT.120741939  DT.120741964  DT.120741831  DT.120741845  DT.120741943 

    Selected AceView cDNA sequences (see all 1333):

    CR620541 CB112348 AA323553 F26706 BQ687690 CR604649 BM783268 F29137 
    BX394130 CR407687 CB109704 BQ420337 AI292187 BE219634 BG341135 BU783920 
    CD628458 F29044 BX341974 BM745217 AI298758 AI499007 BG820247 BU500805 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for OTUB1 (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10a · 10b
    SP1:                          -           -           -           -                 -               
    SP2:                          -           -           -           -                                 
    SP3:                                      -           -           -                                 
    SP4:                          -           -           -                                             
    SP5:                          -                       -                                             


    ECgene alternative splicing isoforms for OTUB1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    OTUB1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTTTATTAAA
    OTUB1 Expression
    About this image


    OTUB1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 11) fully expand
     
     Brain (Nervous System)    fully expand to see all 3 entries
             Cerebral Cortex
     
     Limb (Muscoskeletal System)    fully expand to see all 2 entries
             Limb Bud Mesenchyme Cells Limb Bud
             Limb Bud
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Mesoderm (Gastrulation Derivatives)
             Limb Bud Mesenchyme Cells Limb Bud
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
    OTUB1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    OTUB1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.473788

    UniProtKB/Swiss-Prot: OTUB1_HUMAN, Q96FW1
    Tissue specificity: Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils,
    lymph nodes and spleen, as well as peripheral blood mononuclear cells

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for OTUB1 gene from Selected species (see all 19)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Otub15 OTU domain, ubiquitin aldehyde binding 1   --   19 (5.27 cM) 7198202 
    chicken
    (Gallus gallus)
    Aves OTUB11 OTU domain, ubiquitin aldehyde binding 1 80(n)
    81.85(a)
      777320  XM_001236781.3  XP_001236782.2 
    lizard
    (Anolis carolinensis)
    Reptilia OTUB16
    OTU domain, ubiquitin aldehyde binding 1
    85(a)
    1 ↔ 1
    GL343555.1(16256-30093)
    African clawed frog
    (Xenopus laevis)
    Amphibia BJ036970.12   -- 73.55(n)    BJ036970.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufa19h072 Transcribed sequence with moderate similarity to protein more 77.77(n)    CK400405.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG49686
    --
    53(a)
    1 → many
    2L(10306922-10308045)
    worm
    (Caenorhabditis elegans)
    Secernentea otub-16
    Protein OTUB-1 (otub-1) mRNA, complete cds
    30(a)
    1 → many
    V(15066298-15067464) WBGene00007718
    barley
    (Hordeum vulgare)
    Liliopsida Hv.120302 Transcribed sequence with moderate similarity to protein more 78.44(n)    BJ468425.1 


    ENSEMBL Gene Tree for OTUB1 (if available)
    TreeFam Gene Tree for OTUB1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for OTUB1 gene
    OTUB22  
    2 SIMAP similar genes for OTUB1 using alignment to 7 protein entries:     OTUB1_HUMAN (see all proteins):
    DKFZp564E242    OTUB2

    Find genes that share paralogs with OTUB1           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for OTUB1 (see all 308)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs113511041,2
    C--63672840(+) AAAAA-/A/AA  
            
    TGTTA
    2 -- us2k1 trp31NA 2
    rs1508379641,2
    --63751496(+) CTGTCA/GCACAG 2 -- us2k10--------
    rs1912818661,2
    --63751784(+) AATAAA/GCCATC 2 -- us2k10--------
    rs618846721,2
    C,F--63751818(+) AGGTGC/TGGTGG 2 -- us2k14Minor allele frequency- T:0.48NA EA 244
    rs1398793521,2
    C--63752028(+) GGTTGA/CGGTGA 2 -- us2k10--------
    rs1143538451,2
    C--63752154(+) CCCAGG/TCTGGT 2 -- us2k10--------
    rs1156071911,2
    C,F--63752165(+) CTCCAA/GTGAAA 2 -- us2k13Minor allele frequency- G:0.07WA NA EA 358
    rs1473312921,2
    C--63752277(+) GCTCAC/TGCCTG 2 -- us2k10--------
    rs112316681,2
    C,F,H--63752509(+) ACTCCA/GGCCTG 2 -- us2k15Minor allele frequency- G:0.01NS EA 480
    rs340839111,2
    C--63752540(+) GTCTC-/AAAAAA 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for OTUB1 (63753325 - 63769283 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 8 variations for OTUB1:    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv1155n71CNV Loss21882294
    esv29993CNV Loss17803354
    nsv897642CNV Loss21882294
    dgv1159n71CNV Loss21882294
    dgv1156n71CNV Loss21882294
    nsv897648CNV Loss21882294
    nsv897639CNV Loss21882294
    dgv1160n71CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 608337    OMIM disorders: --


    Find genes that share disorders with OTUB1           About GenesLikeMe


    Export disorders for OTUB1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for OTUB1 gene, integrated from 10 sources (see all 59):
    (articles sorted by number of sources associating them with OTUB1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. OTU Domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) deubiquitinates estrogen receptor (ER) alpha and affects ERalpha transcriptional activity. (PubMed id 19383985)1, 2, 3, 9 Stanisic V.... O'Malley B.W. (J. Biol. Chem. 2009)
    2. Otubains: a new family of cysteine proteases in the ubiquitin pathway. (PubMed id 12704427)1, 2, 3 Balakirev M.Y.... Chroboczek J. (EMBO Rep. 2003)
    3. Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1. (PubMed id 19211026)1, 2, 9 Wang T.... Wolberger C. (J. Mol. Biol. 2009)
    4. Structural basis and specificity of human otubain 1-mediated deubiquitination. (PubMed id 18954305)1, 2, 9 Edelmann M.J.... Kessler B.M. (Biochem. J. 2009)
    5. OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function. (PubMed id 22325355)1, 2 Juang Y.C....Durocher D. (Mol. Cell 2012)
    6. The mechanism of OTUB1-mediated inhibition of ubiquitination. (PubMed id 22367539)1, 2 Wiener R.... Wolberger C. (Nature 2012)
    7. Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. (PubMed id 20725033)1, 2 Nakada S.... Durocher D. (Nature 2010)
    8. Two isoforms of otubain 1 regulate T cell anergy via GRAIL. (PubMed id 14661020)1, 2 Soares L.... Fathman C.G. (Nat. Immunol. 2004)
    9. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 55611 HGNC: 23077 AceView: COX8AandOTUB1 Ensembl:ENSG00000167770 euGenes: HUgn55611
    ECgene: OTUB1 H-InvDB: OTUB1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for OTUB1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for OTUB1 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for OTUB1 gene:
    Search GeneIP for patents involving OTUB1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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