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OS9 Gene

protein-coding   GIFtS: 54

GC12P056375
osteosarcoma amplified 9, endoplasmic reticulum associated protein
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
OS-9 1, 2
Descriptions
Amplified in osteosarcoma 9 3
amplified in osteosarcoma 2
osteosarcoma amplified 9 2
osteosarcoma amplified 9, endoplasmic reticulum associated
protein 2
External Ids
HGNC: 169941
Entrez Gene: 109562
UniProtKB: Q134383
Ensembl: ENSG000001355067
Search outside databases for aliases for OS9 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for OS9:
This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the
hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and
promotes the degradation of one of its subunits. Alternate transcriptional splice variants,
encoding different isoforms, have been characterized. [provided by RefSeq]

UniProtKB/Swiss-Prot: OS9_HUMAN, Q13438
Function: May play a role in the ER-associated degradation of misfolded glycoproteins

Gene Wiki entry for OS9

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the OS9 gene  

Entrez Gene cytogenetic band: 12q13   Ensembl cytogenetic band:  12q14.1   HGNC cytogenetic band: 12q13

OS9 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P056375:     (about GC identifiers)

Start:
56,374,153 bp from pter
End:
56,401,607 bp from pter
Size:
27,455 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_029419.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: OS9_HUMAN, Q13438 (See protein sequence)
Recommended Name: Protein OS-9 precursor  
Size: 667 amino acids; 75562 Da
Secondary accessions: A6NDD1 O00579
Alternative splicing: 3 isoforms:  Q13438-1   Q13438-2   Q13438-3   

REFSEQ proteins (4 alternative transcripts): 
NP_001017956.1  NP_001017957.1  NP_001017958.1  NP_006803.1  


ENSEMBL proteins: 
ENSP00000373798 ENSP00000257966 ENSP00000318165 ENSP00000373794 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (OS9)
Human Recombinant Proteins from Abnova (OS9)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005625 soluble fraction TAS8634085
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Antibodies for OS9: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for OS9
Browse R&D Systems for Antibodies
Antibodies from Abcam (OS9), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (OS9)
Novus Biologicals Antibodies for OS9

Assays for OS9: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR012913 PRKCSH


   GeneDecks  OS9 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q13438

ProtoNet protein and cluster: Q13438

1 Blocks protein family: IPB012913 Glucosidase II beta subunit-like

UniProtKB/Swiss-Prot: OS9_HUMAN, Q13438
Similarity: Belongs to the OS-9 family
Similarity: Contains 1 PRKCSH domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OS9)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_001017956

              Applied Biosystems Silencer® siRNAs for OS9

              Sigma-Aldrich siRNA for OS9  
                     Sigma-Aldrich shRNA for OS9  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for OS9
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001017956
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001017956
                                 untagged cDNA clones in CMV expression vector (see all 4): NM_001017956 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_006812

UniProtKB/Swiss-Prot: OS9_HUMAN, Q13438
Function: May play a role in the ER-associated degradation of misfolded glycoproteins

Genatlas biochemistry entry for OS9:
homogeneously staining-region encoded sequence OS9,from an osteosarcoma-cell line,with three
isoforms,coamplified with CDK4,PRIM1,SAS in osteosarcoma

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IPI15721254
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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5/16 Interacting proteins for OS9 (ENSP000003181653 Q134381, 2) via UniProtKB, MINT, and/or STRING (see all 16 )
InteractantInteraction Details
GeneCardExternal ID(s)
EGLN3Q9H6Z91STRING (score=.977) EBI-1174342, EBI-1175354
HIF1AQ166651STRING (score=.993) EBI-1174351, EBI-447269
EGLN1Q9GZT91EBI-1174342, EBI-1174818
SMAD2Q157962MINT-60916
ZNF512BQ96KM62MINT-60815
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for OS9

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OS9)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_001017956

              Sigma-Aldrich siRNA for OS9  
                     Sigma-Aldrich shRNA for OS9  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001017956  NM_001017957  NM_001017958  NM_006812  

REFSEQ mRNAs for OS9 gene (4 alternative transcripts): 

NM_001017956.1   NM_001017957.1   NM_001017958.1   NM_006812.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001017956  NM_001017957  NM_001017958  NM_006812  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001017956
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001017956
                                 untagged cDNA clones in CMV expression vector (see all 4): NM_001017956 

Additional cDNA sequence: 

AB002806.1 AK129781.1 AK130733.1 AK291374.1 AK293435.1 AK294870.1 AK296770.1 AK298532.1 
AK303858.1 AK304533.1 BC000532.2 BC006506.2 BC007254.1 BC023513.2 CR456791.1 CR593783.1 
CR594965.1 CR600830.1 CR604702.1 CR610698.1 CR613922.1 CR616277.1 CR621694.1 EF036505.1 
U09408.1 U41635.1 

24/46 DOTS entries (see all 46 ):

DT.455567  DT.100682332  DT.100682338  DT.100894740  DT.100846873  DT.95165428  DT.86846445  DT.100875178 
DT.121176688  DT.121176686  DT.100682333  DT.100039316  DT.101965445  DT.121176647  DT.100682341  DT.95165476 
DT.100682353  DT.121176622  DT.100682335  DT.121176593  DT.100682357  DT.121176695  DT.40313741  DT.95165517 

24/962 AceView cDNA sequences (see all 962 ):

AI307136 BE047727 CB217031 CB145494 CF132317 CR594965 AA311351 CA445865 
AA364121 BM668805 AU132426 BQ707802 BQ181517 AA306439 AU133841 BE796257 
AU130105 BM707707 BE300142 BQ937790 AA923719 AW068865 BE295383 AI683030 

highest scoring ESTs for OS9:

U41635 AA013242 AA021448 AA025446 AA085848 AA130872 AA171903 AA196163 AA229456 AA229590 

Unigene Cluster for OS9:

Osteosarcoma amplified 9, endoplasmic reticulum associated protein
Hs.527861  [show with all ESTs]
Unigene Representative Sequence: U41635


GeneLoc Exon Structure

5/26 Alternative Splicing Database (ASD) splice patterns (SP) for OS9 (see all 26 )

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c ^ 8a · 8b · 8c · 8d ^ 9a · 9b · 9c ^ 10a · 10b ·
SP1:                          -                                         -                             -     -                       -                           
SP2:                                                                    -                             -     -                       -                           
SP3:                          -                                         -                             -     -                       -                           
SP4:                                                                                                                                -                           
SP5:                          -           -     -     -     -     -     -     -     -     -     -     -     -     -     -     -     -     -     -     -         

ExUns: 10c ^ 11a · 11b · 11c ^ 12 ^ 13 ^ 14a · 14b · 14c ^ 15a · 15b ^ 16a · 16b · 16c ^ 17a · 17b · 17c · 17d ^ 18a · 18b ^ 19 ^ 20a · 20b · 20c
SP1:        -                                         -                                                                                             
SP2:        -                                                                                                                                       
SP3:        -                                                                                                                                       
SP4:        -                                         -                                                                                             
SP5:        -                                                                                                                                       

About this scheme

ECgene alternative splicing isoforms for OS9

4 Ensembl transcripts including schematic representations:
ENST00000389146  ENST00000257966  ENST00000315970  ENST00000389142  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

OS9 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for OS9

1 / 2 / 3

6 probe-sets matching OS9 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

36996_at2, 3 U95-A 1 1.00 1.00 0.38 1.25 U41635 1.00 1.00 1.00 1
87781_s_at2, 3 U95-E 1 1.00 1.00 0.88 0.76 AI089439 0.60 1.00 0.82 1

200714_x_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006812 0.60 1.00 0.82 1

215399_s_at2, 3 U133-A 1 1.00 1.00 -- -- AI683900 0.40 1.00 0.76 1

200714_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

215399_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  OS9 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTCTTGAACA

SOURCE GeneReport for Unigene cluster: Hs.527861

UniProtKB/Swiss-Prot: OS9_HUMAN, Q13438
Tissue specificity: Ubiquitously expressed. Found as well in all tumor cell lines analyzed,
amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell
lines. OS-9-2 is the major isoform detected in all cell types examined

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for OS9 gene from 5/10 species (see all 10 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
LOC4744191   -- similar to amplified in osteosarcoma isoform 2 precursor 88.21(n)
88.91(a)
474419  XM_531650.2  XP_531650.2 
chimpanzee
(Pan troglodytes)
OS-91   -- amplified in osteosarcoma 99.75(n)
99.7(a)
452022  XM_509170.2  XP_509170.2 
cow
(Bos taurus)
OS91   -- amplified in osteosarcoma 90.69(n)
90.69(a)
504832  NM_001034225.1  NP_001029397.1 
rat
(Rattus norvegicus)
Os91   -- amplified in osteosarcoma 84.21(n)
83.16(a)
362891  NM_001007265.1  NP_001007266.1 
mouse
(Mus musculus)
Os91, 5 105
amplified in osteosarcoma1, 5 82.16(n)1
79.97(a)1
2164401  NM_177614.21  NP_808282.11 
 AI4661985  AI8439495  (see all 14)
About this table        Species with no ortholog for OS9

ENSEMBL Gene Tree for OS9
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for OS9 gene
C2orf302  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/201 NCBI SNPs in OS9 are shown (see all 201 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 70)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 12 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs47601681,2
A,C,F,H56374004(+) TGGTCT/GCTGAC 4 -- ng516Minor allele frequency- G:0.45MN EA EU WA 736
rs10500451,2
A,C,F,H56401538(+) CCCAGT/CGAAAA 4 -- ut318Minor allele frequency- C:0.27EU EA WA 838
rs47603191,2
A,C,F,H56373753(+) GCGTCT/CGTATA 4 -- ng5111Minor allele frequency- C:0.09EU EA WA NA 978
rs73116321,2
C,F,H56372607(+) CTATAA/CTATCT 4 -- ng51 tfbs38Minor allele frequency- C:0.26EU EA WA 824
rs347648111,2
C,F56398422(+) CCGTTC/TGGAGG 4 S/L mis15Minor allele frequency- T:0.03NA EU EA WA 492
--
rs16895891,2
F,H56372899(-) ctgtgG/Agagtc 4 -- ng514Minor allele frequency- A:0.06EU EA WA 420
--
rs16785051,2
A,C56372743(+) tcaatC/Gaattc 4 -- ng510--------
rs111723081,2
C,H56396478(+) ACCAAG/CGATGA 4 N/K mis14Minor allele frequency- C:0.00EU EA WA 416
rs10500511,2
A56401559(+) CACCCA/CCCATG 4 -- ut31 ese31Minor allele frequency- C:0.00MN 184
rs26194421,2
H56372157(+) ttttgT/Actgta 4 -- ng514Minor allele frequency- A:0.00EU EA WA 420
About this table

HapMap Linkage Disequilibrium images for OS9 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 609677

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/18 PubMed articles for OS9 gene (see all 18 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 10956 HGNC: 16994 AceView: OS-9 Ensembl:ENSG00000135506 euGenes: HUgn10956
ECgene: OS9 H-InvDB: OS9
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for OS9:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for OS9-related products

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 Millipore Preclinical / Clinical Development Services
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 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for OS9 Antibodies for OS9
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for OS9 Browse small molecules at Sigma-Aldrich
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 Antibodies for OS9

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