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OLFML3 Gene

protein-coding   GIFtS: 49

GC01P114323
olfactomedin-like 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
HNOEL-iso 1, 2, 3
OLF44 1, 2
OTTHUMP00000013761 2
hOLF44 3
Descriptions
olfactomedin-like 3 2
External Ids
HGNC: 249561
Entrez Gene: 569442
UniProtKB: Q9NRN53
Ensembl: ENSG000001167747
Search outside databases for aliases for OLFML3 gene

Previous GC identifers: GC01P113821 GC01P114234

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: OLFL3_HUMAN, Q9NRN5
Function: Secreted scaffold protein that plays an essential role in dorsoventral patterning during
early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal
side. Acts by facilitating the association between the tolloid proteases and their substrate
chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have
matrix-related function involved in placental and embryonic development, or play a similar role in
other physiological processes

Gene Wiki entry for OLFML3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the OLFML3 gene  

Entrez Gene cytogenetic band: 1p13.2   Ensembl cytogenetic band:  1p13.2   HGNC cytogenetic band: 1p13.1

OLFML3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P114323:     (about GC identifiers)

Start:
114,323,553 bp from pter
End:
114,326,399 bp from pter
Size:
2,847 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_019273.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: OLFL3_HUMAN, Q9NRN5 (See protein sequence)
Recommended Name: Olfactomedin-like protein 3 precursor  
Size: 406 amino acids; 46010 Da
Subcellular location: Secreted
Secondary accessions: Q53FR1 Q53HV9 Q5SQL6 Q69AX9 Q8NBJ2
Alternative splicing: 3 isoforms:  Q9NRN5-1   Q9NRN5-2   Q9NRN5-3   

REFSEQ proteins: NP_064575.1  

ENSEMBL proteins: 
ENSP00000322273 ENSP00000376977 ENSP00000358564 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
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Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (OLFML3)
              OriGene Purified Recombinant Human Protein: OLFML3 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005576 extracellular region IEA--
About this table

Antibodies for OLFML3: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for OLFML3

Assays for OLFML3: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR003112 Olfac-like


   GeneDecks  OLFML3 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9NRN5

ProtoNet protein and cluster: Q9NRN5

1 Blocks protein family: IPB003112 Olfactomedin-like

UniProtKB/Swiss-Prot: OLFL3_HUMAN, Q9NRN5
Similarity: Belongs to the OLFML3 family
Similarity: Contains 1 olfactomedin-like domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OLFML3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020190

              Applied Biosystems Silencer® siRNAs for OLFML3

              Sigma-Aldrich siRNA for OLFML3  
                     Sigma-Aldrich shRNA for OLFML3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for OLFML3
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020190
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020190
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_020190 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_020190

UniProtKB/Swiss-Prot: OLFL3_HUMAN, Q9NRN5
Function: Secreted scaffold protein that plays an essential role in dorsoventral patterning during
early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal
side. Acts by facilitating the association between the tolloid proteases and their substrate
chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have
matrix-related function involved in placental and embryonic development, or play a similar role in
other physiological processes

1 MGI mutant phenotype (inferred from 1 allele(MGI details for Olfml3):

normal

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



4 Interacting proteins for OLFML3 (Q9NRN52) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
CKMT2P175402MINT-63516
FEZ1Q996892MINT-65615
HSPB3Q129882MINT-64109
SETDB1Q150472MINT-65614
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007275 multicellular organismal development IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for OLFML3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OLFML3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020190

              Sigma-Aldrich siRNA for OLFML3  
                     Sigma-Aldrich shRNA for OLFML3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_020190  

REFSEQ mRNAs for OLFML3 gene: 

NM_020190.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_020190  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020190
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020190
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_020190 

Additional cDNA sequence: 

AF201945.1 AK075353.1 AK075369.1 AK075479.1 AK075544.1 AK092766.1 AK222471.1 AK223221.1 
AK297902.1 AY358954.1 AY464015.1 BC009920.2 CR593069.1 CR595134.1 CR596335.1 CR597657.1 
CR598507.1 CR598729.1 CR599952.1 CR601422.1 CR602607.1 CR602631.1 CR603678.1 CR606952.1 
CR608311.1 CR608635.1 CR610491.1 CR610535.1 CR610849.1 CR613617.1 CR615262.1 CR617511.1 
CR618552.1 CR618591.1 CR624364.1 CR625123.1 

19 DOTS entries:

DT.317653  DT.100673977  DT.100786765  DT.121415544  DT.100786757  DT.121415557  DT.97809428  DT.100023911 
DT.100786769  DT.121415563  DT.121415565  DT.100710650  DT.121415523  DT.100786759  DT.100786770  DT.121415535 
DT.121415541  DT.121415545  DT.100720197 

24/360 AceView cDNA sequences (see all 360 ):

BP341546 BP372763 BU855834 CR617511 CB216603 BP373755 BU848988 BP377486 
AA359689 BP379283 BU845799 AI903965 CR608311 AW304787 AA678300 CR598507 
CD671428 AY464015 AL554606 BP361355 BP371824 BP378996 CR598729 BP376784 

highest scoring ESTs for OLFML3:

AI078144 AA022654 AA046858 AA046991 AA091730 AA126284 AA216643 AA219570 AA249586 AA297653 

Unigene Cluster for OLFML3:

Olfactomedin-like 3
Hs.9315  [show with all ESTs]
Unigene Representative Sequence: AK075544


GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for OLFML3 (see all 6 )

ExUns: 1a · 1b ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b · 4c ^ 5a · 5b
SP1:                    -                             -               
SP2:              -     -                             -               
SP3:              -     -                                             
SP4:                                                                  
SP5:              -                                                   

About this scheme

ECgene alternative splicing isoforms for OLFML3

3 Ensembl transcripts including schematic representations:
ENST00000320334  ENST00000393300  ENST00000369551  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

OLFML3 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for OLFML3

1 / 2 / 3

3 probe-sets matching OLFML3 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
46694_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI078144 1.00 1.00 1.00 1

218162_at2, 3 U133-A 1 1.00 1.00 -- -- NM_020190 0.60 1.00 0.82 1

218162_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  OLFML3 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TCAACAAATT

SOURCE GeneReport for Unigene cluster: Hs.9315

UniProtKB/Swiss-Prot: OLFL3_HUMAN, Q9NRN5
Tissue specificity: Abundant in placenta, moderate in liver and heart, whereas fairly weak in other
tissues examined. On term placenta, mainly localized extracellularly surrounding the
syncytiotrophoblastic cells and very rarely expressed in the maternal decidua layer

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for OLFML3 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
OLFML31   -- olfactomedin-like 3 89.24(n)
96.31(a)
483126  XM_540242.2  XP_540242.1 
chimpanzee
(Pan troglodytes)
OLFML31   -- olfactomedin-like 3 99.75(n)
100(a)
457963  XM_514400.2  XP_514400.2 
cow
(Bos taurus)
OLFML31   -- olfactomedin-like 3 91.3(n)
97.04(a)
505318  NM_001075197.1  NP_001068665.1 
rat
(Rattus norvegicus)
Olfml31   -- olfactomedin-like 3 88.99(n)
95.07(a)
310743  XM_227535.3  XP_227535.3 
mouse
(Mus musculus)
Olfml31, 5 35
olfactomedin-like 31, 5 89(n)1
94.09(a)1
995431  NM_133859.21  NP_598620.21 
 AA9594365  AI1152095  (see all 13)
About this table        Species with no ortholog for OLFML3

ENSEMBL Gene Tree for OLFML3
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for OLFML3 gene
GLDN2  OLFM42  OLFML12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/41 NCBI SNPs in OLFML3 are shown (see all 41 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 62)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs20555421,2
A,C,F,H114326079(+) CATACG/AGAACT 1 -- ut31 ese37Minor allele frequency- A:0.03NA EA EU WA 672
rs73641,2
A,C,F,H114326184(+) GGATGC/ACCCAG 1 -- ut31 ese310Minor allele frequency- A:0.24MN EU EA WA NA 2430
rs67028331,2
A,C,F,H114323406(+) GAGGAG/CCCTAC 1 -- ng514Minor allele frequency- C:0.16EU EA WA 416
rs29383181,2
F,H114326244(+) GTGTTC/TTTCCC 1 -- ut31 trp34Minor allele frequency- T:0.01EU EA WA 418
--
rs115530811,2
H114325227(+) AATGAC/AACAGC 1 E/D mis1 ese34Minor allele frequency- A:0.00EU EA WA 404
--
rs283659071,2
F114323057(+) TATTAA/-TCATG 1 -- ng512Minor allele frequency- -:0.24MN EA 1252
rs37896141,2
C,F,H114324988(+) CAACCA/GCTGGG 1 -- int116Minor allele frequency- N:0.00EA NA EU WA 1470
rs127286791,2
C,F,H114323808(+) CGCCTA/GGAAGG 1 -- int18Minor allele frequency- G:0.01EU EA WA 840
rs22920941,2
C,F,H114324176(+) GAGACC/TTCTTC 1 -- int1 ese39Minor allele frequency- T:0.05EA EU WA 2334
--
rs725042281,2
--114323208(+) GAAAA-/AACCCCAC 1 -- ng510--------
About this table

HapMap Linkage Disequilibrium images for OLFML3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 610088

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/11 PubMed articles for OLFML3 gene (see all 11 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 56944 HGNC: 24956 AceView: OLFML3 Ensembl:ENSG00000116774 euGenes: HUgn56944
ECgene: OLFML3 H-InvDB: OLFML3
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for OLFML3:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Predesigned and custom siRNAs for OLFML3 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for OLFML3 Browse small molecules at Sigma-Aldrich
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